Q69XJ4 (HMOX1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heme oxygenase 1, chloroplastic
EC=1.14.99.3

Alternative name(s):
Protein PHOTOPERIODIC SENSITIVITY 5

Gene names

Name:	HO1
Synonyms:	HY1, SE5
Ordered Locus Names:	Os06g0603000, LOC_Os06g40080
ORF Names:	OsJ_21897, P0486H12.31

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the opening of the heme ring to form the open-chain tetrapyrrole biliverdin IX with the release of iron and carbon monoxide (CO). Is a key enzyme in the synthesis of the chromophore of the phytochrome family of plant photoreceptors. Essential for photoperiod response and repression of flowering through cytochromes that inhibit flowering by affecting both HD1 and EHD1 flowering pathways. Ref.6 Ref.7
Catalytic activity	Heme + 3 AH2 + 3 O2 = biliverdin + Fe2+ + CO + 3 A + 3 H2O.
Subcellular location	Plastid › chloroplast.
Disruption phenotype	Pale green and very early flowering phenotype and complete deficiency in photoperiodic response. Lack of photoreversible phytochromes and responses of coleoptile elongation by light pulses. Ref.6 Ref.7
Sequence similarities	Belongs to the heme oxygenase family.

Ontologies

Keywords
Biological process	Photosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chloroplast-nucleus signaling pathway Inferred from electronic annotation. Source: EnsemblPlants/Gramene heme oxidation Inferred from sequence or structural similarity Ref.6. Source: Gramene inflorescence development Inferred from genetic interaction Ref.6. Source: Gramene photoperiodism, flowering Inferred from mutant phenotype Ref.7. Source: UniProtKB photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW phytochromobilin biosynthetic process Inferred from electronic annotation. Source: EnsemblPlants/Gramene red, far-red light phototransduction Inferred from electronic annotation. Source: EnsemblPlants/Gramene regulation of meristem growth Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	heme binding Inferred from electronic annotation. Source: EnsemblPlants/Gramene heme oxygenase (decyclizing) activity Inferred from sequence or structural similarity Ref.6. Source: Gramene metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 64	64	Chloroplast Potential
	Chain	65 – 289	225	Heme oxygenase 1, chloroplastic		PRO_0000412189
Sites
	Metal binding	96	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q69XJ4 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: C03B0103AD2E6E91	FASTA	289	31,918
	10 20 30 40 50 60 MAPAAASLTA PNALAATSLP FLHGRKSGGG GVSVHAGAPS PSRAVAVVAR RLWGSASSSR 70 80 90 100 110 120 RMVVAAATAA EMAPAASGEE GKPFVEEMRA VAMRLHTKDQ AKEGEKEPQA PPVARWEPSV 130 140 150 160 170 180 DGYLRFLVDS KLVFETLETI VDRAAVPWYA EFRNTGLERS EQLKKDLEWF KEQGHTIPEP 190 200 210 220 230 240 SAPGTTYASY LEELAEKDSQ AFICHFYNVY FAHTAGGRMI GKKVSENILN KKELEFYKWE 250 260 270 280 GNLSQLLQNV RNKLNEVASS WTREEKDHCL DETEKSFSYS GDLLRHIFT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of heme oxygenase-1 from Oryza sativa." Xu S., Wu T.T., Lu H., Ling T.F., Xuan W., Sun Y.G., Cao Z., Shen W.B. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[5]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[6]	"Phytochromes confer the photoperiodic control of flowering in rice (a short-day plant)." Izawa T., Oikawa T., Tokutomi S., Okuno K., Shimamoto K. Plant J. 22:391-399(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[7]	"Analysis of PHOTOPERIOD SENSITIVITY5 sheds light on the role of phytochromes in photoperiodic flowering in rice." Andres F., Galbraith D.W., Talon M., Domingo C. Plant Physiol. 151:681-690(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EU781632 mRNA. Translation: ACE98543.1. AP003615 Genomic DNA. Translation: BAD35463.1. AP008212 Genomic DNA. Translation: BAF19925.1. CM000143 Genomic DNA. Translation: EEE65978.1. AK068856 mRNA. Translation: BAG91127.1. AK104955 mRNA. Translation: BAG97040.1.
RefSeq	NP_001058011.1. NM_001064546.1.
UniGene	Os.20352.
3D structure databases
ProteinModelPortal	Q69XJ4.
ModBase	Search...
Proteomic databases
PRIDE	Q69XJ4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os06g40080.1; LOC_Os06g40080.1; LOC_Os06g40080.
GeneID	4341462.
KEGG	osa:4341462.
Organism-specific databases
Gramene	Q69XJ4.
Phylogenomic databases
eggNOG	NOG308332.
HOGENOM	HOG000265822.
KO	K00510.
OMA	GGRMIGR.
ProtClustDB	CLSN2919695.
Gene expression databases
ArrayExpress	Q69XJ4.
Family and domain databases
Gene3D	1.20.910.10. 1 hit.
InterPro	IPR016053. Haem_Oase-like. IPR016084. Haem_Oase-like_multi-hlx. IPR016951. Haem_Oase_decyc_pln. [Graphical view]
Pfam	PF01126. Heme_oxygenase. 1 hit. [Graphical view]
PIRSF	PIRSF030219. Heme_Oase_decyc_pln. 1 hit.
SUPFAM	SSF48613. Heme_oxygenase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HMOX1_ORYSJ

Accession

Primary (citable) accession number: Q69XJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 27, 2011
Last sequence update:	September 13, 2004
Last modified:	April 3, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program