Reviewed, UniProtKB/Swiss-Prot Q69ZM6 (STK36_MOUSE)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase 36
    EC=2.7.11.1
Alternative name(s):
    Fused homolog

Gene names

Name:	Stk36
Synonyms:	Kiaa1278

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	1268 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Serine/threonine protein kinase required for postnatal development, possibly by regulating the homeostasis of cerebral spinal fluid or ciliary function. Controls the activity of the transcriptional regulators GLI1, GLI2 and GLI3 by opposing the effect of SUFU and promoting their nuclear localization. GLI2 requires an additional function of STK36 to become transcriptionally active, but the enzyme does not need to possess an active kinase catalytic site for this to occur By similarity. Ref.4
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. UniProtKB P23647
Cofactor	Magnesium.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Note: Low levels also present in the nucleus By similarity.
Tissue specificity	Weakly expressed in the heart and thymus, present at moderate to high levels in the lungs, pancreas, and kidneys and at higher levels in the brain and cerebellum. Very highly expressed in the testis. Ref.4
Developmental stage	At E13.5 is widely distributed in the forebrain, midbrain, hindbrain, spinal cord, somites, developing limb buds and skin. Ref.3
Disruption phenotype	Mice display profound growth retardation with a communicating form of hydrocephalus, nasal inflammation and early mortality. Ref.4
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.
Sequence caution	The sequence BAD32420.1 differs from that shown. Reason: Frameshift at position 1174.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Developmental protein Kinase Serine/threonine-protein kinase Transferase
Gene Ontology (GO)
Biological process	post-embryonic development Ref.4 Inferred from mutant phenotype. Source: UniProtKB protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription factor activity Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW transcription factor binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 (identifier: Q69ZM6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 Ref.2 (identifier: Q69ZM6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     225-225: V → SCFKNFLQGLLTKDPRQRLSWPDLLHHPFIAGRVTI
     426-553: Missing.
     827-827: Q → QVQ
     984-984: V → VCCHHLSLLQAE

Note: No experimental confirmation available.

