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Protein

Switch-associated protein 70

Gene

Swap70

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion (By similarity).By similarity

GO - Molecular functioni

  • ATP binding Source: MGI
  • cadherin binding Source: MGI
  • DNA binding Source: MGI

GO - Biological processi

  • actin filament bundle assembly Source: CACAO
  • isotype switching Source: MGI
  • negative regulation of actin filament depolymerization Source: CACAO
  • negative regulation of cell-cell adhesion mediated by integrin Source: CACAO
  • negative regulation of peptidyl-serine dephosphorylation Source: CACAO
  • positive regulation of actin filament bundle assembly Source: CACAO
  • positive regulation of cytosolic calcium ion concentration Source: CACAO
  • positive regulation of mast cell chemotaxis Source: CACAO
  • regulation of actin polymerization or depolymerization Source: CACAO
  • regulation of protein localization Source: CACAO
  • somatic cell DNA recombination Source: MGI

Keywordsi

Molecular functionDNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Switch-associated protein 70
Short name:
SWAP-70
Gene namesi
Name:Swap70
Synonyms:Kiaa0640
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1298390 Swap70

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002402811 – 585Switch-associated protein 70Add BLAST585

Post-translational modificationi

Tyrosine-phosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ6A028
MaxQBiQ6A028
PaxDbiQ6A028
PeptideAtlasiQ6A028
PRIDEiQ6A028

PTM databases

iPTMnetiQ6A028
PhosphoSitePlusiQ6A028

Expressioni

Tissue specificityi

Spleen. Expressed only in B-cells that have been induced to switch to various Ig isotypes.2 Publications

Developmental stagei

Not detected in the spleen of 1-week old mice. Detected from 2-weeks onwards and thereafter levels increase and then from 12-weeks onwards levels decline.1 Publication

Gene expression databases

BgeeiENSMUSG00000031015
CleanExiMM_SWAP70
GenevisibleiQ6A028 MM

Interactioni

Subunit structurei

The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Itga7Q61738-62EBI-2121215,EBI-1786329

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203593, 1 interactor
CORUMiQ6A028
IntActiQ6A028, 2 interactors
MINTiQ6A028
STRINGi10090.ENSMUSP00000033325

Structurei

3D structure databases

ProteinModelPortaliQ6A028
SMRiQ6A028
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini210 – 306PHPROSITE-ProRule annotationAdd BLAST97

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili316 – 529Sequence analysisAdd BLAST214

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi291 – 294Poly-Lys4

Domaini

The PH domain is essential for phosphatidylinositol 3,4,5-trisphosphate binding.By similarity

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IIJW Eukaryota
ENOG410XRWV LUCA
GeneTreeiENSGT00390000005512
HOGENOMiHOG000285974
HOVERGENiHBG053201
InParanoidiQ6A028
KOiK20072
OMAiIKCFDKT
OrthoDBiEOG091G0SJG
PhylomeDBiQ6A028
TreeFamiTF333160

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
PfamiView protein in Pfam
PF00169 PH, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SUPFAMiSSF47473 SSF47473, 2 hits
PROSITEiView protein in PROSITE
PS50003 PH_DOMAIN, 1 hit

Sequencei

Sequence statusi: Complete.

Q6A028-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGLKDELLK AIWHAFTALD LDRSGKVSKS QLKVLSHNLC TVLKVPHDPV
60 70 80 90 100
ALEEHFRDDD EGPVSNQGYM PYLNKFILEK VQDNFDKIEF NRMCWTLCVK
110 120 130 140 150
KNLTKSPLLI TEDDAFKVWV IFNFLSEDKY PLIIVPEEIE YLLKKLTEAM
160 170 180 190 200
GGGWQQEQFE HYKINFDDNK DGLSAWELIE LIGNGQFSKG MDRQTVSMAI
210 220 230 240 250
NEVFNELILD VLKQGYMMKK GHKRKNWTER WFVLKPNIIS YYVSEDLKDK
260 270 280 290 300
KGDILLDENC CVESLPDKDG KKCLFLIKCF DKTFEISASD KKKKQEWIQA
310 320 330 340 350
IYSTIHLLKL GSPPPHKEAR QRRKELRRKL LAEQEELERQ MKELQAANEN
360 370 380 390 400
KQQELESVRK KLEEAASRAA DEEKKRLQTQ VELQTRFSTE LEREKLIRQQ
410 420 430 440 450
MEEQVAQKSS ELEQYLQRVR ELEDMYLKLQ EALEDERQAR QDEETVRKLQ
460 470 480 490 500
ARLLEEESSK RAELEKWHLE QQQAIQTTEA EKQELEQQRV MKEQALQEAM
510 520 530 540 550
AQLEQLELER KQALEQYEGV KKKLEMATHM TKSWKDKVAH HEGLIRLIEP
560 570 580
GSKNPHLITN WGPAAFTQAE LEEREKSWKE KKTTE
Length:585
Mass (Da):68,996
Last modified:June 13, 2006 - v2
Checksum:i3FFD1B671976782C
GO

Sequence cautioni

The sequence BAD32268 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2R → G in BAD32268 (PubMed:15368895).Curated1
Sequence conflicti179I → V in BAD32268 (PubMed:15368895).Curated1
Sequence conflicti543G → R in AAH65136 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053974 mRNA Translation: AAC40155.1
AK172990 mRNA Translation: BAD32268.1 Different initiation.
AK146773 mRNA Translation: BAE27422.1
AK150621 mRNA Translation: BAE29711.1
AK163357 mRNA Translation: BAE37316.1
BC065136 mRNA Translation: AAH65136.1
CCDSiCCDS21744.1
RefSeqiNP_033328.3, NM_009302.3
UniGeneiMm.334144

Genome annotation databases

EnsembliENSMUST00000033325; ENSMUSP00000033325; ENSMUSG00000031015
GeneIDi20947
KEGGimmu:20947
UCSCiuc009jfb.2 mouse

Similar proteinsi

Entry informationi

Entry nameiSWP70_MOUSE
AccessioniPrimary (citable) accession number: Q6A028
Secondary accession number(s): O88443
, Q3TQR6, Q3UCA3, Q6P1D0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 13, 2006
Last modified: March 28, 2018
This is version 119 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health