Reviewed, UniProtKB/Swiss-Prot Q68XX9 (TRMD_RICTY)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(1)-)-methyltransferase
    EC=2.1.1.31
Alternative name(s):
    M1G-methyltransferase
    tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	RT0025

Organism

Rickettsia typhi [Complete proteome] [HAMAP]

Taxonomic identifier

785 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	234 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine. HAMAP MF_00605
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA methyltransferase trmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 234	234	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_0000060446
Regions
	Region	135 – 140	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	115	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q68XX9-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: B4EBE958F88CD428	FASTA	234	26,463
	10 20 30 40 50 60 MSILHVTILT VFPEMFPGTL GYSLAGQALH NNIWSYDIIN IRDFGLTKHK NVDDKAYGGG 70 80 90 100 110 120 DGLIMRPDVL GNVIEYALSL NHNTKIYYPS PRGSVFTQSF AKEMLKNKNI IFLCGRYEGI 130 140 150 160 170 180 DERVIAEYNV KEISVGDYIL SGGEIPTLTI LDCLIRLLPG VLINQNTLSS ESFEKDGEFQ 190 200 210 220 230 GGLECDLYTR PKIWHGRAVP SILLSGNRKL INNWRKEQSR MITKLRRPEL LKDL

References

[1]

"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae." McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. , Hong C., Yu X.-J., Walker D.H., Weinstock G.M. J. Bacteriol. 186:5842-5855(2004) [PubMed: 15317790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017197 Genomic DNA. Translation: AAU03513.1.
RefSeq	YP_066995.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2958525.
GenomeReviews	Gene locus RT0025 in contig AE017197_GR.
KEGG	rty:RT0025.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q68XX9.
OMA	HRSVDDT
Enzyme and pathway databases
BioCyc	RTYP257363:RT0025-MON.
BRENDA	2.1.1.31. 281221.
Family and domain databases
HAMAP	MF_00605. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. [Graphical view]
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. trmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_RICTY

Accession

Primary (citable) accession number: Q68XX9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	October 11, 2004
Last modified:	November 24, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)