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Q69Z26 (CNTN4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Contactin-4
Alternative name(s):
Brain-derived immunoglobulin superfamily protein 2
Short name=BIG-2
Gene names
Name:Cntn4
Synonyms:Kiaa3024
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1026 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity. May be involved in synaptogenesis. Ref.5

Subunit structure

Interacts with PTPRG. Ref.6

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor. Secreted By similarity.

Tissue specificity

Expressed in the region of developing olfactory neurons. Isoform 2 is expressed in mature sensory cells of the vomeronasal neuroepithelium and at lower level in olfactory neuroepithelium. Ref.5

Developmental stage

Expressed from E14 at a time when developing axons reach the olfactory bulb. Ref.5

Sequence similarities

Belongs to the immunoglobulin superfamily. Contactin family.

Contains 4 fibronectin type-III domains.

Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q69Z26-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q69Z26-2)

Also known as: BIG-2A;

The sequence of this isoform differs from the canonical sequence as follows:
     698-705: LPEVTPAN → QNTGDQRG
     706-1026: Missing.
Isoform 3 (identifier: Q69Z26-3)

The sequence of this isoform differs from the canonical sequence as follows:
     496-498: DPT → GNG
     499-1026: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 1000982Contactin-4
PRO_0000014713
Propeptide1001 – 102626Removed in mature form Potential
PRO_0000014714

Regions

Domain32 – 11786Ig-like C2-type 1
Domain122 – 20786Ig-like C2-type 2
Domain225 – 31187Ig-like C2-type 3
Domain316 – 40085Ig-like C2-type 4
Domain406 – 49388Ig-like C2-type 5
Domain497 – 58690Ig-like C2-type 6
Domain599 – 69799Fibronectin type-III 1
Domain702 – 79998Fibronectin type-III 2
Domain804 – 89996Fibronectin type-III 3
Domain900 – 99596Fibronectin type-III 4

Amino acid modifications

Lipidation10001GPI-anchor amidated serine Potential
Glycosylation651N-linked (GlcNAc...) Ref.6
Glycosylation901N-linked (GlcNAc...) Ref.6
Glycosylation1911N-linked (GlcNAc...) Ref.6
Glycosylation3701N-linked (GlcNAc...) Ref.6
Glycosylation3751N-linked (GlcNAc...) Potential
Glycosylation4661N-linked (GlcNAc...) Potential
Glycosylation7051N-linked (GlcNAc...) Potential
Glycosylation7641N-linked (GlcNAc...) Potential
Glycosylation8581N-linked (GlcNAc...) Potential
Glycosylation8931N-linked (GlcNAc...) Potential
Glycosylation9111N-linked (GlcNAc...) Potential
Glycosylation9291N-linked (GlcNAc...) Potential
Glycosylation9541N-linked (GlcNAc...) Potential
Disulfide bond50 ↔ 100 Ref.6
Disulfide bond144 ↔ 194 Ref.6
Disulfide bond247 ↔ 295 Ref.6
Disulfide bond337 ↔ 384 Ref.6
Disulfide bond429 ↔ 477 By similarity
Disulfide bond519 ↔ 576 By similarity

Natural variations

Alternative sequence496 – 4983DPT → GNG in isoform 3.
VSP_011963
Alternative sequence499 – 1026528Missing in isoform 3.
VSP_011964
Alternative sequence698 – 7058LPEVTPAN → QNTGDQRG in isoform 2.
VSP_011965
Alternative sequence706 – 1026321Missing in isoform 2.
VSP_011966

Experimental info

Sequence conflict641I → L in CAA67504. Ref.1
Sequence conflict641I → L in AAI15767. Ref.3
Sequence conflict1991T → S in CAA67504. Ref.1
Sequence conflict202 – 2032NH → HP in CAA67504. Ref.1
Sequence conflict2241E → D in CAA67504. Ref.1
Sequence conflict3061A → V in BAC29365. Ref.2
Sequence conflict3391A → G in CAA67504. Ref.1
Sequence conflict3431P → A in CAA67504. Ref.1
Sequence conflict3501L → V in CAA67504. Ref.1
Sequence conflict3701N → T in CAA67504. Ref.1
Sequence conflict373 – 3742IV → KG in CAA67504. Ref.1
Sequence conflict3771S → Q in CAA67504. Ref.1
Sequence conflict382 – 3865YQCVA → SQRVG in CAA67504. Ref.1
Sequence conflict4931I → V in CAA67504. Ref.1
Sequence conflict4931I → V in AAI15767. Ref.3
Sequence conflict6861S → G in BAC29365. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 23, 2004. Version 2.
Checksum: 34AC35CE432F03AC

