Q69Z26 (CNTN4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Contactin-4

Alternative name(s):
Brain-derived immunoglobulin superfamily protein 2
Short name=BIG-2

Gene names

Name:	Cntn4
Synonyms:	Kiaa3024

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1026 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity. May be involved in synaptogenesis. Ref.4
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor. Secreted By similarity.
Tissue specificity	Expressed in the region of developing olfactory neurons. Isoform 2 is expressed in mature sensory cells of the vomeronasal neuroepithelium and at lower level in olfactory neuroepithelium. Ref.4
Developmental stage	Expressed from E14 at a time when developing axons reach the olfactory bulb. Ref.4
Sequence similarities	Belongs to the immunoglobulin superfamily. Contactin family. Contains 4 fibronectin type-III domains. Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell membrane Membrane Secreted
Coding sequence diversity	Alternative splicing
Domain	Immunoglobulin domain Repeat Signal
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	brain development Inferred from sequence or structural similarity. Source: UniProtKB cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of neuron differentiation Inferred from sequence or structural similarity. Source: UniProtKB neuron projection development Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q69Z26-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q69Z26-2)

Also known as: BIG-2A;

The sequence of this isoform differs from the canonical sequence as follows:
     698-705: LPEVTPAN → QNTGDQRG
     706-1026: Missing.

Isoform 3 (identifier: Q69Z26-3)

