Reviewed, UniProtKB/Swiss-Prot Q69BK3 (UCRI_PONPY)

Names and origin

Protein names

Recommended name:
    Cytochrome b-c1 complex subunit Rieske, mitochondrial
    EC=1.10.2.2
Alternative name(s):
    Ubiquinol-cytochrome c reductase iron-sulfur subunit
    Rieske iron-sulfur protein
      Short name=RISP
    Cytochrome b-c1 complex subunit 5
    Complex III subunit 5
Cleaved into the following chain:
    1- Recommended name:
            Cytochrome b-c1 complex subunit 11
        Alternative name(s):
            Ubiquinol-cytochrome c reductase 8 kDa protein
            Complex III subunit IX

Gene names

Name:

UQCRFS1

Organism

Pongo pygmaeus (Bornean orangutan)

Taxonomic identifier

9600 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis By similarity. The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex By similarity.
Catalytic activity	QH2 + 2 ferricytochrome c = Q + 2 ferrocytochrome c + 2 H+.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity.
Subunit structure	The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1) By similarity.
Subcellular location	Mitochondrion inner membrane By similarity.
Miscellaneous	The Rieske protein is a high potential 2Fe-2S protein.
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide Transmembrane
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 78	78	Cytochrome b-c1 complex subunit 11 By similarity		PRO_0000307247
	Transit peptide	1 – 78	78	Mitochondrion By similarity
	Chain	79 – 274	196	Cytochrome b-c1 complex subunit Rieske, mitochondrial		PRO_0000030670
Regions
	Domain	187 – 272	86	Rieske
Sites
	Metal binding	217	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	219	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	236	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	239	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Amino acid modifications
	Disulfide bond	222 ↔ 238		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q69BK3-1 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: 809FF6E251E0F4B2	FASTA	274	29,765
	10 20 30 40 50 60 MLSVATRSGP FAPVLSATSR GVAGALRPLV QATVPATPEQ PVLNLKRPFL SRESLSGQAV 70 80 90 100 110 120 RRPLVASVGL NVPASVCYSH TDVRVPDFSE YRRLEVLDST KSSRESSEAR KGFSYLVTGV 130 140 150 160 170 180 TTVGVAYAAK NVVTQFVSSM SASADVLALA KIEIKLSDIP EGKNMTFKWR GKPLFVRHRT 190 200 210 220 230 240 QKEIEQEAAV ELSQLRDPQH DLDRVKKPEW VILIGVCTHL GCVPIANAGD FGGYYCPCHG 250 260 270 SHYDASGRIR LGPAPLNLEV PIYEFTSDDM VIVG

References

[1]

"Molecular evolution of the iron sulfur protein and subunit 9 of complex III of the electron transport chain in primates." Doan J.W., Wildman D.E., Schmidt T.R., Weiss M.L., Goodman M., Grossman L.I. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY387502, AY387501 Genomic DNA. Translation: AAR32733.1.
3D structure databases
SMR	Q69BK3. Positions 1-57, 79-274.
ModBase	Search...
Genome annotation databases
Ensembl	ENSPPYT00000011408; ENSPPYP00000010979; ENSPPYG00000009804; Pongo pygmaeus. [Genome view]
Phylogenomic databases
HOVERGEN	Q69BK3.
InParanoid	Q69BK3.
Enzyme and pathway databases
BRENDA	1.10.2.2. 269192.
Family and domain databases
InterPro	IPR011070. Globular_prot_asu/bsu. IPR017941. Rieske_2Fe-2S. IPR014349. Rieske_Fe-S_prot. IPR005805. Rieske_Fe-S_prot_C. IPR015248. Ubiqinol_cyt_c_Rdtase_N. IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su. IPR004192. Ubiquinol_cyt_Rdtase_TM. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
PANTHER	PTHR10134. Rieske. 1 hit.
Pfam	PF00355. Rieske. 1 hit. PF09165. Ubiq-Cytc-red_N. 1 hit. PF02921. UCR_TM. 1 hit. [Graphical view]
PRINTS	PR00162. RIESKE.
TIGRFAMs	TIGR01416. Rieske_proteo. 1 hit.
PROSITE	PS51296. RIESKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UCRI_PONPY

Accession

Primary (citable) accession number: Q69BK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	September 13, 2004
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)