Q69S39 (UCRIA_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome b6-f complex iron-sulfur subunit, chloroplastic
EC=1.10.99.1

Alternative name(s):
Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein
Rieske iron-sulfur protein
Short name=ISP
Short name=RISP

Gene names

Name:	petC
Ordered Locus Names:	Os07g0556200, LOC_Os07g37030
ORF Names:	OSJNBa0058I18.25

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions By similarity.
Catalytic activity	Plastoquinol-1 + 2 oxidized plastocyanin = plastoquinone + 2 reduced plastocyanin.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity.
Subunit structure	The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are petG, petL, petM and petN. The complex functions as a dimer By similarity.
Subcellular location	Plastid › chloroplast thylakoid membrane; Single-pass membrane protein By similarity. Note: The transmembrane helix obliquely spans the membrane in one monomer, and its extrinsic C-terminal domain is part of the other monomer By similarity.
Miscellaneous	This protein is 1 of 2 subunits of the cytochrome b6-f complex that are encoded in the nucleus. The Rieske iron-sulfur protein is a high potential 2Fe-2S protein.
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Chloroplast Membrane Plastid Thylakoid
Domain	Transit peptide Transmembrane Transmembrane helix
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW plastoquinol-plastocyanin reductase activity Inferred from electronic annotation. Source: EC ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 46	46	Chloroplast Ref.3
	Chain	47 – 225	179	Cytochrome b6-f complex iron-sulfur subunit, chloroplastic		PRO_0000030690
Regions
	Transmembrane	69 – 89	21	Helical; Potential
	Domain	112 – 208	97	Rieske
Sites
	Metal binding	154	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	156	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	172	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	175	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Amino acid modifications
	Disulfide bond	159 ↔ 174		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q69S39 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: 3CB5116F297AF8B0	FASTA	225	23,884
	10 20 30 40 50 60 MASTALSTAS NPTQLCRSRA SLGKPVKGLG FGRERVPRTA TTITCQAASS IPADRVPDMG 70 80 90 100 110 120 KRQLMNLLLL GAISLPTVGM LVPYGAFFIP AGSGNAGGGQ VAKDKLGNDV LAEEWLKTHG 130 140 150 160 170 180 PNDRTLTQGL KGDPTYLVVE ADKTLATYGI NAVCTHLGCV VPWNAAENKF ICPCHGSQYN 190 200 210 220 NQGRVVRGPA PLSLALVHAD VDDGKVLFVP WVETDFRTGD NPWWA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of cytochrome B6/F-complex iron-sulfur protein in rice." Lee J.-S. Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Ilpoom. Tissue: Seedling leaf.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003." Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K. Nucleic Acids Res. 32:D388-D392(2004) [PubMed: 14681440] [Abstract] Cited for: PROTEIN SEQUENCE OF 47-56. Strain: cv. Nipponbare. Tissue: Leaf.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF093631 mRNA. Translation: AAC78103.1. AP005125 Genomic DNA. Translation: BAD30907.1.
RefSeq	NP_001059962.1. NM_001066497.1.
UniGene	Os.2248.
3D structure databases
ProteinModelPortal	Q69S39.
SMR	Q69S39. Positions 56-225.
ModBase	Search...
Protein-protein interaction databases
STRING	Q69S39.
Proteomic databases
PRIDE	Q69S39.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os07g37030.1; LOC_Os07g37030.1; LOC_Os07g37030.
GeneID	4343570.
KEGG	osa:4343570.
Organism-specific databases
Gramene	Q69S39.
Phylogenomic databases
GeneTree	EPGT00050000018070.
OMA	YPVANYF.
PhylomeDB	Q69S39.
ProtClustDB	CLSN2685934.
Family and domain databases
InterPro	IPR014909. Cyt_b6-f_cplx_Fe-S_su. IPR023960. Cyt_b6_f_Rieske. IPR017941. Rieske_2Fe-2S. IPR014349. Rieske_Fe-S_prot. IPR005805. Rieske_Fe-S_prot_C. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
KO	K02636.
PANTHER	PTHR10134. Rieske. 1 hit.
Pfam	PF08802. CytB6-F_Fe-S. 1 hit. PF00355. Rieske. 1 hit. [Graphical view]
PRINTS	PR00162. RIESKE.
SUPFAM	SSF50022. Rieske_dom. 1 hit.
PROSITE	PS51296. RIESKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UCRIA_ORYSJ

Accession

Primary (citable) accession number: Q69S39
Secondary accession number(s): Q9ZSU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	September 13, 2004
Last modified:	December 14, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program