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Q69Z38

- PEAK1_MOUSE

UniProt

Q69Z38 - PEAK1_MOUSE

Protein

Pseudopodium-enriched atypical kinase 1

Gene

Peak1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 4 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Tyrosine kinase that may play a role in cell spreading and migration on fibronectin. May directly or indirectly affect phosphorylation levels of cytoskeleton-associated proteins MAPK1/ERK and PXN By similarity.By similarity

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei1348 – 13481ATPPROSITE-ProRule annotation
    Active sitei1505 – 15051Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi1308 – 13169ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB

    GO - Biological processi

    1. cell migration Source: UniProtKB
    2. peptidyl-tyrosine phosphorylation Source: GOC
    3. protein autophosphorylation Source: Ensembl
    4. substrate adhesion-dependent cell spreading Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pseudopodium-enriched atypical kinase 1 (EC:2.7.10.2)
    Alternative name(s):
    Sugen kinase 269
    Tyrosine-protein kinase SgK269
    Gene namesi
    Name:Peak1
    Synonyms:Kiaa2002, Sgk269
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:2442366. Peak1.

    Subcellular locationi

    Cytoplasmcytoskeleton 1 Publication. Cell junctionfocal adhesion 1 Publication
    Note: Colocalizes with actin.

    GO - Cellular componenti

    1. actin cytoskeleton Source: UniProtKB
    2. cytoplasm Source: UniProtKB-KW
    3. focal adhesion Source: UniProtKB

    Keywords - Cellular componenti

    Cell junction, Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 17351735Pseudopodium-enriched atypical kinase 1PRO_0000250590Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei282 – 2821Phosphoserine2 Publications
    Modified residuei537 – 5371PhosphoserineBy similarity
    Modified residuei584 – 5841PhosphoserineBy similarity
    Modified residuei632 – 6321Phosphotyrosine3 Publications
    Modified residuei638 – 6381Phosphotyrosine2 Publications

    Post-translational modificationi

    Autophosphorylated in vitro By similarity. Phosphorylated on tyrosine in a CSK-dependent manner in response to adhesion to fibronectin and to EGF stimulation.By similarity4 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ69Z38.
    PaxDbiQ69Z38.
    PRIDEiQ69Z38.

    PTM databases

    PhosphoSiteiQ69Z38.

    Expressioni

    Gene expression databases

    BgeeiQ69Z38.
    CleanExiMM_C230081A13RIK.
    GenevestigatoriQ69Z38.

    Interactioni

    Subunit structurei

    Interacts with BCAR1 and CRK.By similarity

    Protein-protein interaction databases

    IntActiQ69Z38. 1 interaction.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1302 – 1664363Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi145 – 1484Poly-Asn
    Compositional biasi775 – 87298Pro-richAdd
    BLAST
    Compositional biasi938 – 94710Poly-Glu
    Compositional biasi963 – 9664Poly-Pro

