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Protein

Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein

Gene

Prex1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a RAC guanine nucleotide exchange factor (GEF), which activates the Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. May function downstream of heterotrimeric G proteins in neutrophils (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • G-protein coupled receptor signaling pathway Source: MGI
  • intracellular signal transduction Source: InterPro
  • neutrophil chemotaxis Source: MGI
  • positive regulation of cell adhesion Source: MGI
  • positive regulation of GTPase activity Source: MGI
  • positive regulation of substrate adhesion-dependent cell spreading Source: MGI
  • reactive oxygen species metabolic process Source: MGI
  • regulation of actin filament polymerization Source: Ensembl
  • regulation of dendrite development Source: Ensembl
  • regulation of Rho protein signal transduction Source: InterPro
  • T cell differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein
Short name:
P-Rex1
Short name:
PtdIns(3,4,5)-dependent Rac exchanger 1
Gene namesi
Name:Prex1
Synonyms:Kiaa1415
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:3040696. Prex1.

Subcellular locationi

  • Cytoplasmcytosol
  • Cell membrane

  • Note: Mainly cytosolic. Some amount is apparently associated to the plasma membrane (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 16501650Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 proteinPRO_0000292674Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei314 – 3141PhosphoserineBy similarity
Modified residuei991 – 9911PhosphoserineCombined sources
Modified residuei1186 – 11861PhosphoserineCombined sources
Modified residuei1191 – 11911PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ69ZK0.
MaxQBiQ69ZK0.
PaxDbiQ69ZK0.
PeptideAtlasiQ69ZK0.
PRIDEiQ69ZK0.

PTM databases

iPTMnetiQ69ZK0.
PhosphoSiteiQ69ZK0.

Expressioni

Gene expression databases

BgeeiQ69ZK0.
CleanExiMM_BC067047.
ExpressionAtlasiQ69ZK0. baseline and differential.
GenevisibleiQ69ZK0. MM.

Interactioni

Subunit structurei

Interacts preferentially with RAC2. Interacts with RAC1.1 Publication

Protein-protein interaction databases

IntActiQ69ZK0. 3 interactions.
STRINGi10090.ENSMUSP00000037180.

Structurei

3D structure databases

ProteinModelPortaliQ69ZK0.
SMRiQ69ZK0. Positions 30-394, 602-701.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini44 – 235192DHPROSITE-ProRule annotationAdd
BLAST
Domaini266 – 387122PHPROSITE-ProRule annotationAdd
BLAST
Domaini416 – 49176DEP 1PROSITE-ProRule annotationAdd
BLAST
Domaini518 – 59275DEP 2PROSITE-ProRule annotationAdd
BLAST
Domaini620 – 69879PDZPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1469 – 14724Poly-Pro