FASTA1,026113,490
        10         20         30         40         50         60 
MRLPWELLVL QSFMLCLADD YTLHGPVFVQ EPSHVMFPLD SEEKKVKLSC EVKGNPKPHI 

        70         80         90        100        110        120 
RWKINGTDVD IGMDFRYSVV DGSLLINNPN KTQDAGTYQC IATNSFGTIV SREAKLQFAY 

       130        140        150        160        170        180 
LENFKTRTRS TVSVRRGQGM VLLCGPPPHS GELSYAWIFN EYPSYQDNRR FVSQETGNLY 

       190        200        210        220        230        240 
IAKVEKSDVG NYTCVVTNTV TNHKVLGPPT PLILRNDGVM GEYEPKIEVQ FPETVPAEKG 

       250        260        270        280        290        300 
TTVKLECFAL GNPVPTILWR RADGKPIARK ARRHKSNGIL EIPNFQQEDA GSYECVAENS 

       310        320        330        340        350        360 
RGKNVAKGQL TFYAQPNWVQ IINDIHVAME ESVFWECKAN GRPKPTYRWL KNGDPLLTRD 

       370        380        390        400        410        420 
RIQIEQGTLN ITIVNLSDAG MYQCVAENKH GVIFSSAELS VIAESPDFSR TLLKRVTLVK 

       430        440        450        460        470        480 
VGGEVVIECK PKASPRPVYT WRKGREILRE NERITISEDG NLRIINVTKS DAGSYTCIAT 

       490        500        510        520        530        540 
NHFGTASSTG NVIVKDPTKV MVPPSSMDVT VGESIVLPCQ VTHDHSLDIV FTWTFNGHLI 

       550        560        570        580        590        600 
DFDKDGDHFE RVGGQDSAGD LMIRNIQLKH AGKYVCMVQT SVDKLSVAAD LIVRGPPGPP 

       610        620        630        640        650        660 
EAVTIDEITD TTAQLSWRPG PDNHSPITMY VIQARTPFSV GWQAVNTVPD LVDGKTFTAT 

       670        680        690        700        710        720 
VVGLNPWVEY EFRTVAANVI GIGEPSRPSE KRRTEEALPE VTPANVSGGG GSKSELVITW 

       730        740        750        760        770        780 
ETVPEELQNG RGFGYVVAFR PHGKMIWMLT VLASADASRY VFRNESVRPF SPFEVKVGVF 

       790        800        810        820        830        840 
NNKGEGPFSP TTLVYSAEEE PTKPPASIFA RSLSATDIEV FWASPIGKNR GRIQGYEVKY 

       850        860        870        880        890        900 
WRHDDKEENA KKIRTVGNQT STKITNLKGS ALYHLSVKAY NSAGTGPSSA TVNVTTRKPP 

       910        920        930        940        950        960 
PSQPPGNIIW NSSDSKIILN WDQVKALDNE SEVKGYKVLY RWNRQSSTSV IETNKTSVEL 

       970        980        990       1000       1010       1020 
SLPFDEDYII EIKPFSDGGD GSSSEQIRIP KISNSYARGS GASTSNACTL SAISTIMISL 


TARSSL 

« Hide

Isoform 2 (BIG-2A) [UniParc].

Checksum: 1AA5A089B21237D7
Show »

FASTA70578,425
Isoform 3 [UniParc].

