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Protein

Raftlin

Gene

Rftn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in protein trafficking via association with clathrin and AP2 complex (By similarity). Upon bacterial lipopolysaccharide stimulation, mediates internalization of TLR4 to endosomes in dendritic cells and macrophages, and internalization of poly(I:C) to TLR3-positive endosomes in myeloid dendritic cells and epithelial cells; resulting in activation of TICAM1-mediated signaling and subsequent IFNB1 production (PubMed:27022195). Involved in T-cell antigen receptor-mediated signaling by regulating tyrosine kinase LCK localization, T-cell dependent antibody production and cytokine secretion (PubMed:19414744). May regulate B-cell antigen receptor-mediated signaling (By similarity). May play a pivotal role in the formation and/or maintenance of lipid rafts (By similarity).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

  • B cell receptor signaling pathway Source: MGI
  • dsRNA transport Source: MGI
  • growth Source: MGI
  • interleukin-17 production Source: UniProtKB
  • membrane raft assembly Source: MGI
  • protein localization to membrane raft Source: UniProtKB
  • protein transport into membrane raft Source: UniProtKB
  • response to exogenous dsRNA Source: MGI
  • T cell antigen processing and presentation Source: UniProtKB
  • T cell receptor signaling pathway Source: UniProtKB
  • toll-like receptor 3 signaling pathway Source: MGI

Names & Taxonomyi

Protein namesi
Recommended name:
Raftlin
Alternative name(s):
Raft-linking protein
Gene namesi
Name:Rftn1
Synonyms:Kiaa0084
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1923688 Rftn1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant mice have smaller spleen, fewer splenocytes, reduced IgM production and secrete less IFNG, during antigen re-stimulation (PubMed:19414744). Double RFTN1 and RFTN2 mutant mice show no visible phenotype under pathogen-free conditions but show greatly reduced IFNB1 production in splenic dendritic cells following poly(I:C) or LPS stimulation (PubMed:27022195).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002519542 – 554RaftlinAdd BLAST553

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Lipidationi3S-palmitoyl cysteineBy similarity1
Modified residuei183PhosphoserineCombined sources1
Modified residuei199PhosphoserineBy similarity1
Modified residuei507PhosphoserineCombined sources1
Modified residuei530PhosphoserineCombined sources1

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Proteomic databases

EPDiQ6A0D4
MaxQBiQ6A0D4
PaxDbiQ6A0D4
PeptideAtlasiQ6A0D4
PRIDEiQ6A0D4

PTM databases

iPTMnetiQ6A0D4
PhosphoSitePlusiQ6A0D4
SwissPalmiQ6A0D4

Expressioni

Tissue specificityi

Expressed in T-cells, B-cells, thymus and spleen (at protein level) (PubMed:19414744, PubMed:12805216). Expressed in dendritic cells, macrophages, heart, lung and small intestine (PubMed:19414744).2 Publications

Gene expression databases

BgeeiENSMUSG00000039316
CleanExiMM_RFTN1
ExpressionAtlasiQ6A0D4 baseline and differential
GenevisibleiQ6A0D4 MM

Interactioni

Subunit structurei

Interacts with TLR4; the interaction occurs in response to lipopolysaccharide stimulation. Interacts with CLTC; the interaction occurs in response to pathogens. Interacts with AP2A1 and AP2B1.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000046524

Structurei

3D structure databases

ProteinModelPortaliQ6A0D4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the raftlin family.Curated

Phylogenomic databases

eggNOGiENOG410IETJ Eukaryota
ENOG410ZCAE LUCA
GeneTreeiENSGT00530000063609
HOGENOMiHOG000121785
HOVERGENiHBG093894
InParanoidiQ6A0D4
OMAiVEQWTVL
OrthoDBiEOG091G07W6
PhylomeDBiQ6A0D4
TreeFamiTF333285

Family and domain databases

InterProiView protein in InterPro
IPR028169 Raftlin
PANTHERiPTHR17601 PTHR17601, 1 hit
PfamiView protein in Pfam
PF15250 Raftlin, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6A0D4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGCSLNKLEK REEKRPGNIY STLKRPQVET KVDVTYEYCF LEFTTLTAAE
60 70 80 90 100
LPRSSATRLA SLRDLPDQLL ELYQQGFSLA ALHPFVQPTR RQEKILLEHI
110 120 130 140 150
FRAILVKKTN RSQKAELHDE GYTLELDYCS SLEHLADQKL IPEFIKKVQE
160 170 180 190 200
AASQGLKFVS VVPQYQPSVS SAGSRRLKPV ANSVEDARDV KCTLGDRSSL
210 220 230 240 250
ENDTPKAAET DAATAGVNRR PETKPGSTGD VPSAQQPGIP SPSAENEAGE
260 270 280 290 300
FPLRGLQPAL DRSEGDPSNG PEELPSRKME IFAFFNRPKS QQKCRQYYPV
310 320 330 340 350
TIPLQVSKNG QTVSSLDASW LEHMSDHFRK GGVLVNAVFQ LGMANDSFYG
360 370 380 390 400
LTDGVFIFEA VSTEDNRTTQ GYDAIVVEQW TVLEGTEVQT DYMPLLNSLA
410 420 430 440 450
AYGWQLTCVL PTPILKTTRE GNVSTKQIVF LQRPCLPQKT KKRESKFQWR
460 470 480 490 500
FSRNEIHGRQ TRKSKGKLSA SNKQQAEENE KNLEDQFSKA GDVGNCVLGA
510 520 530 540 550
PQWGRASEVR EQRQGSAAVQ NGPAGHNRDS VALRHSNPRA EAELAAGPTP

TEAN
Length:554
Mass (Da):61,537
Last modified:January 23, 2007 - v4
Checksum:i34AFE53D91861A2C
GO

Sequence cautioni

The sequence BAD32162 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAE31871 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti236Q → R in BAE30971 (PubMed:16141072).Curated1
Sequence conflicti236Q → R in BAE31871 (PubMed:16141072).Curated1
Sequence conflicti472N → D in BAD32162 (PubMed:15368895).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK172884 mRNA Translation: BAD32162.1 Different initiation.
AK152130 mRNA Translation: BAE30971.1
AK153288 mRNA Translation: BAE31871.1 Different initiation.
AK155870 mRNA Translation: BAE33473.1
BC052074 mRNA Translation: AAH52074.1
CCDSiCCDS28873.1
RefSeqiNP_852062.1, NM_181397.2
UniGeneiMm.41854

Genome annotation databases

EnsembliENSMUST00000044503; ENSMUSP00000046524; ENSMUSG00000039316
GeneIDi76438
KEGGimmu:76438
UCSCiuc008cyr.1 mouse

Similar proteinsi

Entry informationi

Entry nameiRFTN1_MOUSE
AccessioniPrimary (citable) accession number: Q6A0D4
Secondary accession number(s): Q3U654, Q3U8P6, Q80US1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 94 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health