Reviewed, UniProtKB/Swiss-Prot Q69XV9 (EXP16_ORYSJ)

Names and origin

Protein names

Recommended name:
    Expansin-A16
Alternative name(s):
    OsEXPA16
    Alpha-expansin-16
    OsEXP16
    OsaEXPa1.27

Gene names

Name:	EXPA16
Synonyms:	EXP16
Ordered Locus Names:	Os06g0621900, LOC_Os06g41700
ORF Names:	P0040H10.29

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May cause loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found. May be required for rapid internodal elongation in deepwater rice during submergence By similarity.
Subcellular location	Secreted › cell wall By similarity. Membrane; Peripheral membrane protein By similarity.
Tissue specificity	Expressed in panicles and flowers. Ref.4
Developmental stage	Expressed in the growing regions of roots, internodes and leaves. Ref.2
Induction	By gibberellin (GA3) and wounding.
Sequence similarities	Belongs to the expansin family. Expansin A subfamily. Contains 1 expansin-like CBD domain. Contains 1 expansin-like EG45 domain.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Membrane Secreted
Domain	Signal
Gene Ontology (GO)
Biological process	plant-type cell wall organization Inferred from electronic annotation. Source: InterPro
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 260	241	Expansin-A16		PRO_0000251995
Regions
	Domain	52 – 166	115	Expansin-like EG45
	Domain	176 – 255	80	Expansin-like CBD
Experimental info
	Sequence conflict	160	1	S → N in AAL24488. Ref.2
	Sequence conflict	164	1	A → P in AAL24488. Ref.2
	Sequence conflict	168	1	R → S in AAL24488. Ref.2
	Sequence conflict	172	1	T → P in AAL24488. Ref.2
	Sequence conflict	175	1	A → P in AAL24488. Ref.2
	Sequence conflict	179 – 190	12	QVLIT…AADGE → KVVIKKRGGERK in AAL24488. Ref.2
	Sequence conflict	194	1	V → G in AAL24488. Ref.2
	Sequence conflict	198 – 218	21	GSRTG…WQCDA → SLKPRVTPKEKEGGPELTVR R in AAL24488. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q69XV9-1 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: B793143CB9E43000	FASTA	260	27,998
	10 20 30 40 50 60 MSSVLLFLLL LLLSGVSLSG CIRLGNGGYE EWRMGSATYI KESLGHPLND GGGACGYGDL 70 80 90 100 110 120 DIFRYGRYTA GVSGALFGRG SACGGCYEVR CVNHVLWCLR GSPTVVVTAT DFCAPNLGLS 130 140 150 160 170 180 DDYGGWCNFP KEHFEMSEAA FLRVAKAKAD IVPVQFRRVS CDRAGGMRFT ITGGASFLQV 190 200 210 220 230 240 LITNVAADGE VAAVKVKGSR TGWIPMGRNW GQNWQCDADL RGQPLSFEVT GGRGRTVVAY 250 260 SVAPPDWMFA QTFEGKQFVE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Oryza sativa nipponbare chromosome 6 genomic DNA sequence." The international rice genome sequencing project (IRGSP) consortium Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Expression of alpha-expansin and expansin-like genes in deepwater rice." Lee Y., Kende H. Plant Physiol. 130:1396-1405(2002) [PubMed: 12428004] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 52-260, DEVELOPMENTAL STAGE.
[3]	"Nomenclature for members of the expansin superfamily of genes and proteins." Kende H., Bradford K.J., Brummell D.A., Cho H.-T., Cosgrove D.J., Fleming A.J., Gehring C., Lee Y., McQueen-Mason S.J., Rose J.K.C., Voesenek L.A.C. Plant Mol. Biol. 55:311-314(2004) [PubMed: 15604683] [Abstract] Cited for: NOMENCLATURE.
[4]	"Characterization and transcriptional expression of the alpha-expansin gene family in rice." Shin J.-H., Jeong D.-H., Park M.C., An G. Mol. Cells 20:210-218(2005) [PubMed: 16267395] [Abstract] Cited for: TISSUE SPECIFICITY.

Web resources

EXPANSIN homepage

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP003539 Genomic DNA. Translation: BAD35368.1. AF394552 Genomic DNA. Translation: AAL24488.1.
RefSeq	NP_001058095.1.
UniGene	Os.54103
3D structure databases
HSSP	HSSP built from PDB template 1N10 based on UniProtKB P43213.
SMR	Q69XV9. Positions 30-259.
ModBase	Search...
Genome annotation databases
GeneID	4341549.
KEGG	osa:4341549.
Organism-specific databases
Gramene	Q69XV9.
Family and domain databases
InterPro	IPR009009. Barwin-related_endoglucanase. IPR007112. Expan_endogl. IPR007118. Expan_Lol_pI. IPR007117. Expan_Lol_pI_C. IPR002963. Expansin. IPR014734. Pollen_allergen. IPR005132. RlpA. [Graphical view]
Gene3D	G3DSA:2.60.40.760. Expan_Lol_pI_C. 1 hit. G3DSA:2.40.40.40. Pollen_allergen. 1 hit.
Pfam	PF03330. DPBB_1. 1 hit. PF01357. Pollen_allerg_1. 1 hit. [Graphical view]
PRINTS	PR01226. EXPANSIN. PR01225. EXPANSNFAMLY.
PROSITE	PS50843. EXPANSIN_CBD. 1 hit. PS50842. EXPANSIN_EG45. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EXP16_ORYSJ

Accession

Primary (citable) accession number: Q69XV9
Secondary accession number(s): Q946I5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	September 13, 2004
Last modified:	January 19, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)