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Protein

Neuroligin-2

Gene

Nlgn2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transmembrane scaffolding protein involved in cell-cell interactions via its interactions with neurexin family members. Mediates cell-cell interactions both in neurons and in other types of cells, such as Langerhans beta cells. Mediates cell-cell interactions between Langerhans beta cells and modulates insulin secretion (By similarity). Plays a role in synapse function and synaptic signal transmission, especially via gamma-aminobutyric acid receptors (GABA(A) receptors). Functions by recruiting and clustering synaptic proteins. Promotes clustering of postsynaptic GABRG2 and GPHN. Modulates signaling by inhibitory synapses, and thereby plays a role in controlling the ratio of signaling by excitatory and inhibitory synapses and information processing. Required for normal signal amplitude from inhibitory synapses, but is not essential for normal signal frequency. May promote the initial formation of synapses, but is not essential for this. In vitro, triggers the de novo formation of presynaptic structures.By similarity6 Publications

GO - Molecular functioni

  • carboxylic ester hydrolase activity Source: GO_Central
  • cell adhesion molecule binding Source: BHF-UCL
  • neurexin family protein binding Source: BHF-UCL
  • receptor activity Source: GO_Central

GO - Biological processi

  • gephyrin clustering involved in postsynaptic density assembly Source: BHF-UCL
  • inhibitory synapse assembly Source: ParkinsonsUK-UCL
  • jump response Source: BHF-UCL
  • locomotory exploration behavior Source: BHF-UCL
  • modulation of synaptic transmission Source: MGI
  • neuromuscular process controlling balance Source: BHF-UCL
  • neuron cell-cell adhesion Source: BHF-UCL
  • positive regulation of excitatory postsynaptic potential Source: BHF-UCL
  • positive regulation of inhibitory postsynaptic potential Source: BHF-UCL
  • positive regulation of insulin secretion Source: MGI
  • positive regulation of protein localization to synapse Source: MGI
  • positive regulation of synapse assembly Source: BHF-UCL
  • positive regulation of synaptic transmission, GABAergic Source: BHF-UCL
  • positive regulation of synaptic transmission, glutamatergic Source: BHF-UCL
  • positive regulation of synaptic vesicle clustering Source: BHF-UCL
  • postsynaptic density protein 95 clustering Source: BHF-UCL
  • postsynaptic membrane assembly Source: BHF-UCL
  • presynaptic membrane assembly Source: BHF-UCL
  • protein localization to synapse Source: BHF-UCL
  • regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity Source: BHF-UCL
  • regulation of respiratory gaseous exchange by neurological system process Source: MGI
  • sensory perception of pain Source: BHF-UCL
  • social behavior Source: BHF-UCL
  • synapse assembly Source: BHF-UCL
  • synapse organization Source: BHF-UCL
  • terminal button organization Source: BHF-UCL
  • thigmotaxis Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiR-MMU-6794361. Interactions of neurexins and neuroligins at synapses.

Protein family/group databases

ESTHERimouse-2neur. Neuroligin.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuroligin-2
Gene namesi
Name:Nlgn2
Synonyms:Kiaa1366
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2681835. Nlgn2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini15 – 678ExtracellularSequence analysisAdd BLAST664
Transmembranei679 – 699HelicalSequence analysisAdd BLAST21
Topological domaini700 – 836CytoplasmicSequence analysisAdd BLAST137

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cell surface Source: BHF-UCL
  • excitatory synapse Source: BHF-UCL
  • inhibitory synapse Source: BHF-UCL
  • integral component of plasma membrane Source: GO_Central
  • integral component of postsynaptic membrane Source: BHF-UCL
  • presynaptic membrane Source: UniProtKB-SubCell
  • synapse Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

No obvious phenotype, but mice present subtle behavorial changes. Signaling from inhibitory synapses is impaired. In addition, mice have reduced brain volume. Mice lacking both NLGN1 and NLGN2, or NLGN2 and NLGN3, are viable, but have impaired breathing, drastically reduced reproduction rates and striking deficits in raising their offspring. Mice lacking NLGN1, NLGN2 and NLGN3 are born at the expected Mendelian rate, but die shortly after birth due to respiratory failure. They do not show a significant change in the number of synapses, but synapse function is strongly impaired.4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 14By similarityAdd BLAST14
ChainiPRO_000004187915 – 836Neuroligin-2Add BLAST822

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi98N-linked (GlcNAc...)1 Publication1
Disulfide bondi106 ↔ 1411 Publication
Glycosylationi136N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi317 ↔ 3281 Publication
Disulfide bondi487 ↔ 5211 Publication
Glycosylationi522N-linked (GlcNAc...)Sequence analysis1
Modified residuei714PhosphoserineCombined sources1
Modified residuei719PhosphoserineCombined sources1
Modified residuei770PhosphotyrosineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ69ZK9.
PaxDbiQ69ZK9.
PeptideAtlasiQ69ZK9.
PRIDEiQ69ZK9.

PTM databases

iPTMnetiQ69ZK9.
PhosphoSitePlusiQ69ZK9.

