Reviewed, UniProtKB/Swiss-Prot Q68XY7 (RUVC_RICTY)

Names and origin

Protein names

Recommended name:
    Crossover junction endodeoxyribonuclease ruvC
    EC=3.1.22.4
Alternative name(s):
    Holliday junction nuclease ruvC
    Holliday junction resolvase ruvC

Gene names

Name:	ruvC
Ordered Locus Names:	RT0017

Organism

Rickettsia typhi [Complete proteome] [HAMAP]

Taxonomic identifier

785 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	157 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity.
Catalytic activity	Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP MF_00034
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Sequence similarities	Belongs to the ruvC family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP
Molecular function	crossover junction endodeoxyribonuclease activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 157	157	Crossover junction endodeoxyribonuclease ruvC HAMAP MF_00034		PRO_0000225173
Sites
	Metal binding	7	1	Magnesium By similarity
	Metal binding	67	1	Magnesium By similarity
	Metal binding	140	1	Magnesium By similarity
	Metal binding	143	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q68XY7-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 57623699B9F09994	FASTA	157	17,434
	10 20 30 40 50 60 MIVLGIDPAL GNLGWALVAK EYTKLKYLAS GTIKTHSKDE IHNRLAFINS TLEKVILKYQ 70 80 90 100 110 120 PNIAAIEETF INTNSVTSLK LGYARGAIMS LIGRYNLNMQ EFKPNTVKKT VTGYGHAKKE 130 140 150 QILYMIKHLI PGTDLITNSD EADAVALAYT SLVTKKY

References

[1]

"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae." McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. , Hong C., Yu X.-J., Walker D.H., Weinstock G.M. J. Bacteriol. 186:5842-5855(2004) [PubMed: 15317790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017197 Genomic DNA. Translation: AAU03505.1.
RefSeq	YP_066987.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2958585.
GenomeReviews	Gene locus RT0017 in contig AE017197_GR.
KEGG	rty:RT0017.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q68XY7.
OMA	GCIRTGS
Enzyme and pathway databases
BioCyc	RTYP257363:RT0017-MON.
BRENDA	3.1.22.4. 281221.
Family and domain databases
HAMAP	MF_00034. [Tree]
InterPro	IPR012337. PolynucTfrase_RNaseH_fold. IPR020563. X-over_junc_endoDNase_Mg_BS. IPR002176. X-over_junc_endoDNase_RuvC. [Graphical view]
Pfam	PF02075. RuvC. 1 hit. [Graphical view]
PRINTS	PR00696. RSOLVASERUVC.
TIGRFAMs	TIGR00228. ruvC. 1 hit.
PROSITE	PS01321. RUVC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RUVC_RICTY

Accession

Primary (citable) accession number: Q68XY7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	October 11, 2004
Last modified:	November 24, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)