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Protein

Histone H2A deubiquitinase MYSM1

Gene

Mysm1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Preferentially deubiquitinates monoubiquitinated H2A in hyperacetylated nucleosomes. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. Acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi647 – 6471Zinc; catalyticBy similarity
Metal bindingi649 – 6491Zinc; catalyticBy similarity
Metal bindingi660 – 6601Zinc; catalyticBy similarity

GO - Molecular functioni

GO - Biological processi

  • chromatin remodeling Source: UniProtKB
  • monoubiquitinated histone H2A deubiquitination Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • regulation of cell migration Source: MGI
  • regulation of hair follicle development Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator, Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Transcription, Transcription regulation, Ubl conjugation pathway

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Protein family/group databases

MEROPSiM67.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone H2A deubiquitinase MYSM1 (EC:3.4.19.-)
Short name:
2A-DUB
Alternative name(s):
Myb-like, SWIRM and MPN domain-containing protein 1
Gene namesi
Name:Mysm1
Synonyms:Kiaa1915
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2444584. Mysm1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 819819Histone H2A deubiquitinase MYSM1PRO_0000234074Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei215 – 2151PhosphoserineBy similarity
Modified residuei233 – 2331PhosphothreonineBy similarity
Modified residuei332 – 3321PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ69Z66.
MaxQBiQ69Z66.
PaxDbiQ69Z66.
PRIDEiQ69Z66.

PTM databases

iPTMnetiQ69Z66.
PhosphoSiteiQ69Z66.

Expressioni

Gene expression databases

BgeeiQ69Z66.
CleanExiMM_MYSM1.
GenevisibleiQ69Z66. MM.

Interactioni

Subunit structurei

Component of a large chromatin remodeling complex, at least composed of MYSM1, PCAF, RBM10 and KIF11/TRIP5. Binds histones (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi236236. 3 interactions.
STRINGi10090.ENSMUSP00000075269.

Structurei

3D structure databases

ProteinModelPortaliQ69Z66.
SMRiQ69Z66. Positions 116-177, 358-461, 568-685.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini113 – 16452SANTAdd
BLAST
Domaini363 – 46199SWIRMPROSITE-ProRule annotationAdd
BLAST
Domaini563 – 673111MPNAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi647 – 66014JAMM motifAdd
BLAST
Motifi765 – 7695LXXLL motif

Domaini

Binds double-stranded DNA via the SANT domain. The SWIRM domain does not bind double-stranded DNA (By similarity).By similarity

Sequence similaritiesi

Belongs to the peptidase M67A family. MYSM1 subfamily.Curated
Contains 1 MPN (JAB/Mov34) domain.Curated
Contains 1 SANT domain.Curated
Contains 1 SWIRM domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1279. Eukaryota.
KOG1555. Eukaryota.
COG1310. LUCA.
GeneTreeiENSGT00530000063766.
HOGENOMiHOG000113713.
HOVERGENiHBG079486.
InParanoidiQ69Z66.
KOiK11865.
OMAiIPWTLDN.
OrthoDBiEOG7P8P96.
PhylomeDBiQ69Z66.
TreeFamiTF324811.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.10.60. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR000555. JAMM/MPN+_dom.
IPR017930. Myb_dom.
IPR001005. SANT/Myb.
IPR007526. SWIRM.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01398. JAB. 1 hit.
PF00249. Myb_DNA-binding. 1 hit.
PF04433. SWIRM. 1 hit.
[Graphical view]
SMARTiSM00232. JAB_MPN. 1 hit.
SM00717. SANT. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
PROSITEiPS50934. SWIRM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q69Z66-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEAEEADVDV EGDVAAAAQP GNDESTASVF QDHYLDSTWR RENGCLPWTL
60 70 80 90 100
DSTISDENRA IIEKMLLEEE YYLSNKSLPG KFWVNQKEDN KKYTNSLQKS
110 120 130 140 150
SKAMVDSPAK PASHSVKWTV EEKELFEQGL AKFGRRWTKI ATLLKSRTVL
160 170 180 190 200
QVKSYARQYF KNKVKWDVEK ETPTQKSSSD LQVKNKDDRT KAWAAACLRG
210 220 230 240 250
SADPCLNAVK IEKLSDDEDV DITDELDELT SQTSQNSGSH LTLDVPNSKM
260 270 280 290 300
YTTNQGELCQ EGPLAKSSGE SLQNVKQGEG EACSSSEIAS WAEKQKSTDK
310 320 330 340 350
NSAELNEKYN KVVEEHTLHR GEVREEAKHS PSPEPCERQD SSGNEMLLPP
360 370 380 390 400
CQIEEENHEG EELKPPEQEV EIDRNVIQEE EKQAIPEFFE GRQTKTPERY
410 420 430 440 450
LKIRNYILDQ WEICKPKYLN KTSVRPGLKN CGDVNCIGRI HTYLELIGAI
460 470 480 490 500
NFGCEQAVYN RPQPLDKVRA ADRKDAEAAY QLAWRLQSMR TRRRRVRDPW
510 520 530 540 550
GNWCDAKDLE GQTFEHLSVE EMARRKEEEK CKPIKFSKAS KLPKSSLDPF
560 570 580 590 600
QLIPCNFFSE EKQEPFQVKV AAEALLIMNL HAHVSMAEVI GLLGGRYSEA
610 620 630 640 650
DKVLEVCAAE PCNSLSTGLQ CEMDPVSQTQ ASETLALRGY SVIGWYHSHP
660 670 680 690 700
AFDPNPSLRD IDTQAKYQSY FSRGGAKFIG MIVSPYNRSN PLPYSQITCL
710 720 730 740 750
VISEEVSPDG TYRLPYKFEV QQMLEEPQWE LVFEKTRWII EKYRLSNSSV
760 770 780 790 800
PMDRIFRRDS DLTCLQKLLE CLRKTLSKVA NCFIAEEFLT QIENLFLSNY
810
KSKEENGLAE EDSTKELFM
Note: No experimental confirmation available.
Length:819
Mass (Da):93,475
Last modified:May 2, 2006 - v2
Checksum:iB5A720311CD9C779
GO
Isoform 2 (identifier: Q69Z66-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     412-819: Missing.

