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Protein

Histone H2A deubiquitinase MYSM1

Gene

Mysm1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Preferentially deubiquitinates monoubiquitinated H2A in hyperacetylated nucleosomes. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. Acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi647Zinc; catalyticBy similarity1
Metal bindingi649Zinc; catalyticBy similarity1
Metal bindingi660Zinc; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

  • chromatin remodeling Source: UniProtKB
  • monoubiquitinated histone H2A deubiquitination Source: UniProtKB
  • pigmentation Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • regulation of cell migration Source: MGI
  • regulation of hair follicle development Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, Chromatin regulator, DNA-binding, Hydrolase, Metalloprotease, Protease
Biological processTranscription, Transcription regulation, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-5689901. Metalloprotease DUBs.

Protein family/group databases

MEROPSiM67.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone H2A deubiquitinase MYSM1 (EC:3.4.19.-)
Short name:
2A-DUB
Alternative name(s):
Myb-like, SWIRM and MPN domain-containing protein 1
Gene namesi
Name:Mysm1
Synonyms:Kiaa1915
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2444584. Mysm1.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002340741 – 819Histone H2A deubiquitinase MYSM1Add BLAST819

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei107PhosphoserineBy similarity1
Cross-linki184Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei215PhosphoserineBy similarity1
Modified residuei233PhosphothreonineBy similarity1
Modified residuei332PhosphoserineBy similarity1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ69Z66.
PeptideAtlasiQ69Z66.
PRIDEiQ69Z66.

PTM databases

iPTMnetiQ69Z66.
PhosphoSitePlusiQ69Z66.

Expressioni

Gene expression databases

BgeeiENSMUSG00000062627.
CleanExiMM_MYSM1.
GenevisibleiQ69Z66. MM.

Interactioni

Subunit structurei

Component of a large chromatin remodeling complex, at least composed of MYSM1, PCAF, RBM10 and KIF11/TRIP5. Binds histones (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi236236. 8 interactors.
STRINGi10090.ENSMUSP00000075269.

Structurei

3D structure databases

ProteinModelPortaliQ69Z66.
SMRiQ69Z66.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini113 – 164SANTAdd BLAST52
Domaini363 – 461SWIRMPROSITE-ProRule annotationAdd BLAST99
Domaini563 – 673MPNAdd BLAST111

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi647 – 660JAMM motifAdd BLAST14
Motifi765 – 769LXXLL motif5

Domaini

Binds double-stranded DNA via the SANT domain. The SWIRM domain does not bind double-stranded DNA (By similarity).By similarity

