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Protein

Contactin-4

Gene

Cntn4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity. May be involved in synaptogenesis.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Contactin-4
Alternative name(s):
Brain-derived immunoglobulin superfamily protein 2
Short name:
BIG-2
Gene namesi
Name:Cntn4
Synonyms:Kiaa3024
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1095737. Cntn4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000001471319 – 1000Contactin-4Add BLAST982
PropeptideiPRO_00000147141001 – 1026Removed in mature formSequence analysisAdd BLAST26

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi50 ↔ 100PROSITE-ProRule annotation1 Publication
Glycosylationi65N-linked (GlcNAc...)1 Publication1
Glycosylationi90N-linked (GlcNAc...)1 Publication1
Disulfide bondi144 ↔ 194PROSITE-ProRule annotation1 Publication
Glycosylationi191N-linked (GlcNAc...)1 Publication1
Disulfide bondi247 ↔ 295PROSITE-ProRule annotation1 Publication
Disulfide bondi337 ↔ 384PROSITE-ProRule annotation1 Publication
Glycosylationi370N-linked (GlcNAc...)1 Publication1
Glycosylationi375N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi429 ↔ 477PROSITE-ProRule annotation
Glycosylationi466N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi519 ↔ 576PROSITE-ProRule annotation
Glycosylationi705N-linked (GlcNAc...)Sequence analysis1
Glycosylationi764N-linked (GlcNAc...)Sequence analysis1
Glycosylationi858N-linked (GlcNAc...)Sequence analysis1
Glycosylationi893N-linked (GlcNAc...)Sequence analysis1
Glycosylationi911N-linked (GlcNAc...)Sequence analysis1
Glycosylationi929N-linked (GlcNAc...)Sequence analysis1
Glycosylationi954N-linked (GlcNAc...)Sequence analysis1
Lipidationi1000GPI-anchor amidated serineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ69Z26.
PeptideAtlasiQ69Z26.
PRIDEiQ69Z26.

PTM databases

iPTMnetiQ69Z26.
PhosphoSitePlusiQ69Z26.

Expressioni

Tissue specificityi

Expressed in the region of developing olfactory neurons. Isoform 2 is expressed in mature sensory cells of the vomeronasal neuroepithelium and at lower level in olfactory neuroepithelium.1 Publication

Developmental stagei

Expressed from E14 at a time when developing axons reach the olfactory bulb.1 Publication

Gene expression databases

BgeeiENSMUSG00000064293.
CleanExiMM_CNTN4.
ExpressionAtlasiQ69Z26. baseline and differential.
GenevisibleiQ69Z26. MM.

Interactioni

Subunit structurei

Interacts with PTPRG.1 Publication

Protein-protein interaction databases

MINTiMINT-4997439.
STRINGi10090.ENSMUSP00000086616.

Structurei

Secondary structure

11026
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi601 – 608Combined sources8
Beta strandi613 – 618Combined sources6
Beta strandi629 – 635Combined sources7
Beta strandi637 – 641Combined sources5
Beta strandi646 – 652Combined sources7
Beta strandi658 – 663Combined sources6
Beta strandi669 – 677Combined sources9
Beta strandi715 – 719Combined sources5
Helixi725 – 727Combined sources3
Beta strandi730 – 732Combined sources3
Beta strandi734 – 741Combined sources8
Beta strandi748 – 752Combined sources5
Beta strandi759 – 763Combined sources5
Beta strandi772 – 781Combined sources10
Beta strandi784 – 788Combined sources5
Beta strandi792 – 795Combined sources4
Beta strandi806 – 812Combined sources7
Beta strandi814 – 816Combined sources3
Beta strandi818 – 823Combined sources6
Beta strandi835 – 842Combined sources8
Helixi847 – 849Combined sources3
Beta strandi851 – 854Combined sources4
Beta strandi861 – 864Combined sources4
Beta strandi872 – 881Combined sources10
Beta strandi884 – 888Combined sources5
Beta strandi892 – 895Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JXAX-ray2.40A/B25-404[»]
3KLDX-ray2.00A25-404[»]
5E4SX-ray2.50A596-898[»]
ProteinModelPortaliQ69Z26.
SMRiQ69Z26.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 117Ig-like C2-type 1Add BLAST86
Domaini122 – 207Ig-like C2-type 2Add BLAST86
Domaini225 – 311Ig-like C2-type 3Add BLAST87
Domaini316 – 400Ig-like C2-type 4Add BLAST85
Domaini406 – 493Ig-like C2-type 5Add BLAST88
Domaini497 – 586Ig-like C2-type 6Add BLAST90
Domaini599 – 697Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST99
Domaini702 – 799Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST98
Domaini804 – 899Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST96
Domaini900 – 995Fibronectin type-III 4PROSITE-ProRule annotationAdd BLAST96

