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Q69LE7

- PNP1_ORYSJ

UniProt

Q69LE7 - PNP1_ORYSJ

Protein

Probable polyribonucleotide nucleotidyltransferase 1, chloroplastic

Gene

PNP1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (25 Oct 2004)
      Previous versions | rss
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    Functioni

    Involved in the metabolism of all major classes of plastid RNAs. Required for efficient 3'-end processing of mRNAs and 3'-end maturation of rRNA transcripts, but is not sufficient to mediate their degradation. Mediates tRNA degradation. May function as a poly(A) mRNA 3'-5' degrading phosphorylase By similarity.By similarity

    Catalytic activityi

    RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.

    GO - Molecular functioni

    1. 3'-5'-exoribonuclease activity Source: InterPro
    2. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-EC
    3. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. carotene biosynthetic process Source: EnsemblPlants/Gramene
    2. cellular response to phosphate starvation Source: EnsemblPlants/Gramene
    3. chlorophyll biosynthetic process Source: EnsemblPlants/Gramene
    4. chloroplast RNA processing Source: EnsemblPlants/Gramene
    5. mRNA catabolic process Source: InterPro
    6. mRNA processing Source: UniProtKB-KW
    7. negative regulation of isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway Source: EnsemblPlants/Gramene
    8. rRNA processing Source: UniProtKB-KW
    9. tRNA processing Source: UniProtKB-KW
    10. xanthophyll biosynthetic process Source: EnsemblPlants/Gramene

    Keywords - Molecular functioni

    Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    mRNA processing, rRNA processing, tRNA processing

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable polyribonucleotide nucleotidyltransferase 1, chloroplastic (EC:2.7.7.8)
    Alternative name(s):
    Polynucleotide phosphorylase 1
    Short name:
    PNPase 1
    Gene namesi
    Name:PNP1
    Ordered Locus Names:Os07g0168000, LOC_Os07g07310
    ORF Names:OSJNBa0050F10.4
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 7

    Organism-specific databases

    GrameneiQ69LE7.

    Subcellular locationi

    Plastidchloroplast By similarity

    GO - Cellular componenti

    1. chloroplast stroma Source: EnsemblPlants/Gramene

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 6666ChloroplastSequence AnalysisAdd
    BLAST
    Chaini67 – 902836Probable polyribonucleotide nucleotidyltransferase 1, chloroplasticPRO_0000420276Add
    BLAST

    Proteomic databases

    PRIDEiQ69LE7.

    Interactioni

    Protein-protein interaction databases

    STRINGi39947.LOC_Os07g07310.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ69LE7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini693 – 75361KHAdd
    BLAST
    Domaini763 – 83270S1 motifAdd
    BLAST

    Sequence similaritiesi

    Contains 1 KH domain.Curated
    Contains 1 S1 motif domain.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    HOGENOMiHOG000218327.
    KOiK00962.
    OMAiEIYRNCE.

    Family and domain databases

    Gene3Di1.10.10.400. 2 hits.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPiMF_01595. PNPase.
    InterProiIPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view]
    PANTHERiPTHR11252. PTHR11252. 1 hit.
    PfamiPF00013. KH_1. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view]
    SMARTiSM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
    PROSITEiPS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q69LE7-1 [UniParc]FASTAAdd to Basket

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    MLATPGALHH LLLLPPPPHT QLAFHHAVGG VPAALLPLPR PRRVAASAST    50
    SRRGGARRRA AGARVRASVG EEAPPVVTEE ASTSGGPTKF STKIPVGDRH 100
    ILVETGHIGR QASASVMVTD GETIVYSSVC LADTPNDPSD FFPMSVHYQE 150
    RLSAAGRTSG GFFKREGRAK DHEVLVCRLI DRPLRPTMPK GFYYETQILS 200
    WVFSYDGIHS PDSLAITAAG VAMALSEVPN KQTIAGVRVG MINDQFVVNP 250
    TTEQMDDSEL DLVMAGTDSA ILMIEGYCDF LTEEKLLQAV ETGQGAIREI 300
    CKAIDGLVQK CGKKKMFDAI DLPPPELYRH VEDISGDELV KALQIKEKIL 350
    RRKALSALEE KVITILSEQG YVAKDESSGV SENLADVIEE EDEDEVIVDG 400
    EVDEGEVHIK PVSRKPPRQL FSEVDVKLVF KEVSSKFLRR RIVEGGKRSD 450
    GRSPCELRPI NSQCGLLPRA HGSALFTRGE TQALAVVTLG DYQMAQRIDN 500
    LVDTEESKSF YLQYTFPPSS VGEVGRIGAP NRREIGHGML AERALEPILP 550
    PEEDFPYTIR VESTITESNG SSSMASVCGG CLALQDAGVP IKFPVAGIAM 600
    GLVLDTLEFG GDGKPLILSD ITGAEDASGD MDFKVAGNEN GISAFQMDIK 650
    VVGITLPIME HALLQARDGR KHILNEMSKC SPPPAKVLSP YAPLIHVMKV 700
    KPNKVNLIIG SGGKTIKSII EETGVDAIDT GDDGTVKITA RDLSSLEKSK 750
    AIIANLTMVP KVGEIYRNCE IKTIAPYGAF VEIAPGREGL CHISELSSSW 800
    LAKAEDAFKV GDRIDVKLIE INDKGQLRLS SRALLPDANQ ESSSKQQAGG 850
    STREKAPQKD NLVKMTTRRP RRKKQAEAST AENNATASPK DLASQGSEMG 900
    TE 902
    Length:902
    Mass (Da):97,400
    Last modified:October 25, 2004 - v1
    Checksum:i1635BFD20D871DA6
    GO

    Sequence cautioni

    The sequence AK065622 differs from that shown. Reason: Sequencing errors.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP005840 Genomic DNA. Translation: BAD31776.1.
    AP008213 Genomic DNA. Translation: BAF20896.1.
    AK065622 mRNA. No translation available.
    RefSeqiNP_001058982.1. NM_001065517.1.
    UniGeneiOs.23950.

    Genome annotation databases

    EnsemblPlantsiOS07T0168000-01; OS07T0168000-01; OS07G0168000.
    GeneIDi4342497.
    KEGGiosa:4342497.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP005840 Genomic DNA. Translation: BAD31776.1 .
    AP008213 Genomic DNA. Translation: BAF20896.1 .
    AK065622 mRNA. No translation available.
    RefSeqi NP_001058982.1. NM_001065517.1.
    UniGenei Os.23950.

    3D structure databases

    ProteinModelPortali Q69LE7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 39947.LOC_Os07g07310.1.

    Proteomic databases

    PRIDEi Q69LE7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS07T0168000-01 ; OS07T0168000-01 ; OS07G0168000 .
    GeneIDi 4342497.
    KEGGi osa:4342497.

    Organism-specific databases

    Gramenei Q69LE7.

    Phylogenomic databases

    HOGENOMi HOG000218327.
    KOi K00962.
    OMAi EIYRNCE.

    Family and domain databases

    Gene3Di 1.10.10.400. 2 hits.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPi MF_01595. PNPase.
    InterProi IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view ]
    PANTHERi PTHR11252. PTHR11252. 1 hit.
    Pfami PF00013. KH_1. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view ]
    SMARTi SM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
    PROSITEi PS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    2. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    3. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Nipponbare.

    Entry informationi

    Entry nameiPNP1_ORYSJ
    AccessioniPrimary (citable) accession number: Q69LE7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2012
    Last sequence update: October 25, 2004
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3