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Protein

EH domain-binding protein 1

Gene

Ehbp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in actin reorganization. Links clathrin-mediated endocytosis to the actin cytoskeleton (By similarity).By similarity

Names & Taxonomyi

Protein namesi
Recommended name:
EH domain-binding protein 1
Gene namesi
Name:Ehbp1
Synonyms:Kiaa0903
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2667252. Ehbp1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12311231EH domain-binding protein 1PRO_0000285203Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei171 – 1711PhosphoserineCombined sources
Modified residuei174 – 1741PhosphoserineCombined sources
Modified residuei177 – 1771PhosphoserineBy similarity
Modified residuei222 – 2221PhosphoserineCombined sources
Modified residuei328 – 3281PhosphothreonineCombined sources
Modified residuei335 – 3351PhosphoserineCombined sources
Modified residuei436 – 4361PhosphoserineCombined sources
Modified residuei442 – 4421PhosphoserineCombined sources
Modified residuei446 – 4461PhosphoserineCombined sources
Modified residuei646 – 6461PhosphoserineCombined sources
Modified residuei719 – 7191PhosphoserineBy similarity
Modified residuei765 – 7651PhosphoserineCombined sources
Modified residuei1061 – 10611PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ69ZW3.
PaxDbiQ69ZW3.
PRIDEiQ69ZW3.

PTM databases

iPTMnetiQ69ZW3.
PhosphoSiteiQ69ZW3.

Expressioni

Gene expression databases

BgeeiQ69ZW3.
CleanExiMM_EHBP1.
ExpressionAtlasiQ69ZW3. baseline and differential.
GenevisibleiQ69ZW3. MM.

Interactioni

Subunit structurei

Interacts with EHD2.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000105191.

Structurei

3D structure databases

ProteinModelPortaliQ69ZW3.
SMRiQ69ZW3. Positions 453-558.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 163151NT-C2Sequence analysisAdd
BLAST
Domaini453 – 555103CHPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili185 – 21935Sequence analysisAdd
BLAST
Coiled coili812 – 88271Sequence analysisAdd
BLAST
Coiled coili1032 – 110069Sequence analysisAdd
BLAST
Coiled coili1136 – 123095Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi400 – 44748Pro-richAdd
BLAST
Compositional biasi1110 – 11134Poly-Glu

Sequence similaritiesi

Contains 1 CH (calponin-homology) domain.PROSITE-ProRule annotation
Contains 1 NT-C2 domain.Sequence analysis

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0035. Eukaryota.
COG5069. LUCA.
GeneTreeiENSGT00760000118856.
HOGENOMiHOG000230760.
HOVERGENiHBG057909.
InParanoidiQ69ZW3.
OMAiKNVRPVD.
OrthoDBiEOG7ZKS97.
TreeFamiTF105382.

