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Q68X95

- LEPA_RICTY

UniProt

Q68X95 - LEPA_RICTY

Protein

Elongation factor 4

Gene

lepA

Organism
Rickettsia typhi (strain ATCC VR-144 / Wilmington)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.UniRule annotation

    Catalytic activityi

    GTP + H2O = GDP + phosphate.UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi17 – 226GTPUniRule annotation
    Nucleotide bindingi134 – 1374GTPUniRule annotation

    GO - Molecular functioni

    1. GTPase activity Source: UniProtKB-HAMAP
    2. GTP binding Source: UniProtKB-HAMAP
    3. ribosome binding Source: UniProtKB-HAMAP
    4. translation elongation factor activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. positive regulation of translation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciRTYP257363:GJEQ-279-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Elongation factor 4UniRule annotation (EC:3.6.5.n1UniRule annotation)
    Short name:
    EF-4UniRule annotation
    Alternative name(s):
    Ribosomal back-translocase LepAUniRule annotation
    Gene namesi
    Name:lepAUniRule annotation
    Ordered Locus Names:RT0266
    OrganismiRickettsia typhi (strain ATCC VR-144 / Wilmington)
    Taxonomic identifieri257363 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
    ProteomesiUP000000604: Chromosome

    Subcellular locationi

    Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 600600Elongation factor 4PRO_0000176335Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi257363.RT0266.

    Structurei

    3D structure databases

    ProteinModelPortaliQ68X95.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 187183tr-type GAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0481.
    HOGENOMiHOG000020624.
    KOiK03596.
    OMAiCHIHART.
    OrthoDBiEOG6ZKXQ4.

    Family and domain databases

    Gene3Di3.30.70.240. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPiMF_00071. LepA.
    InterProiIPR006297. EF-4.
    IPR000795. EF_GTP-bd_dom.
    IPR009022. EFG_III-V.
    IPR000640. EFG_V.
    IPR013842. LepA_GTP-bd_C.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view]
    PfamiPF00679. EFG_C. 1 hit.
    PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF06421. LepA_C. 1 hit.
    [Graphical view]
    PRINTSiPR00315. ELONGATNFCT.
    SMARTiSM00838. EFG_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    SSF54980. SSF54980. 2 hits.
    TIGRFAMsiTIGR01393. lepA. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEiPS00301. G_TR_1. 1 hit.
    PS51722. G_TR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q68X95-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNNQKYIRNF SIIAHIDHGK STLADRLIEH CGGLNAREMS QQVLDSMDIE    50
    KERGITIKAQ TVRLVYKAKN GNTYYLNLMD TPGHVDFSYE VSRSLAACEG 100
    SLLVVDSTQG VEAQTLANVY QAIANDHEIV PVLNKIDLAA SEPEHVKKQI 150
    EDIIGIDASE AVLISAKNGI GIDSVLEAII NKLPSPKESS TDTLKALLVD 200
    SWYDPYLGVV ILVRIIDGTL RKNMRVKMIG TNSVYTVEHV GFFTPKKHIS 250
    DVLYAGEIGF FTASIKRVSD CKVGDTITDE KKSCEQALPG FKPNIPVVFC 300
    GFYPTDSAEF EHLKDSLAKL RLNDASFEYE MESSSALGVG FRCGFLGLLH 350
    LEIIQERLSR EFNLDLITTA PSVIYKIYML DGESLEIHNP ADLPDLQKIA 400
    SMEEPWIKAT IMVPDEFIGT VLSLCKEKRG IQLDHSYISN RAKIVYKLPL 450
    NEIVYDFYDR LKSCSKGYAS FEWQMDVYEL SDLVNLRILV NGEVVDALST 500
    IVHRSRAEQR GRALCVRLKD LIPRQQIDIA IQASVGNRII ARETIKALRK 550
    DVLSKCYGGD ISRKRKLLEK QKAGKKKMRQ YGNIEIPQSA FIAALQIGGE 600
    Length:600
    Mass (Da):67,101
    Last modified:October 11, 2004 - v1
    Checksum:iB512FBCEA0D96FAD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017197 Genomic DNA. Translation: AAU03747.1.
    RefSeqiYP_067229.1. NC_006142.1.

    Genome annotation databases

    EnsemblBacteriaiAAU03747; AAU03747; RT0266.
    GeneIDi2958556.
    KEGGirty:RT0266.
    PATRICi17909718. VBIRicTyp34752_0275.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017197 Genomic DNA. Translation: AAU03747.1 .
    RefSeqi YP_067229.1. NC_006142.1.

    3D structure databases

    ProteinModelPortali Q68X95.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 257363.RT0266.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAU03747 ; AAU03747 ; RT0266 .
    GeneIDi 2958556.
    KEGGi rty:RT0266.
    PATRICi 17909718. VBIRicTyp34752_0275.

    Phylogenomic databases

    eggNOGi COG0481.
    HOGENOMi HOG000020624.
    KOi K03596.
    OMAi CHIHART.
    OrthoDBi EOG6ZKXQ4.

    Enzyme and pathway databases

    BioCyci RTYP257363:GJEQ-279-MONOMER.

    Family and domain databases

    Gene3Di 3.30.70.240. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPi MF_00071. LepA.
    InterProi IPR006297. EF-4.
    IPR000795. EF_GTP-bd_dom.
    IPR009022. EFG_III-V.
    IPR000640. EFG_V.
    IPR013842. LepA_GTP-bd_C.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view ]
    Pfami PF00679. EFG_C. 1 hit.
    PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF06421. LepA_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00315. ELONGATNFCT.
    SMARTi SM00838. EFG_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    SSF54980. SSF54980. 2 hits.
    TIGRFAMsi TIGR01393. lepA. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEi PS00301. G_TR_1. 1 hit.
    PS51722. G_TR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC VR-144 / Wilmington.

    Entry informationi

    Entry nameiLEPA_RICTY
    AccessioniPrimary (citable) accession number: Q68X95
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2005
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Rickettsia typhi
      Rickettsia typhi (strain Wilmington): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3