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Protein

EH domain-binding protein 1

Gene

Ehbp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May play a role in actin reorganization. Links clathrin-mediated endocytosis to the actin cytoskeleton. May act as Rab effector protein and play a role in vesicle trafficking (By similarity). Required for perinuclear sorting and insulin-regulated recycling of SLC2A4/GLUT4 in adipocytes.By similarity1 Publication

GO - Biological processi

Keywordsi

Biological processEndocytosis, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
EH domain-binding protein 1
Gene namesi
Name:Ehbp1
Synonyms:Kiaa0903
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2667252 Ehbp1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002852031 – 1231EH domain-binding protein 1Add BLAST1231

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei171PhosphoserineCombined sources1
Modified residuei174PhosphoserineCombined sources1
Modified residuei177PhosphoserineBy similarity1
Modified residuei222PhosphoserineCombined sources1
Modified residuei302PhosphoserineBy similarity1
Modified residuei307PhosphoserineBy similarity1
Modified residuei328PhosphothreonineCombined sources1
Modified residuei335PhosphoserineCombined sources1
Modified residuei436PhosphoserineCombined sources1
Modified residuei438PhosphoserineBy similarity1
Modified residuei442PhosphoserineCombined sources1
Modified residuei446PhosphoserineCombined sources1
Modified residuei646PhosphoserineCombined sources1
Modified residuei719PhosphoserineBy similarity1
Modified residuei765PhosphoserineCombined sources1
Modified residuei784PhosphoserineBy similarity1
Modified residuei1061PhosphoserineBy similarity1

Post-translational modificationi

Prenylated (Probable). Farnelysation (predominant) and geranylgeranylation has been observed in vitro.By similarity

Keywords - PTMi

Lipoprotein, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiQ69ZW3
PeptideAtlasiQ69ZW3
PRIDEiQ69ZW3

PTM databases

iPTMnetiQ69ZW3
PhosphoSitePlusiQ69ZW3

Expressioni

Gene expression databases

BgeeiENSMUSG00000042302
CleanExiMM_EHBP1
ExpressionAtlasiQ69ZW3 baseline and differential
GenevisibleiQ69ZW3 MM

Interactioni

Subunit structurei

Interacts with EHD1 (PubMed:15247266). Interacts with EHD2. Interacts with RAB8A, RAB10, RAB13 and RAB15 (in their GTP-bound forms); at least in case of RAB8A may bind 2 molecules of RAB8A simultaneously through a high and a low affinity binding site, respectively (By similarity).By similarity1 Publication

Protein-protein interaction databases

BioGridi229762, 2 interactors
STRINGi10090.ENSMUSP00000105191

Structurei

3D structure databases

ProteinModelPortaliQ69ZW3
SMRiQ69ZW3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 158C2 NT-typePROSITE-ProRule annotationAdd BLAST151
Domaini453 – 558Calponin-homology (CH)PROSITE-ProRule annotationAdd BLAST106
Domaini1059 – 1212bMERBPROSITE-ProRule annotationAdd BLAST154

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili185 – 219Sequence analysisAdd BLAST35
Coiled coili812 – 882Sequence analysisAdd BLAST71
Coiled coili1032 – 1100Sequence analysisAdd BLAST69
Coiled coili1136 – 1230Sequence analysisAdd BLAST95

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1228 – 1231CAAX motifBy similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi400 – 447Pro-richAdd BLAST48
Compositional biasi1110 – 1113Poly-Glu4

Domaini

The CAAX motif is a signal for prenylation and required for endosomal colocalization with Rab8 and Rab10.By similarity
The bivalent Mical/EHBP Rab binding (bMERB) domain, mediates binding to Rab8, Rab10, Rab10, Rab13 and Rab15 (in their GTP-bound form).By similarity

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0035 Eukaryota
COG5069 LUCA
GeneTreeiENSGT00760000118856
HOGENOMiHOG000230760
HOVERGENiHBG057909
InParanoidiQ69ZW3
OMAiDDPGICS
OrthoDBiEOG091G01M4
TreeFamiTF105382