Isoform 3 Ref.2 (identifier: Q69ZM6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     225-225: V → SCFKNFLQGLLTKDPRQRLSWPDLLHHPFIAGRVTI
     984-984: V → VCCHHLSLLQAE
     1002-1007: KQFVNT → SGRSLP
     1008-1268: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1268	1268	Serine/threonine-protein kinase 36		PRO_0000229021
Regions
	Domain	4 – 254	251	Protein kinase
	Nucleotide binding	10 – 18	9	ATP By similarity UniProtKB P23647
Sites
	Active site	125	1	Proton acceptor By similarity UniProtKB P23647
	Binding site	33	1	ATP By similarity UniProtKB P23647
Natural variations
	Alternative sequence	225	1	V → SCFKNFLQGLLTKDPRQRLS WPDLLHHPFIAGRVTI in isoform 2 and isoform 3. Ref.2		VSP_051984
	Alternative sequence	426 – 553	128	Missing in isoform 2. Ref.2		VSP_051985
	Alternative sequence	827	1	Q → QVQ in isoform 2. Ref.2		VSP_051986
	Alternative sequence	984	1	V → VCCHHLSLLQAE in isoform 2 and isoform 3. Ref.2		VSP_051987
	Alternative sequence	1002 – 1007	6	KQFVNT → SGRSLP in isoform 3. Ref.2		VSP_051988
	Alternative sequence	1008 – 1268	261	Missing in isoform 3. Ref.2		VSP_051989
Experimental info
	Sequence conflict	295	1	G → R in BAD32420. Ref.1
	Sequence conflict	436	1	Y → H in AAH43103. Ref.2
	Sequence conflict	809	1	Y → C in BAD32420. Ref.1
	Sequence conflict	837	1	V → A in BAD32420. Ref.1
	Sequence conflict	844	1	I → T in BAD32420. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 21, 2006. Version 2. Checksum: 61D0519180A474E8	FASTA	1,268	138,655
	10 20 30 40 50 60 MEKYHVLEMI GEGSFGRVYK GRKKYSAQVV ALKFIPKLGR SEKELRNLQR EIEIMRGLWH 70 80 90 100 110 120 PNIVHMLDSF ETDKEVVVVT DYAEGELFQI LEDDGKLPED QVQAIAAQLV SALYYLHSHR 130 140 150 160 170 180 ILHRDMKPQN ILLAKGGGIK LCDFGFARAM STNTMVLTSI KGTPLYMSPE LVEERPYDHT 190 200 210 220 230 240 ADLWSVGCIL YELAVGTPPF YTTSIFQLVS LILKDPVRWP STISVITEPA GSDLGTPFTS 250 260 270 280 290 300 RLPPELQVLK DEQAHRLAPK GNQSRILRQA CKLMAEEAKQ KEDQNAGSAL EQEDGLCKVT 310 320 330 340 350 360 PSTAPVPGLK ATPQESSLLA GILASEMKNN WEDWGAGEAP RTSRENHINL ECEQGFPEPR 370 380 390 400 410 420 PEAMGRQSTD VVDPENEEPD SDDEWQRLLE TSEPGPVQLK SPLTLLCNPD FCQRIQSQLR 430 440 450 460 470 480 GTGEQILKGV LDGVSYLLPV LRILSSLLSS CNDSVLLYSF CQEAGLPELP LSLLRYSQES 490 500 510 520 530 540 SSIQQQPWYG ALLRDLVAVV QAYFSCTFNL ERSQTGDSLQ VFQEAASLFL DLLGKLLAQS 550 560 570 580 590 600 DDSEQTFRRD SLMCFAVLCE AVDGNSWAVS KAFYSSLLTT QRAVLDGLLH GLTVPQLPFH 610 620 630 640 650 660 TPPGAPQVSQ PLREQSEDVP GAISSALAAM CTAPVGLPSC WDAKEQVSWH LANQLTEDSS 670 680 690 700 710 720 QLRPSLISGL RHHVLCLHLL KVLYACCYIS ERLCHILGQE PLALESLLML VQGKVKVADW 730 740 750 760 770 780 EESTEVALYL LSLLVFRLQD LPSGMEKLGS EVATLFTHSH VVSLVNAAAC LLGQLGQQGV 790 800 810 820 830 840 TFDLQPREWI AAAAHALSAP AEVRLTPPYS CGFYDGLLIL LLQLLMQGKP GLIRDVVGSE 850 860 870 880 890 900 VWTILWHRFS MALRLPEEVS AQEDDLLLSS PSSLEPDWTL ISPQGMAALL SLAMAIFTQE 910 920 930 940 950 960 SQLCLSHLSQ HGSVLMLTLK HLLSPSFLHH LSQAPQGPEF LPVVVLSVCK LLCFPFALDV 970 980 990 1000 1010 1020 DADLLVGVLA DLRASEVVVC LLQVLPIGLL TRLALTDSAS LKQFVNTVAT SSRAIISFLS 1030 1040 1050 1060 1070 1080 VVLLSDQPLM ISDLLSLLTH TARILSPSHL SFIQELLSGS DESYRPLRSL LGHSENTVRV 1090 1100 1110 1120 1130 1140 RAYGLLGHLL QHSMALRGAL QSQSGLLNLL LLGLGDKDPA VRRSASFAVG NAAYQAGPLG 1150 1160 1170 1180 1190 1200 PALAAAVPSM TQLLGDAQDG IRRNAASALG NLGPEGLGKE LLKCQVPQRL LEMACGDPQP 1210 1220 1230 1240 1250 1260 TVKEAALIAL RSLQQESCIH QVLVSLGASE KLALLSLGNQ LLPNSSNRPA SVRHCRKLIQ LLRPTHST
