Q69R21 (ALDO4_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable aldehyde oxidase 4
Short name=AO-4
EC=1.2.3.1

Gene names

Ordered Locus Names:	Os07g0282300, LOC_Os07g18154
ORF Names:	P0557D09.36

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	837 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	An aldehyde + H2O + O2 = a carboxylic acid + H2O2.
Cofactor	Binds 2 2Fe-2S clusters By similarity. FAD By similarity. Molybdopterin By similarity.
Subunit structure	Aldehyde oxidases (AO) are homo- and heterodimers of AO subunits By similarity.
Sequence similarities	Belongs to the xanthine dehydrogenase family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Abscisic acid biosynthesis Auxin biosynthesis
Ligand	2Fe-2S FAD Flavoprotein Iron Iron-sulfur Metal-binding Molybdenum NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	abscisic acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW auxin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW aldehyde oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on CH-OH group of donors Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 837	837	Probable aldehyde oxidase 4		PRO_0000247649
Regions
	Domain	9 – 98	90	2Fe-2S ferredoxin-type
	Domain	240 – 427	188	FAD-binding PCMH-type
Sites
	Metal binding	50	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	55	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	58	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	80	1	Iron-sulfur (2Fe-2S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q69R21 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: 7F4AC008786D0B49	FASTA	837	91,370
	10 20 30 40 50 60 MTKVVAPVER VVFELNGERQ EVAAADVEPS TTLLEFIRTR TPFRGPKLGC GEGGCGACVI 70 80 90 100 110 120 LIAKYNPKTD EVTEFNVNSC LTLLYSIHFC SIITTEGLGN TKDGFHSIQK RMSGFHASQC 130 140 150 160 170 180 GFCTPGMCMS IFSSLVNADK SKKPAPPKGF SKLSISEAER SFSGNMCRCT GYRPIVDACK 190 200 210 220 230 240 SFESDVDLED LGLNIFWKKG DKHPDPTKLP SYTLGGGICT FPDFLKSEIK SSLDFNDASI 250 260 270 280 290 300 SGPREGWYCP KSIKQYYKLV NSGLFSESSV KVVVGNTSTG VYKDQDLYDK YIDIAGIPEL 310 320 330 340 350 360 SAIVRKDKGI EIGAATSISR TIEILNQESE LTSSPNGSVV FRKLAEHMSK VASPFVRNTA 370 380 390 400 410 420 SIGGNIILAH KYPFRSDIAT ILLGAAATVN LQVSSKTLHV NLEQFLEQPP LDHSTLLLSI 430 440 450 460 470 480 FIPHWASDCK KEHTLVFETY RAAPRPLGNA VSYVNSAFLG HVSLDKSSGD NILSNLHLAF 490 500 510 520 530 540 GAYGTKHAIR ARKVEEYLTG KILSASVVLE AIRLLRETIV PVEGTTHPEY RVSVAVGFLF 550 560 570 580 590 600 SFLSPLCKGV IESGKTLSIS EDLVDTDNVH NKPLSSRRET LSDDEYTPVG DPIKKYKVEV 610 620 630 640 650 660 QASGEAIYVD DIPAPKNCLY GEFIYSTQPL ANVKSIKFKP SLASKKIITV VSAKDIPTGG 670 680 690 700 710 720 RNIGSTFWFG DEEPLFGDPI AEFAGQVLGV VIAETQPYAD MAAKQAVVEY TTDGLKAPIL 730 740 750 760 770 780 TVEQAVQSNS YFQVPPERAP KQVGDFSNGM AEADHKIMSE EVKLSSQYYF YMETQTALAI 790 800 810 820 830 PDEDNTMTVY SSSQFSELAQ NVISKCLGIP FNNVRVITRR AGGGFGGKVV RSLHVRI

References

[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP005260 Genomic DNA. Translation: BAD31252.1. AK072847 mRNA. No translation available.
3D structure databases
ProteinModelPortal	Q69R21.
ModBase	Search...
Protein-protein interaction databases
STRING	Q69R21.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	Q69R21.
Phylogenomic databases
GeneTree	EPGT00050000011134.
Family and domain databases
InterPro	IPR002888. 2Fe-2S-bd. IPR006058. 2Fe2S_fd_BS. IPR000674. Ald_Oxase/Xan_DH_a/b. IPR008274. AldOxase/xan_DH_Mopterin-bd. IPR012675. Beta-grasp_ferredoxin-type. IPR005107. CO_DH_flav_C. IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR001041. Ferredoxin. IPR002346. Mopterin_DH_FAD-bd. [Graphical view]
Gene3D	G3DSA:1.10.150.120. 2Fe-2S-bd. 1 hit. G3DSA:3.30.365.10. Ald_xan_DH_mo_bd. 1 hit. G3DSA:3.90.1170.50. Aldxan_DH_hamm. 1 hit. G3DSA:3.30.390.50. CO_DH_flav_C. 1 hit. G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF01315. Ald_Xan_dh_C. 1 hit. PF02738. Ald_Xan_dh_C2. 1 hit. PF03450. CO_deh_flav_C. 1 hit. PF00941. FAD_binding_5. 1 hit. PF00111. Fer2. 1 hit. PF01799. Fer2_2. 1 hit. [Graphical view]
SMART	SM01008. Ald_Xan_dh_C. 1 hit. SM01092. CO_deh_flav_C. 1 hit. [Graphical view]
SUPFAM	SSF47741. 2Fe-2S_bind. 1 hit. SSF56003. Ald_xan_DH_mo_bd. 1 hit. SSF54665. Aldxan_dh_hamm. 1 hit. SSF55447. CO_deh_flav_C. 1 hit. SSF56176. FAD-binding_2. 1 hit. SSF54292. Ferredoxin. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. 1 hit. PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALDO4_ORYSJ

Accession

Primary (citable) accession number: Q69R21

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	September 13, 2004
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program