Expressioni

Tissue specificityi

Brain and arteries. Detected in the retina outer plexiform layer (at protein level). Widely expressed. Detected in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.5 Publications

Gene expression databases

BgeeiENSMUSG00000051790.
CleanExiMM_NLGN2.
ExpressionAtlasiQ69ZK9. baseline and differential.
GenevisibleiQ69ZK9. MM.

Interactioni

Subunit structurei

Interacts with NRXN1, NRXN2 and NRXN3. Interacts (via its C-terminus) with DLG4/PSD-95 (via PDZ domain 3). Interacts with PATJ (By similarity). Interacts with MDGA2 (By similarity). Interacts with GPHN (PubMed:19755106). Interacts with MDGA1 (PubMed:23358245). Found in a complex with MAGI2 and IGSF9B, where it interacts with MAGI2 (via WW 1, WW 2 and PDZ 2 domains) (PubMed:23751499).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PTPRTO145222EBI-775065,EBI-728180From a different organism.

GO - Molecular functioni

  • cell adhesion molecule binding Source: BHF-UCL
  • neurexin family protein binding Source: BHF-UCL

Protein-protein interaction databases

DIPiDIP-29702N.
IntActiQ69ZK9. 3 interactors.
STRINGi10090.ENSMUSP00000053097.

Structurei

Secondary structure

1836
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi59 – 63Combined sources5
Beta strandi66 – 73Combined sources8
Turni81 – 84Combined sources4
Beta strandi94 – 97Combined sources4
Beta strandi110 – 112Combined sources3
Turni116 – 118Combined sources3
Helixi121 – 125Combined sources5
Helixi127 – 131Combined sources5
Helixi132 – 134Combined sources3
Beta strandi135 – 137Combined sources3
Beta strandi143 – 149Combined sources7
Beta strandi151 – 154Combined sources4
Beta strandi177 – 183Combined sources7
Beta strandi186 – 190Combined sources5
Helixi193 – 195Combined sources3
Helixi199 – 205Combined sources7
Beta strandi207 – 212Combined sources6
Helixi217 – 221Combined sources5
Helixi233 – 248Combined sources16
Helixi249 – 252Combined sources4
Beta strandi254 – 264Combined sources11
Helixi266 – 276Combined sources11
Beta strandi281 – 283Combined sources3
Beta strandi286 – 291Combined sources6
Beta strandi294 – 296Combined sources3
Turni297 – 299Combined sources3
Helixi304 – 315Combined sources12
Helixi322 – 329Combined sources8
Helixi334 – 338Combined sources5
Beta strandi350 – 352Combined sources3
Beta strandi357 – 360Combined sources4
Helixi364 – 370Combined sources7
Beta strandi377 – 383Combined sources7
Helixi390 – 394Combined sources5
Beta strandi396 – 400Combined sources5
Helixi403 – 416Combined sources14
Helixi424 – 435Combined sources12
Helixi444 – 459Combined sources16
Helixi461 – 473Combined sources13
Beta strandi478 – 484Combined sources7
Turni500 – 503Combined sources4
Helixi504 – 508Combined sources5
Helixi510 – 513Combined sources4
Beta strandi517 – 519Combined sources3
Helixi525 – 544Combined sources20
Beta strandi549 – 551Combined sources3
Helixi564 – 566Combined sources3
Beta strandi575 – 577Combined sources3
Beta strandi579 – 586Combined sources8
Beta strandi589 – 592Combined sources4
Helixi595 – 602Combined sources8
Turni603 – 605Combined sources3
Helixi606 – 608Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BL8X-ray3.30A/B/C/D42-612[»]
ProteinModelPortaliQ69ZK9.
SMRiQ69ZK9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ69ZK9.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1516. Eukaryota.
COG2272. LUCA.
GeneTreeiENSGT00760000118946.
HOGENOMiHOG000231424.
HOVERGENiHBG008839.
InParanoidiQ69ZK9.
KOiK07378.
OMAiGCNQEEN.
OrthoDBiEOG091G0CHW.
PhylomeDBiQ69ZK9.
TreeFamiTF326187.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019819. Carboxylesterase_B_CS.
IPR000460. Nlgn.
IPR030023. NLGN2.
[Graphical view]
PANTHERiPTHR11559:SF53. PTHR11559:SF53. 2 hits.
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
PRINTSiPR01090. NEUROLIGIN.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q69ZK9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWLLALCLVG LAGAQRGGGG PGGGAPGGPG LGLGSLGEER FPVVNTAYGR
60 70 80 90 100
VRGVRRELNN EILGPVVQFL GVPYATPPLG ARRFQPPEAP ASWPGVRNAT
110 120 130 140 150
TLPPACPQNL HGALPAIMLP VWFTDNLEAA ATYVQNQSED CLYLNLYVPT
160 170 180 190 200
EDGPLTKKRD EATLNPPDTD IRDSGKKPVM LFLHGGSYME GTGNMFDGSV
210 220 230 240 250
LAAYGNVIVV TLNYRLGVLG FLSTGDQAAK GNYGLLDQIQ ALRWLSENIA
260 270 280 290 300
HFGGDPERIT IFGSGAGASC VNLLILSHHS EGLFQKAIAQ SGTAISSWSV
310 320 330 340 350
NYQPLKYTRL LAAKVGCDRE DSTEAVECLR RKSSRELVDQ DVQPARYHIA
360 370 380 390 400
FGPVVDGDVV PDDPEILMQQ GEFLNYDMLI GVNQGEGLKF VEDSAESEDG
410 420 430 440 450
VSASAFDFTV SNFVDNLYGY PEGKDVLRET IKFMYTDWAD RDNGEMRRKT
460 470 480 490 500
LLALFTDHQW VAPAVATAKL HADYQSPVYF YTFYHHCQAE GRPEWADAAH
510 520 530 540 550
GDELPYVFGV PMVGATDLFP CNFSKNDVML SAVVMTYWTN FAKTGDPNQP
560 570 580 590 600
VPQDTKFIHT KPNRFEEVVW SKFNSKEKQY LHIGLKPRVR DNYRANKVAF
610 620 630 640 650
WLELVPHLHN LHTELFTTTT RLPPYATRWP PRTPGPGTSG TRRPPPPATL
660 670 680 690 700
PPESDIDLGP RAYDRFPGDS RDYSTELSVT VAVGASLLFL NILAFAALYY
710 720 730 740 750
KRDRRQELRC RRLSPPGGSG SGVPGGGPLL PTAGRELPPE EELVSLQLKR
760 770 780 790 800
GGGVGADPAE ALRPACPPDY TLALRRAPDD VPLLAPGALT LLPSGLGPPP
810 820 830
PPPPPSLHPF GPFPPPPPTA TSHNNTLPHP HSTTRV
Length:836
Mass (Da):90,989
Last modified:September 13, 2005 - v2
Checksum:i4C03297F4F1A1F14
GO