The sequence of this isoform differs from the canonical sequence as follows:
     496-498: DPT → GNG
     499-1026: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 1000	982	Contactin-4		PRO_0000014713
	Propeptide	1001 – 1026	26	Removed in mature form Potential		PRO_0000014714
Regions
	Domain	32 – 117	86	Ig-like C2-type 1
	Domain	122 – 207	86	Ig-like C2-type 2
	Domain	225 – 311	87	Ig-like C2-type 3
	Domain	316 – 400	85	Ig-like C2-type 4
	Domain	406 – 493	88	Ig-like C2-type 5
	Domain	497 – 586	90	Ig-like C2-type 6
	Domain	596 – 692	97	Fibronectin type-III 1
	Domain	699 – 797	99	Fibronectin type-III 2
	Domain	801 – 896	96	Fibronectin type-III 3
	Domain	901 – 990	90	Fibronectin type-III 4
Amino acid modifications
	Lipidation	1000	1	GPI-anchor amidated serine Potential
	Glycosylation	65	1	N-linked (GlcNAc...) Potential
	Glycosylation	90	1	N-linked (GlcNAc...) Potential
	Glycosylation	191	1	N-linked (GlcNAc...) Potential
	Glycosylation	370	1	N-linked (GlcNAc...) Potential
	Glycosylation	375	1	N-linked (GlcNAc...) Potential
	Glycosylation	466	1	N-linked (GlcNAc...) Potential
	Glycosylation	705	1	N-linked (GlcNAc...) Potential
	Glycosylation	764	1	N-linked (GlcNAc...) Potential
	Glycosylation	858	1	N-linked (GlcNAc...) Potential
	Glycosylation	893	1	N-linked (GlcNAc...) Potential
	Glycosylation	911	1	N-linked (GlcNAc...) Potential
	Glycosylation	929	1	N-linked (GlcNAc...) Potential
	Glycosylation	954	1	N-linked (GlcNAc...) Potential
	Disulfide bond	50 ↔ 100		By similarity
	Disulfide bond	144 ↔ 194		By similarity
	Disulfide bond	247 ↔ 295		By similarity
	Disulfide bond	337 ↔ 384		By similarity
	Disulfide bond	429 ↔ 477		By similarity
	Disulfide bond	519 ↔ 576		By similarity
Natural variations
	Alternative sequence	496 – 498	3	DPT → GNG in isoform 3.		VSP_011963
	Alternative sequence	499 – 1026	528	Missing in isoform 3.		VSP_011964
	Alternative sequence	698 – 705	8	LPEVTPAN → QNTGDQRG in isoform 2.		VSP_011965
	Alternative sequence	706 – 1026	321	Missing in isoform 2.		VSP_011966
Experimental info
	Sequence conflict	64	1	I → L in CAA67504. Ref.1
	Sequence conflict	199	1	T → S in CAA67504. Ref.1
	Sequence conflict	202 – 203	2	NH → HP in CAA67504. Ref.1
	Sequence conflict	224	1	E → D in CAA67504. Ref.1
	Sequence conflict	306	1	A → V in BAC29365. Ref.2
	Sequence conflict	339	1	A → G in CAA67504. Ref.1
	Sequence conflict	343	1	P → A in CAA67504. Ref.1
	Sequence conflict	350	1	L → V in CAA67504. Ref.1
	Sequence conflict	370	1	N → T in CAA67504. Ref.1
	Sequence conflict	373 – 374	2	IV → KG in CAA67504. Ref.1
	Sequence conflict	377	1	S → Q in CAA67504. Ref.1
	Sequence conflict	382 – 386	5	YQCVA → SQRVG in CAA67504. Ref.1
	Sequence conflict	493	1	I → V in CAA67504. Ref.1
	Sequence conflict	686	1	S → G in BAC29365. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 23, 2004. Version 2. Checksum: 34AC35CE432F03AC	FASTA	1,026	113,490
	10 20 30 40 50 60 MRLPWELLVL QSFMLCLADD YTLHGPVFVQ EPSHVMFPLD SEEKKVKLSC EVKGNPKPHI 70 80 90 100 110 120 RWKINGTDVD IGMDFRYSVV DGSLLINNPN KTQDAGTYQC IATNSFGTIV SREAKLQFAY 130 140 150 160 170 180 LENFKTRTRS TVSVRRGQGM VLLCGPPPHS GELSYAWIFN EYPSYQDNRR FVSQETGNLY 190 200 210 220 230 240 IAKVEKSDVG NYTCVVTNTV TNHKVLGPPT PLILRNDGVM GEYEPKIEVQ FPETVPAEKG 250 260 270 280 290 300 TTVKLECFAL GNPVPTILWR RADGKPIARK ARRHKSNGIL EIPNFQQEDA GSYECVAENS 310 320 330 340 350 360 RGKNVAKGQL TFYAQPNWVQ IINDIHVAME ESVFWECKAN GRPKPTYRWL KNGDPLLTRD 370 380 390 400 410 420 RIQIEQGTLN ITIVNLSDAG MYQCVAENKH GVIFSSAELS VIAESPDFSR TLLKRVTLVK 430 440 450 460 470 480 VGGEVVIECK PKASPRPVYT WRKGREILRE NERITISEDG NLRIINVTKS DAGSYTCIAT 490 500 510 520 530 540 NHFGTASSTG NVIVKDPTKV MVPPSSMDVT VGESIVLPCQ VTHDHSLDIV FTWTFNGHLI 550 560 570 580 590 600 DFDKDGDHFE RVGGQDSAGD LMIRNIQLKH AGKYVCMVQT SVDKLSVAAD LIVRGPPGPP 610 620 630 640 650 660 EAVTIDEITD TTAQLSWRPG PDNHSPITMY VIQARTPFSV GWQAVNTVPD LVDGKTFTAT 670 680 690 700 710 720 VVGLNPWVEY EFRTVAANVI GIGEPSRPSE KRRTEEALPE VTPANVSGGG GSKSELVITW 730 740 750 760 770 780 ETVPEELQNG RGFGYVVAFR PHGKMIWMLT VLASADASRY VFRNESVRPF SPFEVKVGVF 790 800 810 820 830 840 NNKGEGPFSP TTLVYSAEEE PTKPPASIFA RSLSATDIEV FWASPIGKNR GRIQGYEVKY 850 860 870 880 890 900 WRHDDKEENA KKIRTVGNQT STKITNLKGS ALYHLSVKAY NSAGTGPSSA TVNVTTRKPP 910 920 930 940 950 960 PSQPPGNIIW NSSDSKIILN WDQVKALDNE SEVKGYKVLY RWNRQSSTSV IETNKTSVEL 970 980 990 1000 1010 1020 SLPFDEDYII EIKPFSDGGD GSSSEQIRIP KISNSYARGS GASTSNACTL SAISTIMISL TARSSL
	Isoform 2 (BIG-2A) [UniParc]. Checksum: 1AA5A089B21237D7	FASTA	705	78,425
	10 20 30 40 50 60 MRLPWELLVL QSFMLCLADD YTLHGPVFVQ EPSHVMFPLD SEEKKVKLSC EVKGNPKPHI 70 80 90 100 110 120 RWKINGTDVD IGMDFRYSVV DGSLLINNPN KTQDAGTYQC IATNSFGTIV SREAKLQFAY 130 140 150 160 170 180 LENFKTRTRS TVSVRRGQGM VLLCGPPPHS GELSYAWIFN EYPSYQDNRR FVSQETGNLY 190 200 210 220 230 240 IAKVEKSDVG NYTCVVTNTV TNHKVLGPPT PLILRNDGVM GEYEPKIEVQ FPETVPAEKG 250 260 270 280 290 300 TTVKLECFAL GNPVPTILWR RADGKPIARK ARRHKSNGIL EIPNFQQEDA GSYECVAENS 310 320 330 340 350 360 RGKNVAKGQL TFYAQPNWVQ IINDIHVAME ESVFWECKAN GRPKPTYRWL KNGDPLLTRD 370 380 390 400 410 420 RIQIEQGTLN ITIVNLSDAG MYQCVAENKH GVIFSSAELS VIAESPDFSR TLLKRVTLVK 430 440 450 460 470 480 VGGEVVIECK PKASPRPVYT WRKGREILRE NERITISEDG NLRIINVTKS DAGSYTCIAT 490 500 510 520 530 540 NHFGTASSTG NVIVKDPTKV MVPPSSMDVT VGESIVLPCQ VTHDHSLDIV FTWTFNGHLI 550 560 570 580 590 600 DFDKDGDHFE RVGGQDSAGD LMIRNIQLKH AGKYVCMVQT SVDKLSVAAD LIVRGPPGPP 610 620 630 640 650 660 EAVTIDEITD TTAQLSWRPG PDNHSPITMY VIQARTPFSV GWQAVNTVPD LVDGKTFTAT 670 680 690 700 VVGLNPWVEY EFRTVAANVI GIGEPSRPSE KRRTEEAQNT GDQRG
	Isoform 3 [UniParc]. Checksum: 32B0AFB2EF8804F0	FASTA	498	55,719
	10 20 30 40 50 60 MRLPWELLVL QSFMLCLADD YTLHGPVFVQ EPSHVMFPLD SEEKKVKLSC EVKGNPKPHI 70 80 90 100 110 120 RWKINGTDVD IGMDFRYSVV DGSLLINNPN KTQDAGTYQC IATNSFGTIV SREAKLQFAY 130 140 150 160 170 180 LENFKTRTRS TVSVRRGQGM VLLCGPPPHS GELSYAWIFN EYPSYQDNRR FVSQETGNLY 190 200 210 220 230 240 IAKVEKSDVG NYTCVVTNTV TNHKVLGPPT PLILRNDGVM GEYEPKIEVQ FPETVPAEKG 250 260 270 280 290 300 TTVKLECFAL GNPVPTILWR RADGKPIARK ARRHKSNGIL EIPNFQQEDA GSYECVAENS 310 320 330 340 350 360 RGKNVAKGQL TFYAQPNWVQ IINDIHVAME ESVFWECKAN GRPKPTYRWL KNGDPLLTRD 370 380 390 400 410 420 RIQIEQGTLN ITIVNLSDAG MYQCVAENKH GVIFSSAELS VIAESPDFSR TLLKRVTLVK 430 440 450 460 470 480 VGGEVVIECK PKASPRPVYT WRKGREILRE NERITISEDG NLRIINVTKS DAGSYTCIAT 490 NHFGTASSTG NVIVKGNG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel splice variant of the cell adhesion molecule BIG-2 is expressed in the olfactory and vomeronasal neuroepithelia." Mimmack M.L., Saito H., Evans G., Bresler M., Keverne E.B., Emson P.C. Brain Res. Mol. Brain Res. 47:345-350(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2). Tissue: Nasal epithelium.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Strain: C57BL/6J. Tissue: Brain cortex and Cerebellum.
[3]	"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H. DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 129-1026 (ISOFORM 1). Tissue: Fetal brain.
[4]	"Expression of olfactory receptors, G-proteins and AxCAMs during the development and maturation of olfactory sensory neurons in the mouse." Saito H., Mimmack M., Kishimoto J., Keverne E.B., Emson P.C. Brain Res. Dev. Brain Res. 110:69-81(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X99043 Genomic DNA. Translation: CAA67504.1. AK036262 mRNA. Translation: BAC29365.1. AK043507 mRNA. Translation: BAC31563.1. AK173340 mRNA. Translation: BAD32618.1.
IPI	IPI00228295. IPI00480306. IPI00480571.
RefSeq	NP_001103219.1. NM_001109749.1. NP_001103221.1. NM_001109751.1. NP_766592.2. NM_173004.3.
UniGene	Mm.321683.
3D structure databases
ProteinModelPortal	Q69Z26.
SMR	Q69Z26. Positions 25-995.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000108889.
Proteomic databases
PaxDb	Q69Z26.
PRIDE	Q69Z26.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000089208; ENSMUSP00000086616; ENSMUSG00000064293. ENSMUST00000113258; ENSMUSP00000108883; ENSMUSG00000064293. ENSMUST00000113260; ENSMUSP00000108885; ENSMUSG00000064293. ENSMUST00000113261; ENSMUSP00000108886; ENSMUSG00000064293. ENSMUST00000113264; ENSMUSP00000108889; ENSMUSG00000064293.
GeneID	269784.
KEGG	mmu:269784.
UCSC	uc009dcr.2. mouse. uc009dcu.1. mouse. uc009dcv.2. mouse.
Organism-specific databases
CTD	152330.
MGI	MGI:1095737. Cntn4.
Rouge	Search...
Phylogenomic databases
eggNOG	NOG293951.
GeneTree	ENSGT00550000074380.
HOGENOM	HOG000059617.
HOVERGEN	HBG051047.
InParanoid	Q69Z26.
KO	K06762.
OMA	IEQGTLN.
OrthoDB	EOG48GW2G.
Gene expression databases
ArrayExpress	Q69Z26.
Bgee	Q69Z26.
CleanEx	MM_CNTN4.
Genevestigator	Q69Z26.
GermOnline	ENSMUSG00000064293. Mus musculus.
Family and domain databases
Gene3D	2.60.40.10. 10 hits.
InterPro	IPR003961. Fibronectin_type3. IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR013098. Ig_I-set. IPR003599. Ig_sub. IPR003598. Ig_sub2. [Graphical view]
Pfam	PF00041. fn3. 2 hits. PF07679. I-set. 5 hits. [Graphical view]
SMART	SM00060. FN3. 4 hits. SM00409. IG. 2 hits. SM00408. IGc2. 4 hits. [Graphical view]
SUPFAM	SSF49265. FN_III-like. 4 hits.
PROSITE	PS50853. FN3. 4 hits. PS50835. IG_LIKE. 6 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CNTN4. mouse.
NextBio	393030.
SOURCE	Search...

Entry information

Entry name

CNTN4_MOUSE

Accession

Primary (citable) accession number: Q69Z26
Secondary accession number(s): Q8BRT6, Q8CBD3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 23, 2004
Last sequence update:	November 23, 2004
Last modified:	May 1, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program