	Isoform 2. Checksum: BE70EF05C8AF9931	FASTA	1,188	129,980
	10 20 30 40 50 60 MEKYHVLEMI GEGSFGRVYK GRKKYSAQVV ALKFIPKLGR SEKELRNLQR EIEIMRGLWH 70 80 90 100 110 120 PNIVHMLDSF ETDKEVVVVT DYAEGELFQI LEDDGKLPED QVQAIAAQLV SALYYLHSHR 130 140 150 160 170 180 ILHRDMKPQN ILLAKGGGIK LCDFGFARAM STNTMVLTSI KGTPLYMSPE LVEERPYDHT 190 200 210 220 230 240 ADLWSVGCIL YELAVGTPPF YTTSIFQLVS LILKDPVRWP STISSCFKNF LQGLLTKDPR 250 260 270 280 290 300 QRLSWPDLLH HPFIAGRVTI ITEPAGSDLG TPFTSRLPPE LQVLKDEQAH RLAPKGNQSR 310 320 330 340 350 360 ILRQACKLMA EEAKQKEDQN AGSALEQEDG LCKVTPSTAP VPGLKATPQE SSLLAGILAS 370 380 390 400 410 420 EMKNNWEDWG AGEAPRTSRE NHINLECEQG FPEPRPEAMG RQSTDVVDPE NEEPDSDDEW 430 440 450 460 470 480 QRLLETSEPG PVQLKSPLTL LCNPDFCQRI QSQLRGTGEQ CFAVLCEAVD GNSWAVSKAF 490 500 510 520 530 540 YSSLLTTQRA VLDGLLHGLT VPQLPFHTPP GAPQVSQPLR EQSEDVPGAI SSALAAMCTA 550 560 570 580 590 600 PVGLPSCWDA KEQVSWHLAN QLTEDSSQLR PSLISGLRHH VLCLHLLKVL YACCYISERL 610 620 630 640 650 660 CHILGQEPLA LESLLMLVQG KVKVADWEES TEVALYLLSL LVFRLQDLPS GMEKLGSEVA 670 680 690 700 710 720 TLFTHSHVVS LVNAAACLLG QLGQQGVTFD LQPREWIAAA AHALSAPAEV RLTPPYSCGF 730 740 750 760 770 780 YDGLLILLLQ LLMQVQGKPG LIRDVVGSEV WTILWHRFSM ALRLPEEVSA QEDDLLLSSP 790 800 810 820 830 840 SSLEPDWTLI SPQGMAALLS LAMAIFTQES QLCLSHLSQH GSVLMLTLKH LLSPSFLHHL 850 860 870 880 890 900 SQAPQGPEFL PVVVLSVCKL LCFPFALDVD ADLLVGVLAD LRASEVVVCL LQVCCHHLSL 910 920 930 940 950 960 LQAELPIGLL TRLALTDSAS LKQFVNTVAT SSRAIISFLS VVLLSDQPLM ISDLLSLLTH 970 980 990 1000 1010 1020 TARILSPSHL SFIQELLSGS DESYRPLRSL LGHSENTVRV RAYGLLGHLL QHSMALRGAL 1030 1040 1050 1060 1070 1080 QSQSGLLNLL LLGLGDKDPA VRRSASFAVG NAAYQAGPLG PALAAAVPSM TQLLGDAQDG 1090 1100 1110 1120 1130 1140 IRRNAASALG NLGPEGLGKE LLKCQVPQRL LEMACGDPQP TVKEAALIAL RSLQQESCIH 1150 1160 1170 1180 QVLVSLGASE KLALLSLGNQ LLPNSSNRPA SVRHCRKLIQ LLRPTHST
	Isoform 3. Checksum: 0A4E05231C5034FE	FASTA	1,053	116,191
	10 20 30 40 50 60 MEKYHVLEMI GEGSFGRVYK GRKKYSAQVV ALKFIPKLGR SEKELRNLQR EIEIMRGLWH 70 80 90 100 110 120 PNIVHMLDSF ETDKEVVVVT DYAEGELFQI LEDDGKLPED QVQAIAAQLV SALYYLHSHR 130 140 150 160 170 180 ILHRDMKPQN ILLAKGGGIK LCDFGFARAM STNTMVLTSI KGTPLYMSPE LVEERPYDHT 190 200 210 220 230 240 ADLWSVGCIL YELAVGTPPF YTTSIFQLVS LILKDPVRWP STISSCFKNF LQGLLTKDPR 250 260 270 280 290 300 QRLSWPDLLH HPFIAGRVTI ITEPAGSDLG TPFTSRLPPE LQVLKDEQAH RLAPKGNQSR 310 320 330 340 350 360 ILRQACKLMA EEAKQKEDQN AGSALEQEDG LCKVTPSTAP VPGLKATPQE SSLLAGILAS 370 380 390 400 410 420 EMKNNWEDWG AGEAPRTSRE NHINLECEQG FPEPRPEAMG RQSTDVVDPE NEEPDSDDEW 430 440 450 460 470 480 QRLLETSEPG PVQLKSPLTL LCNPDFCQRI QSQLRGTGEQ ILKGVLDGVS YLLPVLRILS 490 500 510 520 530 540 SLLSSCNDSV LLYSFCQEAG LPELPLSLLR YSQESSSIQQ QPWYGALLRD LVAVVQAYFS 550 560 570 580 590 600 CTFNLERSQT GDSLQVFQEA ASLFLDLLGK LLAQSDDSEQ TFRRDSLMCF AVLCEAVDGN 610 620 630 640 650 660 SWAVSKAFYS SLLTTQRAVL DGLLHGLTVP QLPFHTPPGA PQVSQPLREQ SEDVPGAISS 670 680 690 700 710 720 ALAAMCTAPV GLPSCWDAKE QVSWHLANQL TEDSSQLRPS LISGLRHHVL CLHLLKVLYA 730 740 750 760 770 780 CCYISERLCH ILGQEPLALE SLLMLVQGKV KVADWEESTE VALYLLSLLV FRLQDLPSGM 790 800 810 820 830 840 EKLGSEVATL FTHSHVVSLV NAAACLLGQL GQQGVTFDLQ PREWIAAAAH ALSAPAEVRL 850 860 870 880 890 900 TPPYSCGFYD GLLILLLQLL MQGKPGLIRD VVGSEVWTIL WHRFSMALRL PEEVSAQEDD 910 920 930 940 950 960 LLLSSPSSLE PDWTLISPQG MAALLSLAMA IFTQESQLCL SHLSQHGSVL MLTLKHLLSP 970 980 990 1000 1010 1020 SFLHHLSQAP QGPEFLPVVV LSVCKLLCFP FALDVDADLL VGVLADLRAS EVVVCLLQVC 1030 1040 1050 CHHLSLLQAE LPIGLLTRLA LTDSASLSGR SLP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H. DNA Res. 11:205-218(2004) [PubMed: 15368895] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Fetal brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Strain: C57BL/6. Tissue: Brain.
[3]	"Gli regulation by the opposing activities of fused and suppressor of fused." Murone M., Luoh S.-L., Stone D., Li W., Gurney A., Armanini M., Grey C., Rosenthal A., de Sauvage F.J. Nat. Cell Biol. 2:310-312(2000) [PubMed: 10806483] [Abstract] Cited for: DEVELOPMENTAL STAGE.
[4]	"Loss of the serine/threonine kinase fused results in postnatal growth defects and lethality due to progressive hydrocephalus." Merchant M., Evangelista M., Luoh S.-M., Frantz G.D., Chalasani S., Carano R.A., van Hoy M., Ramirez J., Ogasawara A.K., McFarland L.M., Filvaroff E.H., French D.M., de Sauvage F.J. Mol. Cell. Biol. 25:7054-7068(2005) [PubMed: 16055717] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK173142 Transcribed RNA. Translation: BAD32420.1. Sequence problems. BC043103 mRNA. Translation: AAH43103.1. BC058698 mRNA. Translation: AAH58698.1.
IPI	IPI00187463. IPI00461604. IPI00621144.
RefSeq	NP_778196.2.
UniGene	Mm.310974
3D structure databases
SMR	Q69ZM6. Positions 1-207, 1069-1217.
ModBase	Search...
Protein-protein interaction databases
STRING	Q69ZM6.
Proteomic databases
PRIDE	Q69ZM6.
Genome annotation databases
Ensembl	ENSMUST00000042446; ENSMUSP00000040924; ENSMUSG00000033276; Mus musculus. [Genome view] ENSMUST00000087183; ENSMUSP00000084430; ENSMUSG00000033276; Mus musculus. [Genome view] ENSMUST00000113699; ENSMUSP00000109329; ENSMUSG00000033276; Mus musculus. [Genome view]
GeneID	269209.
KEGG	mmu:269209.
UCSC	uc007bmu.1. mouse.
Organism-specific databases
CTD	269209.
MGI	MGI:1920831. Stk36.
Rouge	Search...
Phylogenomic databases
eggNOG	roNOG07048.
HOVERGEN	Q69ZM6.
PhylomeDB	Q69ZM6.
Enzyme and pathway databases
BRENDA	2.7.11.1. 244.
Gene expression databases
ArrayExpress	Q69ZM6.
Bgee	Q69ZM6.
Genevestigator	Q69ZM6.
GermOnline	ENSMUSG00000033276. Mus musculus.
Family and domain databases
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Gene3D	G3DSA:1.25.10.10. ARM-like. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	392738.
SOURCE	Search...

Entry information

Entry name

STK36_MOUSE

Accession

Primary (citable) accession number: Q69ZM6
Secondary accession number(s): Q6PDI0, Q80XQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	March 21, 2006
Last modified:	January 19, 2010
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)