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Protein

Cysteine desulfurase IscS

Gene

iscS

Organism
Rickettsia typhi (strain ATCC VR-144 / Wilmington)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins.UniRule annotation

Catalytic activityi

L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor.UniRule annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei160 – 1601Pyridoxal phosphateUniRule annotation
Binding sitei188 – 1881Pyridoxal phosphateUniRule annotation
Binding sitei248 – 2481Pyridoxal phosphateUniRule annotation
Active sitei334 – 3341Cysteine persulfide intermediateUniRule annotation
Metal bindingi334 – 3341Iron-sulfur (2Fe-2S); via persulfide group; shared with IscUUniRule annotation

GO - Molecular functioni

  1. cysteine desulfurase activity Source: UniProtKB-HAMAP
  2. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. cysteine metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding, Pyridoxal phosphate

Enzyme and pathway databases

BioCyciRTYP257363:GJEQ-495-MONOMER.
UniPathwayiUPA00266.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine desulfurase IscSUniRule annotation (EC:2.8.1.7UniRule annotation)
Gene namesi
Name:iscSUniRule annotation
Ordered Locus Names:RT0473
OrganismiRickettsia typhi (strain ATCC VR-144 / Wilmington)
Taxonomic identifieri257363 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
ProteomesiUP000000604: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 410410Cysteine desulfurase IscSPRO_0000277975Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei211 – 2111N6-(pyridoxal phosphate)lysineUniRule annotation

Interactioni

Subunit structurei

Homodimer. Forms a heterotetramer with IscU, interacts with other sulfur acceptors.UniRule annotation

Protein-protein interaction databases

STRINGi257363.RT0473.

Structurei

3D structure databases

ProteinModelPortaliQ68WP6.
SMRiQ68WP6. Positions 9-398.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni80 – 812Pyridoxal phosphate bindingUniRule annotation
Regioni208 – 2103Pyridoxal phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1104.
HOGENOMiHOG000017510.
KOiK04487.
OMAiAEKMMQC.
OrthoDBiEOG62RSBK.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00331. Cys_desulf_IscS.
InterProiIPR000192. Aminotrans_V_dom.
IPR020578. Aminotrans_V_PyrdxlP_BS.
IPR010240. Cys_deSase.
IPR016454. Cysteine_dSase_NifS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF005572. NifS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR02006. IscS. 1 hit.
PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q68WP6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQQLKNLTL PIYMDYQSTT PIDPRVMEAM LPYFTTKFGN PHSRSHSFGW
60 70 80 90 100
EAENAVENAR SMIAKLIGAD SKEIIFTSGA TESNNLVIKG IAKFYGNKKN
110 120 130 140 150
HIITLVSEHK CVLNACRYLE QEGIKITYLP IKPNGIIDLE ILKNAITDQT
160 170 180 190 200
LLVSVMAVNN EIGVIQPLRE IGKICRERSV FFHSDIAQGF GKIPINVNEY
210 220 230 240 250
NIDLASISGH KIYGPKGIGA LYIRKKPRVR VTPLINGGGQ ERGMRSGTLP
260 270 280 290 300
TPLIVGFGIA AEISYNEMEK DAQHVNYLFD RFLNNIYSRI PEVYLNGDKD
310 320 330 340 350
QRYKGNLNLS FAGVEGESII LAIKDLAVSS GSACTSASLE PSYVLRSIGI
360 370 380 390 400
SEELAHTSIR FGIGRFTTEQ EIDYAVNLIC SKIDKLRKLS PLWEMMQEGI
410
DLKKIKWTAH
Length:410
Mass (Da):45,761
Last modified:October 11, 2004 - v1
Checksum:i053978B04BA24782
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017197 Genomic DNA. Translation: AAU03946.1.
RefSeqiWP_011190929.1. NC_006142.1.
YP_067428.1. NC_006142.1.

Genome annotation databases

EnsemblBacteriaiAAU03946; AAU03946; RT0473.
GeneIDi2958830.
KEGGirty:RT0473.
PATRICi17910188. VBIRicTyp34752_0502.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017197 Genomic DNA. Translation: AAU03946.1.
RefSeqiWP_011190929.1. NC_006142.1.
YP_067428.1. NC_006142.1.

3D structure databases

ProteinModelPortaliQ68WP6.
SMRiQ68WP6. Positions 9-398.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi257363.RT0473.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU03946; AAU03946; RT0473.
GeneIDi2958830.
KEGGirty:RT0473.
PATRICi17910188. VBIRicTyp34752_0502.

Phylogenomic databases

eggNOGiCOG1104.
HOGENOMiHOG000017510.
KOiK04487.
OMAiAEKMMQC.
OrthoDBiEOG62RSBK.

Enzyme and pathway databases

UniPathwayiUPA00266.
BioCyciRTYP257363:GJEQ-495-MONOMER.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00331. Cys_desulf_IscS.
InterProiIPR000192. Aminotrans_V_dom.
IPR020578. Aminotrans_V_PyrdxlP_BS.
IPR010240. Cys_deSase.
IPR016454. Cysteine_dSase_NifS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF005572. NifS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR02006. IscS. 1 hit.
PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-144 / Wilmington.

Entry informationi

Entry nameiISCS_RICTY
AccessioniPrimary (citable) accession number: Q68WP6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: October 11, 2004
Last modified: March 4, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Rickettsia typhi
    Rickettsia typhi (strain Wilmington): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.