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Q68VZ0

- MNME_RICTY

UniProt

Q68VZ0 - MNME_RICTY

Protein

tRNA modification GTPase MnmE

Gene

mnmE

Organism
Rickettsia typhi (strain ATCC VR-144 / Wilmington)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    • Comment

    Functioni

    Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34.UniRule annotation

    Cofactori

    Binds 1 potassium ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei20 – 201FormyltetrahydrofolateUniRule annotation
    Binding sitei79 – 791FormyltetrahydrofolateUniRule annotation
    Binding sitei119 – 1191FormyltetrahydrofolateUniRule annotation
    Metal bindingi225 – 2251PotassiumUniRule annotation
    Metal bindingi229 – 2291MagnesiumUniRule annotation
    Metal bindingi244 – 2441Potassium; via carbonyl oxygenUniRule annotation
    Metal bindingi246 – 2461Potassium; via carbonyl oxygenUniRule annotation
    Metal bindingi249 – 2491PotassiumUniRule annotation
    Metal bindingi250 – 2501MagnesiumUniRule annotation
    Binding sitei445 – 4451FormyltetrahydrofolateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi225 – 2306GTPUniRule annotation
    Nucleotide bindingi244 – 2507GTPUniRule annotation
    Nucleotide bindingi269 – 2724GTPUniRule annotation

    GO - Molecular functioni

    1. GTPase activity Source: UniProtKB-HAMAP
    2. GTP binding Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. tRNA modification Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    GTP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

    Enzyme and pathway databases

    BioCyciRTYP257363:GJEQ-785-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA modification GTPase MnmEUniRule annotation (EC:3.6.-.-UniRule annotation)
    Gene namesi
    Name:mnmEUniRule annotation
    Synonyms:trmEUniRule annotation
    Ordered Locus Names:RT0745
    OrganismiRickettsia typhi (strain ATCC VR-144 / Wilmington)
    Taxonomic identifieri257363 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
    ProteomesiUP000000604: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 445445tRNA modification GTPase MnmEPRO_0000278005Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer. Heterotetramer of two MnmE and two MnmG subunits.UniRule annotation

    Protein-protein interaction databases

    STRINGi257363.RT0745.

    Structurei

    3D structure databases

    ProteinModelPortaliQ68VZ0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini215 – 371157TrmE-type GAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0486.
    HOGENOMiHOG000200714.
    KOiK03650.
    OMAiCAETEWD.
    OrthoDBiEOG6DC6K1.

    Family and domain databases

    Gene3Di1.20.120.430. 3 hits.
    3.30.1360.120. 1 hit.
    3.40.50.300. 2 hits.
    HAMAPiMF_00379. GTPase_MnmE.
    InterProiIPR018948. GTP-bd_TrmE_N.
    IPR006073. GTP_binding_domain.
    IPR004520. GTPase_MnmE.
    IPR025867. GTPase_MnmE_C_dom.
    IPR008144. Guanylate_kin-like.
    IPR027368. MnmE_dom2.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR027266. TrmE/GcvT_dom1.
    [Graphical view]
    PfamiPF12631. GTPase_Cys_C. 1 hit.
    PF01926. MMR_HSR1. 1 hit.
    PF10396. TrmE_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    TIGRFAMsiTIGR00450. mnmE_trmE_thdF. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEiPS51709. G_TRME. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q68VZ0-1 [UniParc]FASTAAdd to Basket

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    METIFAQSSA FGKAGVAVFR ISGPKSLEVL QLLTGRKDFK PRLMYYQQII    50
    SPETNELIDN AMVVYFKLPN SFTGEDVVEI HTHGSKAISI MLINTLLNID 100
    DIRLAEAGEF TKRAFLNNKF DLTAAEGIAD LINAETIMQH RQAVRQANGG 150
    LEELYNNWRN QLLKIIALLE AYLDFPDEDI PDSILNDVNN THKNIVNEIS 200
    NYLNDNRRGE LLNNGLKLAI IGPPNTGKSS LLNFLMQRNI AIVSNIAGTT 250
    RDIIEGHLDI GGYPIILQDT AGIRAESTDI IEREGIKRAI NSAKTANIKI 300
    IMFDAEKLDL SINNDIIDLI DENTIVIINK IDLIEPSQIF PIEKKYKCLR 350
    VSVKNNIALS SILKNIENIA ENIAGFTETP YITNQRHRHY LKQALSHLMA 400
    FNLDNDLVLA TEDMRMTARC IGLITGVINV EEILNEIFKN FCIGK 445
    Length:445
    Mass (Da):49,892
    Last modified:October 11, 2004 - v1
    Checksum:i3E700EAC12F31B55
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017197 Genomic DNA. Translation: AAU04202.1.
    RefSeqiYP_067684.1. NC_006142.1.

    Genome annotation databases

    EnsemblBacteriaiAAU04202; AAU04202; RT0745.
    GeneIDi2958546.
    KEGGirty:RT0745.
    PATRICi17910742. VBIRicTyp34752_0765.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017197 Genomic DNA. Translation: AAU04202.1 .
    RefSeqi YP_067684.1. NC_006142.1.

    3D structure databases

    ProteinModelPortali Q68VZ0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 257363.RT0745.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAU04202 ; AAU04202 ; RT0745 .
    GeneIDi 2958546.
    KEGGi rty:RT0745.
    PATRICi 17910742. VBIRicTyp34752_0765.

    Phylogenomic databases

    eggNOGi COG0486.
    HOGENOMi HOG000200714.
    KOi K03650.
    OMAi CAETEWD.
    OrthoDBi EOG6DC6K1.

    Enzyme and pathway databases

    BioCyci RTYP257363:GJEQ-785-MONOMER.

    Family and domain databases

    Gene3Di 1.20.120.430. 3 hits.
    3.30.1360.120. 1 hit.
    3.40.50.300. 2 hits.
    HAMAPi MF_00379. GTPase_MnmE.
    InterProi IPR018948. GTP-bd_TrmE_N.
    IPR006073. GTP_binding_domain.
    IPR004520. GTPase_MnmE.
    IPR025867. GTPase_MnmE_C_dom.
    IPR008144. Guanylate_kin-like.
    IPR027368. MnmE_dom2.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR027266. TrmE/GcvT_dom1.
    [Graphical view ]
    Pfami PF12631. GTPase_Cys_C. 1 hit.
    PF01926. MMR_HSR1. 1 hit.
    PF10396. TrmE_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    TIGRFAMsi TIGR00450. mnmE_trmE_thdF. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51709. G_TRME. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC VR-144 / Wilmington.

    Entry informationi

    Entry nameiMNME_RICTY
    AccessioniPrimary (citable) accession number: Q68VZ0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 20, 2007
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Rickettsia typhi
      Rickettsia typhi (strain Wilmington): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3