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Q69Z38

- PEAK1_MOUSE

UniProt

Q69Z38 - PEAK1_MOUSE

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Protein

Pseudopodium-enriched atypical kinase 1

Gene

Peak1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Tyrosine kinase that may play a role in cell spreading and migration on fibronectin. May directly or indirectly affect phosphorylation levels of cytoskeleton-associated proteins MAPK1/ERK and PXN (By similarity).By similarity

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei1348 – 13481ATPPROSITE-ProRule annotation
Active sitei1505 – 15051Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi1308 – 13169ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB

GO - Biological processi

  1. cell migration Source: UniProtKB
  2. peptidyl-tyrosine phosphorylation Source: GOC
  3. protein autophosphorylation Source: Ensembl
  4. substrate adhesion-dependent cell spreading Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pseudopodium-enriched atypical kinase 1 (EC:2.7.10.2)
Alternative name(s):
Sugen kinase 269
Tyrosine-protein kinase SgK269
Gene namesi
Name:Peak1
Synonyms:Kiaa2002, Sgk269
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:2442366. Peak1.

Subcellular locationi

Cytoplasmcytoskeleton 1 Publication. Cell junctionfocal adhesion 1 Publication
Note: Colocalizes with actin.

GO - Cellular componenti

  1. actin cytoskeleton Source: UniProtKB
  2. cytoplasm Source: UniProtKB-KW
  3. focal adhesion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 17351735Pseudopodium-enriched atypical kinase 1PRO_0000250590Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei282 – 2821Phosphoserine1 Publication
Modified residuei537 – 5371PhosphoserineBy similarity
Modified residuei584 – 5841PhosphoserineBy similarity
Modified residuei632 – 6321Phosphotyrosine2 Publications
Modified residuei638 – 6381Phosphotyrosine1 Publication

Post-translational modificationi

Autophosphorylated in vitro (By similarity). Phosphorylated on tyrosine in a CSK-dependent manner in response to adhesion to fibronectin and to EGF stimulation.By similarity4 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ69Z38.
PaxDbiQ69Z38.
PRIDEiQ69Z38.

PTM databases

PhosphoSiteiQ69Z38.

Expressioni

Gene expression databases

BgeeiQ69Z38.
CleanExiMM_C230081A13RIK.
GenevestigatoriQ69Z38.

Interactioni

Subunit structurei

Interacts with BCAR1 and CRK.By similarity

Protein-protein interaction databases

IntActiQ69Z38. 1 interaction.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1302 – 1664363Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi145 – 1484Poly-Asn
Compositional biasi775 – 87298Pro-richAdd
BLAST
Compositional biasi938 – 94710Poly-Glu
Compositional biasi963 – 9664Poly-Pro

