Reviewed, UniProtKB/Swiss-Prot Q68XW3 (ALR_RICTY)

Names and origin

Protein names

Recommended name:
    Alanine racemase
    EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	RT0042

Organism

Rickettsia typhi [Complete proteome] [HAMAP]

Taxonomic identifier

785 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity.
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family. Contains 1 RPE1 insert domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: HAMAP cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 402	402	Alanine racemase HAMAP MF_01201		PRO_0000272406
Regions
	Domain	226 – 271	46	RPE1 insert
Sites
	Active site	34	1	Proton acceptor; specific for D-alanine By similarity
	Active site	296	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	34	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q68XW3-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 3F397F6BA4EC207B	FASTA	402	45,046
	10 20 30 40 50 60 MSLCTLEINL SAIKNNYRLL QDICTTALVG AAVKANGYGI GAMQIAKALI EENCQYFFVA 70 80 90 100 110 120 TSEEGINLRK ALNNDITILV LNGVFTHDAL ELIQYNLTPV LNNLKQIEIW QKFSNLKEKI 130 140 150 160 170 180 LPCYLHFNTG LNRFGLNYDE IEQLINDRDL LKGLDLQYII SHLAASEEMD NPYNLAQLNR 190 200 210 220 230 240 FKDYLEYFPN VKASLANSGG IFLGQDYHFD LARPGAALYG LNSLIEVSYN LSYKEKFERN 250 260 270 280 290 300 TPALATTVCI NKCADVNTRL TYKVPLKGSY RLQNPVTLKA PIIHLQNLTL DSHIGYNMTF 310 320 330 340 350 360 TTKRDSIIAT LPLGYADGFS RNFSNQGEVF INSRSVPIVG RVSMDLINID VTDLPPSEVF 370 380 390 400 LGQEVEIIGN YCTPDKIASI IGTVGYEVLT SLGSRYKRKY IS

References

[1]

"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae." McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. , Hong C., Yu X.-J., Walker D.H., Weinstock G.M. J. Bacteriol. 186:5842-5855(2004) [PubMed: 15317790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017197 Genomic DNA. Translation: AAU03529.1.
RefSeq	YP_067011.1.
3D structure databases
HSSP	HSSP built from PDB template 1XFC based on UniProtKB P0A4X2.
ModBase	Search...
Genome annotation databases
GeneID	2959361.
GenomeReviews	Gene locus RT0042 in contig AE017197_GR.
KEGG	rty:RT0042.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG712172.
OMA	YCTPDKI.
Enzyme and pathway databases
BioCyc	RTYP257363:RT0042-MON.
BRENDA	5.1.1.1. 281221.
Family and domain databases
HAMAP	MF_01201. Divergent sequence. [Tree]
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. IPR005728. Rickett_RPE. [Graphical view]
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_RICTY

Accession

Primary (citable) accession number: Q68XW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	October 11, 2004
Last modified:	December 15, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)