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Protein

A disintegrin and metalloproteinase with thrombospondin motifs 16

Gene

Adamts16

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi247 – 2471Zinc; in inhibited formBy similarity
Metal bindingi431 – 4311Zinc; catalyticPROSITE-ProRule annotation
Active sitei432 – 4321PROSITE-ProRule annotation
Metal bindingi435 – 4351Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi441 – 4411Zinc; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM12.026.

Names & Taxonomyi

Protein namesi
Recommended name:
A disintegrin and metalloproteinase with thrombospondin motifs 16 (EC:3.4.24.-)
Short name:
ADAM-TS 16
Short name:
ADAM-TS16
Short name:
ADAMTS-16
Gene namesi
Name:Adamts16
Synonyms:Kiaa2029
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:2429637. Adamts16.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Propeptidei21 – 277257By similarityPRO_0000029196Add
BLAST
Chaini278 – 1222945A disintegrin and metalloproteinase with thrombospondin motifs 16PRO_0000029197Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi154 – 1541N-linked (GlcNAc...)Sequence analysis
Glycosylationi190 – 1901N-linked (GlcNAc...)Sequence analysis
Glycosylationi308 – 3081N-linked (GlcNAc...)Sequence analysis
Disulfide bondi364 ↔ 415By similarity
Disulfide bondi390 ↔ 397By similarity
Disulfide bondi409 ↔ 488By similarity
Disulfide bondi448 ↔ 472By similarity
Disulfide bondi516 ↔ 541By similarity
Disulfide bondi527 ↔ 548By similarity
Disulfide bondi536 ↔ 567By similarity
Disulfide bondi561 ↔ 572By similarity
Disulfide bondi596 ↔ 633By similarity
Disulfide bondi600 ↔ 638By similarity
Disulfide bondi611 ↔ 623By similarity
Glycosylationi739 – 7391N-linked (GlcNAc...)Sequence analysis
Glycosylationi778 – 7781N-linked (GlcNAc...)Sequence analysis
Glycosylationi825 – 8251N-linked (GlcNAc...)Sequence analysis
Glycosylationi833 – 8331N-linked (GlcNAc...)Sequence analysis
Glycosylationi903 – 9031N-linked (GlcNAc...)Sequence analysis
Glycosylationi933 – 9331N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

The precursor is cleaved by a furin endopeptidase.By similarity
Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity).By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ69Z28.
PRIDEiQ69Z28.

PTM databases

PhosphoSiteiQ69Z28.

Expressioni

Gene expression databases

BgeeiQ69Z28.
ExpressionAtlasiQ69Z28. baseline and differential.
GenevisibleiQ69Z28. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000079041.

Structurei

3D structure databases

ProteinModelPortaliQ69Z28.
SMRiQ69Z28. Positions 290-801.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini288 – 493206Peptidase M12BPROSITE-ProRule annotationAdd
BLAST
Domaini494 – 58390DisintegrinAdd
BLAST
Domaini584 – 63956TSP type-1 1PROSITE-ProRule annotationAdd
BLAST
Domaini872 – 92049TSP type-1 2PROSITE-ProRule annotationAdd
BLAST
Domaini925 – 98561TSP type-1 3PROSITE-ProRule annotationAdd
BLAST
Domaini986 – 104661TSP type-1 4PROSITE-ProRule annotationAdd
BLAST
Domaini1049 – 111365TSP type-1 5PROSITE-ProRule annotationAdd
BLAST
Domaini1125 – 117955TSP type-1 6PROSITE-ProRule annotationAdd
BLAST
Domaini1184 – 122138PLACPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni745 – 871127SpacerAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi245 – 2539Cysteine switchBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi640 – 744105Cys-richAdd
BLAST

