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Q68WV8 (SYL_RICTY) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:RT0407
OrganismRickettsia typhi (strain ATCC VR-144 / Wilmington) [Complete proteome] [HAMAP]
Taxonomic identifier257363 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group

Protein attributes

Sequence length828 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 828828Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152076

Regions

Motif36 – 4611"HIGH" region HAMAP-Rule MF_00049
Motif595 – 5995"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5981ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q68WV8 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 5981B26469952B26

FASTA82896,495
        10         20         30         40         50         60 
MHKIEQKWQK IWKEEKAFQV SNDSSKPKYY VLEMLPYPSG KIHIGHIRNY SIGDVIARFM 

        70         80         90        100        110        120 
TMQGFNVLHP MGWDAFGLPA ENAAIQNNSR PQDWTYSNIE YMKKQLQSMG FSYDWTREIN 

       130        140        150        160        170        180 
SCDPQYYKHE QKFFLELYDR DLVYQKESLV NWDPVDNTVL ANEQVVNGRG WRSGAIIEKR 

       190        200        210        220        230        240 
YLNQWFLKIT NYAEELLNEI QNLKDWPEAV RVMQEEWIGK STGVNFHFKI KYHENSTIEV 

       250        260        270        280        290        300 
FSTKPETIFG ASFIGIAFNH PIIEQLIFKT DEIANFITKC SYITKSSELE KSEKEGVFTG 

       310        320        330        340        350        360 
LYVIHPFDAN IILPVIITNF VLMDYGTGAI FGCPAHDKRD HELAMKMNLP IKKVIGTDNT 

       370        380        390        400        410        420 
QEEVLINSDW LNGLTSSEAK QKVISKFETL GIGKRSVNYR LKDWGISRQR FWGCPIPIIY 

       430        440        450        460        470        480 
CETCGIVPVP YKDLPVTLPD DVSFDNNYNP LEHHPSWKHV NCPKCDNHAI RETDTFDTFF 

       490        500        510        520        530        540 
ESSWYFTRYC NNNAVEMTDS KACNYWLPVD KYIGGIEHAV MHLLYARFFT KLMNEHHYVS 

       550        560        570        580        590        600 
IREPFKGLFT QGMVLHATYK DENNNWLYPA EVVKNGNEFF HKETNTRVVQ GRIEKMSKSK 

       610        620        630        640        650        660 
KNIIDLETIQ EQYSADAIRL FVLSDSPPEK DLQWSASGIE GCSRFIHKLD NMFEVISSLK 

       670        680        690        700        710        720 
DDMNNEINTE LNRLIHFTIK HVADDIKTFS LNRAIARMRT LTNAIYCEIS KDKIDVRTIK 

       730        740        750        760        770        780 
YGFNVLIQLL NPFIPHITEE IWQKLGNKER LYKSVFAEFD DSMLEFSTYI MAVQVNGKLR 

       790        800        810        820 
DTYEFNTDIS EDEVKQITVN LPKIQKFLAG KEPNKIILVP RKIVNIII 

« Hide

References

[1]"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae."
McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. expand/collapse author list , Hong C., Yu X.-J., Walker D.H., Weinstock G.M.
J. Bacteriol. 186:5842-5855(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017197 Genomic DNA. Translation: AAU03884.1.
RefSeqYP_067366.1. NC_006142.1.

3D structure databases

ProteinModelPortalQ68WV8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING257363.RT0407.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU03884; AAU03884; RT0407.
GeneID2958622.
KEGGrty:RT0407.
PATRIC17910048. VBIRicTyp34752_0436.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycRTYP257363:GJEQ-429-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_RICTY
AccessionPrimary (citable) accession number: Q68WV8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: October 11, 2004
Last modified: April 16, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia typhi

Rickettsia typhi (strain Wilmington): entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries