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Q68WP6

- ISCS_RICTY

UniProt

Q68WP6 - ISCS_RICTY

Protein

Cysteine desulfurase IscS

Gene

iscS

Organism
Rickettsia typhi (strain ATCC VR-144 / Wilmington)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins.UniRule annotation

    Catalytic activityi

    L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor.UniRule annotation

    Cofactori

    Pyridoxal phosphate.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei334 – 3341Cysteine persulfide intermediateUniRule annotation

    GO - Molecular functioni

    1. cysteine desulfurase activity Source: UniProtKB-HAMAP
    2. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. cysteine metabolic process Source: InterPro

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciRTYP257363:GJEQ-495-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cysteine desulfurase IscSUniRule annotation (EC:2.8.1.7UniRule annotation)
    Gene namesi
    Name:iscSUniRule annotation
    Ordered Locus Names:RT0473
    OrganismiRickettsia typhi (strain ATCC VR-144 / Wilmington)
    Taxonomic identifieri257363 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
    ProteomesiUP000000604: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 410410Cysteine desulfurase IscSPRO_0000277975Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei211 – 2111N6-(pyridoxal phosphate)lysineUniRule annotation

    Interactioni

    Subunit structurei

    Homodimer. Forms a heterotetramer with IscU, interacts with other sulfur acceptors.UniRule annotation

    Protein-protein interaction databases

    STRINGi257363.RT0473.

    Structurei

    3D structure databases

    ProteinModelPortaliQ68WP6.
    SMRiQ68WP6. Positions 9-398.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1104.
    HOGENOMiHOG000017510.
    KOiK04487.
    OMAiWGWEAEA.
    OrthoDBiEOG62RSBK.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    HAMAPiMF_00331. Cys_desulf_IscS.
    InterProiIPR000192. Aminotrans_V/Cys_dSase.
    IPR020578. Aminotrans_V_PyrdxlP_BS.
    IPR010240. Cys_deSase.
    IPR016454. Cysteine_dSase_NifS.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00266. Aminotran_5. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005572. NifS. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR02006. IscS. 1 hit.
    PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q68WP6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNQQLKNLTL PIYMDYQSTT PIDPRVMEAM LPYFTTKFGN PHSRSHSFGW    50
    EAENAVENAR SMIAKLIGAD SKEIIFTSGA TESNNLVIKG IAKFYGNKKN 100
    HIITLVSEHK CVLNACRYLE QEGIKITYLP IKPNGIIDLE ILKNAITDQT 150
    LLVSVMAVNN EIGVIQPLRE IGKICRERSV FFHSDIAQGF GKIPINVNEY 200
    NIDLASISGH KIYGPKGIGA LYIRKKPRVR VTPLINGGGQ ERGMRSGTLP 250
    TPLIVGFGIA AEISYNEMEK DAQHVNYLFD RFLNNIYSRI PEVYLNGDKD 300
    QRYKGNLNLS FAGVEGESII LAIKDLAVSS GSACTSASLE PSYVLRSIGI 350
    SEELAHTSIR FGIGRFTTEQ EIDYAVNLIC SKIDKLRKLS PLWEMMQEGI 400
    DLKKIKWTAH 410
    Length:410
    Mass (Da):45,761
    Last modified:October 11, 2004 - v1
    Checksum:i053978B04BA24782
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017197 Genomic DNA. Translation: AAU03946.1.
    RefSeqiWP_011190929.1. NC_006142.1.
    YP_067428.1. NC_006142.1.

    Genome annotation databases

    EnsemblBacteriaiAAU03946; AAU03946; RT0473.
    GeneIDi2958830.
    KEGGirty:RT0473.
    PATRICi17910188. VBIRicTyp34752_0502.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017197 Genomic DNA. Translation: AAU03946.1 .
    RefSeqi WP_011190929.1. NC_006142.1.
    YP_067428.1. NC_006142.1.

    3D structure databases

    ProteinModelPortali Q68WP6.
    SMRi Q68WP6. Positions 9-398.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 257363.RT0473.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAU03946 ; AAU03946 ; RT0473 .
    GeneIDi 2958830.
    KEGGi rty:RT0473.
    PATRICi 17910188. VBIRicTyp34752_0502.

    Phylogenomic databases

    eggNOGi COG1104.
    HOGENOMi HOG000017510.
    KOi K04487.
    OMAi WGWEAEA.
    OrthoDBi EOG62RSBK.

    Enzyme and pathway databases

    BioCyci RTYP257363:GJEQ-495-MONOMER.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    HAMAPi MF_00331. Cys_desulf_IscS.
    InterProi IPR000192. Aminotrans_V/Cys_dSase.
    IPR020578. Aminotrans_V_PyrdxlP_BS.
    IPR010240. Cys_deSase.
    IPR016454. Cysteine_dSase_NifS.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF00266. Aminotran_5. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005572. NifS. 1 hit.
    SUPFAMi SSF53383. SSF53383. 1 hit.
    TIGRFAMsi TIGR02006. IscS. 1 hit.
    PROSITEi PS00595. AA_TRANSFER_CLASS_5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC VR-144 / Wilmington.

    Entry informationi

    Entry nameiISCS_RICTY
    AccessioniPrimary (citable) accession number: Q68WP6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 20, 2007
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Rickettsia typhi
      Rickettsia typhi (strain Wilmington): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3