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Q68WP6 (ISCS_RICTY) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Cysteine desulfurase
EC=2.8.1.7
Gene names
Name:iscS
Ordered Locus Names:RT0473
OrganismRickettsia typhi (strain ATCC VR-144 / Wilmington) [Complete proteome] [HAMAP]
Taxonomic identifier257363 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group ›

Protein attributes

Sequence length410 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the removal of elemental sulfur from cysteine to produce alanine By similarity. HAMAP-Rule MF_00331

Catalytic activity

L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor. HAMAP-Rule MF_00331

Cofactor

Pyridoxal phosphate By similarity.

Sequence similarities

Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.

Ontologies

Keywords
   LigandPyridoxal phosphate
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcysteine metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncysteine desulfurase activity

Inferred from electronic annotation. Source: HAMAP

pyridoxal phosphate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 410410Cysteine desulfurase HAMAP-Rule MF_00331
PRO_0000277975

Sites

Active site3341Cysteine persulfide intermediate By similarity

Amino acid modifications

Modified residue2111N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q68WP6 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 053978B04BA24782

FASTA41045,761
        10         20         30         40         50         60 
MNQQLKNLTL PIYMDYQSTT PIDPRVMEAM LPYFTTKFGN PHSRSHSFGW EAENAVENAR 

        70         80         90        100        110        120 
SMIAKLIGAD SKEIIFTSGA TESNNLVIKG IAKFYGNKKN HIITLVSEHK CVLNACRYLE 

       130        140        150        160        170        180 
QEGIKITYLP IKPNGIIDLE ILKNAITDQT LLVSVMAVNN EIGVIQPLRE IGKICRERSV 

       190        200        210        220        230        240 
FFHSDIAQGF GKIPINVNEY NIDLASISGH KIYGPKGIGA LYIRKKPRVR VTPLINGGGQ 

       250        260        270        280        290        300 
ERGMRSGTLP TPLIVGFGIA AEISYNEMEK DAQHVNYLFD RFLNNIYSRI PEVYLNGDKD 

       310        320        330        340        350        360 
QRYKGNLNLS FAGVEGESII LAIKDLAVSS GSACTSASLE PSYVLRSIGI SEELAHTSIR 

       370        380        390        400        410 
FGIGRFTTEQ EIDYAVNLIC SKIDKLRKLS PLWEMMQEGI DLKKIKWTAH

« Hide

References

[1]"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae."
McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. expand/collapse author list , Hong C., Yu X.-J., Walker D.H., Weinstock G.M.
J. Bacteriol. 186:5842-5855(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE017197 Genomic DNA. Translation: AAU03946.1.
RefSeqYP_067428.1. NC_006142.1.

3D structure databases

HSSPHSSP built from PDB template 1P3W based on UniProtKB P0A6B7.
ProteinModelPortalQ68WP6.
SMRQ68WP6. Positions 9-398.
ModBaseSearch...

Protein-protein interaction databases

STRING257363.RT0473.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU03946; AAU03946; RT0473.
GeneID2958830.
KEGGrty:RT0473.
PATRIC17910188. VBIRicTyp34752_0502.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1104.
HOGENOMHOG000017510.
KOK04487.
OMALWEMFKQ.
ProtClustDBPRK14012.

Enzyme and pathway databases

BioCycRTYP257363:GJEQ-495-MONOMER.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPMF_00331. Cys_desulf_aminotr_5.
InterProIPR000192. Aminotrans_V/Cys_dSase.
IPR020578. Aminotrans_V_PyrdxlP_BS.
IPR010240. Cys_deSase.
IPR016454. Cysteine_dSase_NifS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFPIRSF005572. NifS. 1 hit.
SUPFAMSSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMsTIGR02006. IscS. 1 hit.
PROSITEPS00595. AA_TRANSFER_CLASS_5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameISCS_RICTY
AccessionPrimary (citable) accession number: Q68WP6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: October 11, 2004
Last modified: May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Rickettsia typhi

Rickettsia typhi (strain Wilmington): entries and gene names

SIMILARITY comments

Index of protein domains and families

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