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Q68VZ0 (MNME_RICTY) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA modification GTPase MnmE
EC=3.6.-.-
Gene names
Name:mnmE
Synonyms:trmE
Ordered Locus Names:RT0745
OrganismRickettsia typhi (strain ATCC VR-144 / Wilmington) [Complete proteome] [HAMAP]
Taxonomic identifier257363 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group ›

Protein attributes

Sequence length445 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity. HAMAP-Rule MF_00379

Cofactor

Binds 1 potassium ion per subunit By similarity.

Subunit structure

Homodimer By similarity. Heterotetramer of two MnmE and two MnmG subunits By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the Era/MnmE GTP-binding protein family. MnmE subfamily.

Contains 1 G (guanine nucleotide-binding) domain.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandGTP-binding
Magnesium
Metal-binding
Nucleotide-binding
Potassium
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA modification

Inferred from electronic annotation. Source: HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: HAMAP

GTPase activity

Inferred from electronic annotation. Source: HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 445445tRNA modification GTPase MnmE HAMAP-Rule MF_00379
PRO_0000278005

Regions

Domain227 – 330104G
Nucleotide binding225 – 2306GTP By similarity
Nucleotide binding244 – 2507GTP By similarity
Nucleotide binding269 – 2724GTP By similarity

Sites

Metal binding2251Potassium By similarity
Metal binding2291Magnesium By similarity
Metal binding2441Potassium; via carbonyl oxygen By similarity
Metal binding2461Potassium; via carbonyl oxygen By similarity
Metal binding2491Potassium By similarity
Metal binding2501Magnesium By similarity
Binding site201Formyltetrahydrofolate By similarity
Binding site791Formyltetrahydrofolate By similarity
Binding site1191Formyltetrahydrofolate By similarity
Binding site4451Formyltetrahydrofolate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q68VZ0 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 3E700EAC12F31B55

FASTA44549,892
        10         20         30         40         50         60 
METIFAQSSA FGKAGVAVFR ISGPKSLEVL QLLTGRKDFK PRLMYYQQII SPETNELIDN 

        70         80         90        100        110        120 
AMVVYFKLPN SFTGEDVVEI HTHGSKAISI MLINTLLNID DIRLAEAGEF TKRAFLNNKF 

       130        140        150        160        170        180 
DLTAAEGIAD LINAETIMQH RQAVRQANGG LEELYNNWRN QLLKIIALLE AYLDFPDEDI 

       190        200        210        220        230        240 
PDSILNDVNN THKNIVNEIS NYLNDNRRGE LLNNGLKLAI IGPPNTGKSS LLNFLMQRNI 

       250        260        270        280        290        300 
AIVSNIAGTT RDIIEGHLDI GGYPIILQDT AGIRAESTDI IEREGIKRAI NSAKTANIKI 

       310        320        330        340        350        360 
IMFDAEKLDL SINNDIIDLI DENTIVIINK IDLIEPSQIF PIEKKYKCLR VSVKNNIALS 

       370        380        390        400        410        420 
SILKNIENIA ENIAGFTETP YITNQRHRHY LKQALSHLMA FNLDNDLVLA TEDMRMTARC 

       430        440 
IGLITGVINV EEILNEIFKN FCIGK

« Hide

References

[1]"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae."
McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. expand/collapse author list , Hong C., Yu X.-J., Walker D.H., Weinstock G.M.
J. Bacteriol. 186:5842-5855(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE017197 Genomic DNA. Translation: AAU04202.1.
RefSeqYP_067684.1. NC_006142.1.

3D structure databases

HSSPHSSP built from PDB template 1XZP based on UniProtKB Q9WYA4.
ProteinModelPortalQ68VZ0.
ModBaseSearch...

Protein-protein interaction databases

STRING257363.RT0745.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU04202; AAU04202; RT0745.
GeneID2958546.
KEGGrty:RT0745.
PATRIC17910742. VBIRicTyp34752_0765.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0486.
HOGENOMHOG000200714.
KOK03650.
OMAPGEFSEQ.
ProtClustDBPRK05291.

Enzyme and pathway databases

BioCycRTYP257363:GJEQ-785-MONOMER.

Family and domain databases

Gene3D3.30.1360.120. 1 hit.
HAMAPMF_00379. GTPase_MnmE.
InterProIPR018948. GTP-bd_TrmE_N.
IPR006073. GTP_binding_domain.
IPR004520. GTPase_MnmE.
IPR025867. GTPase_MnmE_C_dom.
IPR008144. Guanylate_kin.
IPR005225. Small_GTP-bd_dom.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamPF12631. GTPase_Cys_C. 1 hit.
PF01926. MMR_HSR1. 1 hit.
PF10396. TrmE_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR00450. mnmE_trmE_thdF. 1 hit.
TIGR00231. small_GTP. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMNME_RICTY
AccessionPrimary (citable) accession number: Q68VZ0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: October 11, 2004
Last modified: May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Rickettsia typhi

Rickettsia typhi (strain Wilmington): entries and gene names

SIMILARITY comments

Index of protein domains and families

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