Q69ST6 (PID13_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein disulfide isomerase-like 1-3
Short name=OsPDIL1-3
EC=5.3.4.1

Gene names

Name:	PDIL1-3
Ordered Locus Names:	Os02g0554900, LOC_Os02g34940
ORF Names:	P0470G10.25

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	545 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts as a protein-folding catalyst that interacts with nascent polypeptides to catalyze the formation, isomerization, and reduction or oxidation of disulfide bonds. May play a role in storage protein biogenesis By similarity.
Catalytic activity	Catalyzes the rearrangement of -S-S- bonds in proteins.
Subcellular location	Endoplasmic reticulum lumen Probable.
Sequence similarities	Belongs to the protein disulfide isomerase family. Contains 2 thioredoxin domains.
Sequence caution	The sequence BAG93318.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Redox-active center Repeat Signal
Molecular function	Isomerase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro embryo development Inferred from electronic annotation. Source: EnsemblPlants/Gramene glycerol ether metabolic process Inferred from electronic annotation. Source: InterPro protein folding Inferred from electronic annotation. Source: GOC regulation of programmed cell death Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to cadmium ion Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to endoplasmic reticulum stress Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to salt stress Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to zinc ion Inferred from electronic annotation. Source: EnsemblPlants/Gramene seed development Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	chloroplast Inferred from electronic annotation. Source: EnsemblPlants/Gramene endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell lytic vacuole within protein storage vacuole Inferred from electronic annotation. Source: EnsemblPlants/Gramene plant-type cell wall Inferred from electronic annotation. Source: EnsemblPlants/Gramene plasma membrane Inferred from electronic annotation. Source: EnsemblPlants/Gramene thylakoid Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro protein disulfide isomerase activity Inferred from electronic annotation. Source: EC protein disulfide oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – ?
	Chain	? – 545		Protein disulfide isomerase-like 1-3		PRO_0000400030
Regions
	Domain	55 – 189	135	Thioredoxin 1
	Domain	403 – 545	143	Thioredoxin 2
	Motif	542 – 545	4	Prevents secretion from ER By similarity
Sites
	Active site	107	1	Nucleophile By similarity
	Active site	110	1	Nucleophile By similarity
	Active site	453	1	Nucleophile By similarity
	Active site	456	1	Nucleophile By similarity
	Site	108	1	Contributes to redox potential value By similarity
	Site	109	1	Contributes to redox potential value By similarity
	Site	175	1	Lowers pKa of C-terminal Cys of first active site By similarity
	Site	454	1	Contributes to redox potential value By similarity
	Site	455	1	Contributes to redox potential value By similarity
	Site	515	1	Lowers pKa of C-terminal Cys of second active site By similarity
Amino acid modifications
	Glycosylation	87	1	N-linked (GlcNAc...) Potential
	Glycosylation	349	1	N-linked (GlcNAc...) Potential
	Disulfide bond	107 ↔ 110		Redox-active By similarity
	Disulfide bond	453 ↔ 456		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q69ST6 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 362611FD2AD09ED1	FASTA	545	59,075
	10 20 30 40 50 60 MWPRAPATPP PPPWPSKPSA ASRSALRRLD LDDGRRQGTE GEENHAPLLC SPAMASSTAF 70 80 90 100 110 120 AAAFALLLLA SSAAAEGEAV LTLDAGNFTE VVGAHDFIVV EFYAPWCGHC NQLAPEYEAA 130 140 150 160 170 180 AAALRSHDPP VVLAKVDASA DLNRGLAGEH GVQGYPTIRI LRDRGARSHN YAGPRDAAGI 190 200 210 220 230 240 VAYLKRQAGP ASVEIAASAS PPAADSIAND GVVVVGVFPE LSGSEFESFM AVAEKMRADY 250 260 270 280 290 300 DFRHTTDAGV LPRGDRTVRG PLVRLFKPFD ELFVDSQDFD RDALEKFIES SGFPTVVTFD 310 320 330 340 350 360 TSPANQKYLL KYFDNAGTKA MLFLSFSDDR AEEFRTQFHE AANQYSANNI SFLIGDVTAS 370 380 390 400 410 420 QGAFQYFGLK ESEVPLVFIL ASKSKYIKPT VEPDQILPYL KEFTEGTLAP HVKSEPIPEV 430 440 450 460 470 480 NDQPVKTVVA DNLREVVFNS GKNVLLEFYA PWCGHCQKLA PILEEVAVSL KDDEDVVIAK 490 500 510 520 530 540 MDGTANDVPS DFAVEGYPSM YFYSSGGNLL PYDGRTAEEI IDFITKNKGS RPGEATTTES VKDEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 27-545. Strain: cv. Nipponbare.
[4]	"Phylogenetic analyses identify 10 classes of the protein disulfide isomerase family in plants, including single-domain protein disulfide isomerase-related proteins." Houston N.L., Fan C., Xiang J.Q., Schulze J.M., Jung R., Boston R.S. Plant Physiol. 137:762-778(2005) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[5]	"The protein disulfide isomerase gene family in bread wheat (T. aestivum L.)." d'Aloisio E., Paolacci A.R., Dhanapal A.P., Tanzarella O.A., Porceddu E., Ciaffi M. BMC Plant Biol. 10:101-101(2010) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP004876 Genomic DNA. Translation: BAD33310.1. AP008208 Genomic DNA. Translation: BAF09033.1. AK073161 mRNA. Translation: BAG93318.1. Different initiation.
RefSeq	NP_001047119.1. NM_001053654.1.
UniGene	Os.53357.
3D structure databases
ProteinModelPortal	Q69ST6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os02g34940.1; LOC_Os02g34940.1; LOC_Os02g34940.
GeneID	4329652.
KEGG	dosa:Os02t0554900-01. osa:4329652.
Organism-specific databases
Gramene	Q69ST6.
Phylogenomic databases
eggNOG	COG0526.
HOGENOM	HOG000162459.
KO	K09580.
OMA	YSANNIS.
ProtClustDB	CLSN2679750.
Gene expression databases
ArrayExpress	Q69ST6.
Family and domain databases
Gene3D	3.40.30.10. 3 hits.
InterPro	IPR005792. Prot_disulphide_isomerase. IPR005746. Thioredoxin. IPR012336. Thioredoxin-like_fold. IPR017937. Thioredoxin_CS. IPR013766. Thioredoxin_domain. [Graphical view]
Pfam	PF00085. Thioredoxin. 2 hits. [Graphical view]
PRINTS	PR00421. THIOREDOXIN.
SUPFAM	SSF52833. Thiordxn-like_fd. 4 hits.
TIGRFAMs	TIGR01130. ER_PDI_fam. 1 hit.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00194. THIOREDOXIN_1. 2 hits. PS51352. THIOREDOXIN_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PID13_ORYSJ

Accession

Primary (citable) accession number: Q69ST6
Secondary accession number(s): B7ELS1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 2, 2010
Last sequence update:	October 25, 2004
Last modified:	April 3, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program