Domaini

The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.By similarity
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Contains 1 disintegrin domain.Curated
Contains 1 peptidase M12B domain.PROSITE-ProRule annotation
Contains 1 PLAC domain.PROSITE-ProRule annotation
Contains 6 TSP type-1 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG3538. Eukaryota.
ENOG410XPKZ. LUCA.
GeneTreeiENSGT00760000118880.
HOGENOMiHOG000004800.
HOVERGENiHBG004315.
InParanoidiQ69Z28.
KOiK08630.
OMAiEDQDLCK.
OrthoDBiEOG73FQKT.
PhylomeDBiQ69Z28.
TreeFamiTF313537.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
InterProiIPR010294. ADAM_spacer1.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR013273. Peptidase_M12B_ADAM-TS.
IPR002870. Peptidase_M12B_N.
IPR010909. PLAC.
IPR000884. TSP1_rpt.
[Graphical view]
PfamiPF05986. ADAM_spacer1. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF08686. PLAC. 1 hit.
PF01421. Reprolysin. 1 hit.
PF00090. TSP_1. 5 hits.
[Graphical view]
PRINTSiPR01857. ADAMTSFAMILY.
SMARTiSM00209. TSP1. 6 hits.
[Graphical view]
SUPFAMiSSF82895. SSF82895. 5 hits.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS50900. PLAC. 1 hit.
PS50092. TSP1. 5 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q69Z28-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESRGCAALW VLLLAQVSEQ QTPACALGLA AAASGSPEDP QPPPFSGSSW
60 70 80 90 100
LETGEYDLVS AYEVDHRGDY VSHDIMHYQR RRRRRAVTQP GGDALHLRLK
110 120 130 140 150
GPRHDLHLDL KAASNLMAPG FMVQTLGKGG TKSVQMFPPE ENCFYQGSLR
160 170 180 190 200
SQGNSSVALS TCQGLLGMIR TKDTDYFLKP LPPHLTSKLN RSAQGDSPSH
210 220 230 240 250
VLYKRSTERQ APRENEVLMI TRKRDLARPH LHHDNFHLGP SQKQHFCGRR
260 270 280 290 300
KKYMPQPPND DLYILPDEYK PSSRHKRSLL KSHRNEELNV ETLVVVDRKM
310 320 330 340 350
MQSHGHENIT TYVLTILNMV SALFKDGTIG GNINIVIVGL ILLEDEQPGL
360 370 380 390 400
AISHHADHTL TSFCQWQSGL MGKDGTRHDH AILLTGLDIC SWKNEPCDTL
410 420 430 440 450
GFAPISGMCS KYRSCTVNED SGLGLAFTIA HESGHNFGMV HDGEGNMCKK
460 470 480 490 500
SEGNIMSPTL AGRNGVFSWS SCSRQYLHKF LSTAQAICLA DQPKPVKEYK
510 520 530 540 550
YPEKLPGQLY DANTQCKWQF GEKAKLCMLD FRKDICKALW CHRIGRKCET
560 570 580 590 600
KFMPAAEGTL CGQDMWCRGG QCVKYGDEGP KPTHGHWSDW SPWSPCSRTC
610 620 630 640 650
GGGISHRDRL CTNPRPSHGG KFCQGSTRTL KLCNSQRCPL DSVDFRAAQC
660 670 680 690 700
AEYNSKRFRG WLYKWKPYTQ LEDQDLCKLY CIAEGFDFFF SLSNKVKDGT
710 720 730 740 750
PCSEDSRNVC IDGMCERVGC DNVLGSDATE DSCGVCKGNN SDCVTHRGLY
760 770 780 790 800
SKHHSTNQYY HMVTIPSGAR SIHIYETNIS TSYISVRNSL KRYYLNGHWS
810 820 830 840 850
VDWPGRYKFS GATFNYKRSY KEPENLTSPG PTNETLIVEL LFQGRNPGVA
860 870 880 890 900
WEFSLPRSGA KKTPAAQPSY SWAIVRSECS VSCGGGKMNS KAGCYRDLKV
910 920 930 940 950
PVNASFCNPK TRPVTGLVPC KVSPCPSSWS VGNWSVCSRT CGGGTQSRPV
960 970 980 990 1000
RCTRRAHYRD ESIPASLCPQ PEPPIHQACN SQSCPPAWST GPWAECSRTC
1010 1020 1030 1040 1050
GKGWRKRTVA CKSTNPSARA QLLHDTACTS EPKPRTHEIC LLKRCHKHKK
1060 1070 1080 1090 1100
LQWLVSAWSQ CSVTCQGGTQ QRVLRCAEKY ISGKYRELAS KKCLHLPKPD
1110 1120 1130 1140 1150
LELERACGLI PCPKHPPFDA SGSPRGSWFA SPWSQCTASC GGGVQRRTVQ
1160 1170 1180 1190 1200
CLLRGQPASD CFLHEKPETS SACNTHFCPI AEKRGTFCKD LFHWCYLVPQ
1210 1220
HGMCGHRFYS KQCCNTCSKS NL
Length:1,222
Mass (Da):136,282
Last modified:August 16, 2005 - v2
Checksum:i3A95FB40A8F15083
GO
Isoform 2 (identifier: Q69Z28-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     886-900: GKMNSKAGCYRDLKV → DRQCQTGRGHLEISS
     901-1222: Missing.

