Q69Z66 (MYSM1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histone H2A deubiquitinase MYSM1
Short name=2A-DUB
EC=3.4.19.-

Alternative name(s):
Myb-like, SWIRM and MPN domain-containing protein 1

Gene names

Name:	Mysm1
Synonyms:	Kiaa1915

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	819 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Preferentially deubiquitinates monoubiquitinated H2A in hyperacetylated nucleosomes. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. Acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation By similarity.
Subunit structure	Component of a large chromatin remodeling complex, at least composed of MYSM1, PCAF, RBM10 and KIF11/TRIP5. Binds histones By similarity.
Subcellular location	Nucleus Probable.
Domain	Binds double-stranded DNA via the SANT domain. The SWIRM domain does not bind double-stranded DNA By similarity.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR By similarity.
Sequence similarities	Belongs to the peptidase M67A family. MYSM1 subfamily. Contains 1 MPN (JAB/Mov34) domain. Contains 1 SANT domain. Contains 1 SWIRM domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Ubl conjugation pathway
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator Chromatin regulator Hydrolase Metalloprotease Protease
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	histone deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB proteolysis Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromatin remodeling complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW histone binding Inferred from sequence or structural similarity. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metallopeptidase activity Inferred from sequence or structural similarity. Source: UniProtKB transcription coactivator activity Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin thiolesterase activity Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-specific protease activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q69Z66-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 (identifier: Q69Z66-2)

The sequence of this isoform differs from the canonical sequence as follows:
     412-819: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 819	819	Histone H2A deubiquitinase MYSM1		PRO_0000234074
Regions
	Domain	113 – 164	52	SANT
	Domain	363 – 461	99	SWIRM
	Domain	563 – 673	111	MPN
	Motif	647 – 660	14	JAMM motif
	Motif	765 – 769	5	LXXLL motif
Sites
	Metal binding	647	1	Zinc; catalytic By similarity
	Metal binding	649	1	Zinc; catalytic By similarity
	Metal binding	660	1	Zinc; catalytic By similarity
Amino acid modifications
	Modified residue	215	1	Phosphoserine By similarity
	Modified residue	231	1	Phosphoserine By similarity
	Modified residue	233	1	Phosphothreonine By similarity
Natural variations
	Alternative sequence	412 – 819	408	Missing in isoform 2.		VSP_018212
Experimental info
	Sequence conflict	7	1	D → G in BAE37628. Ref.1
	Sequence conflict	175	1	Q → H in BAC31657. Ref.1
	Sequence conflict	221	1	D → H in BAE37628. Ref.1
	Sequence conflict	265	1	A → P in BAE37628. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 2, 2006. Version 2. Checksum: B5A720311CD9C779	FASTA	819	93,475
	10 20 30 40 50 60 MEAEEADVDV EGDVAAAAQP GNDESTASVF QDHYLDSTWR RENGCLPWTL DSTISDENRA 70 80 90 100 110 120 IIEKMLLEEE YYLSNKSLPG KFWVNQKEDN KKYTNSLQKS SKAMVDSPAK PASHSVKWTV 130 140 150 160 170 180 EEKELFEQGL AKFGRRWTKI ATLLKSRTVL QVKSYARQYF KNKVKWDVEK ETPTQKSSSD 190 200 210 220 230 240 LQVKNKDDRT KAWAAACLRG SADPCLNAVK IEKLSDDEDV DITDELDELT SQTSQNSGSH 250 260 270 280 290 300 LTLDVPNSKM YTTNQGELCQ EGPLAKSSGE SLQNVKQGEG EACSSSEIAS WAEKQKSTDK 310 320 330 340 350 360 NSAELNEKYN KVVEEHTLHR GEVREEAKHS PSPEPCERQD SSGNEMLLPP CQIEEENHEG 370 380 390 400 410 420 EELKPPEQEV EIDRNVIQEE EKQAIPEFFE GRQTKTPERY LKIRNYILDQ WEICKPKYLN 430 440 450 460 470 480 KTSVRPGLKN CGDVNCIGRI HTYLELIGAI NFGCEQAVYN RPQPLDKVRA ADRKDAEAAY 490 500 510 520 530 540 QLAWRLQSMR TRRRRVRDPW GNWCDAKDLE GQTFEHLSVE EMARRKEEEK CKPIKFSKAS 550 560 570 580 590 600 KLPKSSLDPF QLIPCNFFSE EKQEPFQVKV AAEALLIMNL HAHVSMAEVI GLLGGRYSEA 610 620 630 640 650 660 DKVLEVCAAE PCNSLSTGLQ CEMDPVSQTQ ASETLALRGY SVIGWYHSHP AFDPNPSLRD 670 680 690 700 710 720 IDTQAKYQSY FSRGGAKFIG MIVSPYNRSN PLPYSQITCL VISEEVSPDG TYRLPYKFEV 730 740 750 760 770 780 QQMLEEPQWE LVFEKTRWII EKYRLSNSSV PMDRIFRRDS DLTCLQKLLE CLRKTLSKVA 790 800 810 NCFIAEEFLT QIENLFLSNY KSKEENGLAE EDSTKELFM
	Isoform 2 [UniParc]. Checksum: 7C2A5EF9CDD447DD	FASTA	411	46,702
	10 20 30 40 50 60 MEAEEADVDV EGDVAAAAQP GNDESTASVF QDHYLDSTWR RENGCLPWTL DSTISDENRA 70 80 90 100 110 120 IIEKMLLEEE YYLSNKSLPG KFWVNQKEDN KKYTNSLQKS SKAMVDSPAK PASHSVKWTV 130 140 150 160 170 180 EEKELFEQGL AKFGRRWTKI ATLLKSRTVL QVKSYARQYF KNKVKWDVEK ETPTQKSSSD 190 200 210 220 230 240 LQVKNKDDRT KAWAAACLRG SADPCLNAVK IEKLSDDEDV DITDELDELT SQTSQNSGSH 250 260 270 280 290 300 LTLDVPNSKM YTTNQGELCQ EGPLAKSSGE SLQNVKQGEG EACSSSEIAS WAEKQKSTDK 310 320 330 340 350 360 NSAELNEKYN KVVEEHTLHR GEVREEAKHS PSPEPCERQD SSGNEMLLPP CQIEEENHEG 370 380 390 400 410 EELKPPEQEV EIDRNVIQEE EKQAIPEFFE GRQTKTPERY LKIRNYILDQ W

