Cell division cycle 5-like protein - Mus musculus (Mouse)

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Q6A068 (CDC5L_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 97. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cell division cycle 5-like protein

Alternative name(s):
Cdc5-like protein

Gene names

Name:	Cdc5l
Synonyms:	Kiaa0432

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	802 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA-binding protein involved in cell cycle control. May act as a transcription activator. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing By similarity.
Subunit structure	Homodimer By similarity. Interacts with DAPK3. Binds DNA. Binds to adeno-pre-mRNA in an ATP-stimulated manner. Belongs to the spliceosome complex. Part of a spliceosomal 'core' complex consisting of CDC5L, PLRG1, SPF27, CCAP1, CCAP3 and CCAP6. Interacts with PLRG1, Lodestar/TTF2, and NIPP1/PPP1R8. Identified in the spliceosome C complex. Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PTP4A1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts (via its C-terminus) directly in the complex with PRPF19 and BCAS2. Interacts (via its C-terminus) directly with PRGL1 (via its WD40 repeat domain); the interaction is required for mRNA splicing but not for spliceosome assembly. Also interacts with CTNNBL1 By similarity.
Subcellular location	Nucleus. Nucleus speckle By similarity. Cytoplasm By similarity. Note: May shuttle between cytoplasm and nucleus By similarity. Ref.4
Post-translational modification	Phosphorylated on serine and threonine residues. Phosphorylation on Thr-411 and Thr-438 is required for CDC5L-mediated mRNA splicing. Has no effect on subcellular location nor on homodimerization. Phosphorylated in vitro by CDK2. Phosphorylation enhances interaction with PPP1R8 By similarity.
Sequence similarities	Belongs to the CEF1 family. Contains 2 HTH myb-type DNA-binding domains.
Sequence caution	The sequence BAD32228.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Cell cycle Transcription Transcription regulation mRNA processing mRNA splicing
Cellular component	Cytoplasm Nucleus Spliceosome
Domain	Coiled coil Repeat
Ligand	DNA-binding RNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW mRNA splicing, via spliceosome Inferred from sequence or structural similarity. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Prp19 complex Inferred from sequence or structural similarity. Source: UniProtKB catalytic step 2 spliceosome Inferred from electronic annotation. Source: Compara cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear speck Inferred from electronic annotation. Source: UniProtKB-SubCell nucleolus Inferred from electronic annotation. Source: Compara
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW WD40-repeat domain binding Inferred from sequence or structural similarity. Source: UniProtKB chromatin binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 802

802

Cell division cycle 5-like protein

PRO_0000197092

Regions

Domain

1 – 56

HTH myb-type 1

Domain

57 – 108

HTH myb-type 2

DNA binding

31 – 54

H-T-H motif By similarity

DNA binding

82 – 104

H-T-H motif By similarity

Region

165 – 271

107

Nuclear localization signal Potential

Region

200 – 206

Required for interaction with CTNNBL1 By similarity

Region

260 – 606

347

Interaction with PPP1R8 By similarity

Region

501 – 659

159

Interaction with DAPK3 By similarity

Region

706 – 800

Interaction with PLRG1 By similarity

Coiled coil

142 – 245

104

Potential

Coiled coil

676 – 701

Potential

Coiled coil

764 – 802

Potential

Amino acid modifications

Modified residue

227

Phosphothreonine By similarity

Modified residue

303

Phosphoserine By similarity

Modified residue

358

Phosphoserine By similarity

Modified residue

377

Phosphothreonine By similarity

Modified residue

385

Phosphothreonine Ref.5 Ref.6

Modified residue

396

Phosphothreonine Ref.5

Modified residue

404

Phosphothreonine By similarity

Modified residue

411

Phosphothreonine By similarity

Modified residue

415

Phosphothreonine By similarity

Modified residue

417

Phosphoserine By similarity

Modified residue

424

Phosphothreonine By similarity

Modified residue

430

Phosphothreonine By similarity

Modified residue

438

Phosphothreonine By similarity

Modified residue

442

Phosphothreonine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q6A068 [UniParc].

