Q69Z38 (PEAK1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pseudopodium-enriched atypical kinase 1
EC=2.7.10.2

Alternative name(s):
Sugen kinase 269
Tyrosine-protein kinase SgK269

Gene names

Name:	Peak1
Synonyms:	Kiaa2002, Sgk269

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1735 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Tyrosine kinase that may play a role in cell spreading and migration on fibronectin. May directly or indirectly affect phosphorylation levels of cytoskeleton-associated proteins MAPK1/ERK and PXN By similarity.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit structure	Interacts with BCAR1 and CRK By similarity.
Subcellular location	Cytoplasm › cytoskeleton. Cell junction › focal adhesion. Note: Colocalizes with actin. Ref.8
Post-translational modification	Autophosphorylated in vitro By similarity. Phosphorylated on tyrosine in a CSK-dependent manner in response to adhesion to fibronectin and to EGF stimulation. Ref.8
Sequence similarities	Belongs to the protein kinase superfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cell junction Cytoplasm Cytoskeleton
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell migration Inferred from sequence or structural similarity. Source: UniProtKB protein autophosphorylation Inferred from electronic annotation. Source: Compara substrate adhesion-dependent cell spreading Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	actin cytoskeleton Inferred from direct assay Ref.8. Source: UniProtKB cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW focal adhesion Inferred from direct assay Ref.8. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1735	1735	Pseudopodium-enriched atypical kinase 1		PRO_0000250590
Regions
	Domain	1302 – 1664	363	Protein kinase
	Nucleotide binding	1308 – 1316	9	ATP By similarity
	Compositional bias	145 – 148	4	Poly-Asn
	Compositional bias	775 – 872	98	Pro-rich
	Compositional bias	938 – 947	10	Poly-Glu
	Compositional bias	963 – 966	4	Poly-Pro
Sites
	Active site	1505	1	Proton acceptor By similarity
	Binding site	1348	1	ATP By similarity
Amino acid modifications
	Modified residue	282	1	Phosphoserine Ref.5 Ref.7
	Modified residue	632	1	Phosphotyrosine Ref.6
Experimental info
	Sequence conflict	560	1	K → R in BAC33490. Ref.2
	Sequence conflict	965 – 991	27	PPVQR…VFMYR → SSPAPSLVHRGQRRGQREAC YSLHVQV in BAC33490. Ref.2
	Sequence conflict	1026 – 1039	14	SSYLP…DKACS → GDPVKMSLNGELCD in BAD32606. Ref.3
	Sequence conflict	1085	1	L → S in BAD32606. Ref.3
	Sequence conflict	1534 – 1536	3	LAC → PTR in AAH26466. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q69Z38 [UniParc]. Last modified July 27, 2011. Version 4. Checksum: 99A629E9061A0BF0	FASTA	1,735	191,097
	10 20 30 40 50 60 MSACNTFTEH VWKPGECKNC FKPKSLHQLP PDSEKTPITH GSGKTNANHS NNHRVRSTGN 70 80 90 100 110 120 FRPPVAKKPT IAVKPTMMVA DGQSVCGELS IQEHCENKPV ILGWNQNKTS LSQKPLNNNS 130 140 150 160 170 180 EGDAEGFGSD PQQCANNDSA QKISNNNNGL TEVLKEIAGL EATPPVRGNE TNARETFLGR 190 200 210 220 230 240 INDCYKRSLE RKIPPSCMTG SMKDSQGKHV ILSGSAEVIS NEGGRFCYPE FSSGEESEED 250 260 270 280 290 300 VLFSNMEEEH ESWDESDEEL LAMEIRMRGQ PRFANFRANT LSPVRFFVSK KWNTIPLRNK 310 320 330 340 350 360 SLQRICAVDY DDSYDEILNG YEENSGVSYG QGSVQSTISS DCTSPGSSFT EESRSETASS 370 380 390 400 410 420 LSQKVCNGGI SPGNPGNSKD IAETESNFES PPGNNEEKDE SLTSKSSVKV PETHKAVLAL 430 440 450 460 470 480 RLQEKDGKIA VHTEKPESKA STDIAGQAVT ISLVPVEEQT KPYRVVNLEQ PLCKPYTVVD 490 500 510 520 530 540 VSAAMASEHL GRPKIKGSSS TPNSPVTSPA LTPGQINAHL KKSSAIRYQE VWTSSTSPRQ 550 560 570 580 590 600 KIPKIELSTG GPGPNVPPRK NCHKSAPTSP TATNISSKTI PVKSPNLSEI KFNSYNNAGM 610 620 630 640 650 660 PPFPIIIHDE PSYARSSKNA IKVPIVINPN AYDNLAIYKS FLGTSGELSV KEKTTSVISH 670 680 690 700 710 720 TYEEIETESK VSDSTPSKLT DCPQAKGFSN STERKRGSVA QKVQEFNNCL NRGQSSPQRS 730 740 750 760 770 780 YSSTHSSPAK IQRPTQEPAG KTEGAQGSQV PGSSSNSTRE KASAVLCQIV ASIQPPQTPP 790 800 810 820 830 840 EAPQSSPKAC SVEELYAVPP DADTTKSIPK NPPVRPKSLF TSQSSGEGEA HQTTESPTAK 850 860 870 880 890 900 IQKDPSTKPV TSPPSKLVTS AQSEPPPPFP PPRSTSSPYH ASNLLQRHFT NWTKPTSPTR 910 920 930 940 950 960 STEAESILHS EGSRRAADAK PKRWISFKSF FRRRKTDEEE