Q6A065 (CE170_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Centrosomal protein of 170 kDa
Short name=Cep170

Gene names

Name:	Cep170
Synonyms:	Kiaa0470

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1588 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in microtubule organization By similarity.
Subunit structure	Interacts with PLK1 By similarity.
Subcellular location	Cytoplasm › cytoskeleton › centrosome › centriole By similarity. Cytoplasm › cytoskeleton › spindle By similarity. Note: Associated with the mature mother centriole By similarity. Associated with spindle microtubules during mitosis By similarity.
Post-translational modification	Phosphorylated; probably by PLK1.
Sequence similarities	Belongs to the CEP170 family. Contains 1 FHA domain.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Microtubule
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	centriole Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule Inferred from electronic annotation. Source: UniProtKB-KW spindle Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6A065-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6A065-2)

The sequence of this isoform differs from the canonical sequence as follows:
     211-236: CSTEAKHVEGQSAAASEEALFPFCRE → KNTSVAVASNSYWASIYSLNKSHSTL
     237-1588: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q6A065-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1257: Missing.
     1344-1353: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1588	1588	Centrosomal protein of 170 kDa		PRO_0000282888
Regions
	Domain	23 – 73	51	FHA
	Region	844 – 1588	745	Targeting to microtubules By similarity
	Region	1103 – 1588	486	Targeting to centrosomes By similarity
	Coiled coil	1467 – 1495	29	Potential
Amino acid modifications
	Modified residue	355	1	Phosphoserine Ref.5
	Modified residue	358	1	Phosphoserine Ref.5 Ref.6
	Modified residue	363	1	Phosphotyrosine By similarity
	Modified residue	443	1	Phosphoserine Ref.7
	Modified residue	494	1	Phosphoserine By similarity
	Modified residue	498	1	Phosphothreonine By similarity
	Modified residue	628	1	Phosphoserine By similarity
	Modified residue	631	1	Phosphoserine By similarity
	Modified residue	829	1	Phosphoserine Ref.7
	Modified residue	922	1	Phosphoserine By similarity
	Modified residue	925	1	Phosphoserine By similarity
	Modified residue	950	1	Phosphoserine By similarity
	Modified residue	1102	1	Phosphoserine Ref.6
	Modified residue	1104	1	Phosphoserine By similarity
	Modified residue	1150	1	Phosphoserine By similarity
	Modified residue	1155	1	Phosphoserine Ref.6
	Modified residue	1188	1	Phosphoserine Ref.6
	Modified residue	1241	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 1257	1257	Missing in isoform 3.		VSP_024243
	Alternative sequence	211 – 236	26	CSTEA…PFCRE → KNTSVAVASNSYWASIYSLN KSHSTL in isoform 2.		VSP_024244
	Alternative sequence	237 – 1588	1352	Missing in isoform 2.		VSP_024245
	Alternative sequence	1344 – 1353	10	Missing in isoform 3.		VSP_024246

