Reviewed, UniProtKB/Swiss-Prot Q68XU6 (QUEF_RICTY)

Names and origin

Protein names

Recommended name:
    NADPH-dependent 7-cyano-7-deazaguanine reductase
    EC=1.7.1.13
Alternative name(s):
    7-cyano-7-carbaguanine reductase
    PreQ(0) reductase
    NADPH-dependent nitrile oxidoreductase

Gene names

Name:	queF
Ordered Locus Names:	RT0060

Organism

Rickettsia typhi [Complete proteome] [HAMAP]

Taxonomic identifier

785 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity.
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00817
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00817
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP queuine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00817		PRO_0000163055

Sequences

	Sequence		Length	Mass (Da)
	Q68XU6-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 3B97D9947018FD9F	FASTA	272	31,096
	10 20 30 40 50 60 MPLSTSLLGK KSTYKDSYDA TLLFKIPRIN NRNVLGINNN LPFYGVDIWN TYEISCLNKN 70 80 90 100 110 120 GKPLVGIGTF YIPADSENIV ESKSFKLYLN SFNNFIVKSI EELEQIILQD LSNVTCAKVT 130 140 150 160 170 180 GRIFPINTKI EFGIPSGKNI DNLDIVCNNY GPPDNSLIEY EDVLVEEEIH SNLLKSNCLV 190 200 210 220 230 240 TGQPDWGTIV IKYKGKKLKY DSFLRYLISF RNCNEFAEQC AERIFIDIKN AINLDFLSIY 250 260 270 IVYTRRGGID ICPYRSTDKS YALPSNKRFI RQ

References

[1]

"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae." McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. , Hong C., Yu X.-J., Walker D.H., Weinstock G.M. J. Bacteriol. 186:5842-5855(2004) [PubMed: 15317790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017197 Genomic DNA. Translation: AAU03546.1.
RefSeq	YP_067028.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2958721.
GenomeReviews	Gene locus RT0060 in contig AE017197_GR.
KEGG	rty:RT0060.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q68XU6.
OMA	INPFRSN
Enzyme and pathway databases
BioCyc	RTYP257363:RT0060-MON.
BRENDA	1.7.1.13. 281221.
Family and domain databases
HAMAP	MF_00817. [Tree]
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_RICTY

Accession

Primary (citable) accession number: Q68XU6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	October 11, 2004
Last modified:	November 24, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)