Last modified August 16, 2005. Version 2.
Checksum: 7EFFBC4A1B7ABA3F
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FASTA

802

92,190

        10         20         30         40         50         60 
MPRIMIKGGV WRNTEDEILK AAVMKYGKNQ WSRIASLLHR KSAKQCKARW YEWLDPSIKK 

        70         80         90        100        110        120 
TEWSREEEEK LLHLAKLMPT QWRTIAPIIG RTAAQCLEHY EFLLDKTAQR DNEEETTDDP 

       130        140        150        160        170        180 
RKLKPGEIDP NPETKPARPD PIDMDEDELE MLSEARARLA NTQGKKAKRK AREKQLEEAR 

       190        200        210        220        230        240 
RLAALQKRRE LRAAGIEIQK KRKKKRGVDY NAEIPFEKKP ALGFYDTSEE NYQALDADFR 

       250        260        270        280        290        300 
KLRQQDLDGE LRSEKEGRDR KKDKQHLKRK KESDLPSAIL QTSGVSEFTK KRSKLVLPAP 

       310        320        330        340        350        360 
QISDAELQEV VKVGQASEVA RQTAEESGIT NSASSTLLSE YNVTNNSIAL RTPRTPASQD 

       370        380        390        400        410        420 
RILQEAQNLM ALTNVDTPLK GGLNTPLHES DFSGVTPQRQ VVQTPNTVLS TPFRTPSNGA 

       430        440        450        460        470        480 
EGLTPRSGTT PKPVTNATPG RTPLRDKLNI NPEDGMADYS DPSYVKQMER ESREHLRLGL 

       490        500        510        520        530        540 
LGLPAPKNDF EIVLPENAEK ELEEREIDDT YIEDAADVDA RKQAIRDAER VKEMKRMHKA 

       550        560        570        580        590        600 
VQKDLPRPSE VNETILRPLN VEPPLTDLQK SEELIKKEMI TMLHYDLLHH PYEPSGNKKG 

       610        620        630        640        650        660 
KNVGFATNNS EHITYLEHSP YEKFSKEDLK KAQDALVQEM EVVKQGMSHG ELSSEAYNQV 

       670        680        690        700        710        720 
WEECYSQVLY LPAQSRYTRA NLASKKDRIE SLEKRLEINR GHMTTEAKRA AKMEKKMKIL 

       730        740        750        760        770        780 
LGGYQSRAMG LMKQLNDLWD QIEQAHLELR TFEELKKHED SAIPRRLECL KEDVQRQQER 

       790        800 
EKELQQRYAD LLMEKETLQA KF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H. DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreatic islet.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary gland.
[3]	Lubec G., Kang S.U., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 34-40 AND 766-771, MASS SPECTROMETRY. Strain: C57BL/6 and OF1. Tissue: Brain and Hippocampus.
[4]	"Evidence that Myb-related CDC5 proteins are required for pre-mRNA splicing." Burns C.G., Ohi R., Krainer A.R., Gould K.L. Proc. Natl. Acad. Sci. U.S.A. 96:13789-13794(1999) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[5]	"Identification of phosphoproteins and their phosphorylation sites in the WEHI-231 B lymphoma cell line." Shu H., Chen S., Bi Q., Mumby M., Brekken D.L. Mol. Cell. Proteomics 3:279-286(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-385 AND THR-396, MASS SPECTROMETRY. Tissue: B-cell lymphoma.
[6]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-385, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK172950 mRNA. Translation: BAD32228.1. Different initiation. BC031480 mRNA. Translation: AAH31480.1.
IPI	IPI00284444.
RefSeq	NP_690023.1. NM_152810.2.
UniGene	Mm.28270.
3D structure databases
ProteinModelPortal	Q6A068.
SMR	Q6A068. Positions 10-118.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6A068. 17 interactions.
MINT	MINT-4117023.
PTM databases
PhosphoSite	Q6A068.
Proteomic databases
PaxDb	Q6A068.
PRIDE	Q6A068.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000024727; ENSMUSP00000024727; ENSMUSG00000023932.
GeneID	71702.
KEGG	mmu:71702.
UCSC	uc008cqj.2. mouse.
Organism-specific databases
CTD	988.
MGI	MGI:1918952. Cdc5l.
Rouge	Search...
Phylogenomic databases
eggNOG	COG5147.
GeneTree	ENSGT00550000074922.
HOGENOM	HOG000182446.
HOVERGEN	HBG052766.
InParanoid	Q6A068.
KO	K12860.
OMA	HIAYLEH.
OrthoDB	EOG48GW2S.
Gene expression databases
ArrayExpress	Q6A068.
Bgee	Q6A068.
CleanEx	MM_CDC5L.
Genevestigator	Q6A068.
GermOnline	ENSMUSG00000023932. Mus musculus.
Family and domain databases
Gene3D	1.10.10.60. 2 hits.
InterPro	IPR021786. DUF3351. IPR009057. Homeodomain-like. IPR017930. Myb_dom. IPR001005. SANT/Myb. [Graphical view]
Pfam	PF11831. Myb_Cef. 1 hit. [Graphical view]
SMART	SM00717. SANT. 2 hits. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS51294. HTH_MYB. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	334267.
SOURCE	Search...

Entry information

Entry name

CDC5L_MOUSE

Accession

Primary (citable) accession number: Q6A068
Secondary accession number(s): Q8K1J9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	August 16, 2005
Last modified:	May 29, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents