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Q68WC2

- SYI_RICTY

UniProt

Q68WC2 - SYI_RICTY

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Protein

Isoleucine--tRNA ligase

Gene
ileS, RT0606
Organism
Rickettsia typhi (strain ATCC VR-144 / Wilmington)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei627 – 6271ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciRTYP257363:GJEQ-637-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:RT0606
OrganismiRickettsia typhi (strain ATCC VR-144 / Wilmington)
Taxonomic identifieri257363 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
ProteomesiUP000000604: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10861086Isoleucine--tRNA ligaseUniRule annotationPRO_0000098561Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi257363.RT0606.

Structurei

3D structure databases

ProteinModelPortaliQ68WC2.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi53 – 6311"HIGH" regionUniRule annotationAdd
BLAST
Motifi624 – 6285"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiYETLYEC.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q68WC2-1 [UniParc]FASTAAdd to Basket

« Hide

MTNTKYYPDV SANVDFAAIE REILKFWQNN NIFQKSIDHR DGESEFIFYD     50
GPPFANGLPH YGHLLTGFIK DVYARYQTVK GKKVERRFGW DCHGLPAEMQ 100
SEKELGISGR LAITNFGIEK FNNHCRASVM QYASEWKQYV TRQARWVAFD 150
NAYKTMDKNF MESVLWAFKE LYNKGLLYES MRVMPYSWAC ETPLSNFETR 200
LDNSYRERTD KAITVSFMLN DITLFNSMIS QKLGMTGGDN FKEYRILAWT 250
TTPWTLPANL ALAVGSDIDY ALVNKNDVCY IIAASSVAKY AKELGLSGKE 300
NFEIIKGLKL QGLSYKPLFN YFENHPNSFK IFASNFVVEG EGTGIVHMAP 350
GFGEDDQILC ESKGIELVCP VDNSGKFTKE IPDLEGVQVF DANDKIIIKL 400
KEQGNWIKTE QYIHNYPHCW RTDTPLIYKA VPSWYVRVTQ FKDRMVELNQ 450
QINWIPHNVK DNLFGKWLEN ARDWSISRNR FWGTPLPVWK SDDPKYPRID 500
VYGSIEEIEQ DFGVKINDLH RPFIDELTRT NPDDPTGKSI MRRIDDVFDC 550
WFESGSMPYG QAHYPFENKK WFVEHFPADF IVEYSSQTRG WFYTLIVLST 600
ALFDRPPFLN CICHGVILDA TGQKLSKRLN NYADPLELFD RYGSDALRVT 650
MLSSNVVKGQ ELLIDKDGKM IFDTLRLFIK PIWNAYHFFT IYANADALKG 700
TLNFTSQNVL DIYILSKLKI AVNKIEESLD NFDTQTAYHA VSEFFEVLNN 750
WYIRRSRARF WKKEKDTDKQ NAYNTLYSCL ETMTIAMSAL VPMISEAIYQ 800
GLHNTAITQL NCLLLEGKHV VQNPMSGTQD YNTSVHLCNY PTLSDFEINH 850
ELVSTMDNVL DICSNSLFIR STKNIRVRQP LACITIISKH NNNLKDFEDL 900
IKDEINVKTV IYRDDLENYA HKKLSLNFAI LGKRLPHKMK AIIDASKKGE 950
WETSTLGLVI CGEILNSNEY KLVLEPHSHI KGTANFENNS SLLILDLELT 1000
SELIEEGYAR DIIRFIQHAR KEADFSITDK ILIEIISEFD LSKIIENYGD 1050
FIKEQTLGEF AKNFMPDYVS KVALENNLIQ LKVKRL 1086
Length:1,086
Mass (Da):125,447
Last modified:October 11, 2004 - v1
Checksum:i02F4A7D6A1934203
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017197 Genomic DNA. Translation: AAU04070.1.
RefSeqiYP_067552.1. NC_006142.1.

Genome annotation databases

EnsemblBacteriaiAAU04070; AAU04070; RT0606.
GeneIDi2958495.
KEGGirty:RT0606.
PATRICi17910454. VBIRicTyp34752_0627.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017197 Genomic DNA. Translation: AAU04070.1 .
RefSeqi YP_067552.1. NC_006142.1.

3D structure databases

ProteinModelPortali Q68WC2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 257363.RT0606.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAU04070 ; AAU04070 ; RT0606 .
GeneIDi 2958495.
KEGGi rty:RT0606.
PATRICi 17910454. VBIRicTyp34752_0627.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi YETLYEC.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci RTYP257363:GJEQ-637-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-144 / Wilmington.

Entry informationi

Entry nameiSYI_RICTY
AccessioniPrimary (citable) accession number: Q68WC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 11, 2004
Last modified: May 14, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Rickettsia typhi
    Rickettsia typhi (strain Wilmington): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi