Cytochrome b6-f complex iron-sulfur subunit, chloroplastic precursor

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Reviewed, UniProtKB/Swiss-Prot Q69S39 (UCRIA_ORYSJ)

Last modified October 13, 2009. Version 44. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Cytochrome b6-f complex iron-sulfur subunit, chloroplastic
    EC=1.10.99.1
Alternative name(s):
    Rieske iron-sulfur protein
      Short name=RISP
      Short name=ISP
    Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein

Gene names

Name:	petC
Ordered Locus Names:	Os07g0556200, LOC_Os07g37030
ORF Names:	OSJNBa0058I18.25

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

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Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions By similarity.
Catalytic activity	Plastoquinol-1 + 2 oxidized plastocyanin = plastoquinone + 2 reduced plastocyanin.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity.
Subunit structure	The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are petG, petL, petM and petN. The complex functions as a dimer By similarity.
Subcellular location	Plastid › chloroplast thylakoid membrane; Single-pass membrane protein By similarity. Note: The transmembrane helix obliquely spans the membrane in one monomer, and its extrinsic C-terminal domain is part of the other monomer By similarity.
Miscellaneous	This protein is 1 of 2 subunits of the cytochrome b6-f complex that are encoded in the nucleus. The Rieske iron-sulfur protein is a high potential 2Fe-2S protein.
Sequence similarities	Contains 1 Rieske domain.

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Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Chloroplast Membrane Plastid Thylakoid
Domain	Transit peptide Transmembrane
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell thylakoid membrane Inferred from electronic annotation. Source: InterPro
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW plastoquinol-plastocyanin reductase activity Inferred from electronic annotation. Source: EC ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 46

Chloroplast Ref.3

Chain

47 – 225

179

Cytochrome b6-f complex iron-sulfur subunit, chloroplastic

PRO_0000030690

Regions

Transmembrane

69 – 89

Potential

Domain

112 – 208

Rieske

Sites

Metal binding

154

Iron-sulfur (2Fe-2S) By similarity

Metal binding

156

Iron-sulfur (2Fe-2S); via pros nitrogen By similarity

Metal binding

172

Iron-sulfur (2Fe-2S) By similarity

Metal binding

175

Iron-sulfur (2Fe-2S); via pros nitrogen By similarity

Amino acid modifications

Disulfide bond

159 ↔ 174

By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

Q69S39-1 [UniParc].

Last modified September 13, 2004. Version 1.
Checksum: 3CB5116F297AF8B0
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FASTA

225

23,884

        10         20         30         40         50         60 
MASTALSTAS NPTQLCRSRA SLGKPVKGLG FGRERVPRTA TTITCQAASS IPADRVPDMG 

        70         80         90        100        110        120 
KRQLMNLLLL GAISLPTVGM LVPYGAFFIP AGSGNAGGGQ VAKDKLGNDV LAEEWLKTHG 

       130        140        150        160        170        180 
PNDRTLTQGL KGDPTYLVVE ADKTLATYGI NAVCTHLGCV VPWNAAENKF ICPCHGSQYN 

       190        200        210        220 
NQGRVVRGPA PLSLALVHAD VDDGKVLFVP WVETDFRTGD NPWWA

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of cytochrome B6/F-complex iron-sulfur protein in rice." Lee J.-S. Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Ilpoom. Tissue: Seedling leaf.
[2]	"Oryza sativa nipponbare chromosome 7 genomic DNA sequence." The international rice genome sequencing project (IRGSP) consortium Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003." Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K. Nucleic Acids Res. 32:D388-D392(2004) [PubMed: 14681440] [Abstract] Cited for: PROTEIN SEQUENCE OF 47-56. Strain: cv. Nipponbare. Tissue: Leaf.

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Cross-references

Sequence databases
	AF093631 mRNA. Translation: AAC78103.1. AP005125 Genomic DNA. Translation: BAD30907.1.
RefSeq	NP_001059962.1.
UniGene	Os.2248
3D structure databases
HSSP	HSSP built from PDB template 1RFS based on UniProtKB P08980.
SMR	Q69S39. Positions 101-225.
ModBase	Search...
Genome annotation databases
GeneID	4343570.
KEGG	osa:4343570.
Organism-specific databases
Gramene	Q69S39.
Family and domain databases
InterPro	IPR014909. Cyt_b6-f_cplx_Fe-S_su. IPR017941. Rieske_2Fe-2S. IPR014349. Rieske_Fe-S_prot. IPR005805. Rieske_Fe-S_prot_C. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
PANTHER	PTHR10134. Rieske. 1 hit.
Pfam	PF08802. CytB6-F_Fe-S. 1 hit. PF00355. Rieske. 1 hit. [Graphical view]
PRINTS	PR00162. RIESKE.
PROSITE	PS51296. RIESKE. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

UCRIA_ORYSJ

Accession

Primary (citable) accession number: Q69S39
Secondary accession number(s): Q9ZSU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	September 13, 2004
Last modified:	October 13, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

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Relevant documents

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Family and domain databases

Entry information

Relevant documents