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Protein

Cell division cycle 5-like protein

Gene

Cdc5l

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-binding protein involved in cell cycle control. May act as a transcription activator. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi31 – 5424H-T-H motifPROSITE-ProRule annotationAdd
BLAST
DNA bindingi82 – 10423H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Cell cycle, DNA damage, DNA repair, mRNA processing, mRNA splicing, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division cycle 5-like protein
Alternative name(s):
Cdc5-like protein
Gene namesi
Name:Cdc5l
Synonyms:Kiaa0432
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1918952. Cdc5l.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 802802Cell division cycle 5-like proteinPRO_0000197092Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki219 – 219Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei227 – 2271PhosphothreonineBy similarity
Modified residuei303 – 3031PhosphoserineBy similarity
Modified residuei358 – 3581PhosphoserineBy similarity
Modified residuei377 – 3771PhosphothreonineCombined sources
Modified residuei385 – 3851PhosphothreonineCombined sources
Modified residuei396 – 3961PhosphothreonineCombined sources
Modified residuei404 – 4041PhosphothreonineCombined sources
Modified residuei411 – 4111PhosphothreonineBy similarity
Modified residuei415 – 4151PhosphothreonineCombined sources
Modified residuei417 – 4171PhosphoserineBy similarity
Modified residuei424 – 4241PhosphothreonineCombined sources
Modified residuei430 – 4301PhosphothreonineBy similarity
Modified residuei438 – 4381PhosphothreonineCombined sources
Modified residuei442 – 4421PhosphothreonineCombined sources

Post-translational modificationi

Phosphorylated on serine and threonine residues. Phosphorylation on Thr-411 and Thr-438 is required for CDC5L-mediated mRNA splicing. Has no effect on subcellular location nor on homodimerization. Phosphorylated in vitro by CDK2. Phosphorylation enhances interaction with PPP1R8.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ6A068.
MaxQBiQ6A068.
PaxDbiQ6A068.
PRIDEiQ6A068.

PTM databases

iPTMnetiQ6A068.
PhosphoSiteiQ6A068.

Expressioni

Gene expression databases

BgeeiQ6A068.
CleanExiMM_CDC5L.
ExpressionAtlasiQ6A068. baseline and differential.
GenevisibleiQ6A068. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with DAPK3. Belongs to the spliceosome complex. Part of a spliceosomal 'core' complex consisting of CDC5L, PLRG1, SPF27, CCAP1, CCAP3 and CCAP6. Interacts with PLRG1, Lodestar/TTF2, and NIPP1/PPP1R8. Identified in the spliceosome C complex. Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts (via its C-terminus) directly in the complex with PRPF19 and BCAS2. Interacts (via its C-terminus) directly with PRGL1 (via its WD40 repeat domain); the interaction is required for mRNA splicing but not for spliceosome assembly. Also interacts with CTNNBL1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi214866. 19 interactions.
IntActiQ6A068. 18 interactions.
MINTiMINT-4117023.
STRINGi10090.ENSMUSP00000024727.

Structurei

3D structure databases

ProteinModelPortaliQ6A068.
SMRiQ6A068. Positions 10-118.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 5656HTH myb-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini57 – 10852HTH myb-type 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni200 – 2067Required for interaction with CTNNBL1By similarity
Regioni260 – 606347Interaction with PPP1R8By similarityAdd
BLAST
Regioni501 – 659159Interaction with DAPK3By similarityAdd
BLAST
Regioni706 – 80095Interaction with PLRG1By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili142 – 245104Sequence analysisAdd
BLAST
Coiled coili676 – 70126Sequence analysisAdd
BLAST
Coiled coili764 – 80239Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi165 – 271107Nuclear localization signalSequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the CEF1 family.Curated
Contains 2 HTH myb-type DNA-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0050. Eukaryota.
COG5147. LUCA.
GeneTreeiENSGT00550000074922.
HOGENOMiHOG000182446.
HOVERGENiHBG052766.
InParanoidiQ6A068.
KOiK12860.
OMAiNPEESMG.
OrthoDBiEOG7JDQWW.
PhylomeDBiQ6A068.
TreeFamiTF101061.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
InterProiIPR021786. Cdc5p/Cef1.
IPR009057. Homeodomain-like.
IPR017930. Myb_dom.
IPR001005. SANT/Myb.
[Graphical view]
PfamiPF11831. Myb_Cef. 1 hit.
[Graphical view]
SMARTiSM00717. SANT. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
PROSITEiPS51294. HTH_MYB. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6A068-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRIMIKGGV WRNTEDEILK AAVMKYGKNQ WSRIASLLHR KSAKQCKARW
60 70 80 90 100
YEWLDPSIKK TEWSREEEEK LLHLAKLMPT QWRTIAPIIG RTAAQCLEHY
110 120 130 140 150
EFLLDKTAQR DNEEETTDDP RKLKPGEIDP NPETKPARPD PIDMDEDELE
160 170 180 190 200
MLSEARARLA NTQGKKAKRK AREKQLEEAR RLAALQKRRE LRAAGIEIQK
210 220 230 240 250
KRKKKRGVDY NAEIPFEKKP ALGFYDTSEE NYQALDADFR KLRQQDLDGE
260 270 280 290 300
LRSEKEGRDR KKDKQHLKRK KESDLPSAIL QTSGVSEFTK KRSKLVLPAP
310 320 330 340 350
QISDAELQEV VKVGQASEVA RQTAEESGIT NSASSTLLSE YNVTNNSIAL
360 370 380 390 400
RTPRTPASQD RILQEAQNLM ALTNVDTPLK GGLNTPLHES DFSGVTPQRQ
410 420 430 440 450
VVQTPNTVLS TPFRTPSNGA EGLTPRSGTT PKPVTNATPG RTPLRDKLNI
460 470 480 490 500
NPEDGMADYS DPSYVKQMER ESREHLRLGL LGLPAPKNDF EIVLPENAEK
510 520 530 540 550
ELEEREIDDT YIEDAADVDA RKQAIRDAER VKEMKRMHKA VQKDLPRPSE
560 570 580 590 600
VNETILRPLN VEPPLTDLQK SEELIKKEMI TMLHYDLLHH PYEPSGNKKG
610 620 630 640 650
KNVGFATNNS EHITYLEHSP YEKFSKEDLK KAQDALVQEM EVVKQGMSHG
660 670 680 690 700
ELSSEAYNQV WEECYSQVLY LPAQSRYTRA NLASKKDRIE SLEKRLEINR
710 720 730 740 750
GHMTTEAKRA AKMEKKMKIL LGGYQSRAMG LMKQLNDLWD QIEQAHLELR
760 770 780 790 800
TFEELKKHED SAIPRRLECL KEDVQRQQER EKELQQRYAD LLMEKETLQA