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 3, 2007. Version 2. Checksum: B7140E6F82AF93A7	FASTA	1,588	175,050
	10 20 30 40 50 60 MSLTSWFLVS SGGTRHRLPR EMIFVGRDDC ELMLQSRSVD KQHAVINYDA SMDEHLVKDL 70 80 90 100 110 120 GSLNGTFVND VRIPEQTYIT LKLEDKLRFG YDTNLFTVVR GEMRVPEEAL KHEKFTIQLQ 130 140 150 160 170 180 LSQKSSESEL PKSASAKGTD SKVEAAAEVQ PRATEALKSE EKPMDVSAMP RGTPLYGQPS 190 200 210 220 230 240 WWGDAEEDEQ RAFKANGKPE GKSQEAGASG CSTEAKHVEG QSAAASEEAL FPFCREPSYF 250 260 270 280 290 300 EIPTKEFQQP SQIAESTIHE IPTKDTPSSH TAGAGHASFT IEFDDSTPGK VTIRDHVTKF 310 320 330 340 350 360 TSDQRHKSKK ASPGTQDLPG IQTGMMAPEN KVADWLAQNN PPQMVWERTE EDSKSIKSDV 370 380 390 400 410 420 PVYLKRLKGN KHDDGTQSDS ENAGAHRRCS KRATLEEHLR RHHSEQKKKA QSTEKHQEQA 430 440 450 460 470 480 ATSSTHHRGG HGVPHGKLLK QKSEEPSVSL PFLQTALLRS SGSLGHRPSQ EMDVMLKNQA 490 500 510 520 530 540 TSASSEKDND DDQSDKGTYT IELENPNSEE VEARKMIDKV FGVDDNQDYN RPIINEKHKG 550 560 570 580 590 600 LIKDWALNSA AVVMEERKPL STPGFHNSEE AISSSGSKRW VSQWASLAAN HTRHDPEERL 610 620 630 640 650 660 MELSATVENE TDTGDAGVSL RSTSCTTSLA SQGERKRRTL PQLPNEEKLL ESSRAKVVPQ 670 680 690 700 710 720 RSEIGEKQDT ELQEKEAQVY QSEKHDADRG LSKMSRAVNG ESPKTGGDGK ALLHSGSSSS 730 740 750 760 770 780 KEKSETEKET SLVKQTLAKM QQQEQKEQAQ WTPTKFPSKN ALGHIDKCRE ESSKQESQLL 790 800 810 820 830 840 EKVSGHSTSK GDRVIQNESK RRKAEEIPKC QASKGDKKES SKSLVRQGSF TIDKPSSNIP 850 860 870 880 890 900 IELIPHINKQ NSSVPTALAL TSASRLRERS DSLDTDSSMD TTLILKDTEA VMAFLEAKLR 910 920 930 940 950 960 EDNNKTDEGP DTPSYNRDNS ISPESDVDTA STISLVTGET ERKSTQKRKS FTSLYKDRCS 970 980 990 1000 1010 1020 TSSPSKDVTK SGSREKIEKK AKSRSADIGA RADGRKFVQS SGRIRQPSID LTDDDQTSSV 1030 1040 1050 1060 1070 1080 PHSAISDIMS SDQETYSCKS HGRTPLTSAD EHNIHSKLEG GKATKSKTSP VASGSTSKST 1090 1100 1110 1120 1130 1140 TLPRPRPTRT SLLRRARLGE ASDSELADAD KASVASEVST TSSTSKPPTG RRTISRIDLL 1150 1160 1170 1180 1190 1200 AQPRRTRLGS LSARSDSEAT ISRSSASART AEAVIRSGAR LVPSDKLSPR TRANSISRLS 1210 1220 1230 1240 1250 1260 DSKVKSMSST HGSPSVNSRW RRFPTDYAST SEDEFGSNRN SPKHTRLRTS PALKTTRMQS 1270 1280 1290 1300 1310 1320 TGSAMPASSS FKHRIKEQED YIRDWTAHRE EIARISQDLA LIAREINDVA GEIDSVTSSG 1330 1340 1350 1360 1370 1380 TAPSTTVSTA ATTPGSAIDT REEVGDLHGE MHKLVDRVFD ESLNFRKIPP LVHSKTPEGN 1390 1400 1410 1420 1430 1440 NGRSVDSRPQ PAEHPDHLTI TRRRTWSRDE VMGDNLLLSS VFQFSRKIRQ SIDKTAGKIR 1450 1460 1470 1480 1490 1500 ILFKDKDRNW DDIENKLRAE SEVPIVKTSS MEISSILQEL KRVEKQLQVI NAMIDPDGTL 1510 1520 1530 1540 1550 1560 EALNNMGFPN AILPSPPKQK SSPVNNHSSP SQTPALCPPE TRALHPAAAG VAAAASTEFE 1570 1580 NAESEADFSI HFNRFNPDGE EEDVTVHE
	Isoform 2 [UniParc]. Checksum: 316C395EA550A7AA	FASTA	236	26,206
	10 20 30 40 50 60 MSLTSWFLVS SGGTRHRLPR EMIFVGRDDC ELMLQSRSVD KQHAVINYDA SMDEHLVKDL 70 80 90 100 110 120 GSLNGTFVND VRIPEQTYIT LKLEDKLRFG YDTNLFTVVR GEMRVPEEAL KHEKFTIQLQ 130 140 150 160 170 180 LSQKSSESEL PKSASAKGTD SKVEAAAEVQ PRATEALKSE EKPMDVSAMP RGTPLYGQPS 190 200 210 220 230 WWGDAEEDEQ RAFKANGKPE GKSQEAGASG KNTSVAVASN SYWASIYSLN KSHSTL
	Isoform 3 [UniParc]. Checksum: 92A5A8D7B504F9E6	FASTA	321	35,531
	10 20 30 40 50 60 MQSTGSAMPA SSSFKHRIKE QEDYIRDWTA HREEIARISQ DLALIAREIN DVAGEIDSVT 70 80 90 100 110 120 SSGTAPSTTV STAATTPGSA IDTREELVDR VFDESLNFRK IPPLVHSKTP EGNNGRSVDS 130 140 150 160 170 180 RPQPAEHPDH LTITRRRTWS RDEVMGDNLL LSSVFQFSRK IRQSIDKTAG KIRILFKDKD 190 200 210 220 230 240 RNWDDIENKL RAESEVPIVK TSSMEISSIL QELKRVEKQL QVINAMIDPD GTLEALNNMG 250 260 270 280 290 300 FPNAILPSPP KQKSSPVNNH SSPSQTPALC PPETRALHPA AAGVAAAAST EFENAESEAD 310 320 FSIHFNRFNP DGEEEDVTVH E

