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Q68X95 (LEPA_RICTY) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Elongation factor 4

Short name=EF-4
EC=3.6.5.n1
Alternative name(s):
Ribosomal back-translocase LepA
Gene names
Name:lepA
Ordered Locus Names:RT0266
OrganismRickettsia typhi (strain ATCC VR-144 / Wilmington) [Complete proteome] [HAMAP]
Taxonomic identifier257363 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group

Protein attributes

Sequence length600 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner By similarity. HAMAP-Rule MF_00071

Catalytic activity

GTP + H2O = GDP + phosphate. HAMAP-Rule MF_00071

Subcellular location

Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP-Rule MF_00071.

Sequence similarities

Belongs to the GTP-binding elongation factor family. LepA subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCell inner membrane
Cell membrane
Membrane
   LigandGTP-binding
Nucleotide-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processpositive regulation of translation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

GTPase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

ribosome binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

translation elongation factor activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 600600Elongation factor 4 HAMAP-Rule MF_00071
PRO_0000176335

Regions

Nucleotide binding17 – 226GTP By similarity
Nucleotide binding134 – 1374GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q68X95 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: B512FBCEA0D96FAD

FASTA60067,101
        10         20         30         40         50         60 
MNNQKYIRNF SIIAHIDHGK STLADRLIEH CGGLNAREMS QQVLDSMDIE KERGITIKAQ 

        70         80         90        100        110        120 
TVRLVYKAKN GNTYYLNLMD TPGHVDFSYE VSRSLAACEG SLLVVDSTQG VEAQTLANVY 

       130        140        150        160        170        180 
QAIANDHEIV PVLNKIDLAA SEPEHVKKQI EDIIGIDASE AVLISAKNGI GIDSVLEAII 

       190        200        210        220        230        240 
NKLPSPKESS TDTLKALLVD SWYDPYLGVV ILVRIIDGTL RKNMRVKMIG TNSVYTVEHV 

       250        260        270        280        290        300 
GFFTPKKHIS DVLYAGEIGF FTASIKRVSD CKVGDTITDE KKSCEQALPG FKPNIPVVFC 

       310        320        330        340        350        360 
GFYPTDSAEF EHLKDSLAKL RLNDASFEYE MESSSALGVG FRCGFLGLLH LEIIQERLSR 

       370        380        390        400        410        420 
EFNLDLITTA PSVIYKIYML DGESLEIHNP ADLPDLQKIA SMEEPWIKAT IMVPDEFIGT 

       430        440        450        460        470        480 
VLSLCKEKRG IQLDHSYISN RAKIVYKLPL NEIVYDFYDR LKSCSKGYAS FEWQMDVYEL 

       490        500        510        520        530        540 
SDLVNLRILV NGEVVDALST IVHRSRAEQR GRALCVRLKD LIPRQQIDIA IQASVGNRII 

       550        560        570        580        590        600 
ARETIKALRK DVLSKCYGGD ISRKRKLLEK QKAGKKKMRQ YGNIEIPQSA FIAALQIGGE 

« Hide

References

[1]"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae."
McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. expand/collapse author list , Hong C., Yu X.-J., Walker D.H., Weinstock G.M.
J. Bacteriol. 186:5842-5855(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017197 Genomic DNA. Translation: AAU03747.1.
RefSeqYP_067229.1. NC_006142.1.

3D structure databases

ProteinModelPortalQ68X95.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING257363.RT0266.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU03747; AAU03747; RT0266.
GeneID2958556.
KEGGrty:RT0266.
PATRIC17909718. VBIRicTyp34752_0275.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0481.
HOGENOMHOG000020624.
KOK03596.
OMAKPMVFCG.
OrthoDBEOG6ZKXQ4.
ProtClustDBPRK05433.

Enzyme and pathway databases

BioCycRTYP257363:GJEQ-279-MONOMER.

Family and domain databases

Gene3D3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_00071. LepA.
InterProIPR006297. EF-4.
IPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamPF00679. EFG_C. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSPR00315. ELONGATNFCT.
SMARTSM00838. EFG_C. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLEPA_RICTY
AccessionPrimary (citable) accession number: Q68X95
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: October 11, 2004
Last modified: April 16, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia typhi

Rickettsia typhi (strain Wilmington): entries and gene names