Family and domain databases

Gene3Di1.10.418.10. 1 hit.
InterProiIPR001715. CH-domain.
IPR022735. DUF3585.
IPR029944. EHBP1.
IPR019448. NT-C2.
[Graphical view]
PANTHERiPTHR11915:SF289. PTHR11915:SF289. 1 hit.
PfamiPF00307. CH. 1 hit.
PF12130. DUF3585. 1 hit.
PF10358. NT-C2. 1 hit.
[Graphical view]
SMARTiSM00033. CH. 1 hit.
[Graphical view]
SUPFAMiSSF47576. SSF47576. 1 hit.
PROSITEiPS50021. CH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q69ZW3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASVWKRLQR VGKHASKFQF VASYQELMVE CTKKWQPDKL VVVWTRRSRR
60 70 80 90 100
KSSKAHSWQP GIKNPYRGVV VWPVPENIEI TVTLFKDPHA EEFEDKEWTF
110 120 130 140 150
VIENESPSGR RKALATSSIN MKQYASPMPT QTDVKLKFKP LSKKVVSATL
160 170 180 190 200
QFSLSCIFLR EGKATDEDMQ SLASLMSMKQ ADIGNLDDFE EDNEDDDENR
210 220 230 240 250
VNQEEKAAKI TEIVNQLNAL SSLDEDQDDC IKQANVPSAK SASSSEELIN
260 270 280 290 300
TLNFLDEAQK DLATVNTNPF DEPDVTELNP FGDPDSEEPI TETTSPKKPE
310 320 330 340 350
ESFYNNSCNP FKGVQTPQYL NPFDEPETFV MIKDSPPQST RRKNLRPVDM
360 370 380 390 400
SKYLYADSSK SEEELDESNP FYEPKPTSPN NLVNTVQEGE TERRVKRRAP
410 420 430 440 450
APPAPLAPPA PPAPPALTPK TGVNENTVVS AGKDLSTSPK PSPIPSPVLG
460 470 480 490 500
QKPNASQSLL AWCREVTKNY RGVKITNFTT SWRNGLSFCA ILHHFRPDLI
510 520 530 540 550
DYKSLNPQDI KENNKKAYDG FASIGISRLL EPSDMVLLAI PDKLTVMTYL
560 570 580 590 600
YQIRAHFSGQ ELNVVQIEEN SSKSTYKVGN YETDTNSSVD QEKFYAELSD
610 620 630 640 650
LKREPEPHQP ARGAVDLLSQ DDSVFVTDSG VGESESEHQT PDDHLSPSTA
660 670 680 690 700
SPYYRRTKSD TEPQKSQQSS ARTSGSDDPG LSSSTDSAQA LASLGKKRLK
710 720 730 740 750
AENLELSDLC VSDKKKDVSP LSAYEQKLQT VHASSDMEQG KMEKSRSLEC
760 770 780 790 800
RLDGELAITK PNVSSPSKLG YNRDTDFTKK PCASLRQIES DPDADKSTLN
810 820 830 840 850
HADHPNKAVQ HRMLSRQEEL KERARVLLEQ ARRDAAFKVG SKHGGSAAPA
860 870 880 890 900
LCSRQLNDQQ DEERRRQLRE RARQLIAEAR CGVKMSELPS YGEMAAEKLK
910 920 930 940 950
ERSKASGDEN DNIEIDTNEE IPEGFVVGGG DELTNIESDL DNPEQNSKVV
960 970 980 990 1000
DLRLKKLLEA QPQVANLLPS AAQKAVTEAS EQGEKSGVED LRTERLQKAT
1010 1020 1030 1040 1050
ERFRNPVVFN KDSTVRKTQL QSFSQYVENR PEMKRQRSIQ EDTKRGTEEK
1060 1070 1080 1090 1100
AEITETQRKP SEDEKGFKDT SQYVVGELAA LENEQKQIDT RAALVEKRLR
1110 1120 1130 1140 1150
YLMDTGRNTE EEEAMMQEWF MLVNKKNALI RRMNQLSLLE KEHDLERRYE
1160 1170 1180 1190 1200
LLNRELRAML AIEDWQKTEA QKRREQLLLD ELVALVDKRD ALVRDLDAQE
1210 1220 1230
KQAEEEDEHL ERTLEQNKGK MAKKEEKCAL Q
Length:1,231
Mass (Da):139,104
Last modified:July 27, 2011 - v3
Checksum:iE23307E35621A7A2
GO
Isoform 2 (identifier: Q69ZW3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     288-312: Missing.

Note: No experimental confirmation available.
Show »
Length:1,206
Mass (Da):136,234
Checksum:iD49618A5DD5D2A82
GO

Sequence cautioni

The sequence BAD32333.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti46 – 472RR → KE in AAL24806 (PubMed:12174196).Curated
Sequence conflicti51 – 511K → R in AAL24806 (PubMed:12174196).Curated
Sequence conflicti797 – 7971S → N in BAD32333 (PubMed:15368895).Curated
Sequence conflicti993 – 9931T → A in AAL24806 (PubMed:12174196).Curated
Sequence conflicti999 – 9991A → G in AAL24806 (PubMed:12174196).Curated
Sequence conflicti1089 – 10891D → G in AAL24806 (PubMed:12174196).Curated
Sequence conflicti1155 – 11551E → A in AAL24806 (PubMed:12174196).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei288 – 31225Missing in isoform 2. 1 PublicationVSP_024836Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF424697 mRNA. Translation: AAL24806.1.
AK173055 mRNA. Translation: BAD32333.1. Different initiation.
AL731860, AL669858 Genomic DNA. Translation: CAI25363.1.
AL669858, AL731860 Genomic DNA. Translation: CAI25873.1.
CCDSiCCDS24469.1. [Q69ZW3-2]
CCDS56762.1. [Q69ZW3-1]
RefSeqiNP_001239444.1. NM_001252515.1. [Q69ZW3-1]
NP_694718.4. NM_153078.4. [Q69ZW3-2]
UniGeneiMm.491265.