KF
Length:802
Mass (Da):92,190
Last modified:August 16, 2005 - v2
Checksum:i7EFFBC4A1B7ABA3F
GO

Sequence cautioni

The sequence BAD32228.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK172950 mRNA. Translation: BAD32228.1. Different initiation.
BC031480 mRNA. Translation: AAH31480.1.
CCDSiCCDS37627.1.
RefSeqiNP_690023.1. NM_152810.2.
UniGeneiMm.28270.

Genome annotation databases

EnsembliENSMUST00000024727; ENSMUSP00000024727; ENSMUSG00000023932.
GeneIDi71702.
KEGGimmu:71702.
UCSCiuc008cqj.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK172950 mRNA. Translation: BAD32228.1. Different initiation.
BC031480 mRNA. Translation: AAH31480.1.
CCDSiCCDS37627.1.
RefSeqiNP_690023.1. NM_152810.2.
UniGeneiMm.28270.

3D structure databases

ProteinModelPortaliQ6A068.
SMRiQ6A068. Positions 10-118.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214866. 19 interactions.
IntActiQ6A068. 18 interactions.
MINTiMINT-4117023.
STRINGi10090.ENSMUSP00000024727.

PTM databases

iPTMnetiQ6A068.
PhosphoSiteiQ6A068.

Proteomic databases

EPDiQ6A068.
MaxQBiQ6A068.
PaxDbiQ6A068.
PRIDEiQ6A068.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000024727; ENSMUSP00000024727; ENSMUSG00000023932.
GeneIDi71702.
KEGGimmu:71702.
UCSCiuc008cqj.2. mouse.

Organism-specific databases

CTDi988.
MGIiMGI:1918952. Cdc5l.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0050. Eukaryota.
COG5147. LUCA.
GeneTreeiENSGT00550000074922.
HOGENOMiHOG000182446.
HOVERGENiHBG052766.
InParanoidiQ6A068.
KOiK12860.
OMAiNPEESMG.
OrthoDBiEOG7JDQWW.
PhylomeDBiQ6A068.
TreeFamiTF101061.

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

PROiQ6A068.
SOURCEiSearch...

Gene expression databases

BgeeiQ6A068.
CleanExiMM_CDC5L.
ExpressionAtlasiQ6A068. baseline and differential.
GenevisibleiQ6A068. MM.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
InterProiIPR021786. Cdc5p/Cef1.
IPR009057. Homeodomain-like.
IPR017930. Myb_dom.
IPR001005. SANT/Myb.
[Graphical view]
PfamiPF11831. Myb_Cef. 1 hit.
[Graphical view]
SMARTiSM00717. SANT. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
PROSITEiPS51294. HTH_MYB. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
    DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pancreatic islet.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  3. Lubec G., Kang S.U., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 34-40 AND 766-771, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6J and OF1.
    Tissue: Brain and Hippocampus.
  4. "Evidence that Myb-related CDC5 proteins are required for pre-mRNA splicing."
    Burns C.G., Ohi R., Krainer A.R., Gould K.L.
    Proc. Natl. Acad. Sci. U.S.A. 96:13789-13794(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-377; THR-385; THR-396; THR-404; THR-415; THR-424; THR-438 AND THR-442, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Heart, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiCDC5L_MOUSE
AccessioniPrimary (citable) accession number: Q6A068
Secondary accession number(s): Q8K1J9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: August 16, 2005
Last modified: June 8, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.