Q68XV7 (KDTA_RICTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-deoxy-D-manno-octulosonic-acid transferase
EC=2.4.-.-

Alternative name(s):
KDO transferase

Gene names

Name:	waaA
Synonyms:	kdtA
Ordered Locus Names:	RT0048

Organism

Rickettsia typhi (strain ATCC VR-144 / Wilmington) [Complete proteome] [HAMAP]

Taxonomic identifier

257363 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA) By similarity.
Pathway	Glycolipid biosynthesis; KDO(2)-lipid A biosynthesis; KDO(2)-lipid A from CMP-3-deoxy-D-manno-octulosonate and lipid IV(A): step 1/4. Glycolipid biosynthesis; KDO(2)-lipid A biosynthesis; KDO(2)-lipid A from CMP-3-deoxy-D-manno-octulosonate and lipid IV(A): step 2/4. Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
Subcellular location	Cell inner membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the glycosyltransferase group 1 family. Contains 1 RPE1 insert domain.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sugar binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 462	462	3-deoxy-D-manno-octulosonic-acid transferase		PRO_0000286457
Regions
	Transmembrane	2 – 22	21	Helical; Signal-anchor; Potential
	Domain	47 – 90	44	RPE1 insert

Sequences

	Sequence		Length	Mass (Da)
	Q68XV7 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: D23F37C837B00178	FASTA	462	52,900
	10 20 30 40 50 60 MMLLYYILSF ILLPVYFIII FIRLLIGKED IRRIQERFAI GKQRQNSLLD LQMSVNQEGF 70 80 90 100 110 120 KVDTEHKATS YVYIHRNASL MYKLSLERSY AQSLVWIHAA SVGEVMTSLT LIHNICKLAP 130 140 150 160 170 180 NVRFLITSWT NTSAKILSTK LPKIATHQFL PIDNVIFTRK FLSNWKPDLG IFIESELWPC 190 200 210 220 230 240 IINEGAKHCK LLLVNARISN KSFKTWLKRK KFFQLIIKNF SKIIVQSECD LQKFNALGIS 250 260 270 280 290 300 DAMNLGNIKF ANEKLLVNQE KLSKLSLHLD NRRVVVFAST HPEDEEVILP IINNLKEQFV 310 320 330 340 350 360 DCYIILIPRH PERVKSILNN CKCHNLLATA KSQNDLPVLS DDIYIVDRFG EMGLFFSVAT 370 380 390 400 410 420 ISFIGGSFKQ GGHNILEAAY FSNCIIFGPD MSKNTDIAKG ILQNNAAIQI KNGEDLLNTL 430 440 450 460 KSLLNANNAL KLKAYRENAL KFVEHNQKIL DEYLHVIKPF LP

References

[1]

"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae." McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. , Hong C., Yu X.-J., Walker D.H., Weinstock G.M. J. Bacteriol. 186:5842-5855(2004) [PubMed: 15317790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017197 Genomic DNA. Translation: AAU03535.1.
RefSeq	YP_067017.1. NC_006142.1.
3D structure databases
ProteinModelPortal	Q68XV7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2958750.
GenomeReviews	Gene locus RT0048 in contig AE017197_GR.
KEGG	rty:RT0048.
PATRIC	17909246. VBIRicTyp34752_0045.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG664037.
OMA	HPQRFDE.
ProtClustDB	PRK05749.
Enzyme and pathway databases
BioCyc	RTYP257363:RT0048-MONOMER.
Family and domain databases
InterPro	IPR007507. Glycos_transf_N. [Graphical view]
KO	K02527.
Pfam	PF04413. Glycos_transf_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KDTA_RICTY

Accession

Primary (citable) accession number: Q68XV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program