Genome annotation databases

EnsembliENSMUST00000045167; ENSMUSP00000037489; ENSMUSG00000042302. [Q69ZW3-2]
ENSMUST00000109563; ENSMUSP00000105191; ENSMUSG00000042302. [Q69ZW3-1]
ENSMUST00000180360; ENSMUSP00000136697; ENSMUSG00000042302. [Q69ZW3-2]
GeneIDi216565.
KEGGimmu:216565.
UCSCiuc007iea.2. mouse. [Q69ZW3-2]
uc007ieb.2. mouse. [Q69ZW3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF424697 mRNA. Translation: AAL24806.1.
AK173055 mRNA. Translation: BAD32333.1. Different initiation.
AL731860, AL669858 Genomic DNA. Translation: CAI25363.1.
AL669858, AL731860 Genomic DNA. Translation: CAI25873.1.
CCDSiCCDS24469.1. [Q69ZW3-2]
CCDS56762.1. [Q69ZW3-1]
RefSeqiNP_001239444.1. NM_001252515.1. [Q69ZW3-1]
NP_694718.4. NM_153078.4. [Q69ZW3-2]
UniGeneiMm.491265.

3D structure databases

ProteinModelPortaliQ69ZW3.
SMRiQ69ZW3. Positions 453-558.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000105191.

PTM databases

iPTMnetiQ69ZW3.
PhosphoSiteiQ69ZW3.

Proteomic databases

MaxQBiQ69ZW3.
PaxDbiQ69ZW3.
PRIDEiQ69ZW3.

Protocols and materials databases

DNASUi216565.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045167; ENSMUSP00000037489; ENSMUSG00000042302. [Q69ZW3-2]
ENSMUST00000109563; ENSMUSP00000105191; ENSMUSG00000042302. [Q69ZW3-1]
ENSMUST00000180360; ENSMUSP00000136697; ENSMUSG00000042302. [Q69ZW3-2]
GeneIDi216565.
KEGGimmu:216565.
UCSCiuc007iea.2. mouse. [Q69ZW3-2]
uc007ieb.2. mouse. [Q69ZW3-1]

Organism-specific databases

CTDi23301.
MGIiMGI:2667252. Ehbp1.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0035. Eukaryota.
COG5069. LUCA.
GeneTreeiENSGT00760000118856.
HOGENOMiHOG000230760.
HOVERGENiHBG057909.
InParanoidiQ69ZW3.
OMAiKNVRPVD.
OrthoDBiEOG7ZKS97.
TreeFamiTF105382.

Miscellaneous databases

ChiTaRSiEhbp1. mouse.
PROiQ69ZW3.
SOURCEiSearch...

Gene expression databases

BgeeiQ69ZW3.
CleanExiMM_EHBP1.
ExpressionAtlasiQ69ZW3. baseline and differential.
GenevisibleiQ69ZW3. MM.

Family and domain databases

Gene3Di1.10.418.10. 1 hit.
InterProiIPR001715. CH-domain.
IPR022735. DUF3585.
IPR029944. EHBP1.
IPR019448. NT-C2.
[Graphical view]
PANTHERiPTHR11915:SF289. PTHR11915:SF289. 1 hit.
PfamiPF00307. CH. 1 hit.
PF12130. DUF3585. 1 hit.
PF10358. NT-C2. 1 hit.
[Graphical view]
SMARTiSM00033. CH. 1 hit.
[Graphical view]
SUPFAMiSSF47576. SSF47576. 1 hit.
PROSITEiPS50021. CH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Comparative transcription map of the wobbler critical region on mouse chromosome 11 and the homologous region on human chromosome 2p13-14."
    Fuchs S., Resch K., Thiel C., Ulbrich M., Platzer M., Jockusch H., Schmitt-John T.
    BMC Genet. 3:14-14(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
    DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Fetal brain.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-171; SER-174; SER-222; THR-328; SER-335; SER-436; SER-442; SER-446; SER-646 AND SER-765, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Heart, Kidney, Liver, Lung and Testis.

Entry informationi

Entry nameiEHBP1_MOUSE
AccessioniPrimary (citable) accession number: Q69ZW3
Secondary accession number(s): E9QMJ9, Q5SQK3, Q91ZJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: July 27, 2011
Last modified: June 8, 2016
This is version 90 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.