Sequence similaritiesi

Contains 4 fibronectin type-III domains.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal

Phylogenomic databases

eggNOGiKOG3513. Eukaryota.
ENOG410XSVG. LUCA.
GeneTreeiENSGT00760000118840.
HOGENOMiHOG000059617.
HOVERGENiHBG051047.
InParanoidiQ69Z26.
KOiK06762.
OMAiYWKKEHK.
OrthoDBiEOG091G00X7.
PhylomeDBiQ69Z26.
TreeFamiTF351103.

Family and domain databases

CDDicd00063. FN3. 4 hits.
Gene3Di2.60.40.10. 10 hits.
InterProiIPR033007. Contactin-4.
IPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PANTHERiPTHR10489:SF568. PTHR10489:SF568. 1 hit.
PfamiPF00041. fn3. 2 hits.
PF07679. I-set. 2 hits.
[Graphical view]
SMARTiSM00060. FN3. 4 hits.
SM00409. IG. 6 hits.
SM00408. IGc2. 5 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 6 hits.
SSF49265. SSF49265. 2 hits.
PROSITEiPS50853. FN3. 4 hits.
PS50835. IG_LIKE. 6 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q69Z26-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRLPWELLVL QSFMLCLADD YTLHGPVFVQ EPSHVMFPLD SEEKKVKLSC
60 70 80 90 100
EVKGNPKPHI RWKINGTDVD IGMDFRYSVV DGSLLINNPN KTQDAGTYQC
110 120 130 140 150
IATNSFGTIV SREAKLQFAY LENFKTRTRS TVSVRRGQGM VLLCGPPPHS
160 170 180 190 200
GELSYAWIFN EYPSYQDNRR FVSQETGNLY IAKVEKSDVG NYTCVVTNTV
210 220 230 240 250
TNHKVLGPPT PLILRNDGVM GEYEPKIEVQ FPETVPAEKG TTVKLECFAL
260 270 280 290 300
GNPVPTILWR RADGKPIARK ARRHKSNGIL EIPNFQQEDA GSYECVAENS
310 320 330 340 350
RGKNVAKGQL TFYAQPNWVQ IINDIHVAME ESVFWECKAN GRPKPTYRWL
360 370 380 390 400
KNGDPLLTRD RIQIEQGTLN ITIVNLSDAG MYQCVAENKH GVIFSSAELS
410 420 430 440 450
VIAESPDFSR TLLKRVTLVK VGGEVVIECK PKASPRPVYT WRKGREILRE
460 470 480 490 500
NERITISEDG NLRIINVTKS DAGSYTCIAT NHFGTASSTG NVIVKDPTKV
510 520 530 540 550
MVPPSSMDVT VGESIVLPCQ VTHDHSLDIV FTWTFNGHLI DFDKDGDHFE
560 570 580 590 600
RVGGQDSAGD LMIRNIQLKH AGKYVCMVQT SVDKLSVAAD LIVRGPPGPP
610 620 630 640 650
EAVTIDEITD TTAQLSWRPG PDNHSPITMY VIQARTPFSV GWQAVNTVPD
660 670 680 690 700
LVDGKTFTAT VVGLNPWVEY EFRTVAANVI GIGEPSRPSE KRRTEEALPE
710 720 730 740 750
VTPANVSGGG GSKSELVITW ETVPEELQNG RGFGYVVAFR PHGKMIWMLT
760 770 780 790 800
VLASADASRY VFRNESVRPF SPFEVKVGVF NNKGEGPFSP TTLVYSAEEE
810 820 830 840 850
PTKPPASIFA RSLSATDIEV FWASPIGKNR GRIQGYEVKY WRHDDKEENA
860 870 880 890 900
KKIRTVGNQT STKITNLKGS ALYHLSVKAY NSAGTGPSSA TVNVTTRKPP
910 920 930 940 950
PSQPPGNIIW NSSDSKIILN WDQVKALDNE SEVKGYKVLY RWNRQSSTSV
960 970 980 990 1000
IETNKTSVEL SLPFDEDYII EIKPFSDGGD GSSSEQIRIP KISNSYARGS
1010 1020
GASTSNACTL SAISTIMISL TARSSL
Length:1,026
Mass (Da):113,490
Last modified:November 23, 2004 - v2
Checksum:i34AC35CE432F03AC
GO
Isoform 2 (identifier: Q69Z26-2) [UniParc]FASTAAdd to basket
Also known as: BIG-2A

