Q68XV7 (KDTA_RICTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-deoxy-D-manno-octulosonic acid transferase
Short name=Kdo transferase
EC=2.4.99.12

Alternative name(s):
Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase

Gene names

Name:	waaA
Synonyms:	kdtA
Ordered Locus Names:	RT0048

Organism

Rickettsia typhi (strain ATCC VR-144 / Wilmington) [Complete proteome] [HAMAP]

Taxonomic identifier

257363 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group › Rickettsia typhi

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in lipopolysaccharide (LPS) biosynthesis. Catalyzes the transfer of 3-deoxy-D-manno-octulosonate (Kdo) residue(s) from CMP-Kdo to lipid IV(A), the tetraacyldisaccharide-1,4'-bisphosphate precursor of lipid A By similarity.
Catalytic activity	Lipid IV(A) + CMP-alpha-Kdo = alpha-Kdo-(2->6)-lipid IV(A) + CMP.
Pathway	Bacterial outer membrane biogenesis; LPS core biosynthesis.
Subcellular location	Cell inner membrane; Single-pass membrane protein; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the glycosyltransferase group 1 family. Contains 1 RPE1 insert domain.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	lipopolysaccharide core region biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 462	462	3-deoxy-D-manno-octulosonic acid transferase		PRO_0000286457
Regions
	Transmembrane	2 – 22	21	Helical; Signal-anchor; Potential
	Domain	47 – 90	44	RPE1 insert
	Region	308 – 309	2	CMP-Kdo binding By similarity
	Region	349 – 351	3	CMP-Kdo binding By similarity
	Region	374 – 377	4	CMP-Kdo binding By similarity
Sites
	Active site	104	1	Proton acceptor By similarity
	Site	174	1	Transition state stabilizer By similarity
	Site	249	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q68XV7 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: D23F37C837B00178	FASTA	462	52,900
	10 20 30 40 50 60 MMLLYYILSF ILLPVYFIII FIRLLIGKED IRRIQERFAI GKQRQNSLLD LQMSVNQEGF 70 80 90 100 110 120 KVDTEHKATS YVYIHRNASL MYKLSLERSY AQSLVWIHAA SVGEVMTSLT LIHNICKLAP 130 140 150 160 170 180 NVRFLITSWT NTSAKILSTK LPKIATHQFL PIDNVIFTRK FLSNWKPDLG IFIESELWPC 190 200 210 220 230 240 IINEGAKHCK LLLVNARISN KSFKTWLKRK KFFQLIIKNF SKIIVQSECD LQKFNALGIS 250 260 270 280 290 300 DAMNLGNIKF ANEKLLVNQE KLSKLSLHLD NRRVVVFAST HPEDEEVILP IINNLKEQFV 310 320 330 340 350 360 DCYIILIPRH PERVKSILNN CKCHNLLATA KSQNDLPVLS DDIYIVDRFG EMGLFFSVAT 370 380 390 400 410 420 ISFIGGSFKQ GGHNILEAAY FSNCIIFGPD MSKNTDIAKG ILQNNAAIQI KNGEDLLNTL 430 440 450 460 KSLLNANNAL KLKAYRENAL KFVEHNQKIL DEYLHVIKPF LP

References

[1]

"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae." McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. , Hong C., Yu X.-J., Walker D.H., Weinstock G.M. J. Bacteriol. 186:5842-5855(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017197 Genomic DNA. Translation: AAU03535.1.
RefSeq	YP_067017.1. NC_006142.1.
3D structure databases
ProteinModelPortal	Q68XV7.
ModBase	Search...
Protein-protein interaction databases
STRING	257363.RT0048.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAU03535; AAU03535; RT0048.
GeneID	2958750.
KEGG	rty:RT0048.
PATRIC	17909246. VBIRicTyp34752_0045.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1519.
HOGENOM	HOG000010628.
KO	K02527.
OMA	KHGGHNP.
ProtClustDB	PRK05749.
Enzyme and pathway databases
BioCyc	RTYP257363:GJEQ-50-MONOMER.
UniPathway	UPA00958.
Family and domain databases
InterPro	IPR007507. Glycos_transf_N. [Graphical view]
Pfam	PF04413. Glycos_transf_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KDTA_RICTY

Accession

Primary (citable) accession number: Q68XV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program