    Sequence similaritiesi

    Belongs to the protein kinase superfamily.Curated
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG115841.
    GeneTreeiENSGT00460000041554.
    HOVERGENiHBG093946.
    InParanoidiQ69Z38.
    KOiK17538.
    OMAiGRFCYPE.
    OrthoDBiEOG7B8S2Z.
    TreeFamiTF333340.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR008266. Tyr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 2 hits.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q69Z38-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSACNTFTEH VWKPGECKNC FKPKSLHQLP PDSEKTPITH GSGKTNANHS     50
    NNHRVRSTGN FRPPVAKKPT IAVKPTMMVA DGQSVCGELS IQEHCENKPV 100
    ILGWNQNKTS LSQKPLNNNS EGDAEGFGSD PQQCANNDSA QKISNNNNGL 150
    TEVLKEIAGL EATPPVRGNE TNARETFLGR INDCYKRSLE RKIPPSCMTG 200
    SMKDSQGKHV ILSGSAEVIS NEGGRFCYPE FSSGEESEED VLFSNMEEEH 250
    ESWDESDEEL LAMEIRMRGQ PRFANFRANT LSPVRFFVSK KWNTIPLRNK 300
    SLQRICAVDY DDSYDEILNG YEENSGVSYG QGSVQSTISS DCTSPGSSFT 350
    EESRSETASS LSQKVCNGGI SPGNPGNSKD IAETESNFES PPGNNEEKDE 400
    SLTSKSSVKV PETHKAVLAL RLQEKDGKIA VHTEKPESKA STDIAGQAVT 450
    ISLVPVEEQT KPYRVVNLEQ PLCKPYTVVD VSAAMASEHL GRPKIKGSSS 500
    TPNSPVTSPA LTPGQINAHL KKSSAIRYQE VWTSSTSPRQ KIPKIELSTG 550
    GPGPNVPPRK NCHKSAPTSP TATNISSKTI PVKSPNLSEI KFNSYNNAGM 600
    PPFPIIIHDE PSYARSSKNA IKVPIVINPN AYDNLAIYKS FLGTSGELSV 650
    KEKTTSVISH TYEEIETESK VSDSTPSKLT DCPQAKGFSN STERKRGSVA 700
    QKVQEFNNCL NRGQSSPQRS YSSTHSSPAK IQRPTQEPAG KTEGAQGSQV 750
    PGSSSNSTRE KASAVLCQIV ASIQPPQTPP EAPQSSPKAC SVEELYAVPP 800
    DADTTKSIPK NPPVRPKSLF TSQSSGEGEA HQTTESPTAK IQKDPSTKPV 850
    TSPPSKLVTS AQSEPPPPFP PPRSTSSPYH ASNLLQRHFT NWTKPTSPTR 900
    STEAESILHS EGSRRAADAK PKRWISFKSF FRRRKTDEEE EKEKEREKGK 950
    LVGLDGTVIH MLPPPPVQRH HWFTEAKGEA SEKPAIVFMY RCDPDQGHLS 1000
    VDQSKAGAEK GRAEEVLLRN SEEKKSSYLP SQIPDKACSR VTHEVAGELS 1050
    PRDPRTPAGK QDGTSVTPTL PPPDLEREEE KDDTLDPTDV SPCSATYSNL 1100
    GQSRAAMIPP KHPRHPKGAV DDAIAFGEKT DQEGLNASQP TPPPLPKKMI 1150
    RANTEPISKD LQKAMESSLC VMANPTYDID PNWDASSAGS SISYELKGLD 1200
    VESYESLERP LHKERPVPSA ANSISSLATL SVKDRFSNSM ESLSSRRGLS 1250
    YRQTRSIQKP QRQALYRGLD NREEVVGKLR SLHTDALKRL AVKCEDLFMA 1300
    GQKDQLRFGV DSWSDFRLTS DKPCCEAGDA VYYTASYAKD PLSNYAVKIC 1350
    KSKAKESQQY YHSLAVRQSL PVHFNIQQDC GHFLAEVPSR LLPWEDPDAP 1400
    EKAEDGTEDS EEEGKAETLG GNPEPCSETE PSQKENQRVT NRKQRSHVVV 1450
    ITREVPHLTV ADFVRDSLAH HGNSPDLYER QVCLLLLQLC SGLEHLKPYH 1500
    VTHCDLRLEN LLLVQHQPGG AAQGPSPADP CPTLACPTRL IVSNFSQAKQ 1550
    KSHLVDPQIL RDQSRLAPEI ITATQYKKCD EFQTGILIYE MLHLPNPFDE 1600
    NPELKEKEYT RTDLPRIPLR SPYSWGLQQL ASCLLNPNPS ERILISDAKG 1650
    ILQCLLWGPR EDLFQIFTTS ATLAQKNALL QNWLDIKRTL LMIKFAEKSL 1700
    DREGGISLED WLCAQYLAFA TTDSLSYIVK ILQYR 1735
    Length:1,735
    Mass (Da):191,097
    Last modified:July 27, 2011 - v4
    Checksum:i99A629E9061A0BF0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti560 – 5601K → R in BAC33490. (PubMed:16141072)Curated
    Sequence conflicti965 – 99127PPVQR…VFMYR → SSPAPSLVHRGQRRGQREAC YSLHVQV in BAC33490. (PubMed:16141072)CuratedAdd
    BLAST
    Sequence conflicti1026 – 103914SSYLP…DKACS → GDPVKMSLNGELCD in BAD32606. (PubMed:15368895)CuratedAdd
    BLAST
    Sequence conflicti1085 – 10851L → S in BAD32606. (PubMed:15368895)Curated
    Sequence conflicti1534 – 15363LAC → PTR in AAH26466. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC157474 Genomic DNA. No translation available.
    AC159819 Genomic DNA. No translation available.
    AC160935 Genomic DNA. No translation available.
    AK048912 mRNA. Translation: BAC33490.1.
    AK173328 mRNA. Translation: BAD32606.1.
    BC026466 mRNA. Translation: AAH26466.1.
    CCDSiCCDS52804.1.
    RefSeqiNP_766512.2. NM_172924.3.
    XP_006511230.1. XM_006511167.1.
    XP_006511231.1. XM_006511168.1.
    XP_006511232.1. XM_006511169.1.
    UniGeneiMm.490331.

