Reviewed, UniProtKB/Swiss-Prot Q69L99 (LAC14_ORYSJ)

Names and origin

Protein names

Recommended name:
    Laccase-14
    EC=1.10.3.2
Alternative name(s):
    Benzenediol:oxygen oxidoreductase 14
    Urishiol oxidase 14
    Diphenol oxidase 14

Gene names

Name:	LAC14
Ordered Locus Names:	Os07g0101000, LOC_Os07g01110
ORF Names:	B1026C12.14, OsJ_021873

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	583 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Lignin degradation and detoxification of lignin-derived products By similarity.
Catalytic activity	4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O.
Cofactor	Binds 4 copper ions per monomer By similarity.
Subcellular location	Secreted › extracellular space › apoplastPotential.
Sequence similarities	Belongs to the multicopper oxidase family. Contains 3 plastocyanin-like domains.

Ontologies

Keywords
Biological process	Lignin degradation
Cellular component	Apoplast Secreted
Domain	Repeat Signal
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	copper ion binding Inferred from electronic annotation. Source: InterPro laccase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Potential
	Chain	31 – 583	553	Laccase-14		PRO_0000291898
Regions
	Domain	38 – 158	121	Plastocyanin-like 1
	Domain	168 – 320	153	Plastocyanin-like 2
	Domain	426 – 567	142	Plastocyanin-like 3
Sites
	Metal binding	88	1	Copper 1 By similarity
	Metal binding	90	1	Copper 2 By similarity
	Metal binding	137	1	Copper 2 By similarity
	Metal binding	139	1	Copper 3 By similarity
	Metal binding	482	1	Copper 4 By similarity
	Metal binding	485	1	Copper 1 By similarity
	Metal binding	487	1	Copper 3 By similarity
	Metal binding	546	1	Copper 3 By similarity
	Metal binding	547	1	Copper 4 By similarity
	Metal binding	548	1	Copper 2 By similarity
	Metal binding	552	1	Copper 4 By similarity
Amino acid modifications
	Glycosylation	41	1	N-linked (GlcNAc...) Potential
	Glycosylation	84	1	N-linked (GlcNAc...) Potential
	Glycosylation	126	1	N-linked (GlcNAc...) Potential
	Glycosylation	179	1	N-linked (GlcNAc...) Potential
	Glycosylation	251	1	N-linked (GlcNAc...) Potential
	Glycosylation	304	1	N-linked (GlcNAc...) Potential
	Glycosylation	338	1	N-linked (GlcNAc...) Potential
	Glycosylation	388	1	N-linked (GlcNAc...) Potential
	Glycosylation	400	1	N-linked (GlcNAc...) Potential
	Glycosylation	446	1	N-linked (GlcNAc...) Potential
	Glycosylation	464	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q69L99-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 68AFA7E109F0A882	FASTA	583	63,802
	10 20 30 40 50 60 MAPSLGSGST RILLIVSLLL CLRQQAVVDA AIVEHTFHVG NLTVERLGQR QVITAVNGQF 70 80 90 100 110 120 PGPKVEARNG DTLLVRVVNN SPYNITIHWH GVLQRLSAWA DGPAMVTQCP ILPGSGAGSS 130 140 150 160 170 180 YTYRFNVTGQ EGTLWWHAHV SFLRATVYGA LLIRPRPGVP YPFPAPHAEH TLLLGEWWNA 190 200 210 220 230 240 SATLVDVERQ AFLTGGQPAN SVALTINGMP GLSHAHKEMH HLRVARGNTY LLRLVNAALN 250 260 270 280 290 300 YQLFFKVAAH NFTVVAVDAC YTDPYHTDVI VIAPGQTVDA LMHAGAAPGR RYYVAAQVYQ 310 320 330 340 350 360 SIANATYSAT ARALLRYDDD AKDAAKTIIM SPRMPVLNDS ATAQRFYGSL TGLLRDGKPT 370 380 390 400 410 420 VPQRVDTRMV VTYGLAIAPC LPAQTLCNRT RGSLAASMNN VSFQLPATMS LLEASRSRSS 430 440 450 460 470 480 GVYTRDFPDR PPVMFDFTNA AAVNRNMSLM VTSKGTRVKA LRYNETVEVV LQNTAVLGTE 490 500 510 520 530 540 NHPLHLHGFN FYVLAQGTGN YYYLIRKKKI RKNLVNPQQR NTIAVPPGGW AVIRFTADNP 550 560 570 580 GVWLMHCHLE AHLPFGLAMA FDVQDGPTPD AMLPPPPNDY PPC

References

[1]

"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

[2]

"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP005869 Genomic DNA. Translation: BAD31823.1. CM000144 Genomic DNA. Translation: EAZ38390.1.
3D structure databases
HSSP	HSSP built from PDB template 1AOZ based on UniProtKB P37064.
ModBase	Search...
Organism-specific databases
Gramene	Q69L99.
Family and domain databases
InterPro	IPR001117. Cu-oxidase. IPR011706. Cu-oxidase_2. IPR011707. Cu-oxidase_3. IPR002355. Cu_oxidase_Cu_BS. IPR008972. Cupredoxin. IPR017761. Laccase. [Graphical view]
Gene3D	G3DSA:2.60.40.420. Cupredoxin. 3 hits.
Pfam	PF00394. Cu-oxidase. 1 hit. PF07731. Cu-oxidase_2. 1 hit. PF07732. Cu-oxidase_3. 1 hit. [Graphical view]
PROSITE	PS00079. MULTICOPPER_OXIDASE1. False negative. PS00080. MULTICOPPER_OXIDASE2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LAC14_ORYSJ

Accession

Primary (citable) accession number: Q69L99

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	October 25, 2004
Last modified:	November 25, 2008
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)