Reviewed, UniProtKB/Swiss-Prot Q69NF5 (GUN23_ORYSJ)

Names and origin

Protein names

Recommended name:
    Endoglucanase 23
    EC=3.2.1.4
Alternative name(s):
    Endo-1,4-beta glucanase 23
    OsGLU12

Gene names

Name:	GLU12
Ordered Locus Names:	Os09g0530200, LOC_Os09g36060
ORF Names:	OJ1531_B07.26

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	515 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 515	486	Endoglucanase 23		PRO_0000249300
Sites
	Active site	426	1	By similarity
	Active site	477	1	By similarity
	Active site	486	1	By similarity
Amino acid modifications
	Glycosylation	178	1	N-linked (GlcNAc...) Potential
	Glycosylation	375	1	N-linked (GlcNAc...) Potential
	Glycosylation	384	1	N-linked (GlcNAc...) Potential
	Glycosylation	452	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q69NF5-1 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: A694BD5C4401C259	FASTA	515	55,453
	10 20 30 40 50 60 MALLSAPVRR RRSRVRVLLV CCCLLLALAA PSAAAAAAGH DYGDALAKSI LFFEGQRSGR 70 80 90 100 110 120 LPAAGQRAAW RGDSAVSDGG AAGVDLEGGY YDAGDNVKFG FPMAFTATML AWGVVEFGDA 130 140 150 160 170 180 MPPAERAHAA DAVRWATDYL LKTISHPGVI FIQVGDPTKD HGCWERPEDM DTARTVYNIS 190 200 210 220 230 240 AARPGSDVAG ETAAALAAAS MVFRDDDPAY AARLLAGARS AFEFADEHKG AYSDDPELRA 250 260 270 280 290 300 GGCPFYCDFD GYQDELLWGA AWLRRASKEG TYLDYIQNNG KTLGAEDSTN EFGWDNKHAG 310 320 330 340 350 360 INVLVSKEFI DGEVLSLQSY KEFADGFICT LIPESSSPHI TYTPGGMIYK PGGSNMQHVT 370 380 390 400 410 420 SISFLLLTYA KYLSNSSRTV NCGNVSVGPA TLQQLARKQA DYILGDNPMK MSYMVGYGDR 430 440 450 460 470 480 YPQRIHHRGS SLPSIKSHPQ RIACNDGTPY YNSSSPNPNP LIGAVVGGPG EDDVYEDDRA 490 500 510 DFRKSEPTTY INAPLVGVLA YLVGNPDPGQ GHVRH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP005682 Genomic DNA. Translation: BAD33772.1. AP008215 Genomic DNA. Translation: BAF25661.1. AK106815 mRNA. Translation: BAG97842.1.
RefSeq	NP_001063747.1.
UniGene	Os.54812
3D structure databases
HSSP	HSSP built from PDB template 1TF4 based on UniProtKB P26221.
ModBase	Search...
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Genome annotation databases
GeneID	4347660.
GenomeReviews	Gene locus GLU12 in contig AP008215_GR.
KEGG	osa:4347660.
Organism-specific databases
Gramene	Q69NF5.
Phylogenomic databases
HOGENOM	HBG746347.
Family and domain databases
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Gene3D	G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
PANTHER	PTHR22298:SF3. Glyco_hydro_9. 1 hit.
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN23_ORYSJ

Accession

Primary (citable) accession number: Q69NF5
Secondary accession number(s): Q0J054

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	September 13, 2004
Last modified:	December 15, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)