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Protein

tRNA modification GTPase MnmE

Gene

mnmE

Organism
Rickettsia typhi (strain ATCC VR-144 / Wilmington)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34.UniRule annotation

Cofactori

K+UniRule annotationNote: Binds 1 potassium ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei20FormyltetrahydrofolateUniRule annotation1
Binding sitei79FormyltetrahydrofolateUniRule annotation1
Binding sitei119FormyltetrahydrofolateUniRule annotation1
Metal bindingi225PotassiumUniRule annotation1
Metal bindingi229MagnesiumUniRule annotation1
Metal bindingi244Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi246Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi249PotassiumUniRule annotation1
Metal bindingi250MagnesiumUniRule annotation1
Binding sitei445FormyltetrahydrofolateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi225 – 230GTPUniRule annotation6
Nucleotide bindingi244 – 250GTPUniRule annotation7
Nucleotide bindingi269 – 272GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA modification GTPase MnmEUniRule annotation (EC:3.6.-.-UniRule annotation)
Gene namesi
Name:mnmEUniRule annotation
Synonyms:trmEUniRule annotation
Ordered Locus Names:RT0745
OrganismiRickettsia typhi (strain ATCC VR-144 / Wilmington)
Taxonomic identifieri257363 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
Proteomesi
  • UP000000604 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002780051 – 445tRNA modification GTPase MnmEAdd BLAST445

Interactioni

Subunit structurei

Homodimer. Heterotetramer of two MnmE and two MnmG subunits.UniRule annotation

Protein-protein interaction databases

STRINGi257363.RT0745.

Structurei

3D structure databases

ProteinModelPortaliQ68VZ0.
SMRiQ68VZ0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini215 – 371TrmE-type GAdd BLAST157

Sequence similaritiesi

Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C1H. Bacteria.
COG0486. LUCA.
HOGENOMiHOG000200714.
KOiK03650.
OMAiFTPRYAY.
OrthoDBiPOG091H01GD.

Family and domain databases

CDDicd04164. trmE. 1 hit.
Gene3Di1.20.120.430. 3 hits.
3.30.1360.120. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00379. GTPase_MnmE. 1 hit.
InterProiIPR031168. G_TrmE.
IPR018948. GTP-bd_TrmE_N.
IPR006073. GTP_binding_domain.
IPR004520. GTPase_MnmE.
IPR008144. Guanylate_kin-like_dom.
IPR027368. MnmE_dom2.
IPR025867. MnmE_helical.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamiPF01926. MMR_HSR1. 1 hit.
PF12631. MnmE_helical. 1 hit.
PF10396. TrmE_N. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00450. mnmE_trmE_thdF. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51709. G_TRME. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q68VZ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METIFAQSSA FGKAGVAVFR ISGPKSLEVL QLLTGRKDFK PRLMYYQQII
60 70 80 90 100
SPETNELIDN AMVVYFKLPN SFTGEDVVEI HTHGSKAISI MLINTLLNID
110 120 130 140 150
DIRLAEAGEF TKRAFLNNKF DLTAAEGIAD LINAETIMQH RQAVRQANGG
160 170 180 190 200
LEELYNNWRN QLLKIIALLE AYLDFPDEDI PDSILNDVNN THKNIVNEIS
210 220 230 240 250
NYLNDNRRGE LLNNGLKLAI IGPPNTGKSS LLNFLMQRNI AIVSNIAGTT
260 270 280 290 300
RDIIEGHLDI GGYPIILQDT AGIRAESTDI IEREGIKRAI NSAKTANIKI
310 320 330 340 350
IMFDAEKLDL SINNDIIDLI DENTIVIINK IDLIEPSQIF PIEKKYKCLR
360 370 380 390 400
VSVKNNIALS SILKNIENIA ENIAGFTETP YITNQRHRHY LKQALSHLMA
410 420 430 440
FNLDNDLVLA TEDMRMTARC IGLITGVINV EEILNEIFKN FCIGK
Length:445
Mass (Da):49,892
Last modified:October 11, 2004 - v1
Checksum:i3E700EAC12F31B55
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017197 Genomic DNA. Translation: AAU04202.1.
RefSeqiWP_011191178.1. NC_006142.1.

Genome annotation databases

EnsemblBacteriaiAAU04202; AAU04202; RT0745.
KEGGirty:RT0745.
PATRICi17910742. VBIRicTyp34752_0765.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017197 Genomic DNA. Translation: AAU04202.1.
RefSeqiWP_011191178.1. NC_006142.1.

3D structure databases

ProteinModelPortaliQ68VZ0.
SMRiQ68VZ0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi257363.RT0745.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU04202; AAU04202; RT0745.
KEGGirty:RT0745.
PATRICi17910742. VBIRicTyp34752_0765.

Phylogenomic databases

eggNOGiENOG4105C1H. Bacteria.
COG0486. LUCA.
HOGENOMiHOG000200714.
KOiK03650.
OMAiFTPRYAY.
OrthoDBiPOG091H01GD.

Family and domain databases

CDDicd04164. trmE. 1 hit.
Gene3Di1.20.120.430. 3 hits.
3.30.1360.120. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00379. GTPase_MnmE. 1 hit.
InterProiIPR031168. G_TrmE.
IPR018948. GTP-bd_TrmE_N.
IPR006073. GTP_binding_domain.
IPR004520. GTPase_MnmE.
IPR008144. Guanylate_kin-like_dom.
IPR027368. MnmE_dom2.
IPR025867. MnmE_helical.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamiPF01926. MMR_HSR1. 1 hit.
PF12631. MnmE_helical. 1 hit.
PF10396. TrmE_N. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00450. mnmE_trmE_thdF. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51709. G_TRME. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMNME_RICTY
AccessioniPrimary (citable) accession number: Q68VZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: October 11, 2004
Last modified: November 2, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia typhi
    Rickettsia typhi (strain Wilmington): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.