Sequence similaritiesi

Contains 2 DEP domains.PROSITE-ProRule annotation
Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410ITRX. Eukaryota.
ENOG410Z6ZS. LUCA.
GeneTreeiENSGT00760000118925.
HOGENOMiHOG000115690.
HOVERGENiHBG053677.
InParanoidiQ69ZK0.
KOiK12365.
OMAiQCILKVN.
OrthoDBiEOG7V49XH.
PhylomeDBiQ69ZK0.
TreeFamiTF328639.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
2.30.42.10. 2 hits.
InterProiIPR000591. DEP_dom.
IPR000219. DH-domain.
IPR001331. GDS_CDC24_CS.
IPR001478. PDZ.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00610. DEP. 2 hits.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTiSM00049. DEP. 2 hits.
SM00228. PDZ. 2 hits.
SM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 2 hits.
SSF48065. SSF48065. 1 hit.
SSF50156. SSF50156. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50186. DEP. 2 hits.
PS00741. DH_1. 1 hit.
PS50010. DH_2. 1 hit.
PS50106. PDZ. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q69ZK0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEAPGSGGGD GGGDPGGDGA HPDARGPVSG PCAAARDSER QLRLRLCVLN
60 70 80 90 100
EILGTERDYV GTLRFLQSAF LQRIRQNVAD SVEKGLTEEN VKVLFSNIED
110 120 130 140 150
ILEVHKDFLA ALEYCLHPEP QSQHELGNVF LKFKDKFCVY EEYCSNHEKA
160 170 180 190 200
LRLLVELNKV PAVRAFLLSC MLLGGRKTTD IPLEGYLLSP IQRICKYPLL
210 220 230 240 250
LKELAKRTPG KHPDHTAVQS ALQAMKTVCS NINETKRQME KLEALEQLQS
260 270 280 290 300
HIEGWEGSNL TDICTELLLQ GNLLKISAGN IQERAFFLFD NLLVYCKRKS
310 320 330 340 350
RVTGSKKSTK RTKSINGSLY IFRGRINTEV MEVENVEDGT ADYHSNGYTV
360 370 380 390 400
TNGWKIHNTA KNKWFVCMAK TAEEKQKWLD ALIREREQRE SLKLGMERDA
410 420 430 440 450
YVMIAEKGEK LYHMMMSKKV NLIKDRRRKL STVPKCFLGN EFVAWLLEIG
460 470 480 490 500
EISKTEEGVN LGQALLENGI IHHVSDKHQF KNEQVMYRFR YDDGTYKARS
510 520 530 540 550
ELEDIMSKGV RLYCRLHSLY APVIKDRDYH LKTYKSVVPG SKLVDWLLAQ
560 570 580 590 600
GDCQTREEAV ALGVGLCNNG FMHHVLEKSE FKDESQYFRF HADEEMEGTS
610 620 630 640 650
SKNKQLRNDF KLVENILAKR LLIPPQEDDY GFDLEEKNKA VVVKSVQRGS
660 670 680 690 700
LAEMAGLQAG RKIYSINEDL VFLRPFSEVE TILNQFFCSR RPLRLLVATK
710 720 730 740 750
AKETIKVPDH PEALSFQIRG TAPPCVFAVG RGSEAVAAGL CAGQCILKVN
760 770 780 790 800
GTSVANDGAL EVLEHFQAFR NHREEALGLY QWVYHSHEDA QLARASQGAP
810 820 830 840 850
DEDPQEDDQP DSALPLLSLG PQLSLHEDSA VVSLTLDNVH LEHGVVYEYM
860 870 880 890 900
STAGAKCHVL EKIVEPRGCF RLAAKILEAF AVDDSIFVQN CGRLMAMSSA
910 920 930 940 950
IVTMSHYEFH NICDTKLESI GQRIACYQEF AAQLKSRVSP PFKQASLEPH
960 970 980 990 1000
PLCGLDFCPT NCHVNLMEVS YPKTTPSVGR SFSIRFGRKP SLIGLDPEQG
1010 1020 1030 1040 1050
LNPMAYTQHC ITTMAAPSWK CSPAVDEDSQ GQGLNDSSYG SASGAPSQQD
1060 1070 1080 1090 1100
RGLSFLLKQE DREIQDAYLQ LFTKLDVALK EMKQYVTQIN RLLSTITEPT
1110 1120 1130 1140 1150
SAAPAPCDPS LVEETSSSPP VSEESEVDRT DHSGIKKVCF KVSEDEQEDS
1160 1170 1180 1190 1200
GHDTMSYRDS YSECNSNRDS VLSYTSVRSN SSYLGSDEMG SGDELPCDMR
1210 1220 1230 1240 1250
IPSDKQDKLH GCLEHLFNQV DSIHALLKGP VMSRAFEETR HFPMKHSWQE
1260 1270 1280 1290 1300
FKQKEECTVR GRNLIQISIQ EDPWNLPSSI RTLVDNIQQY VEDGKNQLLL
1310 1320 1330 1340 1350
ALLKCTDTEL QLRRDAVFCQ ALVAAVCTFS EQLLAALDYR YNNNGEYEES
1360 1370 1380 1390 1400
SRDASRKWLE QVAATGVLLH WQSLLAPASV KEERTMLEDI WVTLSELDNV
1410 1420 1430 1440 1450
TFSFKQLDEN SVANTNVFYH IEGSRQALKV VFYLDGFHFS RLPSRLEGGA
1460 1470 1480 1490 1500
SLRLHTVLFT KALESVEGPP PPGNQAAEEL QQEINAQSLE KVQQYYRKLR
1510 1520 1530 1540 1550
AFYLERSNLP TDAGATAVKI DQLIRPINAL DELYRLMKTF VHPKAGAAGS
1560 1570 1580 1590 1600
LGAGLIPVSS ELCYRLGACQ ITMCGTGMQR STLSVSLEQA AILARSHGLL
1610 1620 1630 1640 1650
PKCVMQATDI MRKQGPRVEI LAKNLRIKDP MPQGAPRLYQ LCQPPVDGDL
Length:1,650
Mass (Da):184,935
Last modified:June 26, 2007 - v2
Checksum:iEB1C0140126209FC
GO
Isoform 2 (identifier: Q69ZK0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1188: Missing.