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-354. Strain: C57BL/6J. Tissue: Brain cortex, Head and Spinal cord.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H. DNA Res. 11:205-218(2004) [PubMed: 15368895] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 75-819. Tissue: Pancreatic islet.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK043802 mRNA. Translation: BAC31657.1. AK081684 mRNA. Translation: BAC38291.1. AK164103 mRNA. Translation: BAE37628.1. AL732611 Genomic DNA. Translation: CAM14751.1. BC150946 mRNA. Translation: AAI50947.1. BC151172 mRNA. Translation: AAI51173.1. AK173300 mRNA. Translation: BAD32578.1.
IPI	IPI00225524. IPI00660704.
RefSeq	NP_796213.2. NM_177239.2.
UniGene	Mm.208868. Mm.468748.
3D structure databases
ProteinModelPortal	Q69Z66.
SMR	Q69Z66. Positions 116-177, 358-516, 569-704.
ModBase	Search...
Protein family/group databases
MEROPS	M67.005.
PTM databases
PhosphoSite	Q69Z66.
Proteomic databases
PRIDE	Q69Z66.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000075872; ENSMUSP00000075269; ENSMUSG00000062627.
GeneID	320713.
KEGG	mmu:320713.
UCSC	uc008tsn.1. mouse. uc008tso.1. mouse.
Organism-specific databases
CTD	114803.
MGI	MGI:2444584. Mysm1.
Rouge	Search...
Phylogenomic databases
eggNOG	roNOG04217.
GeneTree	ENSGT00530000063766.
HOGENOM	HBG714596.
HOVERGEN	HBG079486.
InParanoid	Q69Z66.
OMA	DKIFRRD.
OrthoDB	EOG4ZGPBQ.
PhylomeDB	Q69Z66.
Gene expression databases
ArrayExpress	Q69Z66.
Bgee	Q69Z66.
CleanEx	MM_MYSM1.
Genevestigator	Q69Z66.
GermOnline	ENSMUSG00000062627. Mus musculus.
Family and domain databases
InterPro	IPR009057. Homeodomain-like. IPR012287. Homeodomain-rel. IPR000555. Mov34_MPN_PAD1. IPR014778. Myb_DNA-bd. IPR001005. SANT_DNA-bd. IPR007526. SWIRM. IPR017930. Tscrpt_reg_HTH_Myb-type. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K11865.
Pfam	PF01398. Mov34. 1 hit. PF00249. Myb_DNA-binding. 1 hit. PF04433. SWIRM. 1 hit. [Graphical view]
SMART	SM00232. JAB_MPN. 1 hit. SM00717. SANT. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 2 hits.
PROSITE	PS51293. SANT. False negative. PS50934. SWIRM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	397281.
SOURCE	Search...

Entry information

Entry name

MYSM1_MOUSE

Accession

Primary (citable) accession number: Q69Z66
Secondary accession number(s): A2AJE3 , B9EKJ6, Q3TPV7, Q8BRP5, Q8C4N1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	May 2, 2006
Last modified:	November 16, 2011
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program