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Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Rickettsia typhi (strain ATCC VR-144 / Wilmington)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi146 – 1461Zinc 1UniRule annotation
Metal bindingi149 – 1491Zinc 1UniRule annotation
Metal bindingi163 – 1631Zinc 2UniRule annotation
Metal bindingi166 – 1661Zinc 2UniRule annotation
Metal bindingi185 – 1851Zinc 2UniRule annotation
Metal bindingi188 – 1881Zinc 2UniRule annotation
Metal bindingi199 – 1991Zinc 1UniRule annotation
Metal bindingi202 – 2021Zinc 1UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri133 – 21179CR-typeUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: InterPro
  2. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
  2. protein folding Source: InterPro
  3. response to heat Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

DNA replication, Stress response

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciRTYP257363:GJEQ-184-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJUniRule annotation
Gene namesi
Name:dnaJUniRule annotation
Ordered Locus Names:RT0175
OrganismiRickettsia typhi (strain ATCC VR-144 / Wilmington)
Taxonomic identifieri257363 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
ProteomesiUP000000604 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 370370Chaperone protein DnaJPRO_0000070874Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi257363.RT0175.

Structurei

3D structure databases

ProteinModelPortaliQ68XI3.
SMRiQ68XI3. Positions 3-73.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 6865JUniRule annotationAdd
BLAST
Repeati146 – 1538CXXCXGXG motif
Repeati163 – 1708CXXCXGXG motif
Repeati185 – 1928CXXCXGXG motif
Repeati199 – 2068CXXCXGXG motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi75 – 11642Gly-richAdd
BLAST

Domaini

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation

Sequence similaritiesi

Belongs to the DnaJ family.UniRule annotation
Contains 1 CR-type zinc finger.UniRule annotation
Contains 1 J domain.UniRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri133 – 21179CR-typeUniRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0484.
HOGENOMiHOG000226717.
KOiK03686.
OMAiPHLETCD.
OrthoDBiEOG6BPDKP.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPiMF_01152. DnaJ.
InterProiIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamiPF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsiTIGR02349. DnaJ_bact. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q68XI3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQDYYQVLG VSKTASQADI KKAYLKLAKQ YHPDTTNAHD AEKKFKEINA
60 70 80 90 100
AYDVLKDEQK RAAYDRFGHD AFQNQQARGG GNNSGFHHDI NDIFGDFFSD
110 120 130 140 150
FMGSGRRKQT SSKIRGSDLK YDLTIKLEEA FHGIEKNISF SSEVKCDACH
160 170 180 190 200
GTGSEKGETV TTCDSCGGVG VTRIQQGFFT LEQTCHKCQG NGQIIKNPCK
210 220 230 240 250
KCHGMGRYHK QRNLSINIPA GVENGTRIRH SGEGEAGIRG GNNGDLYVDI
260 270 280 290 300
AIKPHDIYKI DGANLHCKLP ISFVHAALGG EIEVPVIEGG KVKLTIPAGT
310 320 330 340 350
QNGDQLRLRS KGMSKIRSTI RGDMLTHIHV EVPKNLSKRQ RELLEEFKKE
360 370
SINEKENDSS FFNKMKSMWS
Length:370
Mass (Da):41,145
Last modified:October 11, 2004 - v1
Checksum:i15265F965AA5DD09
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017197 Genomic DNA. Translation: AAU03659.1.
RefSeqiYP_067141.1. NC_006142.1.

Genome annotation databases

EnsemblBacteriaiAAU03659; AAU03659; RT0175.
GeneIDi2958519.
KEGGirty:RT0175.
PATRICi17909526. VBIRicTyp34752_0182.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017197 Genomic DNA. Translation: AAU03659.1.
RefSeqiYP_067141.1. NC_006142.1.

3D structure databases

ProteinModelPortaliQ68XI3.
SMRiQ68XI3. Positions 3-73.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi257363.RT0175.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU03659; AAU03659; RT0175.
GeneIDi2958519.
KEGGirty:RT0175.
PATRICi17909526. VBIRicTyp34752_0182.

Phylogenomic databases

eggNOGiCOG0484.
HOGENOMiHOG000226717.
KOiK03686.
OMAiPHLETCD.
OrthoDBiEOG6BPDKP.

Enzyme and pathway databases

BioCyciRTYP257363:GJEQ-184-MONOMER.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPiMF_01152. DnaJ.
InterProiIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamiPF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsiTIGR02349. DnaJ_bact. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-144 / Wilmington.

Entry informationi

Entry nameiDNAJ_RICTY
AccessioniPrimary (citable) accession number: Q68XI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: October 11, 2004
Last modified: January 7, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia typhi
    Rickettsia typhi (strain Wilmington): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.