Reviewed, UniProtKB/Swiss-Prot Q69TB1 (HEMH1_ORYSJ)

Names and origin

Protein names

Recommended name:
    Ferrochelatase-1, chloroplastic
    EC=4.99.1.1
Alternative name(s):
    Ferrochelatase I
    Protoheme ferro-lyase 1
    Heme synthetase 1

Gene names

Ordered Locus Names:	Os09g0297000, LOC_Os09g12560
ORF Names:	P0592C05.24

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX.
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+.
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1.
Subcellular location	Plastid › chloroplast By similarity.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Iron
Molecular function	Lyase
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 482		Ferrochelatase-1, chloroplastic		PRO_0000376075

Sequences

	Sequence		Length	Mass (Da)
	Q69TB1-1 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: 63F53C2B81373661	FASTA	482	53,252
	10 20 30 40 50 60 MECVRSGSGV LDPRCSPRFL GKKGGSLTSC GKATSTNLAI CTKHEQNLHG NVKPSQLAAS 70 80 90 100 110 120 GSSYSVHRSP VLKQRQNLSA RSTSADVYTT FDENVRAVSS HAAEEKVGVL LLNLGGPETL 130 140 150 160 170 180 DDVQPFLFNL FADPDIIRLP RLFRFLQRPL AKLISTFRAP KSKEGYASIG GGSPLRKITD 190 200 210 220 230 240 EQANALKVAL KKKNLNANIY VGMRYWYPFT EEAIDQIKKD KITKLVVLPL YPQYSISTSG 250 260 270 280 290 300 SSIRVLQNIV KEDSYFAGLP ISIIESWYQR DGYVKSMADL IEKELSIFSN PEEVMIFFSA 310 320 330 340 350 360 HGVPLTYVTD AGDPYRDQME DCIALIMGEL KSRGILNSHT LAYQSRVGPV QWLKPYTDEV 370 380 390 400 410 420 LVELGQQGVK SLLAVPVSFV SEHIETLEEI DMEYKELALE SGIENWGRVP ALGCTSSFIS 430 440 450 460 470 480 DLADAVVEAL PSASALVTKK VDESDSDMDL MHYLSKMFFG SILAFVLLLS PRLISAFRNT LL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP004756 Genomic DNA. Translation: BAD33213.1. AP008215 Genomic DNA. Translation: BAF24717.1. AK068174 mRNA. Translation: BAG90790.1.
RefSeq	NP_001062803.1.
UniGene	Os.9742
3D structure databases
HSSP	HSSP built from PDB template 1HRK based on UniProtKB P22830.
ModBase	Search...
Genome annotation databases
GeneID	4346651.
KEGG	osa:4346651.
Organism-specific databases
Gramene	Q69TB1.
Phylogenomic databases
HOGENOM	HBG697135.
PhylomeDB	Q69TB1.
Family and domain databases
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00109. hemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH1_ORYSJ

Accession

Primary (citable) accession number: Q69TB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	September 13, 2004
Last modified:	February 9, 2010
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)