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Q68W27

- DNLJ_RICTY

UniProt

Q68W27 - DNLJ_RICTY

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Protein

DNA ligase

Gene

ligA

Organism
Rickettsia typhi (strain ATCC VR-144 / Wilmington)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.UniRule annotation

Catalytic activityi

NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)(n+m).UniRule annotation

Cofactori

Magnesium or manganese.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei121 – 1211NADUniRule annotation
Active sitei123 – 1231N6-AMP-lysine intermediateUniRule annotation
Binding sitei144 – 1441NADUniRule annotation
Binding sitei179 – 1791NADUniRule annotation
Binding sitei295 – 2951NADUniRule annotation
Binding sitei319 – 3191NADUniRule annotation
Metal bindingi413 – 4131ZincUniRule annotation
Metal bindingi416 – 4161ZincUniRule annotation
Metal bindingi431 – 4311ZincUniRule annotation
Metal bindingi437 – 4371ZincUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi40 – 445NADUniRule annotation
Nucleotide bindingi89 – 902NADUniRule annotation

GO - Molecular functioni

  1. DNA ligase (NAD+) activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA repair Source: UniProtKB-KW
  2. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciRTYP257363:GJEQ-743-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligaseUniRule annotation (EC:6.5.1.2UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [NAD(+)]UniRule annotation
Gene namesi
Name:ligAUniRule annotation
Synonyms:lig
Ordered Locus Names:RT0706
OrganismiRickettsia typhi (strain ATCC VR-144 / Wilmington)
Taxonomic identifieri257363 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
ProteomesiUP000000604: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 693693DNA ligasePRO_0000280948Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi257363.RT0706.

Structurei

3D structure databases

ProteinModelPortaliQ68W27.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini610 – 69384BRCTUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.UniRule annotation
Contains 1 BRCT domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0272.
HOGENOMiHOG000218458.
KOiK01972.
OMAiKEYYVDS.
OrthoDBiEOG6TTVM9.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.50.10190. 1 hit.
HAMAPiMF_01588. DNA_ligase_A.
InterProiIPR001357. BRCT_dom.
IPR018239. DNA_ligase_AS.
IPR001679. DNAligase.
IPR013839. DNAligase_adenylation.
IPR013840. DNAligase_N.
IPR012340. NA-bd_OB-fold.
IPR004150. NAD_DNA_ligase_OB.
IPR010994. RuvA_2-like.
IPR004149. Znf_DNAligase_C4.
[Graphical view]
PfamiPF00533. BRCT. 1 hit.
PF01653. DNA_ligase_aden. 1 hit.
PF03120. DNA_ligase_OB. 1 hit.
PF03119. DNA_ligase_ZBD. 1 hit.
[Graphical view]
PIRSFiPIRSF001604. LigA. 1 hit.
SMARTiSM00292. BRCT. 1 hit.
SM00532. LIGANc. 1 hit.
[Graphical view]
SUPFAMiSSF47781. SSF47781. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 1 hit.
TIGRFAMsiTIGR00575. dnlj. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS01055. DNA_LIGASE_N1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q68W27-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQNIDLISEK EAKKLLEELA DKIAMYNHAY YIEDNPLVSD SEYDQLFNLN
60 70 80 90 100
LKLENTFPHL VLSNSPSKKI GAKITNKFAK VIHQAPMLSL SNAFDEEDIR
110 120 130 140 150
DFLERIKNFL RINEFTPIFC EPKIDGLSFS AIYKHGLFIT GATRGDGYVG
160 170 180 190 200
EDITINIKTI KNFPHKIDNA PEFLEVRGEI YIEKQDFVNL NKEQEAQNRG
210 220 230 240 250
KFANPRNAAA GSIRQLDVAI TAQRPLKYFI YSGGVTEKNL ASTQEQLLAK
260 270 280 290 300
LKALSFSVNE ISKLASSEEE IFAFYEYLKT NRHNLPYEID GVVYKLNNFA
310 320 330 340 350
MQNRMGFIAR SPRFAIAHKF PAIIGQTKLL SITVQVGRTG MLTPVAELDP
360 370 380 390 400
LEIGGVVVSR ATLHNFQDIA RKDVRIKDYV FLQRAGDVIP QITGVDISKR
410 420 430 440 450
SNDTVKFDTP VFCPSCNSKL RYVSEDIIIR CDNGLNCPAQ NYERICHFVS
460 470 480 490 500
KNAMDIAGLG RKQVAFLIDK RLISNPLDIF FLKKKNETNL IRLENMDGWG
510 520 530 540 550
KKSVANLWQN IEKSQKISLQ RFIYALGIRH IGEQNAKLLA REFVSYNNFI
560 570 580 590 600
AQMELLSKND SDVYQKLNDL EGIGDKMLVD IIDFFYVKEN IQLIKKLGAV
610 620 630 640 650
LNIEDYKETR EQNILTGKIL VFTGSLKTIS RVEAKEIAEK LGAKVASSVS
660 670 680 690
SNTDLLIVGV NGGSKLKKAK ELNIKIIDEA EWLAFIKCLE KEV
Length:693
Mass (Da):78,483
Last modified:October 11, 2004 - v1
Checksum:i80BE9C9493DF658B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017197 Genomic DNA. Translation: AAU04165.1.
RefSeqiYP_067647.1. NC_006142.1.

Genome annotation databases

EnsemblBacteriaiAAU04165; AAU04165; RT0706.
GeneIDi2959338.
KEGGirty:RT0706.
PATRICi17910662. VBIRicTyp34752_0726.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017197 Genomic DNA. Translation: AAU04165.1 .
RefSeqi YP_067647.1. NC_006142.1.

3D structure databases

ProteinModelPortali Q68W27.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 257363.RT0706.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAU04165 ; AAU04165 ; RT0706 .
GeneIDi 2959338.
KEGGi rty:RT0706.
PATRICi 17910662. VBIRicTyp34752_0726.

Phylogenomic databases

eggNOGi COG0272.
HOGENOMi HOG000218458.
KOi K01972.
OMAi KEYYVDS.
OrthoDBi EOG6TTVM9.

Enzyme and pathway databases

BioCyci RTYP257363:GJEQ-743-MONOMER.

Family and domain databases

Gene3Di 2.40.50.140. 1 hit.
3.40.50.10190. 1 hit.
HAMAPi MF_01588. DNA_ligase_A.
InterProi IPR001357. BRCT_dom.
IPR018239. DNA_ligase_AS.
IPR001679. DNAligase.
IPR013839. DNAligase_adenylation.
IPR013840. DNAligase_N.
IPR012340. NA-bd_OB-fold.
IPR004150. NAD_DNA_ligase_OB.
IPR010994. RuvA_2-like.
IPR004149. Znf_DNAligase_C4.
[Graphical view ]
Pfami PF00533. BRCT. 1 hit.
PF01653. DNA_ligase_aden. 1 hit.
PF03120. DNA_ligase_OB. 1 hit.
PF03119. DNA_ligase_ZBD. 1 hit.
[Graphical view ]
PIRSFi PIRSF001604. LigA. 1 hit.
SMARTi SM00292. BRCT. 1 hit.
SM00532. LIGANc. 1 hit.
[Graphical view ]
SUPFAMi SSF47781. SSF47781. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 1 hit.
TIGRFAMsi TIGR00575. dnlj. 1 hit.
PROSITEi PS50172. BRCT. 1 hit.
PS01055. DNA_LIGASE_N1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-144 / Wilmington.

Entry informationi

Entry nameiDNLJ_RICTY
AccessioniPrimary (citable) accession number: Q68W27
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: October 11, 2004
Last modified: October 1, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia typhi
    Rickettsia typhi (strain Wilmington): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3