Family and domain databases

CDDicd00014 CH, 1 hit
Gene3Di1.10.418.10, 1 hit
InterProiView protein in InterPro
IPR022735 bMERB_dom
IPR001715 CH-domain
IPR036872 CH_dom_sf
IPR029944 EHBP1
IPR019448 NT-C2
PANTHERiPTHR44558 PTHR44558, 1 hit
PfamiView protein in Pfam
PF00307 CH, 1 hit
PF12130 DUF3585, 1 hit
PF10358 NT-C2, 1 hit
SMARTiView protein in SMART
SM00033 CH, 1 hit
SUPFAMiSSF47576 SSF47576, 1 hit
PROSITEiView protein in PROSITE
PS51848 BMERB, 1 hit
PS51840 C2_NT, 1 hit
PS50021 CH, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q69ZW3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASVWKRLQR VGKHASKFQF VASYQELMVE CTKKWQPDKL VVVWTRRSRR
60 70 80 90 100
KSSKAHSWQP GIKNPYRGVV VWPVPENIEI TVTLFKDPHA EEFEDKEWTF
110 120 130 140 150
VIENESPSGR RKALATSSIN MKQYASPMPT QTDVKLKFKP LSKKVVSATL
160 170 180 190 200
QFSLSCIFLR EGKATDEDMQ SLASLMSMKQ ADIGNLDDFE EDNEDDDENR
210 220 230 240 250
VNQEEKAAKI TEIVNQLNAL SSLDEDQDDC IKQANVPSAK SASSSEELIN
260 270 280 290 300
TLNFLDEAQK DLATVNTNPF DEPDVTELNP FGDPDSEEPI TETTSPKKPE
310 320 330 340 350
ESFYNNSCNP FKGVQTPQYL NPFDEPETFV MIKDSPPQST RRKNLRPVDM
360 370 380 390 400
SKYLYADSSK SEEELDESNP FYEPKPTSPN NLVNTVQEGE TERRVKRRAP
410 420 430 440 450
APPAPLAPPA PPAPPALTPK TGVNENTVVS AGKDLSTSPK PSPIPSPVLG
460 470 480 490 500
QKPNASQSLL AWCREVTKNY RGVKITNFTT SWRNGLSFCA ILHHFRPDLI
510 520 530 540 550
DYKSLNPQDI KENNKKAYDG FASIGISRLL EPSDMVLLAI PDKLTVMTYL
560 570 580 590 600
YQIRAHFSGQ ELNVVQIEEN SSKSTYKVGN YETDTNSSVD QEKFYAELSD
610 620 630 640 650
LKREPEPHQP ARGAVDLLSQ DDSVFVTDSG VGESESEHQT PDDHLSPSTA
660 670 680 690 700
SPYYRRTKSD TEPQKSQQSS ARTSGSDDPG LSSSTDSAQA LASLGKKRLK
710 720 730 740 750
AENLELSDLC VSDKKKDVSP LSAYEQKLQT VHASSDMEQG KMEKSRSLEC
760 770 780 790 800
RLDGELAITK PNVSSPSKLG YNRDTDFTKK PCASLRQIES DPDADKSTLN
810 820 830 840 850
HADHPNKAVQ HRMLSRQEEL KERARVLLEQ ARRDAAFKVG SKHGGSAAPA
860 870 880 890 900
LCSRQLNDQQ DEERRRQLRE RARQLIAEAR CGVKMSELPS YGEMAAEKLK
910 920 930 940 950
ERSKASGDEN DNIEIDTNEE IPEGFVVGGG DELTNIESDL DNPEQNSKVV
960 970 980 990 1000
DLRLKKLLEA QPQVANLLPS AAQKAVTEAS EQGEKSGVED LRTERLQKAT
1010 1020 1030 1040 1050
ERFRNPVVFN KDSTVRKTQL QSFSQYVENR PEMKRQRSIQ EDTKRGTEEK
1060 1070 1080 1090 1100
AEITETQRKP SEDEKGFKDT SQYVVGELAA LENEQKQIDT RAALVEKRLR
1110 1120 1130 1140 1150
YLMDTGRNTE EEEAMMQEWF MLVNKKNALI RRMNQLSLLE KEHDLERRYE
1160 1170 1180 1190 1200
LLNRELRAML AIEDWQKTEA QKRREQLLLD ELVALVDKRD ALVRDLDAQE
1210 1220 1230
KQAEEEDEHL ERTLEQNKGK MAKKEEKCAL Q
Length:1,231
Mass (Da):139,104
Last modified:July 27, 2011 - v3
Checksum:iE23307E35621A7A2
GO
Isoform 2 (identifier: Q69ZW3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     288-312: Missing.

Note: No experimental confirmation available.
Show »
Length:1,206
Mass (Da):136,234
Checksum:iD49618A5DD5D2A82
GO

Sequence cautioni

The sequence BAD32333 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti46 – 47RR → KE in AAL24806 (PubMed:12174196).Curated2
Sequence conflicti51K → R in AAL24806 (PubMed:12174196).Curated1
Sequence conflicti797S → N in BAD32333 (PubMed:15368895).Curated1
Sequence conflicti993T → A in AAL24806 (PubMed:12174196).Curated1
Sequence conflicti999A → G in AAL24806 (PubMed:12174196).Curated1
Sequence conflicti1089D → G in AAL24806 (PubMed:12174196).Curated1
Sequence conflicti1155E → A in AAL24806 (PubMed:12174196).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_024836288 – 312Missing in isoform 2. 1 PublicationAdd BLAST25

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF424697 mRNA Translation: AAL24806.1
AK173055 mRNA Translation: BAD32333.1 Different initiation.
AL731860, AL669858 Genomic DNA Translation: CAI25363.1
AL669858, AL731860 Genomic DNA Translation: CAI25873.1
CCDSiCCDS24469.1 [Q69ZW3-2]
CCDS56762.1 [Q69ZW3-1]
RefSeqiNP_001239444.1, NM_001252515.1 [Q69ZW3-1]
NP_694718.4, NM_153078.4 [Q69ZW3-2]
UniGeneiMm.491265

Genome annotation databases

EnsembliENSMUST00000045167; ENSMUSP00000037489; ENSMUSG00000042302 [Q69ZW3-2]
ENSMUST00000109563; ENSMUSP00000105191; ENSMUSG00000042302 [Q69ZW3-1]
ENSMUST00000180360; ENSMUSP00000136697; ENSMUSG00000042302 [Q69ZW3-2]
GeneIDi216565
KEGGimmu:216565
UCSCiuc007iea.2 mouse [Q69ZW3-2]
uc007ieb.2 mouse [Q69ZW3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiEHBP1_MOUSE
AccessioniPrimary (citable) accession number: Q69ZW3
Secondary accession number(s): E9QMJ9, Q5SQK3, Q91ZJ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 106 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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