Sequence similaritiesi

Belongs to the peptidase M67A family. MYSM1 subfamily.Curated

Phylogenomic databases

eggNOGiKOG1279. Eukaryota.
KOG1555. Eukaryota.
COG1310. LUCA.
GeneTreeiENSGT00530000063766.
HOGENOMiHOG000113713.
HOVERGENiHBG079486.
InParanoidiQ69Z66.
KOiK11865.
OMAiQEPFQVK.
OrthoDBiEOG091G052R.
PhylomeDBiQ69Z66.
TreeFamiTF324811.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR009057. Homeobox-like.
IPR000555. JAMM/MPN+_dom.
IPR017930. Myb_dom.
IPR033497. MYSM1.
IPR001005. SANT/Myb.
IPR007526. SWIRM.
IPR011991. WHTH_DNA-bd_dom.
PANTHERiPTHR12802:SF75. PTHR12802:SF75. 1 hit.
PfamiView protein in Pfam
PF01398. JAB. 1 hit.
PF00249. Myb_DNA-binding. 1 hit.
PF04433. SWIRM. 1 hit.
SMARTiView protein in SMART
SM00232. JAB_MPN. 1 hit.
SM00717. SANT. 1 hit.
SUPFAMiSSF46689. SSF46689. 2 hits.
PROSITEiView protein in PROSITE
PS50934. SWIRM. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q69Z66-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEAEEADVDV EGDVAAAAQP GNDESTASVF QDHYLDSTWR RENGCLPWTL
60 70 80 90 100
DSTISDENRA IIEKMLLEEE YYLSNKSLPG KFWVNQKEDN KKYTNSLQKS
110 120 130 140 150
SKAMVDSPAK PASHSVKWTV EEKELFEQGL AKFGRRWTKI ATLLKSRTVL
160 170 180 190 200
QVKSYARQYF KNKVKWDVEK ETPTQKSSSD LQVKNKDDRT KAWAAACLRG
210 220 230 240 250
SADPCLNAVK IEKLSDDEDV DITDELDELT SQTSQNSGSH LTLDVPNSKM
260 270 280 290 300
YTTNQGELCQ EGPLAKSSGE SLQNVKQGEG EACSSSEIAS WAEKQKSTDK
310 320 330 340 350
NSAELNEKYN KVVEEHTLHR GEVREEAKHS PSPEPCERQD SSGNEMLLPP
360 370 380 390 400
CQIEEENHEG EELKPPEQEV EIDRNVIQEE EKQAIPEFFE GRQTKTPERY
410 420 430 440 450
LKIRNYILDQ WEICKPKYLN KTSVRPGLKN CGDVNCIGRI HTYLELIGAI
460 470 480 490 500
NFGCEQAVYN RPQPLDKVRA ADRKDAEAAY QLAWRLQSMR TRRRRVRDPW
510 520 530 540 550
GNWCDAKDLE GQTFEHLSVE EMARRKEEEK CKPIKFSKAS KLPKSSLDPF
560 570 580 590 600
QLIPCNFFSE EKQEPFQVKV AAEALLIMNL HAHVSMAEVI GLLGGRYSEA
610 620 630 640 650
DKVLEVCAAE PCNSLSTGLQ CEMDPVSQTQ ASETLALRGY SVIGWYHSHP
660 670 680 690 700
AFDPNPSLRD IDTQAKYQSY FSRGGAKFIG MIVSPYNRSN PLPYSQITCL
710 720 730 740 750
VISEEVSPDG TYRLPYKFEV QQMLEEPQWE LVFEKTRWII EKYRLSNSSV
760 770 780 790 800
PMDRIFRRDS DLTCLQKLLE CLRKTLSKVA NCFIAEEFLT QIENLFLSNY
810
KSKEENGLAE EDSTKELFM
Note: No experimental confirmation available.
Length:819
Mass (Da):93,475
Last modified:May 2, 2006 - v2
Checksum:iB5A720311CD9C779
GO
Isoform 2 (identifier: Q69Z66-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     412-819: Missing.

Note: No experimental confirmation available.
Show »
Length:411
Mass (Da):46,702
Checksum:i7C2A5EF9CDD447DD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7D → G in BAE37628 (PubMed:16141072).Curated1
Sequence conflicti175Q → H in BAC31657 (PubMed:16141072).Curated1
Sequence conflicti221D → H in BAE37628 (PubMed:16141072).Curated1
Sequence conflicti265A → P in BAE37628 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_018212412 – 819Missing in isoform 2. 1 PublicationAdd BLAST408

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK043802 mRNA. Translation: BAC31657.1.
AK081684 mRNA. Translation: BAC38291.1.
AK164103 mRNA. Translation: BAE37628.1.
AL732611 Genomic DNA. Translation: CAM14751.1.
BC150946 mRNA. Translation: AAI50947.1.
BC151172 mRNA. Translation: AAI51173.1.
AK173300 mRNA. Translation: BAD32578.1.
CCDSiCCDS18363.1. [Q69Z66-1]
RefSeqiNP_796213.2. NM_177239.2. [Q69Z66-1]
UniGeneiMm.208868.
Mm.468748.

Genome annotation databases

EnsembliENSMUST00000075872; ENSMUSP00000075269; ENSMUSG00000062627. [Q69Z66-1]
GeneIDi320713.
KEGGimmu:320713.
UCSCiuc008tsn.1. mouse. [Q69Z66-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMYSM1_MOUSE
AccessioniPrimary (citable) accession number: Q69Z66
Secondary accession number(s): A2AJE3
, B9EKJ6, Q3TPV7, Q8BRP5, Q8C4N1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: May 2, 2006
Last modified: August 30, 2017
This is version 121 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families