The sequence of this isoform differs from the canonical sequence as follows:
     698-705: LPEVTPAN → QNTGDQRG
     706-1026: Missing.

Show »
Length:705
Mass (Da):78,425
Checksum:i1AA5A089B21237D7
GO
Isoform 3 (identifier: Q69Z26-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     496-498: DPT → GNG
     499-1026: Missing.

Note: No experimental confirmation available.
Show »
Length:498
Mass (Da):55,719
Checksum:i32B0AFB2EF8804F0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti64I → L in CAA67504 (PubMed:9221934).Curated1
Sequence conflicti64I → L in AAI15767 (PubMed:15489334).Curated1
Sequence conflicti199T → S in CAA67504 (PubMed:9221934).Curated1
Sequence conflicti202 – 203NH → HP in CAA67504 (PubMed:9221934).Curated2
Sequence conflicti224E → D in CAA67504 (PubMed:9221934).Curated1
Sequence conflicti306A → V in BAC29365 (PubMed:16141072).Curated1
Sequence conflicti339A → G in CAA67504 (PubMed:9221934).Curated1
Sequence conflicti343P → A in CAA67504 (PubMed:9221934).Curated1
Sequence conflicti350L → V in CAA67504 (PubMed:9221934).Curated1
Sequence conflicti370N → T in CAA67504 (PubMed:9221934).Curated1
Sequence conflicti373 – 374IV → KG in CAA67504 (PubMed:9221934).Curated2
Sequence conflicti377S → Q in CAA67504 (PubMed:9221934).Curated1
Sequence conflicti382 – 386YQCVA → SQRVG in CAA67504 (PubMed:9221934).Curated5
Sequence conflicti493I → V in CAA67504 (PubMed:9221934).Curated1
Sequence conflicti493I → V in AAI15767 (PubMed:15489334).Curated1
Sequence conflicti686S → G in BAC29365 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_011963496 – 498DPT → GNG in isoform 3. 1 Publication3
Alternative sequenceiVSP_011964499 – 1026Missing in isoform 3. 1 PublicationAdd BLAST528
Alternative sequenceiVSP_011965698 – 705LPEVTPAN → QNTGDQRG in isoform 2. 2 Publications8
Alternative sequenceiVSP_011966706 – 1026Missing in isoform 2. 2 PublicationsAdd BLAST321

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99043 Genomic DNA. Translation: CAA67504.1.
AK036262 mRNA. Translation: BAC29365.1.
AK043507 mRNA. Translation: BAC31563.1.
BC115766 mRNA. Translation: AAI15767.1.
AK173340 mRNA. Translation: BAD32618.1.
CCDSiCCDS20395.1. [Q69Z26-2]
CCDS51866.1. [Q69Z26-1]
RefSeqiNP_001103219.1. NM_001109749.1. [Q69Z26-1]
NP_001103221.1. NM_001109751.1. [Q69Z26-2]
NP_766592.2. NM_173004.3. [Q69Z26-2]
XP_006506256.1. XM_006506193.2. [Q69Z26-1]
XP_006506257.1. XM_006506194.3. [Q69Z26-1]
XP_006506258.1. XM_006506195.3. [Q69Z26-1]
XP_017177088.1. XM_017321599.1. [Q69Z26-2]
UniGeneiMm.321683.

