Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA modification GTPase MnmE

Gene

mnmE

Organism
Rickettsia typhi (strain ATCC VR-144 / Wilmington)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34.UniRule annotation

Cofactori

K+UniRule annotationNote: Binds 1 potassium ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei20FormyltetrahydrofolateUniRule annotation1
Binding sitei79FormyltetrahydrofolateUniRule annotation1
Binding sitei119FormyltetrahydrofolateUniRule annotation1
Metal bindingi225PotassiumUniRule annotation1
Metal bindingi229MagnesiumUniRule annotation1
Metal bindingi244Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi246Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi249PotassiumUniRule annotation1
Metal bindingi250MagnesiumUniRule annotation1
Binding sitei445FormyltetrahydrofolateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi225 – 230GTPUniRule annotation6
Nucleotide bindingi244 – 250GTPUniRule annotation7
Nucleotide bindingi269 – 272GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processtRNA processing
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

BioCyciRTYP257363:G1G0L-753-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA modification GTPase MnmEUniRule annotation (EC:3.6.-.-UniRule annotation)
Gene namesi
Name:mnmEUniRule annotation
Synonyms:trmEUniRule annotation
Ordered Locus Names:RT0745
OrganismiRickettsia typhi (strain ATCC VR-144 / Wilmington)
Taxonomic identifieri257363 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
Proteomesi
  • UP000000604 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002780051 – 445tRNA modification GTPase MnmEAdd BLAST445

Interactioni

Subunit structurei

Homodimer. Heterotetramer of two MnmE and two MnmG subunits.UniRule annotation

Protein-protein interaction databases

STRINGi257363.RT0745

Structurei

3D structure databases

ProteinModelPortaliQ68VZ0
SMRiQ68VZ0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini215 – 371TrmE-type GAdd BLAST157

Sequence similaritiesi

Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C1H Bacteria
COG0486 LUCA
HOGENOMiHOG000200714
KOiK03650
OMAiEFTQRAF
OrthoDBiPOG091H01GD

Family and domain databases

CDDicd04164 trmE, 1 hit
Gene3Di1.20.120.430, 2 hits
3.30.1360.120, 1 hit
HAMAPiMF_00379 GTPase_MnmE, 1 hit
InterProiView protein in InterPro
IPR031168 G_TrmE
IPR018948 GTP-bd_TrmE_N
IPR006073 GTP_binding_domain
IPR004520 GTPase_MnmE
IPR008144 Guanylate_kin-like_dom
IPR027368 MnmE_dom2
IPR025867 MnmE_helical
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR027266 TrmE/GcvT_dom1
PfamiView protein in Pfam
PF01926 MMR_HSR1, 1 hit
PF12631 MnmE_helical, 1 hit
PF10396 TrmE_N, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
TIGRFAMsiTIGR00450 mnmE_trmE_thdF, 1 hit
TIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51709 G_TRME, 1 hit

Sequencei

Sequence statusi: Complete.

Q68VZ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METIFAQSSA FGKAGVAVFR ISGPKSLEVL QLLTGRKDFK PRLMYYQQII
60 70 80 90 100
SPETNELIDN AMVVYFKLPN SFTGEDVVEI HTHGSKAISI MLINTLLNID
110 120 130 140 150
DIRLAEAGEF TKRAFLNNKF DLTAAEGIAD LINAETIMQH RQAVRQANGG
160 170 180 190 200
LEELYNNWRN QLLKIIALLE AYLDFPDEDI PDSILNDVNN THKNIVNEIS
210 220 230 240 250
NYLNDNRRGE LLNNGLKLAI IGPPNTGKSS LLNFLMQRNI AIVSNIAGTT
260 270 280 290 300
RDIIEGHLDI GGYPIILQDT AGIRAESTDI IEREGIKRAI NSAKTANIKI
310 320 330 340 350
IMFDAEKLDL SINNDIIDLI DENTIVIINK IDLIEPSQIF PIEKKYKCLR
360 370 380 390 400
VSVKNNIALS SILKNIENIA ENIAGFTETP YITNQRHRHY LKQALSHLMA
410 420 430 440
FNLDNDLVLA TEDMRMTARC IGLITGVINV EEILNEIFKN FCIGK
Length:445
Mass (Da):49,892
Last modified:October 11, 2004 - v1
Checksum:i3E700EAC12F31B55
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017197 Genomic DNA Translation: AAU04202.1
RefSeqiWP_011191178.1, NC_006142.1

Genome annotation databases

EnsemblBacteriaiAAU04202; AAU04202; RT0745
KEGGirty:RT0745

Similar proteinsi

Entry informationi

Entry nameiMNME_RICTY
AccessioniPrimary (citable) accession number: Q68VZ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: October 11, 2004
Last modified: March 28, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health