Sequence similaritiesi

Belongs to the protein kinase superfamily.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG115841.
GeneTreeiENSGT00460000041554.
HOVERGENiHBG093946.
InParanoidiQ69Z38.
KOiK17538.
OMAiGRFCYPE.
OrthoDBiEOG7B8S2Z.
TreeFamiTF333340.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q69Z38-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSACNTFTEH VWKPGECKNC FKPKSLHQLP PDSEKTPITH GSGKTNANHS
60 70 80 90 100
NNHRVRSTGN FRPPVAKKPT IAVKPTMMVA DGQSVCGELS IQEHCENKPV
110 120 130 140 150
ILGWNQNKTS LSQKPLNNNS EGDAEGFGSD PQQCANNDSA QKISNNNNGL
160 170 180 190 200
TEVLKEIAGL EATPPVRGNE TNARETFLGR INDCYKRSLE RKIPPSCMTG
210 220 230 240 250
SMKDSQGKHV ILSGSAEVIS NEGGRFCYPE FSSGEESEED VLFSNMEEEH
260 270 280 290 300
ESWDESDEEL LAMEIRMRGQ PRFANFRANT LSPVRFFVSK KWNTIPLRNK
310 320 330 340 350
SLQRICAVDY DDSYDEILNG YEENSGVSYG QGSVQSTISS DCTSPGSSFT
360 370 380 390 400
EESRSETASS LSQKVCNGGI SPGNPGNSKD IAETESNFES PPGNNEEKDE
410 420 430 440 450
SLTSKSSVKV PETHKAVLAL RLQEKDGKIA VHTEKPESKA STDIAGQAVT
460 470 480 490 500
ISLVPVEEQT KPYRVVNLEQ PLCKPYTVVD VSAAMASEHL GRPKIKGSSS
510 520 530 540 550
TPNSPVTSPA LTPGQINAHL KKSSAIRYQE VWTSSTSPRQ KIPKIELSTG
560 570 580 590 600
GPGPNVPPRK NCHKSAPTSP TATNISSKTI PVKSPNLSEI KFNSYNNAGM
610 620 630 640 650
PPFPIIIHDE PSYARSSKNA IKVPIVINPN AYDNLAIYKS FLGTSGELSV
660 670 680 690 700
KEKTTSVISH TYEEIETESK VSDSTPSKLT DCPQAKGFSN STERKRGSVA
710 720 730 740 750
QKVQEFNNCL NRGQSSPQRS YSSTHSSPAK IQRPTQEPAG KTEGAQGSQV
760 770 780 790 800
PGSSSNSTRE KASAVLCQIV ASIQPPQTPP EAPQSSPKAC SVEELYAVPP
810 820 830 840 850
DADTTKSIPK NPPVRPKSLF TSQSSGEGEA HQTTESPTAK IQKDPSTKPV
860 870 880 890 900
TSPPSKLVTS AQSEPPPPFP PPRSTSSPYH ASNLLQRHFT NWTKPTSPTR
910 920 930 940 950
STEAESILHS EGSRRAADAK PKRWISFKSF FRRRKTDEEE EKEKEREKGK
960 970 980 990 1000
LVGLDGTVIH MLPPPPVQRH HWFTEAKGEA SEKPAIVFMY RCDPDQGHLS
1010 1020 1030 1040 1050
VDQSKAGAEK GRAEEVLLRN SEEKKSSYLP SQIPDKACSR VTHEVAGELS
1060 1070 1080 1090 1100
PRDPRTPAGK QDGTSVTPTL PPPDLEREEE KDDTLDPTDV SPCSATYSNL
1110 1120 1130 1140 1150
GQSRAAMIPP KHPRHPKGAV DDAIAFGEKT DQEGLNASQP TPPPLPKKMI
1160 1170 1180 1190 1200
RANTEPISKD LQKAMESSLC VMANPTYDID PNWDASSAGS SISYELKGLD
1210 1220 1230 1240 1250
VESYESLERP LHKERPVPSA ANSISSLATL SVKDRFSNSM ESLSSRRGLS
1260 1270 1280 1290 1300
YRQTRSIQKP QRQALYRGLD NREEVVGKLR SLHTDALKRL AVKCEDLFMA
1310 1320 1330 1340 1350
GQKDQLRFGV DSWSDFRLTS DKPCCEAGDA VYYTASYAKD PLSNYAVKIC
1360 1370 1380 1390 1400
KSKAKESQQY YHSLAVRQSL PVHFNIQQDC GHFLAEVPSR LLPWEDPDAP
1410 1420 1430 1440 1450
EKAEDGTEDS EEEGKAETLG GNPEPCSETE PSQKENQRVT NRKQRSHVVV
1460 1470 1480 1490 1500
ITREVPHLTV ADFVRDSLAH HGNSPDLYER QVCLLLLQLC SGLEHLKPYH
1510 1520 1530 1540 1550
VTHCDLRLEN LLLVQHQPGG AAQGPSPADP CPTLACPTRL IVSNFSQAKQ
1560 1570 1580 1590 1600
KSHLVDPQIL RDQSRLAPEI ITATQYKKCD EFQTGILIYE MLHLPNPFDE
1610 1620 1630 1640 1650
NPELKEKEYT RTDLPRIPLR SPYSWGLQQL ASCLLNPNPS ERILISDAKG
1660 1670 1680 1690 1700
ILQCLLWGPR EDLFQIFTTS ATLAQKNALL QNWLDIKRTL LMIKFAEKSL
1710 1720 1730
DREGGISLED WLCAQYLAFA TTDSLSYIVK ILQYR
Length:1,735
Mass (Da):191,097
Last modified:July 27, 2011 - v4
Checksum:i99A629E9061A0BF0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti560 – 5601K → R in BAC33490. (PubMed:16141072)Curated
Sequence conflicti965 – 99127PPVQR…VFMYR → SSPAPSLVHRGQRRGQREAC YSLHVQV in BAC33490. (PubMed:16141072)CuratedAdd
BLAST
Sequence conflicti1026 – 103914SSYLP…DKACS → GDPVKMSLNGELCD in BAD32606. (PubMed:15368895)CuratedAdd
BLAST
Sequence conflicti1085 – 10851L → S in BAD32606. (PubMed:15368895)Curated
Sequence conflicti1534 – 15363LAC → PTR in AAH26466. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC157474 Genomic DNA. No translation available.
AC159819 Genomic DNA. No translation available.
AC160935 Genomic DNA. No translation available.
AK048912 mRNA. Translation: BAC33490.1.
AK173328 mRNA. Translation: BAD32606.1.
BC026466 mRNA. Translation: AAH26466.1.
CCDSiCCDS52804.1.
RefSeqiNP_766512.2. NM_172924.3.
XP_006511230.1. XM_006511167.1.
XP_006511231.1. XM_006511168.1.
XP_006511232.1. XM_006511169.1.
UniGeneiMm.490331.

