Reviewed, UniProtKB/Swiss-Prot Q68XY3 (NUOF_RICTY)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit F
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit F
    NDH-1 subunit F

Gene names

Name:	nuoF
Ordered Locus Names:	RT0021

Organism

Rickettsia typhi [Complete proteome] [HAMAP]

Taxonomic identifier

785 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Cofactor	Binds 1 FMN Potential. Binds 1 4Fe-4S cluster Potential.
Sequence similarities	Belongs to the complex I 51 kDa subunit family.

Ontologies

Keywords
Ligand	4Fe-4S FMN Flavoprotein Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FMN binding Inferred from electronic annotation. Source: InterPro NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	NADH-quinone oxidoreductase subunit F		PRO_0000274789
Regions
	Nucleotide binding	54 – 63	10	NAD By similarity
	Nucleotide binding	166 – 213	48	FMN By similarity
Sites
	Metal binding	344	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	347	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	350	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	390	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q68XY3-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 94CE3D5E4D4FF978	FASTA	421	46,347
	10 20 30 40 50 60 MLKEEDKIFI NLYGQQSYDL KSSKKRGDWY NTKALLDKGR DFIIDEVKKS GLRGRGGAGF 70 80 90 100 110 120 STGMKWSFMP KNSEKPCYLV VNADESEPGT CKDRDILRFE PHKLIEGCLI ASFAIGANNC 130 140 150 160 170 180 YIYIRGEFYN EASNMQRALD EAYTEGLIGK NACGSGFDCN IYLHRGAGAY ICGEETALLE 190 200 210 220 230 240 SLEGKKGMPR LKPPFPAGFG LYGCPTTINN VESIAVVPTI LRRGASWFAA IGKPNNTGTK 250 260 270 280 290 300 IFCISGHVNK PCNIEEAMGI PLKELIEKYA GGVRGGWDNL KAIIPGGASV PLLPKSLCEV 310 320 330 340 350 360 EMDFDSLRTV GSGLGTGGII VMDRSTDIIY AIARLSKFYM HESCGQCTPC REGTGWMWRV 370 380 390 400 410 420 MMRLVNGNAK KTEIETLLNV TKEIEGHTIC ALGDAAAWPI QGLIRHFRDE IEERIKNFGI A

References

[1]

"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae." McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. , Hong C., Yu X.-J., Walker D.H., Weinstock G.M. J. Bacteriol. 186:5842-5855(2004) [PubMed: 15317790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017197 Genomic DNA. Translation: AAU03509.1.
RefSeq	YP_066991.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2958578.
GenomeReviews	Gene locus RT0021 in contig AE017197_GR.
KEGG	rty:RT0021.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q68XY3.
OMA	Q68XY3. PCNVEEE.
Enzyme and pathway databases
BioCyc	RTYP257363:RT0021-MON.
BRENDA	1.6.99.5. 281221.
Family and domain databases
InterPro	IPR001949. NADH-UbQ_OxRdtase_51KDa_CS. IPR019575. NADH-UbQ_OxRdtase_Fsu_4Fe4S-bd. IPR011537. NADH-UbQ_OxRdtase_suF. IPR011538. NADH_UbQ_OxRdtase_51KDa_su. IPR019554. Soluble_ligand_bd. [Graphical view]
Pfam	PF01512. Complex1_51K. 1 hit. PF10589. NADH_4Fe-4S. 1 hit. PF10531. SLBB. 1 hit. [Graphical view]
TIGRFAMs	TIGR01959. nuoF_fam. 1 hit.
PROSITE	PS00644. COMPLEX1_51K_1. 1 hit. PS00645. COMPLEX1_51K_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOF_RICTY

Accession

Primary (citable) accession number: Q68XY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	October 11, 2004
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)