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Q68W27

- DNLJ_RICTY

UniProt

Q68W27 - DNLJ_RICTY

Protein

DNA ligase

Gene

ligA

Organism
Rickettsia typhi (strain ATCC VR-144 / Wilmington)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.UniRule annotation

    Catalytic activityi

    NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)(n+m).UniRule annotation

    Cofactori

    Magnesium or manganese.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei121 – 1211NADUniRule annotation
    Active sitei123 – 1231N6-AMP-lysine intermediateUniRule annotation
    Binding sitei144 – 1441NADUniRule annotation
    Binding sitei179 – 1791NADUniRule annotation
    Binding sitei295 – 2951NADUniRule annotation
    Binding sitei319 – 3191NADUniRule annotation
    Metal bindingi413 – 4131ZincUniRule annotation
    Metal bindingi416 – 4161ZincUniRule annotation
    Metal bindingi431 – 4311ZincUniRule annotation
    Metal bindingi437 – 4371ZincUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi40 – 445NADUniRule annotation
    Nucleotide bindingi89 – 902NADUniRule annotation

    GO - Molecular functioni

    1. DNA ligase (NAD+) activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. DNA repair Source: UniProtKB-KW
    2. DNA replication Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    DNA damage, DNA repair, DNA replication

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NAD, Zinc

    Enzyme and pathway databases

    BioCyciRTYP257363:GJEQ-743-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA ligaseUniRule annotation (EC:6.5.1.2UniRule annotation)
    Alternative name(s):
    Polydeoxyribonucleotide synthase [NAD(+)]UniRule annotation
    Gene namesi
    Name:ligAUniRule annotation
    Synonyms:lig
    Ordered Locus Names:RT0706
    OrganismiRickettsia typhi (strain ATCC VR-144 / Wilmington)
    Taxonomic identifieri257363 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
    ProteomesiUP000000604: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 693693DNA ligasePRO_0000280948Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi257363.RT0706.

    Structurei

    3D structure databases

    ProteinModelPortaliQ68W27.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini610 – 69384BRCTUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the NAD-dependent DNA ligase family. LigA subfamily.UniRule annotation
    Contains 1 BRCT domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0272.
    HOGENOMiHOG000218458.
    KOiK01972.
    OMAiKEYYVDS.
    OrthoDBiEOG6TTVM9.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    3.40.50.10190. 1 hit.
    HAMAPiMF_01588. DNA_ligase_A.
    InterProiIPR001357. BRCT_dom.
    IPR018239. DNA_ligase_AS.
    IPR001679. DNAligase.
    IPR013839. DNAligase_adenylation.
    IPR013840. DNAligase_N.
    IPR012340. NA-bd_OB-fold.
    IPR004150. NAD_DNA_ligase_OB.
    IPR010994. RuvA_2-like.
    IPR004149. Znf_DNAligase_C4.
    [Graphical view]
    PfamiPF00533. BRCT. 1 hit.
    PF01653. DNA_ligase_aden. 1 hit.
    PF03120. DNA_ligase_OB. 1 hit.
    PF03119. DNA_ligase_ZBD. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001604. LigA. 1 hit.
    SMARTiSM00292. BRCT. 1 hit.
    SM00532. LIGANc. 1 hit.
    [Graphical view]
    SUPFAMiSSF47781. SSF47781. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF52113. SSF52113. 1 hit.
    TIGRFAMsiTIGR00575. dnlj. 1 hit.
    PROSITEiPS50172. BRCT. 1 hit.
    PS01055. DNA_LIGASE_N1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q68W27-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQNIDLISEK EAKKLLEELA DKIAMYNHAY YIEDNPLVSD SEYDQLFNLN    50
    LKLENTFPHL VLSNSPSKKI GAKITNKFAK VIHQAPMLSL SNAFDEEDIR 100
    DFLERIKNFL RINEFTPIFC EPKIDGLSFS AIYKHGLFIT GATRGDGYVG 150
    EDITINIKTI KNFPHKIDNA PEFLEVRGEI YIEKQDFVNL NKEQEAQNRG 200
    KFANPRNAAA GSIRQLDVAI TAQRPLKYFI YSGGVTEKNL ASTQEQLLAK 250
    LKALSFSVNE ISKLASSEEE IFAFYEYLKT NRHNLPYEID GVVYKLNNFA 300
    MQNRMGFIAR SPRFAIAHKF PAIIGQTKLL SITVQVGRTG MLTPVAELDP 350
    LEIGGVVVSR ATLHNFQDIA RKDVRIKDYV FLQRAGDVIP QITGVDISKR 400
    SNDTVKFDTP VFCPSCNSKL RYVSEDIIIR CDNGLNCPAQ NYERICHFVS 450
    KNAMDIAGLG RKQVAFLIDK RLISNPLDIF FLKKKNETNL IRLENMDGWG 500
    KKSVANLWQN IEKSQKISLQ RFIYALGIRH IGEQNAKLLA REFVSYNNFI 550
    AQMELLSKND SDVYQKLNDL EGIGDKMLVD IIDFFYVKEN IQLIKKLGAV 600
    LNIEDYKETR EQNILTGKIL VFTGSLKTIS RVEAKEIAEK LGAKVASSVS 650
    SNTDLLIVGV NGGSKLKKAK ELNIKIIDEA EWLAFIKCLE KEV 693
    Length:693
    Mass (Da):78,483
    Last modified:October 11, 2004 - v1
    Checksum:i80BE9C9493DF658B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017197 Genomic DNA. Translation: AAU04165.1.
    RefSeqiYP_067647.1. NC_006142.1.