Genome annotation databases

EnsembliENSMUST00000061552; ENSMUSP00000109901; ENSMUSG00000074305.
GeneIDi244895.
KEGGimmu:244895.
UCSCiuc009psz.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC157474 Genomic DNA. No translation available.
AC159819 Genomic DNA. No translation available.
AC160935 Genomic DNA. No translation available.
AK048912 mRNA. Translation: BAC33490.1 .
AK173328 mRNA. Translation: BAD32606.1 .
BC026466 mRNA. Translation: AAH26466.1 .
CCDSi CCDS52804.1.
RefSeqi NP_766512.2. NM_172924.3.
XP_006511230.1. XM_006511167.1.
XP_006511231.1. XM_006511168.1.
XP_006511232.1. XM_006511169.1.
UniGenei Mm.490331.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q69Z38. 1 interaction.

PTM databases

PhosphoSitei Q69Z38.

Proteomic databases

MaxQBi Q69Z38.
PaxDbi Q69Z38.
PRIDEi Q69Z38.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000061552 ; ENSMUSP00000109901 ; ENSMUSG00000074305 .
GeneIDi 244895.
KEGGi mmu:244895.
UCSCi uc009psz.2. mouse.

Organism-specific databases

CTDi 79834.
MGIi MGI:2442366. Peak1.
Rougei Search...

Phylogenomic databases

eggNOGi NOG115841.
GeneTreei ENSGT00460000041554.
HOVERGENi HBG093946.
InParanoidi Q69Z38.
KOi K17538.
OMAi GRFCYPE.
OrthoDBi EOG7B8S2Z.
TreeFami TF333340.

Miscellaneous databases

NextBioi 386480.
PROi Q69Z38.
SOURCEi Search...

Gene expression databases

Bgeei Q69Z38.
CleanExi MM_C230081A13RIK.
Genevestigatori Q69Z38.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008266. Tyr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 2 hits.
PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-991.
    Strain: C57BL/6J.
    Tissue: Cerebellum.
  3. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
    DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1026-1735.
    Tissue: Embryonic intestine.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1534-1735.
    Strain: FVB/N.
    Tissue: Mammary tumor.
  5. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
    Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
    Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-632 AND TYR-638, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-632, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  7. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-282, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION BY CSK.

Entry informationi

Entry nameiPEAK1_MOUSE
AccessioniPrimary (citable) accession number: Q69Z38
Secondary accession number(s): E9QKY2, Q8BX56, Q8R365
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 84 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3