Q68X95 (LEPA_RICTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor 4
Short name=EF-4
EC=3.6.5.n1

Alternative name(s):
Ribosomal back-translocase LepA

Gene names

Name:	lepA
Ordered Locus Names:	RT0266

Organism

Rickettsia typhi (strain ATCC VR-144 / Wilmington) [Complete proteome] [HAMAP]

Taxonomic identifier

257363 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group › Rickettsia typhi

Protein attributes

Sequence length	600 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner By similarity. HAMAP-Rule MF_00071
Catalytic activity	GTP + H2O = GDP + phosphate. HAMAP-Rule MF_00071
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP-Rule MF_00071.
Sequence similarities	Belongs to the GTP-binding elongation factor family. LepA subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	GTP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC positive regulation of translation Inferred from electronic annotation. Source: HAMAP translational elongation Inferred from electronic annotation. Source: GOC
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: HAMAP ribosome binding Inferred from electronic annotation. Source: HAMAP translation elongation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 600	600	Elongation factor 4 HAMAP-Rule MF_00071		PRO_0000176335
Regions
	Nucleotide binding	17 – 22	6	GTP By similarity
	Nucleotide binding	134 – 137	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q68X95 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: B512FBCEA0D96FAD	FASTA	600	67,101
	10 20 30 40 50 60 MNNQKYIRNF SIIAHIDHGK STLADRLIEH CGGLNAREMS QQVLDSMDIE KERGITIKAQ 70 80 90 100 110 120 TVRLVYKAKN GNTYYLNLMD TPGHVDFSYE VSRSLAACEG SLLVVDSTQG VEAQTLANVY 130 140 150 160 170 180 QAIANDHEIV PVLNKIDLAA SEPEHVKKQI EDIIGIDASE AVLISAKNGI GIDSVLEAII 190 200 210 220 230 240 NKLPSPKESS TDTLKALLVD SWYDPYLGVV ILVRIIDGTL RKNMRVKMIG TNSVYTVEHV 250 260 270 280 290 300 GFFTPKKHIS DVLYAGEIGF FTASIKRVSD CKVGDTITDE KKSCEQALPG FKPNIPVVFC 310 320 330 340 350 360 GFYPTDSAEF EHLKDSLAKL RLNDASFEYE MESSSALGVG FRCGFLGLLH LEIIQERLSR 370 380 390 400 410 420 EFNLDLITTA PSVIYKIYML DGESLEIHNP ADLPDLQKIA SMEEPWIKAT IMVPDEFIGT 430 440 450 460 470 480 VLSLCKEKRG IQLDHSYISN RAKIVYKLPL NEIVYDFYDR LKSCSKGYAS FEWQMDVYEL 490 500 510 520 530 540 SDLVNLRILV NGEVVDALST IVHRSRAEQR GRALCVRLKD LIPRQQIDIA IQASVGNRII 550 560 570 580 590 600 ARETIKALRK DVLSKCYGGD ISRKRKLLEK QKAGKKKMRQ YGNIEIPQSA FIAALQIGGE

References

[1]

"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae." McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. , Hong C., Yu X.-J., Walker D.H., Weinstock G.M. J. Bacteriol. 186:5842-5855(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017197 Genomic DNA. Translation: AAU03747.1.
RefSeq	YP_067229.1. NC_006142.1.
3D structure databases
ProteinModelPortal	Q68X95.
ModBase	Search...
Protein-protein interaction databases
STRING	257363.RT0266.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAU03747; AAU03747; RT0266.
GeneID	2958556.
KEGG	rty:RT0266.
PATRIC	17909718. VBIRicTyp34752_0275.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0481.
HOGENOM	HOG000020624.
KO	K03596.
OMA	KCYGGDA.
ProtClustDB	PRK05433.
Enzyme and pathway databases
BioCyc	RTYP257363:GJEQ-279-MONOMER.
Family and domain databases
Gene3D	3.30.70.240. 1 hit.
HAMAP	MF_00071. LepA.
InterPro	IPR006297. EF-4. IPR000795. EF_GTP-bd_dom. IPR009022. EFG_III-V. IPR000640. EFG_V. IPR013842. LepA_GTP-bd_C. IPR005225. Small_GTP-bd_dom. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
PANTHER	PTHR23115:SF40. PTHR23115:SF40. 1 hit.
Pfam	PF00679. EFG_C. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF06421. LepA_C. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SMART	SM00838. EFG_C. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR01393. lepA. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEPA_RICTY

Accession

Primary (citable) accession number: Q68X95

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program