    Genome annotation databases

    EnsemblBacteriaiAAU04165; AAU04165; RT0706.
    GeneIDi2959338.
    KEGGirty:RT0706.
    PATRICi17910662. VBIRicTyp34752_0726.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017197 Genomic DNA. Translation: AAU04165.1 .
    RefSeqi YP_067647.1. NC_006142.1.

    3D structure databases

    ProteinModelPortali Q68W27.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 257363.RT0706.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAU04165 ; AAU04165 ; RT0706 .
    GeneIDi 2959338.
    KEGGi rty:RT0706.
    PATRICi 17910662. VBIRicTyp34752_0726.

    Phylogenomic databases

    eggNOGi COG0272.
    HOGENOMi HOG000218458.
    KOi K01972.
    OMAi KEYYVDS.
    OrthoDBi EOG6TTVM9.

    Enzyme and pathway databases

    BioCyci RTYP257363:GJEQ-743-MONOMER.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    3.40.50.10190. 1 hit.
    HAMAPi MF_01588. DNA_ligase_A.
    InterProi IPR001357. BRCT_dom.
    IPR018239. DNA_ligase_AS.
    IPR001679. DNAligase.
    IPR013839. DNAligase_adenylation.
    IPR013840. DNAligase_N.
    IPR012340. NA-bd_OB-fold.
    IPR004150. NAD_DNA_ligase_OB.
    IPR010994. RuvA_2-like.
    IPR004149. Znf_DNAligase_C4.
    [Graphical view ]
    Pfami PF00533. BRCT. 1 hit.
    PF01653. DNA_ligase_aden. 1 hit.
    PF03120. DNA_ligase_OB. 1 hit.
    PF03119. DNA_ligase_ZBD. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001604. LigA. 1 hit.
    SMARTi SM00292. BRCT. 1 hit.
    SM00532. LIGANc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47781. SSF47781. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF52113. SSF52113. 1 hit.
    TIGRFAMsi TIGR00575. dnlj. 1 hit.
    PROSITEi PS50172. BRCT. 1 hit.
    PS01055. DNA_LIGASE_N1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC VR-144 / Wilmington.

    Entry informationi

    Entry nameiDNLJ_RICTY
    AccessioniPrimary (citable) accession number: Q68W27
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 20, 2007
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Rickettsia typhi
      Rickettsia typhi (strain Wilmington): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3