Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q68XI3 (DNAJ_RICTY) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chaperone protein DnaJ
Gene names
Name:dnaJ
Ordered Locus Names:RT0175
OrganismRickettsia typhi (strain ATCC VR-144 / Wilmington) [Complete proteome] [HAMAP]
Taxonomic identifier257363 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group

Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins By similarity. HAMAP-Rule MF_01152

Cofactor

Binds 2 zinc ions per monomer By similarity. HAMAP-Rule MF_01152

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01152

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01152.

Domain

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity By similarity. HAMAP-Rule MF_01152

Sequence similarities

Belongs to the DnaJ family.

Contains 1 CR-type zinc finger.

Contains 1 J domain.

Ontologies

Keywords
   Biological processDNA replication
Stress response
   Cellular componentCytoplasm
   DomainRepeat
Zinc-finger
   LigandMetal-binding
Zinc
   Molecular functionChaperone
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

protein folding

Inferred from electronic annotation. Source: InterPro

response to heat

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 370370Chaperone protein DnaJ HAMAP-Rule MF_01152
PRO_0000070874

Regions

Domain4 – 6865J
Repeat146 – 1538CXXCXGXG motif HAMAP-Rule MF_01152
Repeat163 – 1708CXXCXGXG motif HAMAP-Rule MF_01152
Repeat185 – 1928CXXCXGXG motif HAMAP-Rule MF_01152
Repeat199 – 2068CXXCXGXG motif HAMAP-Rule MF_01152
Zinc finger133 – 21179CR-type HAMAP-Rule MF_01152
Compositional bias75 – 11642Gly-rich HAMAP-Rule MF_01152

Sites

Metal binding1461Zinc 1 By similarity
Metal binding1491Zinc 1 By similarity
Metal binding1631Zinc 2 By similarity
Metal binding1661Zinc 2 By similarity
Metal binding1851Zinc 2 By similarity
Metal binding1881Zinc 2 By similarity
Metal binding1991Zinc 1 By similarity
Metal binding2021Zinc 1 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q68XI3 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 15265F965AA5DD09

FASTA37041,145
        10         20         30         40         50         60 
MSQDYYQVLG VSKTASQADI KKAYLKLAKQ YHPDTTNAHD AEKKFKEINA AYDVLKDEQK 

        70         80         90        100        110        120 
RAAYDRFGHD AFQNQQARGG GNNSGFHHDI NDIFGDFFSD FMGSGRRKQT SSKIRGSDLK 

       130        140        150        160        170        180 
YDLTIKLEEA FHGIEKNISF SSEVKCDACH GTGSEKGETV TTCDSCGGVG VTRIQQGFFT 

       190        200        210        220        230        240 
LEQTCHKCQG NGQIIKNPCK KCHGMGRYHK QRNLSINIPA GVENGTRIRH SGEGEAGIRG 

       250        260        270        280        290        300 
GNNGDLYVDI AIKPHDIYKI DGANLHCKLP ISFVHAALGG EIEVPVIEGG KVKLTIPAGT 

       310        320        330        340        350        360 
QNGDQLRLRS KGMSKIRSTI RGDMLTHIHV EVPKNLSKRQ RELLEEFKKE SINEKENDSS 

       370 
FFNKMKSMWS 

« Hide

References

[1]"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae."
McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. expand/collapse author list , Hong C., Yu X.-J., Walker D.H., Weinstock G.M.
J. Bacteriol. 186:5842-5855(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017197 Genomic DNA. Translation: AAU03659.1.
RefSeqYP_067141.1. NC_006142.1.

3D structure databases

ProteinModelPortalQ68XI3.
SMRQ68XI3. Positions 3-73.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING257363.RT0175.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU03659; AAU03659; RT0175.
GeneID2958519.
KEGGrty:RT0175.
PATRIC17909526. VBIRicTyp34752_0182.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0484.
HOGENOMHOG000226717.
KOK03686.
OMADLFGMAG.
OrthoDBEOG6BPDKP.

Enzyme and pathway databases

BioCycRTYP257363:GJEQ-184-MONOMER.

Family and domain databases

Gene3D1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPMF_01152. DnaJ.
InterProIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamPF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSPR00625. JDOMAIN.
SMARTSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsTIGR02349. DnaJ_bact. 1 hit.
PROSITEPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNAJ_RICTY
AccessionPrimary (citable) accession number: Q68XI3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: October 11, 2004
Last modified: May 14, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia typhi

Rickettsia typhi (strain Wilmington): entries and gene names