Reviewed, UniProtKB/Swiss-Prot Q68XR8 (TILS_RICTY)

Names and origin

Protein names

Recommended name:
    tRNA(Ile)-lysidine synthase
    EC=6.3.4.-
Alternative name(s):
    tRNA(Ile)-lysidine synthetase
    tRNA(Ile)-2-lysyl-cytidine synthase

Gene names

Name:	tilS
Ordered Locus Names:	RT0088

Organism

Rickettsia typhi [Complete proteome] [HAMAP]

Taxonomic identifier

785 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine By similarity.
Subcellular location	Cytoplasm Probable.
Domain	The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding. HAMAP MF_01161
Sequence similarities	Belongs to the tRNA(Ile)-lysidine synthase family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ligase activity, forming carbon-nitrogen bonds Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 430	430	tRNA(Ile)-lysidine synthase HAMAP MF_01161		PRO_0000181758
Regions
	Nucleotide binding	27 – 32	6	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q68XR8-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: DCE2ADDEE3001E73	FASTA	430	50,397
	10 20 30 40 50 60 MLYEKFEYNI NNLVGNFGLS KIAIAVSGGS DSVALLYLAN IWAEKNNIEL FIISVDHNLR 70 80 90 100 110 120 AQSKQENYYI QSISNNLKRK YYNLSFDHQN NFSNLQARAR EGRYDLMTNL CLELDVLVLL 130 140 150 160 170 180 TAHHEDDYVE NFCLRLERNS GIFGLSSSNI HWYNNIQIIR PLYNIPKSEL VKYLVTNKIK 190 200 210 220 230 240 WFEDESNLSD KYRRNIIRQK LFKEENYIKA EISVQQLKTN KLIEDELKPE LISAIGEAVK 250 260 270 280 290 300 IFEYGFTFLD LVKFDKFSNE VKVQIINFLL IMISGQSRSA RFYSIAPILK LISQNVNFKK 310 320 330 340 350 360 TVHGCVITRI QNELLIYREF GKKLPKSKIL LDKSVIWDNR FCITKNQETP DCVITYLSLE 370 380 390 400 410 420 DYKVIKKQLD LKHLKNLSCK NHNAILFTLP IIKILEKVIA IPHISYYDND MWNFEVSFAP 430 NFVSRFTHFC

References

[1]

"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae." McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. , Hong C., Yu X.-J., Walker D.H., Weinstock G.M. J. Bacteriol. 186:5842-5855(2004) [PubMed: 15317790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017197 Genomic DNA. Translation: AAU03574.1.
RefSeq	YP_067056.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2959100.
GenomeReviews	Gene locus RT0088 in contig AE017197_GR.
KEGG	rty:RT0088.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG586536.
OMA	NNIQIIR.
Enzyme and pathway databases
BioCyc	RTYP257363:RT0088-MON.
Family and domain databases
HAMAP	MF_01161. [Tree]
InterPro	IPR012094. Lysidine-tRNA-synth. IPR012795. Lysidine-tRNA-synth_N. IPR011063. PP_loop. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11807:SF2. ATPase_MesJ_YaeO. 1 hit.
Pfam	PF01171. ATP_bind_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TILS_RICTY

Accession

Primary (citable) accession number: Q68XR8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	October 11, 2004
Last modified:	December 15, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)