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Protein

Elongation factor 4

Gene

lepA

Organism
Rickettsia typhi (strain ATCC VR-144 / Wilmington)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.UniRule annotation

Catalytic activityi

GTP + H2O = GDP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 22GTPUniRule annotation6
Nucleotide bindingi134 – 137GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRTYP257363:G1G0L-271-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 4UniRule annotation (EC:3.6.5.n1UniRule annotation)
Short name:
EF-4UniRule annotation
Alternative name(s):
Ribosomal back-translocase LepAUniRule annotation
Gene namesi
Name:lepAUniRule annotation
Ordered Locus Names:RT0266
OrganismiRickettsia typhi (strain ATCC VR-144 / Wilmington)
Taxonomic identifieri257363 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
Proteomesi
  • UP000000604 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001763351 – 600Elongation factor 4Add BLAST600

Interactioni

Protein-protein interaction databases

STRINGi257363.RT0266

Structurei

3D structure databases

ProteinModelPortaliQ68X95
SMRiQ68X95
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 187tr-type GAdd BLAST183

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4S Bacteria
COG0481 LUCA
HOGENOMiHOG000020624
KOiK03596
OMAiALIFDSW
OrthoDBiPOG091H02HW

Family and domain databases

CDDicd03709 lepA_C, 1 hit
Gene3Di3.30.70.2570, 1 hit
HAMAPiMF_00071 LepA, 1 hit
InterProiView protein in InterPro
IPR006297 EF-4
IPR035647 EFG_III/V
IPR000640 EFG_V-like
IPR004161 EFTu-like_2
IPR031157 G_TR_CS
IPR038363 LepA_C_sf
IPR013842 LepA_CTD
IPR035654 LepA_IV
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
PfamiView protein in Pfam
PF00679 EFG_C, 1 hit
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF06421 LepA_C, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF52540 SSF52540, 1 hit
SSF54980 SSF54980, 2 hits
TIGRFAMsiTIGR01393 lepA, 1 hit
TIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q68X95-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNQKYIRNF SIIAHIDHGK STLADRLIEH CGGLNAREMS QQVLDSMDIE
60 70 80 90 100
KERGITIKAQ TVRLVYKAKN GNTYYLNLMD TPGHVDFSYE VSRSLAACEG
110 120 130 140 150
SLLVVDSTQG VEAQTLANVY QAIANDHEIV PVLNKIDLAA SEPEHVKKQI
160 170 180 190 200
EDIIGIDASE AVLISAKNGI GIDSVLEAII NKLPSPKESS TDTLKALLVD
210 220 230 240 250
SWYDPYLGVV ILVRIIDGTL RKNMRVKMIG TNSVYTVEHV GFFTPKKHIS
260 270 280 290 300
DVLYAGEIGF FTASIKRVSD CKVGDTITDE KKSCEQALPG FKPNIPVVFC
310 320 330 340 350
GFYPTDSAEF EHLKDSLAKL RLNDASFEYE MESSSALGVG FRCGFLGLLH
360 370 380 390 400
LEIIQERLSR EFNLDLITTA PSVIYKIYML DGESLEIHNP ADLPDLQKIA
410 420 430 440 450
SMEEPWIKAT IMVPDEFIGT VLSLCKEKRG IQLDHSYISN RAKIVYKLPL
460 470 480 490 500
NEIVYDFYDR LKSCSKGYAS FEWQMDVYEL SDLVNLRILV NGEVVDALST
510 520 530 540 550
IVHRSRAEQR GRALCVRLKD LIPRQQIDIA IQASVGNRII ARETIKALRK
560 570 580 590 600
DVLSKCYGGD ISRKRKLLEK QKAGKKKMRQ YGNIEIPQSA FIAALQIGGE
Length:600
Mass (Da):67,101
Last modified:October 11, 2004 - v1
Checksum:iB512FBCEA0D96FAD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017197 Genomic DNA Translation: AAU03747.1
RefSeqiWP_011190732.1, NC_006142.1

Genome annotation databases

EnsemblBacteriaiAAU03747; AAU03747; RT0266
KEGGirty:RT0266

Similar proteinsi

Entry informationi

Entry nameiLEPA_RICTY
AccessioniPrimary (citable) accession number: Q68X95
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: October 11, 2004
Last modified: March 28, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia typhi
    Rickettsia typhi (strain Wilmington): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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