Q68XV0 (SAM_RICTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
S-adenosylmethionine uptake transporter

Gene names

Name:	sam
Ordered Locus Names:	RT0056

Organism

Rickettsia typhi (strain ATCC VR-144 / Wilmington) [Complete proteome] [HAMAP]

Taxonomic identifier

257363 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transports S-adenosylmethionine. Ref.2
Enzyme regulation	Transport is inhibited by S-adenosylethionine and to a lesser extent by S-adenosylhomocysteine. Unlike eukaryotic transporters is not inhibited by sinfungin. Also inhibited by 2.4-dinitrophenol, suggesting transport is an energy-dependent process. Ref.2
Subcellular location	Cell inner membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the drug/metabolite transporter (DMT) superfamily. 10 TMS drug/metabolite exporter (DME) (TC 2.A.7.3) family. [View classification] Contains 2 DUF6 domains.

Ontologies

Keywords
Biological process	Amino-acid transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Repeat Transmembrane Transmembrane helix
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	amino acid transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	S-adenosylmethionine uptake transporter		PRO_0000280998
Regions
	Transmembrane	8 – 28	21	Helical; Potential
	Transmembrane	41 – 61	21	Helical; Potential
	Transmembrane	72 – 91	20	Helical; Potential
	Transmembrane	98 – 118	21	Helical; Potential
	Transmembrane	121 – 141	21	Helical; Potential
	Transmembrane	148 – 168	21	Helical; Potential
	Transmembrane	178 – 198	21	Helical; Potential
	Transmembrane	207 – 227	21	Helical; Potential
	Transmembrane	237 – 257	21	Helical; Potential
	Transmembrane	260 – 280	21	Helical; Potential
	Domain	23 – 141	119	DUF6 1
	Domain	160 – 280	121	DUF6 2

Sequences

	Sequence		Length	Mass (Da)
	Q68XV0 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 34778945A87EF02A	FASTA	294	33,141
	10 20 30 40 50 60 MNDALKTYLK GICWFLLSLV TSSVNDVISK YLGTRLHSFE VAFFRFLFSS IVLLPFVVYY 70 80 90 100 110 120 GKSTLKTRHP VIHVLRGLLL FFGMTSWTYG LTIAPVTTAT VISFSIPLFT LILAVFILNE 130 140 150 160 170 180 NIIWQRWVVT VVGFIGLVVM LKPHTKDFNP EILYLILAAI SFAMLDIINK KFVVKESMLS 190 200 210 220 230 240 MLFYSAIVTA IVSLPVSMEY WITPSSFELT LLFVLGSSGS FILFFLLKAF TMVDATATAP 250 260 270 280 290 YRYLELVISA IAAYFIFNEF PDKSTAHGAV IIIPATLFIM YSEKKSMSSK HESQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae." McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. , Hong C., Yu X.-J., Walker D.H., Weinstock G.M. J. Bacteriol. 186:5842-5855(2004) [PubMed: 15317790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-144 / Wilmington.
[2]	"S-adenosylmethionine transport in Rickettsia prowazekii." Tucker A.M., Winkler H.H., Driskell L.O., Wood D.O. J. Bacteriol. 185:3031-3035(2003) [PubMed: 12730161] [Abstract] Cited for: FUNCTION, ENZYME REGULATION. Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017197 Genomic DNA. Translation: AAU03542.1.
RefSeq	YP_067024.1. NC_006142.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2958728.
GenomeReviews	Gene locus RT0056 in contig AE017197_GR.
KEGG	rty:RT0056.
PATRIC	17909270. VBIRicTyp34752_0056.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG559298.
OMA	QRWVVTV.
ProtClustDB	CLSK870734.
Enzyme and pathway databases
BioCyc	RTYP257363:RT0056-MONOMER.
Family and domain databases
InterPro	IPR000620. DMT. [Graphical view]
KO	K15270.
Pfam	PF00892. EamA. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAM_RICTY

Accession

Primary (citable) accession number: Q68XV0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program