EKEKEREKGK LVGLDGTVIH 970 980 990 1000 1010 1020 MLPPPPVQRH HWFTEAKGEA SEKPAIVFMY RCDPDQGHLS VDQSKAGAEK GRAEEVLLRN 1030 1040 1050 1060 1070 1080 SEEKKSSYLP SQIPDKACSR VTHEVAGELS PRDPRTPAGK QDGTSVTPTL PPPDLEREEE 1090 1100 1110 1120 1130 1140 KDDTLDPTDV SPCSATYSNL GQSRAAMIPP KHPRHPKGAV DDAIAFGEKT DQEGLNASQP 1150 1160 1170 1180 1190 1200 TPPPLPKKMI RANTEPISKD LQKAMESSLC VMANPTYDID PNWDASSAGS SISYELKGLD 1210 1220 1230 1240 1250 1260 VESYESLERP LHKERPVPSA ANSISSLATL SVKDRFSNSM ESLSSRRGLS YRQTRSIQKP 1270 1280 1290 1300 1310 1320 QRQALYRGLD NREEVVGKLR SLHTDALKRL AVKCEDLFMA GQKDQLRFGV DSWSDFRLTS 1330 1340 1350 1360 1370 1380 DKPCCEAGDA VYYTASYAKD PLSNYAVKIC KSKAKESQQY YHSLAVRQSL PVHFNIQQDC 1390 1400 1410 1420 1430 1440 GHFLAEVPSR LLPWEDPDAP EKAEDGTEDS EEEGKAETLG GNPEPCSETE PSQKENQRVT 1450 1460 1470 1480 1490 1500 NRKQRSHVVV ITREVPHLTV ADFVRDSLAH HGNSPDLYER QVCLLLLQLC SGLEHLKPYH 1510 1520 1530 1540 1550 1560 VTHCDLRLEN LLLVQHQPGG AAQGPSPADP CPTLACPTRL IVSNFSQAKQ KSHLVDPQIL 1570 1580 1590 1600 1610 1620 RDQSRLAPEI ITATQYKKCD EFQTGILIYE MLHLPNPFDE NPELKEKEYT RTDLPRIPLR 1630 1640 1650 1660 1670 1680 SPYSWGLQQL ASCLLNPNPS ERILISDAKG ILQCLLWGPR EDLFQIFTTS ATLAQKNALL 1690 1700 1710 1720 1730 QNWLDIKRTL LMIKFAEKSL DREGGISLED WLCAQYLAFA TTDSLSYIVK ILQYR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-991. Strain: C57BL/6J. Tissue: Cerebellum.
[3]	"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H. DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1026-1735. Tissue: Embryonic intestine.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1534-1735. Strain: FVB/N. Tissue: Mammary tumor.
[5]	"Comprehensive identification of phosphorylation sites in postsynaptic density preparations." Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L. Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-282, MASS SPECTROMETRY. Tissue: Brain.
[6]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-632, MASS SPECTROMETRY. Tissue: Brain.
[7]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-282, MASS SPECTROMETRY. Tissue: Macrophage.
[8]	"Pseudopodium-enriched atypical kinase 1 regulates the cytoskeleton and cancer progression." Wang Y., Kelber J.A., Tran Cao H.S., Cantin G.T., Lin R., Wang W., Kaushal S., Bristow J.M., Edgington T.S., Hoffman R.M., Bouvet M., Yates J.R. III, Klemke R.L. Proc. Natl. Acad. Sci. U.S.A. 107:10920-10925(2010) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION BY CSK.
[9]	Erratum Wang Y., Kelber J.A., Tran Cao H.S., Cantin G.T., Lin R., Wang W., Kaushal S., Bristow J.M., Edgington T.S., Hoffman R.M., Bouvet M., Yates J.R. III, Klemke R.L. Proc. Natl. Acad. Sci. U.S.A. 107:13556-13556(2010)
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC157474 Genomic DNA. No translation available. AC159819 Genomic DNA. No translation available. AC160935 Genomic DNA. No translation available. AK048912 mRNA. Translation: BAC33490.1. AK173328 mRNA. Translation: BAD32606.1. BC026466 mRNA. Translation: AAH26466.1.
IPI	IPI00136917.
RefSeq	NP_766512.2. NM_172924.3.
UniGene	Mm.490331.
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q69Z38.
Proteomic databases
PaxDb	Q69Z38.
PRIDE	Q69Z38.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000061552; ENSMUSP00000109901; ENSMUSG00000074305.
GeneID	244895.
KEGG	mmu:244895.
UCSC	uc009psz.2. mouse.
Organism-specific databases
MGI	MGI:2442366. C230081A13Rik.
Rouge	Search...
Phylogenomic databases
eggNOG	NOG115841.
GeneTree	ENSGT00460000041554.
HOVERGEN	HBG093946.
InParanoid	Q69Z38.
OMA	GNNDSAK.
OrthoDB	EOG4PVNXS.
Gene expression databases
Bgee	Q69Z38.
CleanEx	MM_C230081A13RIK.
Genevestigator	Q69Z38.
GermOnline	ENSMUSG00000066635. Mus musculus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR008266. Tyr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	386480.
SOURCE	Search...

Entry information

Entry name

PEAK1_MOUSE

Accession

Primary (citable) accession number: Q69Z38
Secondary accession number(s): E9QKY2, Q8BX56, Q8R365

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	July 27, 2011
Last modified:	March 6, 2013
This is version 71 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program