Q68XU1 (PARC_RICTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA topoisomerase 4 subunit A
EC=5.99.1.3

Alternative name(s):
Topoisomerase IV subunit A

Gene names

Name:	parC
Ordered Locus Names:	RT0065

Organism

Rickettsia typhi (strain ATCC VR-144 / Wilmington) [Complete proteome] [HAMAP]

Taxonomic identifier

257363 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group › Rickettsia typhi

Protein attributes

Sequence length	737 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule By similarity. HAMAP-Rule MF_00936
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_00936
Subunit structure	Heterotetramer composed of ParC and ParE By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity HAMAP-Rule MF_00936.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family. ParC type 1 subfamily.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	DNA-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular_component	chromosome Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: InterPro DNA topoisomerase type II (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 737	737	DNA topoisomerase 4 subunit A HAMAP-Rule MF_00936		PRO_0000273115
Sites
	Active site	120	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity
	Site	40	1	Interaction with DNA By similarity
	Site	76	1	Interaction with DNA By similarity
	Site	78	1	Interaction with DNA By similarity
	Site	119	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q68XU1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: CF736359974EBB90	FASTA	737	83,414
	10 20 30 40 50 60 MKEAKIENID FGNALSERYL AYALSTIMSR SLPDVRDGLK PVHRRLLYAM LQLRLEPNSG 70 80 90 100 110 120 YKKCARVVGD VIGKYHPHGD VAVYDTLVRL AQHFSLRYPL IDGQGNFGSI DGDNAAAMRY 130 140 150 160 170 180 TESRMTEICM LLMKDIDKDT VDFRSTYDDS DLEPVIMPAS FPNLLANGSE GIAVGMATNI 190 200 210 220 230 240 PPHNLHELCD ALLYLIDNPQ AGVNDIMNFI KGPDFPTGGI IIDQTKVINA AYTTGRGSFR 250 260 270 280 290 300 VRSRWEKEEL SYGTYQIVVT EIPYQVQKSK LIEQIAILLK DKKIPLISSI RDESTDIIRL 310 320 330 340 350 360 VIEPRDRSCD PQIVMESLFK LTNLESRIQL NMNVIGSNNV PRVMNIVEIL QEFLVHRQNI 370 380 390 400 410 420 ITRRSTYLLN KIKQRLEILK VLKIVYLNLD EIIEIIRRED EPKTIIMERF KISEIQVEVI 430 440 450 460 470 480 LNTRLRALQK LEEHAIIDEH SNLQKQQAIL EKILKNHKEL WKIVKKEIKS VQTQFGLNTI 490 500 510 520 530 540 IGARRTSFEE VDLTNQVIDI TAFITKEPIT IICSKMGWIR LLKGHNTDLS TIKYKEGDAE 550 560 570 580 590 600 KFIIEAYTTD KILIISSKGR FFTLLADNIS KGKGTAVSIK LLVDIGNNDI TNILVYKPYQ 610 620 630 640 650 660 LLLLASSIGK GFLVNSNEVI AQTKAGKQIM NIPEGYACIT CLPVNGNSIA CINESRRLLI 670 680 690 700 710 720 FNINEIPEMK KGQGVVLQRF KNAKLLDIKI FNKEDGLSWN DGKKIQLEKN IVAFLGKRGS 730 AGTLPPIGFP KNNRFSS

References

[1]

"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae." McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. , Hong C., Yu X.-J., Walker D.H., Weinstock G.M. J. Bacteriol. 186:5842-5855(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017197 Genomic DNA. Translation: AAU03551.1.
RefSeq	YP_067033.1. NC_006142.1.
3D structure databases
ProteinModelPortal	Q68XU1.
ModBase	Search...
Protein-protein interaction databases
STRING	257363.RT0065.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAU03551; AAU03551; RT0065.
GeneID	2958686.
KEGG	rty:RT0065.
PATRIC	17909288. VBIRicTyp34752_0065.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0188.
HOGENOM	HOG000076277.
KO	K02621.
OMA	KYKDGGL.
ProtClustDB	PRK05561.
Enzyme and pathway databases
BioCyc	RTYP257363:GJEQ-68-MONOMER.
Family and domain databases
Gene3D	3.30.1360.40. 1 hit. 3.90.199.10. 2 hits.
HAMAP	MF_00936. ParC_type1.
InterPro	IPR024946. Arg_repress_C-like. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013760. Topo_IIA_like_dom. IPR005742. TopoIV_A_Gneg. [Graphical view]
Pfam	PF03989. DNA_gyraseA_C. 2 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01062. parC_Gneg. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PARC_RICTY

Accession

Primary (citable) accession number: Q68XU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program