    Genome annotation databases

    EnsembliENSMUST00000061552; ENSMUSP00000109901; ENSMUSG00000074305.
    GeneIDi244895.
    KEGGimmu:244895.
    UCSCiuc009psz.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC157474 Genomic DNA. No translation available.
    AC159819 Genomic DNA. No translation available.
    AC160935 Genomic DNA. No translation available.
    AK048912 mRNA. Translation: BAC33490.1 .
    AK173328 mRNA. Translation: BAD32606.1 .
    BC026466 mRNA. Translation: AAH26466.1 .
    CCDSi CCDS52804.1.
    RefSeqi NP_766512.2. NM_172924.3.
    XP_006511230.1. XM_006511167.1.
    XP_006511231.1. XM_006511168.1.
    XP_006511232.1. XM_006511169.1.
    UniGenei Mm.490331.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q69Z38. 1 interaction.

    PTM databases

    PhosphoSitei Q69Z38.

    Proteomic databases

    MaxQBi Q69Z38.
    PaxDbi Q69Z38.
    PRIDEi Q69Z38.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000061552 ; ENSMUSP00000109901 ; ENSMUSG00000074305 .
    GeneIDi 244895.
    KEGGi mmu:244895.
    UCSCi uc009psz.2. mouse.

    Organism-specific databases

    CTDi 79834.
    MGIi MGI:2442366. Peak1.
    Rougei Search...

    Phylogenomic databases

    eggNOGi NOG115841.
    GeneTreei ENSGT00460000041554.
    HOVERGENi HBG093946.
    InParanoidi Q69Z38.
    KOi K17538.
    OMAi GRFCYPE.
    OrthoDBi EOG7B8S2Z.
    TreeFami TF333340.

    Miscellaneous databases

    NextBioi 386480.
    PROi Q69Z38.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q69Z38.
    CleanExi MM_C230081A13RIK.
    Genevestigatori Q69Z38.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR008266. Tyr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 2 hits.
    PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-991.
      Strain: C57BL/6J.
      Tissue: Cerebellum.
    3. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
      DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1026-1735.
      Tissue: Embryonic intestine.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1534-1735.
      Strain: FVB/N.
      Tissue: Mammary tumor.
    5. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
      Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
      Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-632 AND TYR-638, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
      Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
      J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-632, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    7. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-282, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION BY CSK.

    Entry informationi

    Entry nameiPEAK1_MOUSE
    AccessioniPrimary (citable) accession number: Q69Z38
    Secondary accession number(s): E9QKY2, Q8BX56, Q8R365
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2006
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 83 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3