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Protein

A disintegrin and metalloproteinase with thrombospondin motifs 16

Gene

Adamts16

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi247Zinc; in inhibited formBy similarity1
Metal bindingi431Zinc; catalyticPROSITE-ProRule annotation1
Active sitei432PROSITE-ProRule annotation1
Metal bindingi435Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi441Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-5173214 O-glycosylation of TSR domain-containing proteins

Protein family/group databases

MEROPSiM12.026

Names & Taxonomyi

Protein namesi
Recommended name:
A disintegrin and metalloproteinase with thrombospondin motifs 16 (EC:3.4.24.-)
Short name:
ADAM-TS 16
Short name:
ADAM-TS16
Short name:
ADAMTS-16
Gene namesi
Name:Adamts16
Synonyms:Kiaa2029
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:2429637 Adamts16

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
PropeptideiPRO_000002919621 – 277By similarityAdd BLAST257
ChainiPRO_0000029197278 – 1222A disintegrin and metalloproteinase with thrombospondin motifs 16Add BLAST945

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi154N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi190N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi308N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi364 ↔ 415By similarity
Disulfide bondi390 ↔ 397By similarity
Disulfide bondi409 ↔ 488By similarity
Disulfide bondi448 ↔ 472By similarity
Disulfide bondi516 ↔ 541By similarity
Disulfide bondi527 ↔ 548By similarity
Disulfide bondi536 ↔ 567By similarity
Disulfide bondi561 ↔ 572By similarity
Disulfide bondi596 ↔ 633By similarity
Disulfide bondi600 ↔ 638By similarity
Disulfide bondi611 ↔ 623By similarity
Glycosylationi739N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi778N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi825N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi833N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi903N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi933N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

The precursor is cleaved by a furin endopeptidase.By similarity
Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X2-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity).By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ69Z28
PRIDEiQ69Z28

PTM databases

iPTMnetiQ69Z28
PhosphoSitePlusiQ69Z28

Expressioni

Gene expression databases

BgeeiENSMUSG00000049538
ExpressionAtlasiQ69Z28 baseline and differential
GenevisibleiQ69Z28 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000079041

Structurei

3D structure databases

ProteinModelPortaliQ69Z28
SMRiQ69Z28
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini288 – 493Peptidase M12BPROSITE-ProRule annotationAdd BLAST206
Domaini494 – 583DisintegrinAdd BLAST90
Domaini584 – 639TSP type-1 1PROSITE-ProRule annotationAdd BLAST56
Domaini872 – 920TSP type-1 2PROSITE-ProRule annotationAdd BLAST49
Domaini925 – 985TSP type-1 3PROSITE-ProRule annotationAdd BLAST61
Domaini986 – 1046TSP type-1 4PROSITE-ProRule annotationAdd BLAST61
Domaini1049 – 1113TSP type-1 5PROSITE-ProRule annotationAdd BLAST65
Domaini1125 – 1179TSP type-1 6PROSITE-ProRule annotationAdd BLAST55
Domaini1184 – 1221PLACPROSITE-ProRule annotationAdd BLAST38

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni745 – 871SpacerAdd BLAST127

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi245 – 253Cysteine switchBy similarity9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi640 – 744Cys-richAdd BLAST105

Domaini

The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.By similarity
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG3538 Eukaryota
ENOG410XPKZ LUCA
GeneTreeiENSGT00900000140815
HOGENOMiHOG000004800
HOVERGENiHBG004315
InParanoidiQ69Z28
KOiK08630
OMAiKKLQWLV
OrthoDBiEOG091G14M8
PhylomeDBiQ69Z28
TreeFamiTF313537

Family and domain databases

Gene3Di2.20.100.10, 5 hits
3.40.390.10, 1 hit
InterProiView protein in InterPro
IPR010294 ADAM_spacer1
IPR024079 MetalloPept_cat_dom_sf
IPR001590 Peptidase_M12B
IPR002870 Peptidase_M12B_N
IPR010909 PLAC
IPR000884 TSP1_rpt
IPR036383 TSP1_rpt_sf
PfamiView protein in Pfam
PF05986 ADAM_spacer1, 1 hit
PF01562 Pep_M12B_propep, 1 hit
PF08686 PLAC, 1 hit
PF01421 Reprolysin, 1 hit
PF00090 TSP_1, 5 hits
SMARTiView protein in SMART
SM00209 TSP1, 6 hits
SUPFAMiSSF82895 SSF82895, 5 hits
PROSITEiView protein in PROSITE
PS50215 ADAM_MEPRO, 1 hit
PS50900 PLAC, 1 hit
PS50092 TSP1, 5 hits

