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Protein

Elongation factor 4

Gene

lepA

Organism
Rickettsia typhi (strain ATCC VR-144 / Wilmington)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.UniRule annotation

Catalytic activityi

GTP + H2O = GDP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 226GTPUniRule annotation
Nucleotide bindingi134 – 1374GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRTYP257363:GJEQ-279-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 4UniRule annotation (EC:3.6.5.n1UniRule annotation)
Short name:
EF-4UniRule annotation
Alternative name(s):
Ribosomal back-translocase LepAUniRule annotation
Gene namesi
Name:lepAUniRule annotation
Ordered Locus Names:RT0266
OrganismiRickettsia typhi (strain ATCC VR-144 / Wilmington)
Taxonomic identifieri257363 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
ProteomesiUP000000604 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 600600Elongation factor 4PRO_0000176335Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi257363.RT0266.

Structurei

3D structure databases

ProteinModelPortaliQ68X95.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 187183tr-type GAdd
BLAST

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0481.
HOGENOMiHOG000020624.
KOiK03596.
OMAiFAGIYPV.
OrthoDBiEOG6ZKXQ4.

Family and domain databases

Gene3Di3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00071. LepA.
InterProiIPR006297. EF-4.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR031157. G_TR_CS.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q68X95-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNQKYIRNF SIIAHIDHGK STLADRLIEH CGGLNAREMS QQVLDSMDIE
60 70 80 90 100
KERGITIKAQ TVRLVYKAKN GNTYYLNLMD TPGHVDFSYE VSRSLAACEG
110 120 130 140 150
SLLVVDSTQG VEAQTLANVY QAIANDHEIV PVLNKIDLAA SEPEHVKKQI
160 170 180 190 200
EDIIGIDASE AVLISAKNGI GIDSVLEAII NKLPSPKESS TDTLKALLVD
210 220 230 240 250
SWYDPYLGVV ILVRIIDGTL RKNMRVKMIG TNSVYTVEHV GFFTPKKHIS
260 270 280 290 300
DVLYAGEIGF FTASIKRVSD CKVGDTITDE KKSCEQALPG FKPNIPVVFC
310 320 330 340 350
GFYPTDSAEF EHLKDSLAKL RLNDASFEYE MESSSALGVG FRCGFLGLLH
360 370 380 390 400
LEIIQERLSR EFNLDLITTA PSVIYKIYML DGESLEIHNP ADLPDLQKIA
410 420 430 440 450
SMEEPWIKAT IMVPDEFIGT VLSLCKEKRG IQLDHSYISN RAKIVYKLPL
460 470 480 490 500
NEIVYDFYDR LKSCSKGYAS FEWQMDVYEL SDLVNLRILV NGEVVDALST
510 520 530 540 550
IVHRSRAEQR GRALCVRLKD LIPRQQIDIA IQASVGNRII ARETIKALRK
560 570 580 590 600
DVLSKCYGGD ISRKRKLLEK QKAGKKKMRQ YGNIEIPQSA FIAALQIGGE
Length:600
Mass (Da):67,101
Last modified:October 11, 2004 - v1
Checksum:iB512FBCEA0D96FAD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017197 Genomic DNA. Translation: AAU03747.1.
RefSeqiWP_011190732.1. NC_006142.1.
YP_067229.1. NC_006142.1.

Genome annotation databases

EnsemblBacteriaiAAU03747; AAU03747; RT0266.
KEGGirty:RT0266.
PATRICi17909718. VBIRicTyp34752_0275.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017197 Genomic DNA. Translation: AAU03747.1.
RefSeqiWP_011190732.1. NC_006142.1.
YP_067229.1. NC_006142.1.

3D structure databases

ProteinModelPortaliQ68X95.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi257363.RT0266.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU03747; AAU03747; RT0266.
KEGGirty:RT0266.
PATRICi17909718. VBIRicTyp34752_0275.

Phylogenomic databases

eggNOGiCOG0481.
HOGENOMiHOG000020624.
KOiK03596.
OMAiFAGIYPV.
OrthoDBiEOG6ZKXQ4.

Enzyme and pathway databases

BioCyciRTYP257363:GJEQ-279-MONOMER.

Family and domain databases

Gene3Di3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00071. LepA.
InterProiIPR006297. EF-4.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR031157. G_TR_CS.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-144 / Wilmington.

Entry informationi

Entry nameiLEPA_RICTY
AccessioniPrimary (citable) accession number: Q68X95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: October 11, 2004
Last modified: June 24, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia typhi
    Rickettsia typhi (strain Wilmington): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.