Q68XV4 (SYC_RICTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	RT0051

Organism

Rickettsia typhi (strain ATCC VR-144 / Wilmington) [Complete proteome] [HAMAP]

Taxonomic identifier

257363 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 457	457	Cysteine--tRNA ligase HAMAP MF_00041		PRO_0000159470
Regions
	Motif	31 – 41	11	"HIGH" region HAMAP MF_00041
	Motif	272 – 276	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	29	1	Zinc By similarity
	Metal binding	214	1	Zinc By similarity
	Metal binding	239	1	Zinc By similarity
	Metal binding	243	1	Zinc By similarity
	Binding site	275	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q68XV4 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 22BBA46DE77D5384	FASTA	457	53,143
	10 20 30 40 50 60 MQLHLYNTLT RTKEVFNPQD HANVKMYVCG PTVYDNPHIG NSRSVVVYDL LYRIIIKIFG 70 80 90 100 110 120 KKAVKYVRNI TDIDDKIIDR AESLGININD LTNKVTREFH INMAYLGCML PSIEPKATEH 130 140 150 160 170 180 IDVMIEIIER LIAQDHAYIT DNHVYFDVLS APNYTELSNR NLEEMFEGVR IENSKTKKNP 190 200 210 220 230 240 QDFVLWKPAK QNEPKNVNFS SPWGLGRPGW HIECSAMSYK YLGENFDIHG GGADLIFPHH 250 260 270 280 290 300 TNEIAQSRCA FPSSTYAKYW IHNGFLTVNG EKMSKSLGNF ITVRDLMDKQ IQGEVVRLFL 310 320 330 340 350 360 LSSHYRRPLD YNDKAIDDAK KTLDYWYRAI KEINVQKVDL PSDFMQSLFD DMNTPLAIKI 370 380 390 400 410 420 INDYAKGVFI SKTEAERKFN ASAIITCANF IGLMRKTQYE WFNNDIDKLY INELINKRLK 430 440 450 AKKQKNWLLA DKIRNQLLEK KIILEDKSDC TTIWRKE

References

[1]

"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae." McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. , Hong C., Yu X.-J., Walker D.H., Weinstock G.M. J. Bacteriol. 186:5842-5855(2004) [PubMed: 15317790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017197 Genomic DNA. Translation: AAU03538.1.
RefSeq	YP_067020.1. NC_006142.1.
3D structure databases
ProteinModelPortal	Q68XV4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2958761.
GenomeReviews	Gene locus RT0051 in contig AE017197_GR.
KEGG	rty:RT0051.
PATRIC	17909254. VBIRicTyp34752_0049.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG327651.
OMA	DFDALNM.
ProtClustDB	PRK00260.
Enzyme and pathway databases
BioCyc	RTYP257363:RT0051-MONOMER.
Family and domain databases
HAMAP	MF_00041. Cys_tRNA_synth. [Tree]
InterPro	IPR015803. Cys-tRNA-synt. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01883.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. CysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_RICTY

Accession

Primary (citable) accession number: Q68XV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program