Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Zinc metalloproteinase/disintegrin

Gene
N/A
Organism
Gloydius brevicaudus (Korean slamosa snake) (Agkistrodon halys brevicaudus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Snake venom metalloproteinase brevilysin L4: metalloproteinase that impairs hemostasis in the envenomed animal (By similarity). Shows autoproteolysis dependent on pH and temperature. Does not show hemorrhagic activity.By similarity2 Publications
Disintegrin brevicaudin-1a: inhibits platelet aggregation induced by ADP (IC50 is 200 nM), collagen (IC50 is 500 nM), thrombin and epinephrin (IC50 is 300 nM). Does not inhibit aggregation induced by ristocetin.
Disintegrin brevicaudin-1b: inhibits platelet aggregation induced by ADP (IC50 is 100 nM), collagen (IC50 is 500 nM), thrombin and epinephrin (IC50 is 300 nM). Does not inhibit aggregation induced by ristocetin. Significantly inhibits angiogenesis both in vivo and in vitro.

Miscellaneous

The disintegrins belong to the medium disintegrin subfamily.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Excess of calcium ions significantly suppress the autoproteolysis of the enzyme.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi37CalciumBy similarity1
Metal bindingi121CalciumBy similarity1
Metal bindingi170Zinc; catalyticBy similarity1
Active sitei171PROSITE-ProRule annotation1
Metal bindingi174Zinc; catalyticBy similarity1
Metal bindingi180Zinc; catalyticBy similarity1
Metal bindingi225Calcium; via carbonyl oxygenBy similarity1
Metal bindingi228CalciumBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionCell adhesion impairing toxin, Hemostasis impairing toxin, Hydrolase, Metalloprotease, Platelet aggregation inhibiting toxin, Protease, Toxin
LigandCalcium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc metalloproteinase/disintegrin
Cleaved into the following 3 chains:
Alternative name(s):
Snake venom metalloproteinase hxl-1 (EC:3.4.24.-)
Alternative name(s):
Disintegrin adinbitor
Disintegrin halystatin
OrganismiGloydius brevicaudus (Korean slamosa snake) (Agkistrodon halys brevicaudus)
Taxonomic identifieri259325 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeGloydius

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_0000424444‹1 – 28By similarityAdd BLAST›28
ChainiPRO_000042444529 – 230Snake venom metalloproteinase brevilysin L4Add BLAST202
PropeptideiPRO_0000424446231 – 246By similarityAdd BLAST16
ChainiPRO_0000318180247 – 319Disintegrin brevicaudin-1bAdd BLAST73
ChainiPRO_0000424447249 – 319Disintegrin brevicaudin-1aAdd BLAST71

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi145 ↔ 225By similarity
Disulfide bondi185 ↔ 209By similarity
Disulfide bondi187 ↔ 192By similarity
Disulfide bondi252 ↔ 261By similarity
Disulfide bondi254 ↔ 262By similarity
Disulfide bondi267 ↔ 281By similarity
Disulfide bondi275 ↔ 305By similarity
Disulfide bondi280 ↔ 284By similarity
Disulfide bondi293 ↔ 312By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

SMRiQ698K8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 230Peptidase M12BPROSITE-ProRule annotationAdd BLAST197
Domaini238 – 319DisintegrinPROSITE-ProRule annotationAdd BLAST82

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi297 – 299Cell attachment site3

Sequence similaritiesi

Family and domain databases

CDDicd04269. ZnMc_adamalysin_II_like. 1 hit.
Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiView protein in InterPro
IPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
IPR036436. Disintegrin_dom_sf.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR034027. Reprolysin_adamalysin.
PfamiView protein in Pfam
PF00200. Disintegrin. 1 hit.
PF01421. Reprolysin. 1 hit.
PRINTSiPR00289. DISINTEGRIN.
SMARTiView protein in SMART
SM00050. DISIN. 1 hit.
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiView protein in PROSITE
PS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q698K8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
EDEAPKMCGV TQNWESYEPI KKASQSNLTP AHQRYIELVI VADHGMFTKY
60 70 80 90 100
NGDSDKIREW VRQMVNTVDE IYSYMYIDVA LAGLEIWSNE DLINVQPAAP
110 120 130 140 150
HTLDSFGKWR ERDLLHRIHH DNAMLLTAID FDGPTIGLAY VGTMCKPKGS
160 170 180 190 200
TGVVQDHSTI NLRVAVTMAH EIGHNLGIHH DTGSCSCGGY SCIMSPVISH
210 220 230 240 250
EPSKYFSDCS YTQCWDFIMN QKPQCILNKP LRTDTVSTPV SGNELLEAGE
260 270 280 290 300
ECDCGSPGNP CCDAATCKLR QGAQCAEGLC CDQCRFMKKG TVCRIARGDD
310
MDDYCNGISA GCPRNPFHA
Length:319
Mass (Da):35,314
Last modified:November 13, 2013 - v3
Checksum:i093F991FA589B1FA
GO

Sequence cautioni

Q698K8: The sequence AAT76292 differs from that shown. Reason: Erroneous termination at position 320. Translated as stop.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11
Sequence conflicti244 – 246ELL → MHM in AAT76292 (PubMed:15188058).Curated3

Mass spectrometryi

Q698K8: Molecular mass is 22752 Da from positions 29 - 230. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY551929 mRNA. Translation: AAT76292.1. Different termination.
PIRiA59410.
A59411.

Similar proteinsi

Entry informationi

Entry nameiVM2L4_GLOBR
AccessioniPrimary (citable) accession number: Q698K8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2013
Last modified: October 25, 2017
This is version 59 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Caution

The metalloprotease is also encoded by another precursor (AC Q90WC0).Curated

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families