Q68XX8 (ACKA_RICTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetate kinase
EC=2.7.2.1

Alternative name(s):
Acetokinase

Gene names

Name:	ackA
Ordered Locus Names:	RT0026

Organism

Rickettsia typhi (strain ATCC VR-144 / Wilmington) [Complete proteome] [HAMAP]

Taxonomic identifier

257363 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group › Rickettsia typhi

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction By similarity. HAMAP-Rule MF_00020
Catalytic activity	ATP + acetate = ADP + acetyl phosphate. HAMAP-Rule MF_00020
Cofactor	Mg2+. Can also accept Mn2+ By similarity.
Pathway	Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 1/2. HAMAP-Rule MF_00020
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the acetokinase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	acetyl-CoA biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway organic acid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate kinase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 385	385	Acetate kinase HAMAP-Rule MF_00020		PRO_0000107606
Regions
	Nucleotide binding	202 – 206	5	ATP By similarity
	Nucleotide binding	277 – 279	3	ATP By similarity
Sites
	Active site	144	1	Proton donor/acceptor By similarity
	Metal binding	9	1	Magnesium By similarity
	Metal binding	373	1	Magnesium By similarity
	Binding site	16	1	ATP By similarity
	Binding site	87	1	Substrate By similarity
	Site	175	1	Transition state stabilizer By similarity
	Site	235	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q68XX8 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 6226C8A5676DA515	FASTA	385	43,381
	10 20 30 40 50 60 MKDVILIANA GSSSLKISIF GIQNKKVKDK IYNIFLEKNN NKILFYINQK QESAISIKDN 70 80 90 100 110 120 AIEMMINLFE EWWTKQSALN LIATGHRIVH GGKNFNKPVI VNEKIIKELR VLIPLSPLHQ 130 140 150 160 170 180 PYNLQVLDLF FQKYKDISHI ICFDTSFHYT NPPITKAFGL PKQYYDKGIM RYGFHGLSYQ 190 200 210 220 230 240 YVSSHFKEIT MEDLPTKTII AHLGSGSSLC AIKNGLSLTS SMGFSVLDGV MMATRTGNLD 250 260 270 280 290 300 PGVVLYLINH EKMTMKEITE LLYKKSGLLG ISGESSDMRT LITNNSHDSK FAVELFVYRI 310 320 330 340 350 360 VLEIGKLIAA LEGIDCIIFT AGIGQNSAVI REMISGKLSW LGIKIDYGKN QKNEHRISTK 370 380 GSKVKVFVVP TNEELIIAEE VMKFL

References

[1]

"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae." McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. , Hong C., Yu X.-J., Walker D.H., Weinstock G.M. J. Bacteriol. 186:5842-5855(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017197 Genomic DNA. Translation: AAU03514.1.
RefSeq	YP_066996.1. NC_006142.1.
3D structure databases
ProteinModelPortal	Q68XX8.
ModBase	Search...
Protein-protein interaction databases
STRING	257363.RT0026.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAU03514; AAU03514; RT0026.
GeneID	2958568.
KEGG	rty:RT0026.
PATRIC	17909202. VBIRicTyp34752_0024.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0282.
HOGENOM	HOG000288399.
KO	K00925.
OMA	DEDRMIA.
ProtClustDB	CLSK870749.
Enzyme and pathway databases
BioCyc	RTYP257363:GJEQ-27-MONOMER.
UniPathway	UPA00340; UER00458.
Family and domain databases
HAMAP	MF_00020. Acetate_kinase.
InterPro	IPR004372. Ac/Proprionate_kinase. IPR000890. Aliphatic_acid_kin_short-chain. IPR023865. Aliphatic_acid_kinase_CS. [Graphical view]
PANTHER	PTHR21060. PTHR21060. 1 hit.
Pfam	PF00871. Acetate_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000722. Acetate_prop_kin. 1 hit.
PRINTS	PR00471. ACETATEKNASE.
TIGRFAMs	TIGR00016. ackA. 1 hit.
PROSITE	PS01075. ACETATE_KINASE_1. 1 hit. PS01076. ACETATE_KINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACKA_RICTY

Accession

Primary (citable) accession number: Q68XX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program