Reviewed, UniProtKB/Swiss-Prot Q69SV0 (APX8_ORYSJ)

Names and origin

Protein names

Recommended name:
    Probable L-ascorbate peroxidase 8, chloroplastic
    EC=1.11.1.11
Alternative name(s):
    OsAPx08

Gene names

Name:	APX8
Ordered Locus Names:	Os02g0553200, LOC_Os02g34810
ORF Names:	P0470G10.5

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a key role in hydrogen peroxide removal By similarity.
Catalytic activity	L-ascorbate + H(2)O(2) = dehydroascorbate + 2 H(2)O.
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group By similarity. Binds 1 potassium or calcium ion per subunit By similarity.
Subcellular location	Plastid › chloroplast membrane; Single-pass membrane proteinPotential.
Sequence similarities	Belongs to the peroxidase family. Ascorbate peroxidase subfamily.
Sequence caution	The sequence BAD33296.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Cellular component	Chloroplast Membrane Plastid
Domain	Transit peptide Transmembrane
Ligand	Calcium Heme Iron Metal-binding Potassium
Molecular function	Oxidoreductase Peroxidase
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-ascorbate peroxidase activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 76	76	Chloroplast Potential
	Chain	77 – 478	402	Probable L-ascorbate peroxidase 8, chloroplastic		PRO_0000042658
Regions
	Transmembrane	458 – 478	21	Potential
	Compositional bias	15 – 26	12	Poly-Pro
	Compositional bias	350 – 394	45	Pro-rich
Sites
	Active site	117	1	Proton acceptor By similarity
	Metal binding	246	1	Iron (heme axial ligand) By similarity
	Metal binding	247	1	Potassium or calcium By similarity
	Metal binding	279	1	Potassium or calcium By similarity
	Metal binding	286	1	Potassium or calcium By similarity
	Site	113	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q69SV0-1 [UniParc]. Last modified October 25, 2005. Version 2. Checksum: EE5DFC9821EA87C2	FASTA	478	51,188
	10 20 30 40 50 60 MAERIAASLL PAASPSPAPS PPPPRPRVSA AAAASFPCCS TSAGGLRLRS RPSRFPQKAA 70 80 90 100 110 120 TTRSGRAGAG ARAVVRCMAA AAVAASDAAQ LKSAREDIRE ILKTTYCHPI MVRLGWHDSG 130 140 150 160 170 180 TYDKNIEEWP QRGGADGSLR FDAELSHGAN AGLINALKLI QPIKDKYPGI TYADLFQLAS 190 200 210 220 230 240 ATAIEEAGGP KIPMKYGRVD VTAAEQCPPE GRLPDAGPRV PADHLREVFY RMGLDDKEIV 250 260 270 280 290 300 ALSGAHTLGR SRPDRSGWGK PETKYTKDGP GEPGGQSWTV EWLKFDNSYF KDIKEQRDQD 310 320 330 340 350 360 LLVLPTDAAL FEDPSFKVYA EKYAEDQEAF FKDYAEAHAK LSDLGAKFDP PEGFSLDDEP 370 380 390 400 410 420 AVEEKDPEPA PAPAAAPPPP PVEEKKEAEP TPVPVTVGAA VASSPADDNN GAAPQPEPFV 430 440 450 460 470 AAKYSYGKKE LSDSMKQKIR AEYEGFGGSP DKPLQSNYFL NIMLLIGGLA FLTSLLGS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The expression of rice chloroplastic ascorbate peroxidase genes during greening." Morita S., Kotani T., Kaminaka H., Masumura T., Tanaka K. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare.
[2]	"Oryza sativa nipponbare chromosome 2 genomic DNA sequence." The international rice genome sequencing project (IRGSP) consortium Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Analysis of the molecular evolutionary history of the ascorbate peroxidase gene family: inferences from the rice genome." Teixeira F.K., Menezes-Benavente L., Margis R., Margis-Pinheiro M. J. Mol. Evol. 59:761-770(2004) [PubMed: 15599508] [Abstract] Cited for: NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB114856 mRNA. Translation: BAC79363.1. AP004876 Genomic DNA. Translation: BAD33296.1. Sequence problems.
RefSeq	NP_001047111.1.
UniGene	Os.26815
3D structure databases
HSSP	HSSP built from PDB template 1IYN based on UniProtKB Q8LNY5.
SMR	Q69SV0. Positions 84-357.
ModBase	Search...
Protein family/group databases
PeroxiBase	1872. OsAPx08.
Genome annotation databases
GeneID	4329643.
KEGG	osa:4329643.
Organism-specific databases
Gramene	Q69SV0.
Family and domain databases
InterPro	IPR002207. Asc_perxdse. IPR002016. Haem_peroxidase_pln/fun/bac. [Graphical view]
Pfam	PF00141. peroxidase. 1 hit. [Graphical view]
PRINTS	PR00459. ASPEROXIDASE. PR00458. PEROXIDASE.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. False negative. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

APX8_ORYSJ

Accession

Primary (citable) accession number: Q69SV0
Secondary accession number(s): Q7XJ01

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	October 25, 2005
Last modified:	November 25, 2008
This is version 34 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)