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Q69Z38 (PEAK1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pseudopodium-enriched atypical kinase 1

EC=2.7.10.2
Alternative name(s):
Sugen kinase 269
Tyrosine-protein kinase SgK269
Gene names
Name:Peak1
Synonyms:Kiaa2002, Sgk269
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1735 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Tyrosine kinase that may play a role in cell spreading and migration on fibronectin. May directly or indirectly affect phosphorylation levels of cytoskeleton-associated proteins MAPK1/ERK and PXN By similarity.

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure

Interacts with BCAR1 and CRK By similarity.

Subcellular location

Cytoplasmcytoskeleton. Cell junctionfocal adhesion. Note: Colocalizes with actin. Ref.8

Post-translational modification

Autophosphorylated in vitro By similarity. Phosphorylated on tyrosine in a CSK-dependent manner in response to adhesion to fibronectin and to EGF stimulation. Ref.8

Sequence similarities

Belongs to the protein kinase superfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 17351735Pseudopodium-enriched atypical kinase 1
PRO_0000250590

Regions

Domain1302 – 1664363Protein kinase
Nucleotide binding1308 – 13169ATP By similarity
Compositional bias145 – 1484Poly-Asn
Compositional bias775 – 87298Pro-rich
Compositional bias938 – 94710Poly-Glu
Compositional bias963 – 9664Poly-Pro

Sites

Active site15051Proton acceptor By similarity
Binding site13481ATP By similarity

Amino acid modifications

Modified residue2821Phosphoserine Ref.7
Modified residue5371Phosphoserine By similarity
Modified residue5841Phosphoserine By similarity
Modified residue6321Phosphotyrosine Ref.5 Ref.6
Modified residue6381Phosphotyrosine Ref.5

Experimental info

Sequence conflict5601K → R in BAC33490. Ref.2
Sequence conflict965 – 99127PPVQR…VFMYR → SSPAPSLVHRGQRRGQREAC YSLHVQV in BAC33490. Ref.2
Sequence conflict1026 – 103914SSYLP…DKACS → GDPVKMSLNGELCD in BAD32606. Ref.3
Sequence conflict10851L → S in BAD32606. Ref.3
Sequence conflict1534 – 15363LAC → PTR in AAH26466. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q69Z38 [UniParc].

Last modified July 27, 2011. Version 4.
Checksum: 99A629E9061A0BF0

FASTA1,735191,097
        10         20         30         40         50         60 
MSACNTFTEH VWKPGECKNC FKPKSLHQLP PDSEKTPITH GSGKTNANHS NNHRVRSTGN 

        70         80         90        100        110        120 
FRPPVAKKPT IAVKPTMMVA DGQSVCGELS IQEHCENKPV ILGWNQNKTS LSQKPLNNNS 

       130        140        150        160        170        180 
EGDAEGFGSD PQQCANNDSA QKISNNNNGL TEVLKEIAGL EATPPVRGNE TNARETFLGR 

       190        200        210        220        230        240 
INDCYKRSLE RKIPPSCMTG SMKDSQGKHV ILSGSAEVIS NEGGRFCYPE FSSGEESEED 

       250        260        270        280        290        300 
VLFSNMEEEH ESWDESDEEL LAMEIRMRGQ PRFANFRANT LSPVRFFVSK KWNTIPLRNK 

       310        320        330        340        350        360 
SLQRICAVDY DDSYDEILNG YEENSGVSYG QGSVQSTISS DCTSPGSSFT EESRSETASS 

       370        380        390        400        410        420 
LSQKVCNGGI SPGNPGNSKD IAETESNFES PPGNNEEKDE SLTSKSSVKV PETHKAVLAL 

       430        440        450        460        470        480 
RLQEKDGKIA VHTEKPESKA STDIAGQAVT ISLVPVEEQT KPYRVVNLEQ PLCKPYTVVD 

       490        500        510        520        530        540 
VSAAMASEHL GRPKIKGSSS TPNSPVTSPA LTPGQINAHL KKSSAIRYQE VWTSSTSPRQ 

       550        560        570        580        590        600 
KIPKIELSTG GPGPNVPPRK NCHKSAPTSP TATNISSKTI PVKSPNLSEI KFNSYNNAGM 

       610        620        630        640        650        660 
PPFPIIIHDE PSYARSSKNA IKVPIVINPN AYDNLAIYKS FLGTSGELSV KEKTTSVISH 

       670        680        690        700        710        720 
TYEEIETESK VSDSTPSKLT DCPQAKGFSN STERKRGSVA QKVQEFNNCL NRGQSSPQRS 

       730        740        750        760        770        780 
YSSTHSSPAK IQRPTQEPAG KTEGAQGSQV PGSSSNSTRE KASAVLCQIV ASIQPPQTPP 

       790        800        810        820        830        840 
EAPQSSPKAC SVEELYAVPP DADTTKSIPK NPPVRPKSLF TSQSSGEGEA HQTTESPTAK 

       850        860        870        880        890        900 
IQKDPSTKPV TSPPSKLVTS AQSEPPPPFP PPRSTSSPYH ASNLLQRHFT NWTKPTSPTR 

       910        920        930        940        950        960 
STEAESILHS EGSRRAADAK PKRWISFKSF FRRRKTDEEE EKEKEREKGK LVGLDGTVIH 