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q69Z28-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESRGCAALW VLLLAQVSEQ QTPACALGLA AAASGSPEDP QPPPFSGSSW
60 70 80 90 100
LETGEYDLVS AYEVDHRGDY VSHDIMHYQR RRRRRAVTQP GGDALHLRLK
110 120 130 140 150
GPRHDLHLDL KAASNLMAPG FMVQTLGKGG TKSVQMFPPE ENCFYQGSLR
160 170 180 190 200
SQGNSSVALS TCQGLLGMIR TKDTDYFLKP LPPHLTSKLN RSAQGDSPSH
210 220 230 240 250
VLYKRSTERQ APRENEVLMI TRKRDLARPH LHHDNFHLGP SQKQHFCGRR
260 270 280 290 300
KKYMPQPPND DLYILPDEYK PSSRHKRSLL KSHRNEELNV ETLVVVDRKM
310 320 330 340 350
MQSHGHENIT TYVLTILNMV SALFKDGTIG GNINIVIVGL ILLEDEQPGL
360 370 380 390 400
AISHHADHTL TSFCQWQSGL MGKDGTRHDH AILLTGLDIC SWKNEPCDTL
410 420 430 440 450
GFAPISGMCS KYRSCTVNED SGLGLAFTIA HESGHNFGMV HDGEGNMCKK
460 470 480 490 500
SEGNIMSPTL AGRNGVFSWS SCSRQYLHKF LSTAQAICLA DQPKPVKEYK
510 520 530 540 550
YPEKLPGQLY DANTQCKWQF GEKAKLCMLD FRKDICKALW CHRIGRKCET
560 570 580 590 600
KFMPAAEGTL CGQDMWCRGG QCVKYGDEGP KPTHGHWSDW SPWSPCSRTC
610 620 630 640 650
GGGISHRDRL CTNPRPSHGG KFCQGSTRTL KLCNSQRCPL DSVDFRAAQC
660 670 680 690 700
AEYNSKRFRG WLYKWKPYTQ LEDQDLCKLY CIAEGFDFFF SLSNKVKDGT
710 720 730 740 750
PCSEDSRNVC IDGMCERVGC DNVLGSDATE DSCGVCKGNN SDCVTHRGLY
760 770 780 790 800
SKHHSTNQYY HMVTIPSGAR SIHIYETNIS TSYISVRNSL KRYYLNGHWS
810 820 830 840 850
VDWPGRYKFS GATFNYKRSY KEPENLTSPG PTNETLIVEL LFQGRNPGVA
860 870 880 890 900
WEFSLPRSGA KKTPAAQPSY SWAIVRSECS VSCGGGKMNS KAGCYRDLKV
910 920 930 940 950
PVNASFCNPK TRPVTGLVPC KVSPCPSSWS VGNWSVCSRT CGGGTQSRPV
960 970 980 990 1000
RCTRRAHYRD ESIPASLCPQ PEPPIHQACN SQSCPPAWST GPWAECSRTC
1010 1020 1030 1040 1050
GKGWRKRTVA CKSTNPSARA QLLHDTACTS EPKPRTHEIC LLKRCHKHKK
1060 1070 1080 1090 1100
LQWLVSAWSQ CSVTCQGGTQ QRVLRCAEKY ISGKYRELAS KKCLHLPKPD
1110 1120 1130 1140 1150
LELERACGLI PCPKHPPFDA SGSPRGSWFA SPWSQCTASC GGGVQRRTVQ
1160 1170 1180 1190 1200
CLLRGQPASD CFLHEKPETS SACNTHFCPI AEKRGTFCKD LFHWCYLVPQ
1210 1220
HGMCGHRFYS KQCCNTCSKS NL
Length:1,222
Mass (Da):136,282
Last modified:August 16, 2005 - v2
Checksum:i3A95FB40A8F15083
GO
Isoform 2 (identifier: Q69Z28-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     886-900: GKMNSKAGCYRDLKV → DRQCQTGRGHLEISS
     901-1222: Missing.

Note: No experimental confirmation available.
Show »
Length:900
Mass (Da):100,680
Checksum:i590BE2B0E73740FF
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_014991886 – 900GKMNS…RDLKV → DRQCQTGRGHLEISS in isoform 2. 1 PublicationAdd BLAST15
Alternative sequenceiVSP_014992901 – 1222Missing in isoform 2. 1 PublicationAdd BLAST322

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK173338 mRNA Translation: BAD32616.1
BC034739 mRNA Translation: AAH34739.1
CCDSiCCDS36723.1 [Q69Z28-1]
RefSeqiNP_742050.2, NM_172053.3 [Q69Z28-1]
UniGeneiMm.115970

Genome annotation databases

EnsembliENSMUST00000080145; ENSMUSP00000079041; ENSMUSG00000049538 [Q69Z28-1]
ENSMUST00000109694; ENSMUSP00000105316; ENSMUSG00000049538 [Q69Z28-2]
GeneIDi271127
KEGGimmu:271127
UCSCiuc007rcz.1 mouse [Q69Z28-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiATS16_MOUSE
AccessioniPrimary (citable) accession number: Q69Z28
Secondary accession number(s): Q8K206
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: August 16, 2005
Last modified: March 28, 2018
This is version 109 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health