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Q68XV7 (KDTA_RICTY) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
3-deoxy-D-manno-octulosonic acid transferase

Short name=Kdo transferase
EC=2.4.99.12
Alternative name(s):
Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase
Gene names
Name:waaA
Synonyms:kdtA
Ordered Locus Names:RT0048
OrganismRickettsia typhi (strain ATCC VR-144 / Wilmington) [Complete proteome] [HAMAP]
Taxonomic identifier257363 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group

Protein attributes

Sequence length462 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in lipopolysaccharide (LPS) biosynthesis. Catalyzes the transfer of 3-deoxy-D-manno-octulosonate (Kdo) residue(s) from CMP-Kdo to lipid IV(A), the tetraacyldisaccharide-1,4'-bisphosphate precursor of lipid A By similarity.

Catalytic activity

Lipid IV(A) + CMP-alpha-Kdo = alpha-Kdo-(2->6)-lipid IV(A) + CMP.

Pathway

Bacterial outer membrane biogenesis; LPS core biosynthesis.

Subcellular location

Cell inner membrane; Single-pass membrane protein; Cytoplasmic side By similarity.

Sequence similarities

Belongs to the glycosyltransferase group 1 family.

Contains 1 RPE1 insert domain.

Ontologies

Keywords
   Biological processLipopolysaccharide biosynthesis
   Cellular componentCell inner membrane
Cell membrane
Membrane
   DomainSignal-anchor
Transmembrane
Transmembrane helix
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processlipopolysaccharide core region biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontransferase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4624623-deoxy-D-manno-octulosonic acid transferase
PRO_0000286457

Regions

Transmembrane2 – 2221Helical; Signal-anchor; Potential
Domain47 – 9044RPE1 insert
Region308 – 3092CMP-Kdo binding By similarity
Region349 – 3513CMP-Kdo binding By similarity
Region374 – 3774CMP-Kdo binding By similarity

Sites

Active site1041Proton acceptor By similarity
Site1741Transition state stabilizer By similarity
Site2491Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
Q68XV7 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: D23F37C837B00178

FASTA46252,900
        10         20         30         40         50         60 
MMLLYYILSF ILLPVYFIII FIRLLIGKED IRRIQERFAI GKQRQNSLLD LQMSVNQEGF 

        70         80         90        100        110        120 
KVDTEHKATS YVYIHRNASL MYKLSLERSY AQSLVWIHAA SVGEVMTSLT LIHNICKLAP 

       130        140        150        160        170        180 
NVRFLITSWT NTSAKILSTK LPKIATHQFL PIDNVIFTRK FLSNWKPDLG IFIESELWPC 

       190        200        210        220        230        240 
IINEGAKHCK LLLVNARISN KSFKTWLKRK KFFQLIIKNF SKIIVQSECD LQKFNALGIS 

       250        260        270        280        290        300 
DAMNLGNIKF ANEKLLVNQE KLSKLSLHLD NRRVVVFAST HPEDEEVILP IINNLKEQFV 

       310        320        330        340        350        360 
DCYIILIPRH PERVKSILNN CKCHNLLATA KSQNDLPVLS DDIYIVDRFG EMGLFFSVAT 

       370        380        390        400        410        420 
ISFIGGSFKQ GGHNILEAAY FSNCIIFGPD MSKNTDIAKG ILQNNAAIQI KNGEDLLNTL 

       430        440        450        460 
KSLLNANNAL KLKAYRENAL KFVEHNQKIL DEYLHVIKPF LP 

« Hide

References

[1]"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae."
McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. expand/collapse author list , Hong C., Yu X.-J., Walker D.H., Weinstock G.M.
J. Bacteriol. 186:5842-5855(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017197 Genomic DNA. Translation: AAU03535.1.
RefSeqYP_067017.1. NC_006142.1.

3D structure databases

ProteinModelPortalQ68XV7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING257363.RT0048.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU03535; AAU03535; RT0048.
GeneID2958750.
KEGGrty:RT0048.
PATRIC17909246. VBIRicTyp34752_0045.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1519.
HOGENOMHOG000010628.
KOK02527.
OMALDHWQPD.
OrthoDBEOG6H7FNN.

Enzyme and pathway databases

BioCycRTYP257363:GJEQ-50-MONOMER.
UniPathwayUPA00958.

Family and domain databases

InterProIPR007507. Glycos_transf_N.
[Graphical view]
PfamPF04413. Glycos_transf_N. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKDTA_RICTY
AccessionPrimary (citable) accession number: Q68XV7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: October 11, 2004
Last modified: May 14, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia typhi

Rickettsia typhi (strain Wilmington): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways