Q68XG6 (RNH2_RICTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease HII
Short name=RNase HII
EC=3.1.26.4

Gene names

Name:	rnhB
Ordered Locus Names:	RT0192

Organism

Rickettsia typhi (strain ATCC VR-144 / Wilmington) [Complete proteome] [HAMAP]

Taxonomic identifier

257363 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group › Rickettsia typhi

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity. HAMAP-Rule MF_00052
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00052
Cofactor	Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00052.
Sequence similarities	Belongs to the RNase HII family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA catabolic process Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro manganese ion binding Inferred from electronic annotation. Source: HAMAP ribonuclease H activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 193	193	Ribonuclease HII HAMAP-Rule MF_00052		PRO_0000111614
Sites
	Metal binding	21	1	Divalent metal cation By similarity
	Metal binding	22	1	Divalent metal cation By similarity
	Metal binding	112	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q68XG6 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 9C0902A70186E618	FASTA	193	21,680
	10 20 30 40 50 60 MEVNLLQYEQ KYHNYIVAGV DEAGRGSLVG PVVASAVIID KVDIISGIKD SKKLSKNKRD 70 80 90 100 110 120 ILYEQITSNY VWSTAIIEHT EIDDINILEA TKKACTIAVA NLSLKPEKIL VDGNMKFSDV 130 140 150 160 170 180 RFISIINGDN LSLSIAAASI IAKVTRDRLM LELSAEYPQY LWHKNYGYGT REHIEAINTH 190 GLSSYHRKSF KFC

References

[1]

"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae." McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. , Hong C., Yu X.-J., Walker D.H., Weinstock G.M. J. Bacteriol. 186:5842-5855(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017197 Genomic DNA. Translation: AAU03676.1.
RefSeq	YP_067158.1. NC_006142.1.
3D structure databases
ProteinModelPortal	Q68XG6.
ModBase	Search...
Protein-protein interaction databases
STRING	257363.RT0192.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAU03676; AAU03676; RT0192.
GeneID	2958802.
KEGG	rty:RT0192.
PATRIC	17909568. VBIRicTyp34752_0203.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0164.
HOGENOM	HOG000100288.
KO	K03470.
OMA	GCSLANE.
ProtClustDB	PRK00015.
Enzyme and pathway databases
BioCyc	RTYP257363:GJEQ-202-MONOMER.
Family and domain databases
HAMAP	MF_00052_B. RNase_HII_B.
InterPro	IPR022898. RNase_HII. IPR001352. RNase_HII/HIII. IPR024567. RNase_HII/HIII_dom. IPR012337. RNaseH-like_dom. [Graphical view]
PANTHER	PTHR10954. PTHR10954. 1 hit.
Pfam	PF01351. RNase_HII. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RNH2_RICTY

Accession

Primary (citable) accession number: Q68XG6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program