Reviewed, UniProtKB/Swiss-Prot Q68XN9 (DHSA_RICTY)

Names and origin

Protein names

Recommended name:
    Succinate dehydrogenase flavoprotein subunit
    EC=1.3.99.1

Gene names

Name:	sdhA
Ordered Locus Names:	RT0117

Organism

Rickettsia typhi [Complete proteome] [HAMAP]

Taxonomic identifier

785 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	596 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Succinate + acceptor = fumarate + reduced acceptor.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (bacterial route): step 1/1.
Subunit structure	Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, cytochrome b-556, and an hydrophobic anchor protein.
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro succinate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 596	596	Succinate dehydrogenase flavoprotein subunit		PRO_0000280978
Regions
	Nucleotide binding	41 – 56	16	FAD By similarity
Sites
	Active site	290	1	Proton acceptor By similarity
	Binding site	246	1	Substrate By similarity
	Binding site	258	1	Substrate By similarity
	Binding site	357	1	Substrate By similarity
	Binding site	402	1	Substrate By similarity
Amino acid modifications
	Modified residue	49	1	Tele-8alpha-FAD histidine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q68XN9-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 5B4F534F1490ECE1	FASTA	596	65,743
	10 20 30 40 50 60 MTKAYNIIHH KFDVVVVGAG GAGLRSAFGM AKEGLNTACI TKIFPTRSHT VAAQGGISAA 70 80 90 100 110 120 LGNMGEDDWR WHMYDTVKGS DWLGDQDAIE YMCKNAPDAI LELEHYGVPF SRTEDGKIYQ 130 140 150 160 170 180 RPFGGMTTEY GKGKAAQRTC AAADRTGHAI LHTLYQQSLK HKVQFFIEYF AIDLLMEDGE 190 200 210 220 230 240 CRGVVAWNLD DGSLHCFRAH NVVLATGGYG RAYFSATSAH TCTGDGGGMV IRAGLPLQDM 250 260 270 280 290 300 EFVQFHPTGI YSAGCLITEG ARGEGGYLVN ANGERFMERY APAAKDLASR DVVSRAMTIE 310 320 330 340 350 360 IREGRGVGEY KDHVFLHLNH LSPEILHRRL PGISETAKIF AGVDVTKDPI PVLPTVHYNM 370 380 390 400 410 420 GGIPTNYHGQ VIIKDGKNHN SIVNGIMAIG EAACVSVHGA NRLGSNSLLD LVVFGRSSAL 430 440 450 460 470 480 KAAELIKPVS PHKPLKKEIL EKIINRFDKI RHANGNVLVA DLRLQMQRTM QSHVSVFRTQ 490 500 510 520 530 540 ELLDEGARMI SEIRNRYKDI KINDKSLIWN SDLVEALELD NLLDQALVTV YSAAARKESR 550 560 570 580 590 GAHAREDYPD RNDIDWIKHT LSSIDDSGQI ILDYKPVTLT TLTDEISTIP PVKRIY

References

[1]

"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae." McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. , Hong C., Yu X.-J., Walker D.H., Weinstock G.M. J. Bacteriol. 186:5842-5855(2004) [PubMed: 15317790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017197 Genomic DNA. Translation: AAU03603.1.
RefSeq	YP_067085.1.
3D structure databases
HSSP	HSSP built from PDB template 2ACZ based on UniProtKB P0AC41.
SMR	Q68XN9. Positions 5-596.
ModBase	Search...
Genome annotation databases
GeneID	2959329.
GenomeReviews	Gene locus RT0117 in contig AE017197_GR.
KEGG	rty:RT0117.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG293998.
OMA	VYELEHW.
Enzyme and pathway databases
BioCyc	RTYP257363:RT0117-MONOMER.
BRENDA	1.3.99.1. 281221.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR003952. FRD_SDH_FAD_BS. IPR015939. Fum_Rdtase/Succ_DH_flav-like_C. IPR004112. Fum_Rdtase/Succ_DH_flav_C. IPR011281. Succ_DH_flav_su_fwd. IPR014006. Succ_Dhase_frdA_Gneg. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
TIGRFAMs	TIGR01816. sdhA_forward. 1 hit. TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITE	PS00504. FRD_SDH_FAD_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHSA_RICTY

Accession

Primary (citable) accession number: Q68XN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	October 11, 2004
Last modified:	February 9, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)