Note: No experimental confirmation available.
Show »
Length:900
Mass (Da):100,680
Checksum:i590BE2B0E73740FF
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei886 – 90015GKMNS…RDLKV → DRQCQTGRGHLEISS in isoform 2. 1 PublicationVSP_014991Add
BLAST
Alternative sequencei901 – 1222322Missing in isoform 2. 1 PublicationVSP_014992Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK173338 mRNA. Translation: BAD32616.1.
BC034739 mRNA. Translation: AAH34739.1.
CCDSiCCDS36723.1. [Q69Z28-1]
RefSeqiNP_742050.2. NM_172053.2. [Q69Z28-1]
UniGeneiMm.115970.

Genome annotation databases

EnsembliENSMUST00000080145; ENSMUSP00000079041; ENSMUSG00000049538. [Q69Z28-1]
ENSMUST00000109694; ENSMUSP00000105316; ENSMUSG00000049538. [Q69Z28-2]
GeneIDi271127.
KEGGimmu:271127.
UCSCiuc007rcz.1. mouse. [Q69Z28-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK173338 mRNA. Translation: BAD32616.1.
BC034739 mRNA. Translation: AAH34739.1.
CCDSiCCDS36723.1. [Q69Z28-1]
RefSeqiNP_742050.2. NM_172053.2. [Q69Z28-1]
UniGeneiMm.115970.

3D structure databases

ProteinModelPortaliQ69Z28.
SMRiQ69Z28. Positions 290-801.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000079041.

Protein family/group databases

MEROPSiM12.026.

PTM databases

PhosphoSiteiQ69Z28.

Proteomic databases

PaxDbiQ69Z28.
PRIDEiQ69Z28.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000080145; ENSMUSP00000079041; ENSMUSG00000049538. [Q69Z28-1]
ENSMUST00000109694; ENSMUSP00000105316; ENSMUSG00000049538. [Q69Z28-2]
GeneIDi271127.
KEGGimmu:271127.
UCSCiuc007rcz.1. mouse. [Q69Z28-1]

Organism-specific databases

CTDi170690.
MGIiMGI:2429637. Adamts16.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG3538. Eukaryota.
ENOG410XPKZ. LUCA.
GeneTreeiENSGT00760000118880.
HOGENOMiHOG000004800.
HOVERGENiHBG004315.
InParanoidiQ69Z28.
KOiK08630.
OMAiEDQDLCK.
OrthoDBiEOG73FQKT.
PhylomeDBiQ69Z28.
TreeFamiTF313537.

Miscellaneous databases

NextBioi393419.
PROiQ69Z28.
SOURCEiSearch...

Gene expression databases

BgeeiQ69Z28.
ExpressionAtlasiQ69Z28. baseline and differential.
GenevisibleiQ69Z28. MM.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
InterProiIPR010294. ADAM_spacer1.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR013273. Peptidase_M12B_ADAM-TS.
IPR002870. Peptidase_M12B_N.
IPR010909. PLAC.
IPR000884. TSP1_rpt.
[Graphical view]
PfamiPF05986. ADAM_spacer1. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF08686. PLAC. 1 hit.
PF01421. Reprolysin. 1 hit.
PF00090. TSP_1. 5 hits.
[Graphical view]
PRINTSiPR01857. ADAMTSFAMILY.
SMARTiSM00209. TSP1. 6 hits.
[Graphical view]
SUPFAMiSSF82895. SSF82895. 5 hits.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS50900. PLAC. 1 hit.
PS50092. TSP1. 5 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
    DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Embryonic intestine.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Mammary gland.

Entry informationi

Entry nameiATS16_MOUSE
AccessioniPrimary (citable) accession number: Q69Z28
Secondary accession number(s): Q8K206
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: August 16, 2005
Last modified: November 11, 2015
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.