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Q68X04 (SYK_RICTY) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lysine--tRNA ligase

EC=6.1.1.6
Alternative name(s):
Lysyl-tRNA synthetase
Short name=LysRS
Gene names
Name:lysS
Ordered Locus Names:RT0359
OrganismRickettsia typhi (strain ATCC VR-144 / Wilmington) [Complete proteome] [HAMAP]
Taxonomic identifier257363 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group

Protein attributes

Sequence length526 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP-Rule MF_00177

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00177.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processlysyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

lysine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 526526Lysine--tRNA ligase HAMAP-Rule MF_00177
PRO_0000278076

Regions

Motif44 – 529"HIGH" region HAMAP-Rule MF_00177
Motif290 – 2945"KMSKS" region HAMAP-Rule MF_00177

Sites

Binding site2931ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q68X04 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 2A14E4023967A19F

FASTA52660,516
        10         20         30         40         50         60 
MSVIWKDAIR SNAWPFIEAK KILDSLNGKV PEKGYVLFET GYGPSGLPHI GTFAENARVV 

        70         80         90        100        110        120 
MVQKAFEQLS DIPTKLICFS DDMDGLRKVP SNIPNPEMVA QYMDMPLTSI PDPFGECKSY 

       130        140        150        160        170        180 
GHYMNAKLCT FLDKFGFQYE FYSSTNCYKA GLFDDMLIRV LEKYDEIMAL MLPTFREERK 

       190        200        210        220        230        240 
TTYAPFMPIC PKTGKVLQVP IEKWDARAGT VSYKDEDGND VEVPVTGGHC KLQWKPDFGM 

       250        260        270        280        290        300 
RWAALNVDYE MYGKDHLANS RLYSKICQIL GGKPPVQFCY ELFLDENGEK ISKSRGNSIS 

       310        320        330        340        350        360 
VDDWLKYASV ESIALFMYKN PARAKRLFFD VIPKNVDEYI TLNQKYHLEE DMVTRFANPV 

       370        380        390        400        410        420 
YHIHHGNVPK IETFGLTYSL LLNLTAVCNP SDKSVLWGFI TKYEPKATPN TSTYLDHLAE 

       430        440        450        460        470        480 
FAIRYYHDFV QIHKSYLVVS EKHKIILYDI LDMLSNITDQ TEEENIQKAM YDIGMKAGYK 

       490        500        510        520 
NLRYYFKDLY QILLGQNEGP RFGTFIKLYG VEATKKLVEE KLYPVA 

« Hide

References

[1]"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae."
McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. expand/collapse author list , Hong C., Yu X.-J., Walker D.H., Weinstock G.M.
J. Bacteriol. 186:5842-5855(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017197 Genomic DNA. Translation: AAU03838.1.
RefSeqYP_067320.1. NC_006142.1.

3D structure databases

ProteinModelPortalQ68X04.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING257363.RT0359.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU03838; AAU03838; RT0359.
GeneID2958659.
KEGGrty:RT0359.
PATRIC17909922. VBIRicTyp34752_0375.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1384.
HOGENOMHOG000140447.
KOK04566.
OMATMVRHAF.
OrthoDBEOG6WHNM6.
ProtClustDBPRK00750.

Enzyme and pathway databases

BioCycRTYP257363:GJEQ-376-MONOMER.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00177. Lys_tRNA_synth_class1.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR002904. Lys-tRNA-ligase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF01921. tRNA-synt_1f. 1 hit.
[Graphical view]
SUPFAMSSF48163. SSF48163. 1 hit.
TIGRFAMsTIGR00467. lysS_arch. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYK_RICTY
AccessionPrimary (citable) accession number: Q68X04
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: October 11, 2004
Last modified: February 19, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia typhi

Rickettsia typhi (strain Wilmington): entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries