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Q68W66 (RSMA_RICTY) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosomal RNA small subunit methyltransferase A

EC=2.1.1.182
Alternative name(s):
16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase
16S rRNA dimethyladenosine transferase
16S rRNA dimethylase
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase
Gene names
Name:rsmA
Synonyms:ksgA
Ordered Locus Names:RT0666
OrganismRickettsia typhi (strain ATCC VR-144 / Wilmington) [Complete proteome] [HAMAP]
Taxonomic identifier257363 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group

Protein attributes

Sequence length268 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits By similarity. HAMAP-Rule MF_00607

Catalytic activity

4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA. HAMAP-Rule MF_00607

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00607.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 268268Ribosomal RNA small subunit methyltransferase A HAMAP-Rule MF_00607
PRO_0000101596

Sites

Binding site231S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site251S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site501S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site721S-adenosyl-L-methionine By similarity
Binding site971S-adenosyl-L-methionine By similarity
Binding site1161S-adenosyl-L-methionine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q68W66 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 0060DB172444B909

FASTA26830,159
        10         20         30         40         50         60 
MLPSIAKHAA SHQINPLKKH GQNFIFDSSL CDKIIRASNI SENSKVIEIG PGVGGLTRSI 

        70         80         90        100        110        120 
LHKNPKSLTV IEIDERCIPL LNEIQGYYPN LNIIKQDVLK INLTDLIYDK VTVISNLPYH 

       130        140        150        160        170        180 
IGTELVIRLL KEAKLITNMI LMLQKEVVER ICAMPSTKAY GRLSVICQIV AKVEKCFDVA 

       190        200        210        220        230        240 
PTAFYPHPKV YSAIVKIIPL ENPPSIALIN KVEQITKLVF AGRRKMIKSS LRNLVPNIHE 

       250        260 
VLTQLKINNN DRAENLTPKD YLRIAMQL 

« Hide

References

[1]"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae."
McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. expand/collapse author list , Hong C., Yu X.-J., Walker D.H., Weinstock G.M.
J. Bacteriol. 186:5842-5855(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017197 Genomic DNA. Translation: AAU04126.1.
RefSeqYP_067608.1. NC_006142.1.

3D structure databases

ProteinModelPortalQ68W66.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING257363.RT0666.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU04126; AAU04126; RT0666.
GeneID2958653.
KEGGrty:RT0666.
PATRIC17910574. VBIRicTyp34752_0683.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0030.
HOGENOMHOG000227961.
KOK02528.
OMAHIPRKRF.
OrthoDBEOG66F08Z.
ProtClustDBPRK00274.

Enzyme and pathway databases

BioCycRTYP257363:GJEQ-698-MONOMER.

Family and domain databases

Gene3D1.10.8.100. 1 hit.
HAMAPMF_00607. 16SrRNA_methyltr_A.
InterProIPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR001737. rRNA_Ade_methylase_transferase.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
[Graphical view]
PANTHERPTHR11727. PTHR11727. 1 hit.
PfamPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTSM00650. rADc. 1 hit.
[Graphical view]
TIGRFAMsTIGR00755. ksgA. 1 hit.
PROSITEPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRSMA_RICTY
AccessionPrimary (citable) accession number: Q68W66
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: October 11, 2004
Last modified: April 16, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia typhi

Rickettsia typhi (strain Wilmington): entries and gene names