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1462-1588 (ISOFORMS 1/3). Strain: C57BL/6J. Tissue: Muellerian duct and Testis.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6. Tissue: Brain.
[4]	"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H. DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 786-1588 (ISOFORM 1).
[5]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-355 AND SER-358, MASS SPECTROMETRY. Tissue: Melanoma.
[6]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-358; SER-1102; SER-1155 AND SER-1188, MASS SPECTROMETRY. Tissue: Macrophage.
[7]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-443 AND SER-829, MASS SPECTROMETRY. Tissue: Embryonic fibroblast.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK016937 mRNA. Translation: BAB30507.2. AK078541 mRNA. Translation: BAC37328.1. AC113311 Genomic DNA. No translation available. BC054781 mRNA. Translation: AAH54781.1. BC057019 mRNA. Translation: AAH57019.1. AK172953 mRNA. Translation: BAD32231.1.
IPI	IPI00407623. IPI00620148. IPI00667973.
UniGene	Mm.269991.
3D structure databases
ProteinModelPortal	Q6A065.
SMR	Q6A065. Positions 13-98.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6A065. 14 interactions.
STRING	10090.ENSMUSP00000059562.
PTM databases
PhosphoSite	Q6A065.
Proteomic databases
PaxDb	Q6A065.
PRIDE	Q6A065.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	uc007dub.1. mouse. uc007duc.1. mouse. uc007dug.1. mouse.
Organism-specific databases
MGI	MGI:1918348. Cep170.
Rouge	Search...
Phylogenomic databases
eggNOG	NOG18196.
HOGENOM	HOG000111524.
HOVERGEN	HBG058059.
InParanoid	Q6A065.
OrthoDB	EOG4BCDMF.
Gene expression databases
ArrayExpress	Q6A065.
Bgee	Q6A065.
CleanEx	MM_CEP170.
Genevestigator	Q6A065.
Family and domain databases
Gene3D	2.60.200.20. 1 hit.
InterPro	IPR026644. CEP170. IPR000253. FHA_dom. IPR008984. SMAD_FHA_domain. [Graphical view]
PANTHER	PTHR15715:SF2. PTHR15715:SF2. 1 hit.
Pfam	PF00498. FHA. 1 hit. [Graphical view]
SMART	SM00240. FHA. 1 hit. [Graphical view]
SUPFAM	SSF49879. SMAD_FHA. 1 hit.
PROSITE	PS50006. FHA_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CEP170. mouse.
SOURCE	Search...

Entry information

Entry name

CE170_MOUSE

Accession

Primary (citable) accession number: Q6A065
Secondary accession number(s): Q7TQD9, Q8BJW2, Q9D3Z0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	April 3, 2007
Last modified:	May 1, 2013
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program