Q69ZQ2 (ISY1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pre-mRNA-splicing factor ISY1 homolog

Gene names

Name:	Isy1
Synonyms:	Kiaa1160

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in pre-mRNA splicing Potential.
Subunit structure	Identified in the spliceosome C complex By similarity.
Subcellular location	Nucleus Potential.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR By similarity.
Sequence similarities	Belongs to the ISY1 family.
Sequence caution	The sequence BAD32394.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	RNA splicing Inferred from electronic annotation. Source: UniProtKB-KW mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 285

285

Pre-mRNA-splicing factor ISY1 homolog

PRO_0000235814

Regions

Compositional bias

201 – 211

11

Poly-Glu

Amino acid modifications

Modified residue

127

1

N6-acetyllysine By similarity

Modified residue

222

1

Phosphoserine By similarity

Modified residue

247

1

Phosphoserine By similarity

Experimental info

Sequence conflict

133

1

R → K in BAB31028. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

285

Helix Strand Turn

Details...

Turn

33 – 35

3

Helix

39 – 62

24

Helix

67 – 94

28

Sequences

	Sequence		Length	Mass (Da)
	Q69ZQ2 [UniParc]. Last modified May 16, 2006. Version 2. Checksum: 4A456045660F29D6	FASTA	285	32,989
	10 20 30 40 50 60 MARNAEKAMT ALARFRQAQL EEGKVKERRP FLASECTELP KAEKWRRQII GEISKKVAQI 70 80 90 100 110 120 QNAGLGEFRI RDLNDEINKL LREKGHWEVR IKELGGPDYG KVGPKMLDHE GKEVPGNRGY 130 140 150 160 170 180 KYFGAAKDLP GVRELFEKEP LPPPRKTRAE LMKAIDFEYY GYLDEDDGVI VPLEQEYEKK 190 200 210 220 230 240 LRAELVEKWK AEREARLARG EKEEEEEEEE EINIYAVTEE ESDEEGNQEK AGEDGQQKFI 250 260 270 280 AHVPVPSQQE IEEALVRRKK MELLQKYASE TLQAQSEEAK RLLGY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H. DNA Res. 11:205-218(2004) [PubMed: 15368895] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Thymus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II and FVB/N. Tissue: Mammary gland.
[4]	"Solution structure of ISY1 domain in hypothetical protein." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 24-102.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK173116 mRNA. Translation: BAD32394.1. Different initiation.
AK017996 mRNA. Translation: BAB31028.1.
BC020103 mRNA. Translation: AAH20103.1.
BC037695 mRNA. Translation: AAH37695.1.

IPI

IPI00469994.

RefSeq

NP_598695.1. NM_133934.4.

UniGene

Mm.241546.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1X4T	NMR	-	A	23-102	[»]

ProteinModelPortal

Q69ZQ2.

SMR

Q69ZQ2. Positions 23-102.

ModBase

Search...

PTM databases

PhosphoSite

Q69ZQ2.

Proteomic databases

PRIDE

Q69ZQ2.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000089497; ENSMUSP00000086923; ENSMUSG00000030056.

GeneID

57905.

KEGG

mmu:57905.

UCSC

uc009cub.1. mouse.

Organism-specific databases

CTD

57461.

MGI

MGI:1923310. Isy1.

Rouge

Search...

Phylogenomic databases

eggNOG

roNOG07206.

HOGENOM

HBG628655.

HOVERGEN

HBG061071.

InParanoid

Q69ZQ2.

OMA

RRYGPKM.

OrthoDB

EOG4DJJX6.

PhylomeDB

Q69ZQ2.

Gene expression databases

ArrayExpress

Q69ZQ2.

Bgee

Q69ZQ2.

CleanEx

MM_ISY1.

Genevestigator

Q69ZQ2.

GermOnline

ENSMUSG00000030056. Mus musculus.

Family and domain databases

InterPro

IPR009360. Isy1.
[Graphical view]

KO

K12870.

PANTHER

PTHR13021. Isy1. 1 hit.

Pfam

PF06246. Isy1. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

314069.

SOURCE

Search...

Entry information

Entry name

ISY1_MOUSE

Accession

Primary (citable) accession number: Q69ZQ2
Secondary accession number(s): Q8VDX4, Q9D3E2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	May 16, 2006
Last modified:	November 16, 2011
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program