Note: No experimental confirmation available.
Show »
Length:411
Mass (Da):46,702
Checksum:i7C2A5EF9CDD447DD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti7 – 71D → G in BAE37628 (PubMed:16141072).Curated
Sequence conflicti175 – 1751Q → H in BAC31657 (PubMed:16141072).Curated
Sequence conflicti221 – 2211D → H in BAE37628 (PubMed:16141072).Curated
Sequence conflicti265 – 2651A → P in BAE37628 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei412 – 819408Missing in isoform 2. 1 PublicationVSP_018212Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK043802 mRNA. Translation: BAC31657.1.
AK081684 mRNA. Translation: BAC38291.1.
AK164103 mRNA. Translation: BAE37628.1.
AL732611 Genomic DNA. Translation: CAM14751.1.
BC150946 mRNA. Translation: AAI50947.1.
BC151172 mRNA. Translation: AAI51173.1.
AK173300 mRNA. Translation: BAD32578.1.
CCDSiCCDS18363.1. [Q69Z66-1]
RefSeqiNP_796213.2. NM_177239.2. [Q69Z66-1]
UniGeneiMm.208868.
Mm.468748.

Genome annotation databases

EnsembliENSMUST00000075872; ENSMUSP00000075269; ENSMUSG00000062627. [Q69Z66-1]
GeneIDi320713.
KEGGimmu:320713.
UCSCiuc008tsn.1. mouse. [Q69Z66-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK043802 mRNA. Translation: BAC31657.1.
AK081684 mRNA. Translation: BAC38291.1.
AK164103 mRNA. Translation: BAE37628.1.
AL732611 Genomic DNA. Translation: CAM14751.1.
BC150946 mRNA. Translation: AAI50947.1.
BC151172 mRNA. Translation: AAI51173.1.
AK173300 mRNA. Translation: BAD32578.1.
CCDSiCCDS18363.1. [Q69Z66-1]
RefSeqiNP_796213.2. NM_177239.2. [Q69Z66-1]
UniGeneiMm.208868.
Mm.468748.

3D structure databases

ProteinModelPortaliQ69Z66.
SMRiQ69Z66. Positions 116-177, 358-461, 568-685.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi236236. 3 interactions.
STRINGi10090.ENSMUSP00000075269.

Protein family/group databases

MEROPSiM67.005.

PTM databases

iPTMnetiQ69Z66.
PhosphoSiteiQ69Z66.

Proteomic databases

EPDiQ69Z66.
MaxQBiQ69Z66.
PaxDbiQ69Z66.
PRIDEiQ69Z66.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000075872; ENSMUSP00000075269; ENSMUSG00000062627. [Q69Z66-1]
GeneIDi320713.
KEGGimmu:320713.
UCSCiuc008tsn.1. mouse. [Q69Z66-1]

Organism-specific databases

CTDi114803.
MGIiMGI:2444584. Mysm1.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1279. Eukaryota.
KOG1555. Eukaryota.
COG1310. LUCA.
GeneTreeiENSGT00530000063766.
HOGENOMiHOG000113713.
HOVERGENiHBG079486.
InParanoidiQ69Z66.
KOiK11865.
OMAiIPWTLDN.
OrthoDBiEOG7P8P96.
PhylomeDBiQ69Z66.
TreeFamiTF324811.

Miscellaneous databases

NextBioi397281.
PROiQ69Z66.
SOURCEiSearch...

Gene expression databases

BgeeiQ69Z66.
CleanExiMM_MYSM1.
GenevisibleiQ69Z66. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.10.60. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR000555. JAMM/MPN+_dom.
IPR017930. Myb_dom.
IPR001005. SANT/Myb.
IPR007526. SWIRM.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01398. JAB. 1 hit.
PF00249. Myb_DNA-binding. 1 hit.
PF04433. SWIRM. 1 hit.
[Graphical view]
SMARTiSM00232. JAB_MPN. 1 hit.
SM00717. SANT. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
PROSITEiPS50934. SWIRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-354.
    Strain: C57BL/6J.
    Tissue: Brain cortex, Head and Spinal cord.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
    DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 75-819.
    Tissue: Pancreatic islet.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Pancreas and Spleen.

Entry informationi

Entry nameiMYSM1_MOUSE
AccessioniPrimary (citable) accession number: Q69Z66
Secondary accession number(s): A2AJE3
, B9EKJ6, Q3TPV7, Q8BRP5, Q8C4N1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: May 2, 2006
Last modified: May 11, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.