Sequence cautioni

The sequence BAD32437 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti210V → A in BAD32437 (PubMed:15368895).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK173159 mRNA. Translation: BAD32437.1. Different initiation.
AL603707 Genomic DNA. Translation: CAI51964.1.
CCDSiCCDS24914.1.
RefSeqiNP_942562.2. NM_198862.2.
XP_006532964.1. XM_006532901.2.
UniGeneiMm.151293.

Genome annotation databases

EnsembliENSMUST00000056484; ENSMUSP00000053097; ENSMUSG00000051790.
ENSMUST00000108634; ENSMUSP00000104274; ENSMUSG00000051790.
GeneIDi216856.
KEGGimmu:216856.
UCSCiuc007jrw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK173159 mRNA. Translation: BAD32437.1. Different initiation.
AL603707 Genomic DNA. Translation: CAI51964.1.
CCDSiCCDS24914.1.
RefSeqiNP_942562.2. NM_198862.2.
XP_006532964.1. XM_006532901.2.
UniGeneiMm.151293.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BL8X-ray3.30A/B/C/D42-612[»]
ProteinModelPortaliQ69ZK9.
SMRiQ69ZK9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29702N.
IntActiQ69ZK9. 3 interactors.
STRINGi10090.ENSMUSP00000053097.

Protein family/group databases

ESTHERimouse-2neur. Neuroligin.

PTM databases

iPTMnetiQ69ZK9.
PhosphoSitePlusiQ69ZK9.

Proteomic databases

MaxQBiQ69ZK9.
PaxDbiQ69ZK9.
PeptideAtlasiQ69ZK9.
PRIDEiQ69ZK9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000056484; ENSMUSP00000053097; ENSMUSG00000051790.
ENSMUST00000108634; ENSMUSP00000104274; ENSMUSG00000051790.
GeneIDi216856.
KEGGimmu:216856.
UCSCiuc007jrw.1. mouse.

Organism-specific databases

CTDi57555.
MGIiMGI:2681835. Nlgn2.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1516. Eukaryota.
COG2272. LUCA.
GeneTreeiENSGT00760000118946.
HOGENOMiHOG000231424.
HOVERGENiHBG008839.
InParanoidiQ69ZK9.
KOiK07378.
OMAiGCNQEEN.
OrthoDBiEOG091G0CHW.
PhylomeDBiQ69ZK9.
TreeFamiTF326187.

Enzyme and pathway databases

ReactomeiR-MMU-6794361. Interactions of neurexins and neuroligins at synapses.

Miscellaneous databases

ChiTaRSiNlgn2. mouse.
EvolutionaryTraceiQ69ZK9.
PROiQ69ZK9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000051790.
CleanExiMM_NLGN2.
ExpressionAtlasiQ69ZK9. baseline and differential.
GenevisibleiQ69ZK9. MM.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019819. Carboxylesterase_B_CS.
IPR000460. Nlgn.
IPR030023. NLGN2.
[Graphical view]
PANTHERiPTHR11559:SF53. PTHR11559:SF53. 2 hits.
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
PRINTSiPR01090. NEUROLIGIN.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNLGN2_MOUSE
AccessioniPrimary (citable) accession number: Q69ZK9
Secondary accession number(s): Q5F288
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: November 30, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.