Genome annotation databases

EnsembliENSMUST00000089208; ENSMUSP00000086616; ENSMUSG00000064293. [Q69Z26-1]
ENSMUST00000113258; ENSMUSP00000108883; ENSMUSG00000064293. [Q69Z26-3]
ENSMUST00000113260; ENSMUSP00000108885; ENSMUSG00000064293. [Q69Z26-2]
ENSMUST00000113261; ENSMUSP00000108886; ENSMUSG00000064293. [Q69Z26-2]
ENSMUST00000113264; ENSMUSP00000108889; ENSMUSG00000064293. [Q69Z26-1]
GeneIDi269784.
KEGGimmu:269784.
UCSCiuc009dcr.2. mouse. [Q69Z26-2]
uc009dcu.1. mouse. [Q69Z26-3]
uc009dcv.2. mouse. [Q69Z26-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99043 Genomic DNA. Translation: CAA67504.1.
AK036262 mRNA. Translation: BAC29365.1.
AK043507 mRNA. Translation: BAC31563.1.
BC115766 mRNA. Translation: AAI15767.1.
AK173340 mRNA. Translation: BAD32618.1.
CCDSiCCDS20395.1. [Q69Z26-2]
CCDS51866.1. [Q69Z26-1]
RefSeqiNP_001103219.1. NM_001109749.1. [Q69Z26-1]
NP_001103221.1. NM_001109751.1. [Q69Z26-2]
NP_766592.2. NM_173004.3. [Q69Z26-2]
XP_006506256.1. XM_006506193.2. [Q69Z26-1]
XP_006506257.1. XM_006506194.3. [Q69Z26-1]
XP_006506258.1. XM_006506195.3. [Q69Z26-1]
XP_017177088.1. XM_017321599.1. [Q69Z26-2]
UniGeneiMm.321683.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JXAX-ray2.40A/B25-404[»]
3KLDX-ray2.00A25-404[»]
5E4SX-ray2.50A596-898[»]
ProteinModelPortaliQ69Z26.
SMRiQ69Z26.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4997439.
STRINGi10090.ENSMUSP00000086616.

PTM databases

iPTMnetiQ69Z26.
PhosphoSitePlusiQ69Z26.

Proteomic databases

PaxDbiQ69Z26.
PeptideAtlasiQ69Z26.
PRIDEiQ69Z26.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000089208; ENSMUSP00000086616; ENSMUSG00000064293. [Q69Z26-1]
ENSMUST00000113258; ENSMUSP00000108883; ENSMUSG00000064293. [Q69Z26-3]
ENSMUST00000113260; ENSMUSP00000108885; ENSMUSG00000064293. [Q69Z26-2]
ENSMUST00000113261; ENSMUSP00000108886; ENSMUSG00000064293. [Q69Z26-2]
ENSMUST00000113264; ENSMUSP00000108889; ENSMUSG00000064293. [Q69Z26-1]
GeneIDi269784.
KEGGimmu:269784.
UCSCiuc009dcr.2. mouse. [Q69Z26-2]
uc009dcu.1. mouse. [Q69Z26-3]
uc009dcv.2. mouse. [Q69Z26-1]

Organism-specific databases

CTDi152330.
MGIiMGI:1095737. Cntn4.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG3513. Eukaryota.
ENOG410XSVG. LUCA.
GeneTreeiENSGT00760000118840.
HOGENOMiHOG000059617.
HOVERGENiHBG051047.
InParanoidiQ69Z26.
KOiK06762.
OMAiYWKKEHK.
OrthoDBiEOG091G00X7.
PhylomeDBiQ69Z26.
TreeFamiTF351103.

Miscellaneous databases

ChiTaRSiCntn4. mouse.
PROiQ69Z26.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000064293.
CleanExiMM_CNTN4.
ExpressionAtlasiQ69Z26. baseline and differential.
GenevisibleiQ69Z26. MM.

Family and domain databases

CDDicd00063. FN3. 4 hits.
Gene3Di2.60.40.10. 10 hits.
InterProiIPR033007. Contactin-4.
IPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PANTHERiPTHR10489:SF568. PTHR10489:SF568. 1 hit.
PfamiPF00041. fn3. 2 hits.
PF07679. I-set. 2 hits.
[Graphical view]
SMARTiSM00060. FN3. 4 hits.
SM00409. IG. 6 hits.
SM00408. IGc2. 5 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 6 hits.
SSF49265. SSF49265. 2 hits.
PROSITEiPS50853. FN3. 4 hits.
PS50835. IG_LIKE. 6 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCNTN4_MOUSE
AccessioniPrimary (citable) accession number: Q69Z26
Secondary accession number(s): Q14BL8, Q8BRT6, Q8CBD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: November 23, 2004
Last modified: November 30, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.