       970        980        990       1000       1010       1020 
MLPPPPVQRH HWFTEAKGEA SEKPAIVFMY RCDPDQGHLS VDQSKAGAEK GRAEEVLLRN 

      1030       1040       1050       1060       1070       1080 
SEEKKSSYLP SQIPDKACSR VTHEVAGELS PRDPRTPAGK QDGTSVTPTL PPPDLEREEE 

      1090       1100       1110       1120       1130       1140 
KDDTLDPTDV SPCSATYSNL GQSRAAMIPP KHPRHPKGAV DDAIAFGEKT DQEGLNASQP 

      1150       1160       1170       1180       1190       1200 
TPPPLPKKMI RANTEPISKD LQKAMESSLC VMANPTYDID PNWDASSAGS SISYELKGLD 

      1210       1220       1230       1240       1250       1260 
VESYESLERP LHKERPVPSA ANSISSLATL SVKDRFSNSM ESLSSRRGLS YRQTRSIQKP 

      1270       1280       1290       1300       1310       1320 
QRQALYRGLD NREEVVGKLR SLHTDALKRL AVKCEDLFMA GQKDQLRFGV DSWSDFRLTS 

      1330       1340       1350       1360       1370       1380 
DKPCCEAGDA VYYTASYAKD PLSNYAVKIC KSKAKESQQY YHSLAVRQSL PVHFNIQQDC 

      1390       1400       1410       1420       1430       1440 
GHFLAEVPSR LLPWEDPDAP EKAEDGTEDS EEEGKAETLG GNPEPCSETE PSQKENQRVT 

      1450       1460       1470       1480       1490       1500 
NRKQRSHVVV ITREVPHLTV ADFVRDSLAH HGNSPDLYER QVCLLLLQLC SGLEHLKPYH 

      1510       1520       1530       1540       1550       1560 
VTHCDLRLEN LLLVQHQPGG AAQGPSPADP CPTLACPTRL IVSNFSQAKQ KSHLVDPQIL 

      1570       1580       1590       1600       1610       1620 
RDQSRLAPEI ITATQYKKCD EFQTGILIYE MLHLPNPFDE NPELKEKEYT RTDLPRIPLR 

      1630       1640       1650       1660       1670       1680 
SPYSWGLQQL ASCLLNPNPS ERILISDAKG ILQCLLWGPR EDLFQIFTTS ATLAQKNALL 

      1690       1700       1710       1720       1730 
QNWLDIKRTL LMIKFAEKSL DREGGISLED WLCAQYLAFA TTDSLSYIVK ILQYR 

« Hide

References

« Hide 'large scale' references
[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-991.
Strain: C57BL/6J.
Tissue: Cerebellum.
[3]"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1026-1735.
Tissue: Embryonic intestine.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1534-1735.
Strain: FVB/N.
Tissue: Mammary tumor.
[5]"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-632 AND TYR-638, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-632, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
[7]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-282, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Pseudopodium-enriched atypical kinase 1 regulates the cytoskeleton and cancer progression."
Wang Y., Kelber J.A., Tran Cao H.S., Cantin G.T., Lin R., Wang W., Kaushal S., Bristow J.M., Edgington T.S., Hoffman R.M., Bouvet M., Yates J.R. III, Klemke R.L.
Proc. Natl. Acad. Sci. U.S.A. 107:10920-10925(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION BY CSK.
[9]Erratum
Wang Y., Kelber J.A., Tran Cao H.S., Cantin G.T., Lin R., Wang W., Kaushal S., Bristow J.M., Edgington T.S., Hoffman R.M., Bouvet M., Yates J.R. III, Klemke R.L.
Proc. Natl. Acad. Sci. U.S.A. 107:13556-13556(2010)
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC157474 Genomic DNA. No translation available.
AC159819 Genomic DNA. No translation available.
AC160935 Genomic DNA. No translation available.
AK048912 mRNA. Translation: BAC33490.1.
AK173328 mRNA. Translation: BAD32606.1.
BC026466 mRNA. Translation: AAH26466.1.
CCDSCCDS52804.1.
RefSeqNP_766512.2. NM_172924.3.
XP_006511230.1. XM_006511167.1.
XP_006511231.1. XM_006511168.1.
XP_006511232.1. XM_006511169.1.
UniGeneMm.490331.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ69Z38. 1 interaction.

PTM databases

PhosphoSiteQ69Z38.

Proteomic databases

MaxQBQ69Z38.
PaxDbQ69Z38.
PRIDEQ69Z38.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000061552; ENSMUSP00000109901; ENSMUSG00000074305.
GeneID244895.
KEGGmmu:244895.
UCSCuc009psz.2. mouse.

Organism-specific databases

CTD79834.
MGIMGI:2442366. Peak1.
RougeSearch...

Phylogenomic databases

eggNOGNOG115841.
GeneTreeENSGT00460000041554.
HOVERGENHBG093946.
InParanoidQ69Z38.
KOK17538.
OMAGRFCYPE.
OrthoDBEOG7B8S2Z.
TreeFamTF333340.

Gene expression databases

BgeeQ69Z38.
CleanExMM_C230081A13RIK.
GenevestigatorQ69Z38.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 2 hits.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio386480.
PROQ69Z38.
SOURCESearch...

Entry information

Entry namePEAK1_MOUSE
AccessionPrimary (citable) accession number: Q69Z38
Secondary accession number(s): E9QKY2, Q8BX56, Q8R365
Entry history
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 82 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot