Reviewed, UniProtKB/Swiss-Prot Q68WH7 (FAD3_RICTY)

Names and origin

Protein names

Recommended name:
    Putative fatty acid oxidation complex trifunctional enzyme
Including the following 2 domains:
    1- Recommended name:
            3-hydroxyacyl-CoA dehydrogenase
              EC=1.1.1.35
    2- Recommended name:
            Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase
              EC=4.2.1.17
              EC=5.3.3.8

Gene names

Ordered Locus Names:

RT0547

Organism

Rickettsia typhi [Complete proteome] [HAMAP]

Taxonomic identifier

785 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	720 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH. (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O. (3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA.
Sequence similarities	In the N-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family. In the C-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Ligand	NAD
Molecular function	Isomerase Lyase Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-hydroxyacyl-CoA dehydrogenase activity Inferred from electronic annotation. Source: EC coenzyme binding Inferred from electronic annotation. Source: InterPro dodecenoyl-CoA delta-isomerase activity Inferred from electronic annotation. Source: EC enoyl-CoA hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 720	720	Putative fatty acid oxidation complex trifunctional enzyme		PRO_0000285699
Regions
	Region	1 – 384	384	3-hydroxyacyl-CoA dehydrogenase
	Region	453 – 720	268	Enoyl-CoA hydratase/isomerase

Sequences

	Sequence		Length	Mass (Da)
	Q68WH7-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 969CD34D859084C6	FASTA	720	81,410
	10 20 30 40 50 60 MQNEIKKVCV IGAGVMGSGI AALIANSSHR VVLLDILDKD SNDPNKIVKN AVKNLHRQKL 70 80 90 100 110 120 PPLSYPDKVN FITIGNLEHD LDLIKECNLV IEVIVEKLDI KHQLYNKIIP YLKEDTIIAS 130 140 150 160 170 180 NTSTLPLKKL KENLPNNIKS RFIITHFFNP PRYMELVELI IDNTIKDEVI EKISVFLTKI 190 200 210 220 230 240 LGKTIIKCND TPGFIANRVG CFLLELVAHK AIAQNLDFVT IDQIFSRCLG LPNTGIFGLY 250 260 270 280 290 300 DLIGHDVMKL ISSSLLSALP KSDDYHRIYF NTKVFDKMIE HNLIGRKGEG GFYRLSVSNG 310 320 330 340 350 360 KKIKEVINIS DLSYHPVQKV DISFNNLDEL LASDSIYSKF FSEIITEFYI YLTSLVPSVT 370 380 390 400 410 420 NNIYDIDATM KLGYSWHYGP FELLTIAVKN GWNSIIKNAD LMNISLPKYL ASKEYQKIDK 430 440 450 460 470 480 QKFNSKKDFL QESTIVLAND SANLIHYCEN LIFVITTKMN TLNHNVFYLL QEAVSKAENY 490 500 510 520 530 540 GKNLYIFPQG NNFSAGADLK LILSYIQDGN FDNLENLLKL GQQTMRYLKY SSVHIISCAR 550 560 570 580 590 600 GVALGGGCEL LLNSSYIVAN QELNAGLIEF GVGLIPGWSG VTEMFARSNG DKTKLIRNIK 610 620 630 640 650 660 NIIEQNKTSS ADYFKADYDV SNMQVNMNKH YILDEALKLN VSNKIVSIPH KIALPKINIV 670 680 690 700 710 720 SEIDTSKYND LQNKVLNKFQ NIIDKHNEIS EAELLTYERE IFLELAKEPQ TIEKLHAIVG

References

[1]

"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae." McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. , Hong C., Yu X.-J., Walker D.H., Weinstock G.M. J. Bacteriol. 186:5842-5855(2004) [PubMed: 15317790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017197 Genomic DNA. Translation: AAU04015.1.
RefSeq	YP_067497.1.
3D structure databases
HSSP	HSSP built from PDB template 3HDH based on UniProtKB P00348.
SMR	Q68WH7. Positions 457-718.
ModBase	Search...
Genome annotation databases
GeneID	2958739.
GenomeReviews	Gene locus RT0547 in contig AE017197_GR.
KEGG	rty:RT0547.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG729334.
OMA	FRTLDVV.
Enzyme and pathway databases
BioCyc	RTYP257363:RT0547-MONOMER.
BRENDA	1.1.1.35. 281221. 4.2.1.17. 281221. 5.3.3.8. 281221.
Family and domain databases
InterPro	IPR006176. 3-OHacyl-CoA_DH_NAD-bd. IPR006108. 3HC_DH_C. IPR008927. 6-PGluconate_DH_C-like. IPR001753. Crotonase_core. IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
Pfam	PF00725. 3HCDH. 1 hit. PF02737. 3HCDH_N. 1 hit. PF00378. ECH. 1 hit. [Graphical view]
PROSITE	PS00067. 3HCDH. False negative. PS00166. ENOYL_COA_HYDRATASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FAD3_RICTY

Accession

Primary (citable) accession number: Q68WH7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	October 11, 2004
Last modified:	January 19, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)