Checksum: 32B0AFB2EF8804F0
Show »

FASTA49855,719

References

« Hide 'large scale' references
[1]"A novel splice variant of the cell adhesion molecule BIG-2 is expressed in the olfactory and vomeronasal neuroepithelia."
Mimmack M.L., Saito H., Evans G., Bresler M., Keverne E.B., Emson P.C.
Brain Res. Mol. Brain Res. 47:345-350(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
Tissue: Nasal epithelium.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Strain: C57BL/6J.
Tissue: Brain cortex and Cerebellum.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[4]"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 129-1026 (ISOFORM 1).
Tissue: Fetal brain.
[5]"Expression of olfactory receptors, G-proteins and AxCAMs during the development and maturation of olfactory sensory neurons in the mouse."
Saito H., Mimmack M., Kishimoto J., Keverne E.B., Emson P.C.
Brain Res. Dev. Brain Res. 110:69-81(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[6]"The protein tyrosine phosphatases PTPRZ and PTPRG bind to distinct members of the contactin family of neural recognition molecules."
Bouyain S., Watkins D.J.
Proc. Natl. Acad. Sci. U.S.A. 107:2443-2448(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 25-404 IN COMPLEX WITH PTPRG, INTERACTION WITH PTPRG, GLYCOSYLATION AT ASN-65; ASN-90; ASN-191 AND ASN-370, DISULFIDE BONDS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X99043 Genomic DNA. Translation: CAA67504.1.
AK036262 mRNA. Translation: BAC29365.1.
AK043507 mRNA. Translation: BAC31563.1.
BC115766 mRNA. Translation: AAI15767.1.
AK173340 mRNA. Translation: BAD32618.1.
RefSeqNP_001103219.1. NM_001109749.1.
NP_001103221.1. NM_001109751.1.
NP_766592.2. NM_173004.3.
XP_006506256.1. XM_006506193.1.
XP_006506257.1. XM_006506194.1.
XP_006506258.1. XM_006506195.1.
UniGeneMm.321683.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3JXAX-ray2.40A/B25-404[»]
3KLDX-ray2.00A25-404[»]
ProteinModelPortalQ69Z26.
SMRQ69Z26. Positions 24-995.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4997439.
STRING10090.ENSMUSP00000108889.

Proteomic databases

PaxDbQ69Z26.
PRIDEQ69Z26.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000089208; ENSMUSP00000086616; ENSMUSG00000064293. [Q69Z26-1]
ENSMUST00000113258; ENSMUSP00000108883; ENSMUSG00000064293. [Q69Z26-3]
ENSMUST00000113260; ENSMUSP00000108885; ENSMUSG00000064293. [Q69Z26-2]
ENSMUST00000113261; ENSMUSP00000108886; ENSMUSG00000064293. [Q69Z26-2]
ENSMUST00000113264; ENSMUSP00000108889; ENSMUSG00000064293. [Q69Z26-1]
GeneID269784.
KEGGmmu:269784.
UCSCuc009dcr.2. mouse. [Q69Z26-2]
uc009dcu.1. mouse. [Q69Z26-3]
uc009dcv.2. mouse. [Q69Z26-1]

Organism-specific databases

CTD152330.
MGIMGI:1095737. Cntn4.
RougeSearch...

Phylogenomic databases

eggNOGNOG293951.
GeneTreeENSGT00740000115273.
HOGENOMHOG000059617.
HOVERGENHBG051047.
InParanoidQ69Z26.
KOK06762.
OMAFMLCLAD.
OrthoDBEOG7J17Z5.
PhylomeDBQ69Z26.
TreeFamTF351103.

Gene expression databases

ArrayExpressQ69Z26.
BgeeQ69Z26.
CleanExMM_CNTN4.
GenevestigatorQ69Z26.

Family and domain databases

Gene3D2.60.40.10. 10 hits.
InterProIPR003961. Fibronectin_type3.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PfamPF00041. fn3. 2 hits.
PF07679. I-set. 5 hits.
[Graphical view]
SMARTSM00060. FN3. 4 hits.
SM00409. IG. 2 hits.
SM00408. IGc2. 4 hits.
[Graphical view]
SUPFAMSSF49265. SSF49265. 2 hits.
PROSITEPS50853. FN3. 4 hits.
PS50835. IG_LIKE. 6 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCNTN4. mouse.
NextBio393030.
PROQ69Z26.
SOURCESearch...

Entry information

Entry nameCNTN4_MOUSE
AccessionPrimary (citable) accession number: Q69Z26
Secondary accession number(s): Q14BL8, Q8BRT6, Q8CBD3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: November 23, 2004
Last modified: April 16, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot