Q68XZ4 (LPXD_RICTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	RT0008

Organism

Rickettsia typhi (strain ATCC VR-144 / Wilmington) [Complete proteome] [HAMAP]

Taxonomic identifier

257363 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_0000059700
Sites
	Active site	253	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q68XZ4 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 4C4C181BEDDE15F7	FASTA	346	37,251
	10 20 30 40 50 60 MVSSNFYKNL GPRKLTAIID FLHDIIEPTK IHEDIDIHDI KILQEASPND ISFLSNPKYS 70 80 90 100 110 120 EFLKTTKAAA CIVPKDFTEE SNQNTVLIHA ENSYFAYSKL IDFFYAPIKS YSTKIMKSAI 130 140 150 160 170 180 IADSAAIGKN CYIGHNVVIE DDVIIGDNSI IDAGTFIGRG VNIGKNARIE QHVSINYAII 190 200 210 220 230 240 GDDVVILVGA KIGQDGFGFA TEKGVHNKIF HIGIVKIGNN VEIGSNTTID RGALQDTIIE 250 260 270 280 290 300 DLCRIDNLVQ IGHGVKIGKG SIIVAQAGIA GSSAIGKYCA LGGQVGIAGH LNIGDRTQVA 310 320 330 340 AQGGVAQNIE EGKIVGGSPA VPIMDWHRQS IIMKQLVKTS NNKLKK

References

[1]

"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae." McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. , Hong C., Yu X.-J., Walker D.H., Weinstock G.M. J. Bacteriol. 186:5842-5855(2004) [PubMed: 15317790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017197 Genomic DNA. Translation: AAU03498.1.
RefSeq	YP_066980.1. NC_006142.1.
3D structure databases
ProteinModelPortal	Q68XZ4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2958512.
GenomeReviews	Gene locus RT0008 in contig AE017197_GR.
KEGG	rty:RT0008.
PATRIC	17909166. VBIRicTyp34752_0008.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG469615.
OMA	SYPAKIM.
ProtClustDB	PRK00892.
Enzyme and pathway databases
BioCyc	RTYP257363:RT0008-MONOMER.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 3 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_RICTY

Accession

Primary (citable) accession number: Q68XZ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program