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Q68XI3

- DNAJ_RICTY

UniProt

Q68XI3 - DNAJ_RICTY

Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Rickettsia typhi (strain ATCC VR-144 / Wilmington)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotation

    Cofactori

    Binds 2 zinc ions per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi146 – 1461Zinc 1UniRule annotation
    Metal bindingi149 – 1491Zinc 1UniRule annotation
    Metal bindingi163 – 1631Zinc 2UniRule annotation
    Metal bindingi166 – 1661Zinc 2UniRule annotation
    Metal bindingi185 – 1851Zinc 2UniRule annotation
    Metal bindingi188 – 1881Zinc 2UniRule annotation
    Metal bindingi199 – 1991Zinc 1UniRule annotation
    Metal bindingi202 – 2021Zinc 1UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri133 – 21179CR-typeUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: InterPro
    2. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW
    2. protein folding Source: InterPro
    3. response to heat Source: InterPro

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    DNA replication, Stress response

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciRTYP257363:GJEQ-184-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chaperone protein DnaJUniRule annotation
    Gene namesi
    Name:dnaJUniRule annotation
    Ordered Locus Names:RT0175
    OrganismiRickettsia typhi (strain ATCC VR-144 / Wilmington)
    Taxonomic identifieri257363 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
    ProteomesiUP000000604: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 370370Chaperone protein DnaJPRO_0000070874Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi257363.RT0175.

    Structurei

    3D structure databases

    ProteinModelPortaliQ68XI3.
    SMRiQ68XI3. Positions 3-73.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 6865JUniRule annotationAdd
    BLAST
    Repeati146 – 1538CXXCXGXG motif
    Repeati163 – 1708CXXCXGXG motif
    Repeati185 – 1928CXXCXGXG motif
    Repeati199 – 2068CXXCXGXG motif

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi75 – 11642Gly-richAdd
    BLAST

    Domaini

    The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation

    Sequence similaritiesi

    Belongs to the DnaJ family.UniRule annotation
    Contains 1 CR-type zinc finger.UniRule annotation
    Contains 1 J domain.UniRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri133 – 21179CR-typeUniRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0484.
    HOGENOMiHOG000226717.
    KOiK03686.
    OMAiDLFGMAG.
    OrthoDBiEOG6BPDKP.

    Family and domain databases

    Gene3Di1.10.287.110. 1 hit.
    2.10.230.10. 1 hit.
    HAMAPiMF_01152. DnaJ.
    InterProiIPR012724. DnaJ.
    IPR002939. DnaJ_C.
    IPR001623. DnaJ_domain.
    IPR018253. DnaJ_domain_CS.
    IPR008971. HSP40/DnaJ_pept-bd.
    IPR001305. HSP_DnaJ_Cys-rich_dom.
    [Graphical view]
    PfamiPF01556. CTDII. 1 hit.
    PF00226. DnaJ. 1 hit.
    PF00684. DnaJ_CXXCXGXG. 1 hit.
    [Graphical view]
    PRINTSiPR00625. JDOMAIN.
    SMARTiSM00271. DnaJ. 1 hit.
    [Graphical view]
    SUPFAMiSSF46565. SSF46565. 1 hit.
    SSF49493. SSF49493. 3 hits.
    SSF57938. SSF57938. 1 hit.
    TIGRFAMsiTIGR02349. DnaJ_bact. 1 hit.
    PROSITEiPS00636. DNAJ_1. 1 hit.
    PS50076. DNAJ_2. 1 hit.
    PS51188. ZF_CR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q68XI3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQDYYQVLG VSKTASQADI KKAYLKLAKQ YHPDTTNAHD AEKKFKEINA    50
    AYDVLKDEQK RAAYDRFGHD AFQNQQARGG GNNSGFHHDI NDIFGDFFSD 100
    FMGSGRRKQT SSKIRGSDLK YDLTIKLEEA FHGIEKNISF SSEVKCDACH 150
    GTGSEKGETV TTCDSCGGVG VTRIQQGFFT LEQTCHKCQG NGQIIKNPCK 200
    KCHGMGRYHK QRNLSINIPA GVENGTRIRH SGEGEAGIRG GNNGDLYVDI 250
    AIKPHDIYKI DGANLHCKLP ISFVHAALGG EIEVPVIEGG KVKLTIPAGT 300
    QNGDQLRLRS KGMSKIRSTI RGDMLTHIHV EVPKNLSKRQ RELLEEFKKE 350
    SINEKENDSS FFNKMKSMWS 370
    Length:370
    Mass (Da):41,145
    Last modified:October 11, 2004 - v1
    Checksum:i15265F965AA5DD09
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017197 Genomic DNA. Translation: AAU03659.1.
    RefSeqiYP_067141.1. NC_006142.1.

    Genome annotation databases

    EnsemblBacteriaiAAU03659; AAU03659; RT0175.
    GeneIDi2958519.
    KEGGirty:RT0175.
    PATRICi17909526. VBIRicTyp34752_0182.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017197 Genomic DNA. Translation: AAU03659.1 .
    RefSeqi YP_067141.1. NC_006142.1.

    3D structure databases

    ProteinModelPortali Q68XI3.
    SMRi Q68XI3. Positions 3-73.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 257363.RT0175.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAU03659 ; AAU03659 ; RT0175 .
    GeneIDi 2958519.
    KEGGi rty:RT0175.
    PATRICi 17909526. VBIRicTyp34752_0182.

    Phylogenomic databases

    eggNOGi COG0484.
    HOGENOMi HOG000226717.
    KOi K03686.
    OMAi DLFGMAG.
    OrthoDBi EOG6BPDKP.

    Enzyme and pathway databases

    BioCyci RTYP257363:GJEQ-184-MONOMER.

    Family and domain databases

    Gene3Di 1.10.287.110. 1 hit.
    2.10.230.10. 1 hit.
    HAMAPi MF_01152. DnaJ.
    InterProi IPR012724. DnaJ.
    IPR002939. DnaJ_C.
    IPR001623. DnaJ_domain.
    IPR018253. DnaJ_domain_CS.
    IPR008971. HSP40/DnaJ_pept-bd.
    IPR001305. HSP_DnaJ_Cys-rich_dom.
    [Graphical view ]
    Pfami PF01556. CTDII. 1 hit.
    PF00226. DnaJ. 1 hit.
    PF00684. DnaJ_CXXCXGXG. 1 hit.
    [Graphical view ]
    PRINTSi PR00625. JDOMAIN.
    SMARTi SM00271. DnaJ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46565. SSF46565. 1 hit.
    SSF49493. SSF49493. 3 hits.
    SSF57938. SSF57938. 1 hit.
    TIGRFAMsi TIGR02349. DnaJ_bact. 1 hit.
    PROSITEi PS00636. DNAJ_1. 1 hit.
    PS50076. DNAJ_2. 1 hit.
    PS51188. ZF_CR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC VR-144 / Wilmington.

    Entry informationi

    Entry nameiDNAJ_RICTY
    AccessioniPrimary (citable) accession number: Q68XI3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Rickettsia typhi
      Rickettsia typhi (strain Wilmington): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3