Show »
Length:462
Mass (Da):51,898
Checksum:i3C66B9F7533B4546
GO

Sequence cautioni

The sequence BAD32446.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1575 – 15751G → V in AAH57617 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11881188Missing in isoform 2. 1 PublicationVSP_026436Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK173168 mRNA. Translation: BAD32446.1. Different initiation.
AL591884 Genomic DNA. Translation: CAM16778.1.
AL591884, AL732357 Genomic DNA. Translation: CAM16779.1.
AL732357, AL591884 Genomic DNA. Translation: CAM22699.1.
AK090301 mRNA. Translation: BAC41161.1.
BC057617 mRNA. Translation: AAH57617.1.
CCDSiCCDS38334.1. [Q69ZK0-1]
RefSeqiNP_808450.2. NM_177782.3. [Q69ZK0-1]
UniGeneiMm.34420.
Mm.491336.

Genome annotation databases

EnsembliENSMUST00000036719; ENSMUSP00000037180; ENSMUSG00000039621. [Q69ZK0-1]
ENSMUST00000109246; ENSMUSP00000104869; ENSMUSG00000039621. [Q69ZK0-2]
GeneIDi277360.
KEGGimmu:277360.
UCSCiuc008nym.1. mouse. [Q69ZK0-2]
uc008nyn.1. mouse. [Q69ZK0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK173168 mRNA. Translation: BAD32446.1. Different initiation.
AL591884 Genomic DNA. Translation: CAM16778.1.
AL591884, AL732357 Genomic DNA. Translation: CAM16779.1.
AL732357, AL591884 Genomic DNA. Translation: CAM22699.1.
AK090301 mRNA. Translation: BAC41161.1.
BC057617 mRNA. Translation: AAH57617.1.
CCDSiCCDS38334.1. [Q69ZK0-1]
RefSeqiNP_808450.2. NM_177782.3. [Q69ZK0-1]
UniGeneiMm.34420.
Mm.491336.

3D structure databases

ProteinModelPortaliQ69ZK0.
SMRiQ69ZK0. Positions 30-394, 602-701.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ69ZK0. 3 interactions.
STRINGi10090.ENSMUSP00000037180.

PTM databases

iPTMnetiQ69ZK0.
PhosphoSiteiQ69ZK0.

Proteomic databases

EPDiQ69ZK0.
MaxQBiQ69ZK0.
PaxDbiQ69ZK0.
PeptideAtlasiQ69ZK0.
PRIDEiQ69ZK0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036719; ENSMUSP00000037180; ENSMUSG00000039621. [Q69ZK0-1]
ENSMUST00000109246; ENSMUSP00000104869; ENSMUSG00000039621. [Q69ZK0-2]
GeneIDi277360.
KEGGimmu:277360.
UCSCiuc008nym.1. mouse. [Q69ZK0-2]
uc008nyn.1. mouse. [Q69ZK0-1]

Organism-specific databases

CTDi57580.
MGIiMGI:3040696. Prex1.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410ITRX. Eukaryota.
ENOG410Z6ZS. LUCA.
GeneTreeiENSGT00760000118925.
HOGENOMiHOG000115690.
HOVERGENiHBG053677.
InParanoidiQ69ZK0.
KOiK12365.
OMAiQCILKVN.
OrthoDBiEOG7V49XH.
PhylomeDBiQ69ZK0.
TreeFamiTF328639.

Miscellaneous databases

PROiQ69ZK0.
SOURCEiSearch...

Gene expression databases

BgeeiQ69ZK0.
CleanExiMM_BC067047.
ExpressionAtlasiQ69ZK0. baseline and differential.
GenevisibleiQ69ZK0. MM.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
2.30.42.10. 2 hits.
InterProiIPR000591. DEP_dom.
IPR000219. DH-domain.
IPR001331. GDS_CDC24_CS.
IPR001478. PDZ.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00610. DEP. 2 hits.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTiSM00049. DEP. 2 hits.
SM00228. PDZ. 2 hits.
SM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 2 hits.
SSF48065. SSF48065. 1 hit.
SSF50156. SSF50156. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50186. DEP. 2 hits.
PS00741. DH_1. 1 hit.
PS50010. DH_2. 1 hit.
PS50106. PDZ. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
    DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Pancreatic islet.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Ovary and Uterus.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1144-1650 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Fetal brain.
  5. "P-Rex1 is a primary Rac2 guanine nucleotide exchange factor in mouse neutrophils."
    Dong X., Mo Z., Bokoch G., Guo C., Li Z., Wu D.
    Curr. Biol. 15:1874-1879(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RAC1 AND RAC2.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-991; SER-1186 AND SER-1191, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Lung and Spleen.

Entry informationi

Entry nameiPREX1_MOUSE
AccessioniPrimary (citable) accession number: Q69ZK0
Secondary accession number(s): A2A5U1, Q6PFD4, Q8BN08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: June 26, 2007
Last modified: July 6, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.