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Q68X45

- TOP1_RICTY

UniProt

Q68X45 - TOP1_RICTY

Protein

DNA topoisomerase 1

Gene

topA

Organism
Rickettsia typhi (strain ATCC VR-144 / Wilmington)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

    Cofactori

    Magnesium. Binds two Mg2+ per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi7 – 71Magnesium 1; catalyticUniRule annotation
    Sitei31 – 311Interaction with DNAUniRule annotation
    Metal bindingi80 – 801Magnesium 1; catalyticUniRule annotation
    Metal bindingi80 – 801Magnesium 2UniRule annotation
    Metal bindingi82 – 821Magnesium 2UniRule annotation
    Sitei142 – 1421Interaction with DNAUniRule annotation
    Sitei143 – 1431Interaction with DNAUniRule annotation
    Sitei146 – 1461Interaction with DNAUniRule annotation
    Sitei158 – 1581Interaction with DNAUniRule annotation
    Active sitei304 – 3041O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
    Sitei306 – 3061Interaction with DNAUniRule annotation
    Sitei499 – 4991Interaction with DNAUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri600 – 62728C4-typeAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA topological change Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciRTYP257363:GJEQ-332-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
    Alternative name(s):
    DNA topoisomerase IUniRule annotation
    Omega-protein
    Relaxing enzyme
    Swivelase
    Untwisting enzyme
    Gene namesi
    Name:topAUniRule annotation
    Ordered Locus Names:RT0317
    OrganismiRickettsia typhi (strain ATCC VR-144 / Wilmington)
    Taxonomic identifieri257363 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
    ProteomesiUP000000604: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 779779DNA topoisomerase 1PRO_0000273106Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi257363.RT0317.

    Structurei

    3D structure databases

    ProteinModelPortaliQ68X45.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 111111ToprimUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni166 – 1716Interaction with DNAUniRule annotation

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.UniRule annotation
    Contains 1 Toprim domain.UniRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri600 – 62728C4-typeAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG1754.
    HOGENOMiHOG000004020.
    KOiK03168.
    OMAiCERELEI.
    OrthoDBiEOG6S7XQ9.

    Family and domain databases

    Gene3Di1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPiMF_00952. Topoisom_1_prok.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR005733. TopoI_bac-type.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 1 hit.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 2 hits.
    TIGRFAMsiTIGR01051. topA_bact. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q68X45-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLVIVESPA KAKTINKYLG DEFKVIASFG HIRDLPSKKG SVLPDKNFAM    50
    EYDISDKASK YVDAIVKYAR KAEAVYLATD PDREGESISW HVAEVIKEKN 100
    KVESDDFFKR VAFNEITKKA IIHAVENPRK LDTNLVNAQQ ARRALDYLVG 150
    FTLSPLLWRK LPGCKSAGRV QSVALRLICD REDEIERFKA EEYWDISLKM 200
    QNSNNELFTA KLTHVNDQKL KKFSIINEKE AKDLTRKLKL QKFYVEKIEK 250
    KQQKRQPQPP FITSSLQQEA ARKLGFSAKK TMQIAQKLYE GVDIGKETIG 300
    LITYMRTDGV TLSNDAIADI RKLIDKNYGN KYLPINPRIY QSKVKNAQEA 350
    HEAIRPTNIT YTPDNLKQKL EKDYYKLYEL IWQRTIACQM ENVIMDLVIA 400
    NLASENKEYL AKANGSIIAF DGFYKVYRES LDDEDEEDNK MLPPLKAQEH 450
    IKTKEVIPNK HFTEPPPRYS EASLVKKLEE LGIGRPSTYA SILSVLQDRK 500
    YVTLEKKRFI PEELGRLVTV FLVGFFKKYV EYDFTAGLEN ELDEIAAGKL 550
    EWKTSLNNFW SGFNNNIESV NEQKITEIIN YLQKALDYHL FGENKESKVC 600
    PSCKTGQLSL KLGKFGAFLA CSNYPECTFK KSIVSGNDNN EDEGNLSTIL 650
    NDNKILGTDK DGVEIYLKTG PYGPYIQLGE QCGKVKPKRT PVPTNLKQSE 700
    ITLEVALKLL SLPLKIGIHK DSGEEIIIGY SKFGPYIKYM CKFISVPKKY 750
    DFLNLNLDDA IKLIENNKAK LEKKHRSMV 779
    Length:779
    Mass (Da):89,083
    Last modified:October 11, 2004 - v1
    Checksum:iD300A268969CEA89
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017197 Genomic DNA. Translation: AAU03797.1.
    RefSeqiYP_067279.1. NC_006142.1.

    Genome annotation databases

    EnsemblBacteriaiAAU03797; AAU03797; RT0317.
    GeneIDi2958716.
    KEGGirty:RT0317.
    PATRICi17909828. VBIRicTyp34752_0329.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017197 Genomic DNA. Translation: AAU03797.1 .
    RefSeqi YP_067279.1. NC_006142.1.

    3D structure databases

    ProteinModelPortali Q68X45.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 257363.RT0317.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAU03797 ; AAU03797 ; RT0317 .
    GeneIDi 2958716.
    KEGGi rty:RT0317.
    PATRICi 17909828. VBIRicTyp34752_0329.

    Phylogenomic databases

    eggNOGi COG1754.
    HOGENOMi HOG000004020.
    KOi K03168.
    OMAi CERELEI.
    OrthoDBi EOG6S7XQ9.

    Enzyme and pathway databases

    BioCyci RTYP257363:GJEQ-332-MONOMER.

    Family and domain databases

    Gene3Di 1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPi MF_00952. Topoisom_1_prok.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR005733. TopoI_bac-type.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 1 hit.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 2 hits.
    TIGRFAMsi TIGR01051. topA_bact. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC VR-144 / Wilmington.

    Entry informationi

    Entry nameiTOP1_RICTY
    AccessioniPrimary (citable) accession number: Q68X45
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2007
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Rickettsia typhi
      Rickettsia typhi (strain Wilmington): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3